Four turtle taxa are previously documented from the Cenomanian Arlington Archosaur Site (AAS) of the Lewisville Formation (Woodbine Group) in Texas. Herein, we describe a new side-necked turtle (Pleurodira), Pleurochayah appalachius gen. et sp. nov., which is a basal member of the Bothremydidae. Pleurochayah appalachius gen. et sp. nov. shares synapomorphic characters with other bothremydids, including shared traits with Kurmademydini and Cearachelyini, but has a unique combination of skull and shell traits. The new taxon is significant because it is the oldest crown pleurodiran turtle from North America and Laurasia, predating bothremynines Algorachelus peregrinus and Paiutemys tibert from Europe and North America respectively. This discovery also documents the oldest evidence of dispersal of crown Pleurodira from Gondwana to Laurasia. Pleurochayah appalachius gen. et sp. nov. is compared to previously described fossil pleurodires, placed in a modified phylogenetic analysis of pelomedusoid turtles, and discussed in the context of pleurodiran distribution in the mid-Cretaceous. Its unique combination of characters demonstrates marine adaptation and dispersal capability among basal bothremydids.
Pleurodira, colloquially known as “side-necked” turtles, form one of two major clades of turtles known from the Early Cretaceous to present1,2. Pleurodires are Gondwanan in origin, with the oldest unambiguous crown pleurodire dated to the Barremian in the Early Cretaceous2. Pleurodiran fossils typically come from relatively warm regions, and have a more limited distribution than Cryptodira (hidden-neck turtles)3,4,5,6. Living pleurodires are restricted to tropical regions once belonging to Gondwana7,8. The separation of Laurasia and Gondwana and subsequent fragmentation of their landmasses in the Early Cretaceous, along with variations in global temperatures, affected the distribution of some pleurodire lineages3,9.
One pleurodiran lineage, Bothremydodda (Taphrosphyini + Bothremydini, sensu Gaffney et al.10) is the only published pleurodiran lineage to disperse from Gondwana to Laurasia in the Cretaceous5. The oldest genus of Bothremydidae found in Laurasian deposits is Algorachelus8. Algorachelus peregrinus is found in coastal environments at the southern Laurasian middle-late Cenomanian sites of Algora, Guadalajara, Castile and Leon, Spain and Nazaré, Portugal8,9,11. Algorachelus parvus is represented in the early or middle Cenomanian of Palestine12. Finally, Paiutemys tibert is known from brackish uppermost Cenomanian deposits of the Laramidian Naturitas Formation in Utah, USA9,13. While some authors have subsumed P. tibert within Algorachelus9, Hermanson et al.14 did not support the position of Paiutemys tibert within the genus Algorachelus. Paiutemys tibert is not closely related to any other North American bothremydid, and represents a first and independent dispersal of Bothremydini to the continent9,13. Thus, including the new taxon described here, Bothremydidae from Gondwana reached the east coast of the Atlantic Ocean and Appalachia at least in the middle Cenomanian, and Laramidia at least in the late Cenomanian.
Here, we describe Pleurochayah appalachius, gen. et sp. nov. (Pleurodira: Bothremydidae), from the Arlington Archosaur Site—the second and earliest pleurodiran lineage found in Laurasian deposits. Pleurochayah appalachius gen. et sp. nov. has a unique combination of characters, including many shared with Kurmademydini and Cearachelyini, as well as some traits shared with later derived bothremydids, Bothremydini and Taphrosphyini. In addition to cranial, shell, and postcranial anatomy, we also describe and analyze the shell microanatomy of Pleurochayah appalachius gen. et sp. nov. and compare it to histology from related taxa.
The fossils derive from the Arlington Archosaur Site (AAS) in sediments most likely deposited near the middle of the Lewisville Formation [Fm] of the Upper Cretaceous Woodbine Group. The Woodbine Group [Gp] (lower middle Cenomanian) stretches from Temple in central Texas to Lake Texoma in southern Oklahoma, and is the oldest known unit in the Gulf Coastal Plain15,16,17,18,19,20,21 (Fig. 1a). The Woodbine Group sits unconformably between the older Grayson Marl of the Washita Group and the younger Eagle Ford Group17,18,20 (Fig. 1). At least ten million years of marine deposition separate the Woodbine Gp from the earlier terrestrial depositional systems of the Lower Cretaceous Trinity Gp22 (Fig. 1a). The minimum age for the of Upper Woodbine Gp and Tarrant Fm of the lower Eagle Ford Gp is 95–96 Ma, established by presence of Conlinoceras tarrantense, a zonal marker for the base of the middle Cenomanian20,21,23,24,25. The Woodbine Group comprises two Formations: the Dexter Fm overlain by the Lewisville Fm26 (Fig. 1a). The four lithologic units comprising the Lewisville Fm, from oldest to youngest are the Red Branch, an unnamed interval, Arlington, and Tarrant Members [Mbrs]26. The AAS deposits containing the fossils described herein most likely lie in the interval between the Arlington and marine Red Branch Mbrs26. All specimens described herein were found unassociated. See “Supplementary Information” for further geological background.
Pleurodira Cope, 186532.
Pelomedusoides Broin, 19883.
Bothremydidae Baur, 189133.
Pleurochayah LSID: urn:lsid:zoobank.org:act:5D12B06D-9E3F-496C-A842-585F73AFAD68 gen. nov.
Type: DMNH 2013-07-1782, a partial cranium consisting of most of the basicranium, neurocranium, right otic region, and portion of the rostrum.
Type locality and horizon: Cenomanian, Upper Cretaceous. Lewisville Fm, Woodbine Gp (Fig. 1). The Arlington Archosaur Site, city of Arlington, Tarrant County, Texas. Exact locality data are on file at the Perot Museum of Nature and Science, Dallas, Texas.
Etymology: “Pleuro” is Greek for “side”, with “Cha’yah” for “turtle” in the language of the Caddo, a Native American tribe that inhabited much of the Gulf Coast of North America. Species name refers to Appalachia, the region comprising eastern North America.
Referred material: DMNH 2013-07-0683, neural 3 (cf. juvenile) (Fig. 3a–d); DMNH 2013-07-1998, pygal (Fig. 3e–h); DMNH 2013-07-0517, left costal 1 (Fig. 3i–l); DMNH 2013-07-1999, left costal 5 (Fig. 3m–p); DMNH 2013-07-1320, partial cf. right costal 3 (Fig. 3q–t); DMNH 2013-07-0557, partial cf. right costal 3 (Fig. 3u–x); DMNH 2013-07-1405, left costal 6 (Fig. 3y–ab); DMNH 2013-07-0525, partial cf. left costal 4 (Fig. 3ac–af); DMNH 2013-07-1320, partial cf. right costal 4 (Fig. 3ag–aj); DMNH 2013-07-0673, right peripheral 7 (Fig. 3ak–ap); DMNH 2013-07-1279, left peripheral 9 (Fig. 3aq–at); DMNH 2013-07-0500, proximal left humerus (Fig. 4a–g).
Diagnosis: Pleurochayah appalachius gen. et sp. nov. is diagnosed as a pleurodire by its quadrate with ventral process extending medially to the braincase below the cranioquadrate space, and a condylus mandibularis positioned anterior to the basioccipital-basisphenoid suture. We attribute it to Pelomedusoides by the absence of parietal-squamosal contact, and to Podocnemidoidea by a quadrate-basioccipital contact, and a prootic that is covered in ventral view. P. appalachius gen. et sp. nov. is a representative of Bothremydidae based on its basisphenoid-quadrate contact, a (short) quadrate-supraoccipital contact, a large exoccipital-quadrate contact, and the absence of a fossa precolumellaris. P. appalachius gen. et sp. nov. has the following unique combination of primitive and derived cranial characters: moderate antrum post-oticum (shared with Kurmademydini and Cearachelyini); incisura columella auris fully open and confluent with sulcus eustachii; present, but incipient, supraoccipital-quadrate contact; subtriangular, anteriorly-pointing basisphenoid; moderate posterior temporal emargination; foramen stapediotemporale located anteriorly on otic capsule, but not adjacent to foramen nervi trigemini; moderately-sized fossa pterygoidea; short parietals with uniform width; undivided fenestra postotica; external cortex of carapace significantly thicker than internal; dorsally-oriented orbits; a short quadrate-supraoccipital contact (as in Kurmademydini); distantly separate foramen stapediotemporale and foramen nervi trigemini; and lack of a slit-like fenestra postotica. See “Supplementary Information” for full anatomical description.
See “Supplementary Information” for full histological description. The results of our histological analysis are largely consistent with other known bothremydid taxa, which have a significantly thicker external than internal cortex45,46. The predominance of woven bone suggests fast skeletal growth, a derived characteristic seen in C. placidoi and the extant pleurodiran genus Phrynops46,47. In contrast, extinct and extant cryptodires and the Cretaceous chelid Linderochelys rinconensis exhibit greater parallel-fibered bone, characteristic of a slower growth rate and a more conservative osteohistological pattern46,48,49,50,51,52,53.
In the phylogenetic analysis, twenty minimum length trees of 1513 steps were obtained. In the strict consensus tree, P. appalachius was positioned at the base of the Bothremydidae in an unresolved polytomy with Kinkonychelys rogersi54, Sankuchemys sethnai55, and Kurmademys kallamedensis56 (Fig. 6). In 16 of the minimum trees, P. appalachius fell as the basal-most member of the Bothremydidae; while in the other four trees, it fell within the Kurmademydini as its basal-most member. See “Supplementary Information” for Character State Analysis and full character matrix.
Comparison of the Pleurochayah appalachius gen. et sp. nov. cranium with other bothremydids
Pleurochayah appalachius gen. et sp. nov. has a unique combination of characters that differentiate it from other bothremydid taxa. Primarily, it displays many simplesiomoprhic characters that are found in Kurmademydini and Cearachelyini, but not in derived bothremydids such as Bpthremydini and taphrosphyini. Supraoccipital-quadrate contact is present but reduced compared to most other bothremydids, similar to Kurdamemys. The degree of posterior temporal emargination is moderate. The antrum postoticum is moderately sized, as in Kurmademydini. The foramina stapediotemporale and nervi trigemini are distant as in Kurmademydini and Cearachelyini, in contrast to the closer foramina diagnostic of Bothremydini + Taphrosphyni. The fossa precolumellaris is absent as in other early bothremydids. The quadrate process of the pterygoid is reduced compared to later bothremydids. The interorbital distance is wide, similar to that of bothremydines and Galianemys spp. The tympanic opening is elongated and oval. It lacks the slit-like fenestra postotica that characterizes cearachelyines.
Pleurochayah appalachius gen. et sp. nov. shares some traits with the contemporaneous A. peregrinus; however, most shared characters are plesiomorphic for bothremydids. The two taxa differ in many other phylogenetically significant characters. In general, the crania of both taxa are wide relative to their lengths, although this trait is common among bothremydids, and P. appalachius is closer in proportions to the slightly narrower Cearachelys than to the wider A. peregrinus. Prefrontal-parietal contacts are absent (as in many bothremydids). Both exhibit a short supraoccipital-quadrate contact, such that the prootic does not contact the opisthotic, as in many bothremydids. The two taxa lack a fossa precolumellaris and possess an incisura columella auris that is not completely closed, although the feature is substantially more open in P. appalachius gen. et sp. nov. Unlike A. peregrinus, the orbits in P. appalachius gen. et sp. nov. are more dorsally than laterally positioned. A lateral, more vertical orientation of the orbits is considered primitive among turtles, and the most common state for bothremydines10. However, dorsal and more horizontally oriented orbits are a synapomorphy for Bothremys spp. and Chedighaii sp.10. There is not a clear functional distinction between the lateral and dorsal positions of the orbits, though in chelonioids it has been hypothesized that dorsal orientation is associated with shallow-water and/or benthic habits, and vertical orientation with pelagic adaptation57.
The fossa orbitalis is posteriorly enlarged in A. peregrinus, but not in P. appalachius gen. et sp. nov., in which the septum orbitale is only slightly posterior to the orbital rim. There is extensive contact between the postorbital and frontal in A. peregrinus, but only a minimal point of contact in P. appalachius gen. et sp. nov. The foramen stapediotemporale is located further from the foramen nervi trigemini in P. appalachius gen. et sp. nov., although in both taxa the foramen stapediotemporale is positioned anteriorly on the otic chamber. Pleurochayah appalachius gen. et sp. nov. has a short crista supraoccipitalis, as opposed to the long crista supraoccipitalis in A. peregrinus.
Using ratios of basal skull width to other measures, Fig. 7 compares general skull proportions of a selection of bothremydid taxa. The ratio of the overall width of the skull to basal skull width in P. appalachius gen. et sp. nov. is 0.87, similar to the cearachelyine C. placidoi, which falls between the narrow Taphrosphyini (< 0.82) and broader Bothremydini and K. kallamedensis (> 0.97) (Fig. 7). The preorbital length to basal skull width ratio of P. appalachius gen. et sp. nov. is also quite low, embedded in a cluster with cearachelyines, K. kallamedensis, and F. mechinorum (Fig. 7). In the ratio of interorbital width to basal skull width, P. appalachius gen. et sp. nov. falls within the known taphrosphyine and cearachelyine ranges (Fig. 7). A similar intermediate position within the bothremydine spectrum of variation, is repeated in the height to basal skull width ratio (Fig. 7). However, P. appalachius gen. et sp. nov. could not be included in the final comparison due to its height being diagenetically altered by crushing. In summary, compared to other bothremydids known from skulls, P. appalachius gen. et sp. nov. has a moderately narrow skull with a very short face and moderately spaced orbits (Fig. 7).
Humeral comparison between Pleurochayah appalachius gen. et sp. nov. and other pleurodirans
Previous studies used humeral morphology to infer locomotor modes and performance41,59,60,61,62 and paleoecology63 in turtles. Figure 4 provides a comparison of all published fossil pleurodiran humeri, along with a few extant taxa. The proximal humerus of pleurodires generally has a hemispherical head that is slightly separated from the lateral and medial processes, but varies in proportional length and width, and in orientation relative to the humeral shaft10 (Fig. 4). A more spherical humeral head is considered the derived state in bothremydids10 (Fig. 4). The relative proportions of the humeral head (i.e., length to width) are nearly equal in P. appalachius gen. et sp. nov., similar to most specimens of Stupendemys spp., Taphrosphys sulcatus, and Hydromedusa tectifera (Fig. 4). In contrast, in Bothremys barberi, Bothremys sp., Chupacabrachelys complexus, and the extant Podocnemis expansa, the humeral head is slightly longer than wide, while in Notoemys laticentralis, the humeral head is wider than long (Fig. 4). The longitudinal axes of the humeral heads of P. appalachius gen. et sp. nov. and Taphrosphys sulcatus are parallel with the humeral shaft (Fig. 4). However, in Bothremys barberi, Bothremys sp., Chupacabrachelys complexus, Notoemys laticentralis, Podocnemis expansa, Hydromedusa tectifera, and Stupendemys spp., the longitudinal axis is tilted slightly laterally and the difference between the heights of the processes is generally greater, echoing this tilt (Fig. 4).
The lateral and medial humeral processes are homologous with the trochanter major and minor, respectively61. Usually, the medial process is taller and more robust than the lateral, and the difference in height varies significantly between taxa (Fig. 4). The medial process is only slightly taller than the lateral process in P. appalachius gen. et sp. nov., similar to Taphrosphys sulcatus, but the difference in heights is more notable in the remainder of the comparative sample (Fig. 4). The lateral process is shifted significantly distally in sea turtles and to a lesser degree in Carettochelys insculpta34. The humeral head of P. appalachius gen. et sp. nov. is taller than both processes, as in all compared taxa except the chelid Hydromedusa tectifera, Notoemys laticentralis, and most humeri of Stupendemys spp. (Fig. 4). The neck of the medial process is slightly depressed, forming a sloping saddle in all compared taxa except Notoemys laticentralis (Fig. 4).
The large proximal processes of semiaquatic turtle humeri increase the mechanical advantage of both the m. pectoralis major and m. latissimus dorsi supporting more powerful stroke and recovery during both aquatic rowing and terrestrial walking64,65,66. Muscles inserting on the proximal aspects of the lateral and medial processes act primarily to adduct and retract the humerus, while the more distal insertions are associated with protraction and abduction. In general, the lateral process tends to be associated with anterior movement of the limb, and the medial with posterior movement34. The lateral process is also relatively proximal compared to other sampled turtles, and is most similar to the marine-adapted Taphrosphys sulcatus (Fig. 4). Lateral and medial processes of similar height and size, as in P. appalachius gen. et sp. nov., suggests that adduction and retraction of the forelimb may be comparably distributed between the anterior and posterior directions, resulting in more equally powerful stroke and recovery motions during rowing. This is in contrast to an emphasis on posterior forelimb movement such as the flapping motion of many marine turtles usually associated with an enlarged medial process34.
The proximal humeral morphology of P. appalachius gen. et sp. nov., including a derived spherical head and robust medial and lateral processes similar to marine-adapted Taphrosphys sulcatus, suggests well-developed near-marine aquatic capabilities. Recovery of more skeletal material is needed to further test this intriguing hypothesis. While it should be noted that the described postcranial material was not found in direct association with the P. appalachius DMNH 2013-07-1782 cranium, we believe it to be the most parsimonious explanation that they belong to the same taxon, rather than the alternative that two previously undescribed pleurodiran species were present at AAS.
Histological comparison of Pleurochayah appalachius gen. et sp. nov. with fossil and extant pleurodires
In his seminal work on turtle shell histology, Scheyer45 developed a system of categories to quantify and relate histological characteristics and trends to the degree of aquatic adaptation of fossil and recent turtles. These categories are based on published ecological data of recent turtles67,68,69,70, and though a strict adherence to categorization should be treated with caution, a consideration of P. appalachius gen. et sp. nov. in the context of the analysis of Scheyer45 yields possible paleoecological insights into the new taxon.
Figure 8 compares the percentage thickness of the external and internal cortices relative to total shell bone thickness. P. appalachius gen. et sp. nov. falls closest to the marine-adapted bothremydids Chedighaii barberi and Taphrosphys sulcatus (Fig. 8). Comparative turtle taxa included here fall within Category II (semiaquatic to mainly aquatic) and Category III (fully aquatic) in degree of aquatic adaptation45. Categories II and III were combined into the Freshwater Histological Type of Jannello et al.71, which includes marine coastal environments. Turtles in Category II spend much of their lives in the water, often going onto land to migrate, forage, and bask45. They all have shell bones with a diploë microstructure, in which internal and external cortical bone layers frame interior cancellous bone45 (Fig. 8). The cortices are well developed, and the histological profile of the bone has a generally compact appearance45. Vascularization of the cortical bone is higher than terrestrial (Category I) turtles due to more primary vascular canals and secondary osteons45. Also, the transitions between the cortices and interior cancellous bone remain conspicuous45. Among the currently sampled taxa, the bothremydid Foxemys cf. mechinorum and chelid Emydura subglobosa belong to Category II, as do the sampled Podocnemididae (Stupendemys geographicus and Podocnemis erythrocephalica) and the pelomedusid Pelomedusa subrufa, though the latter also has tendencies toward Category I (Fig. 8). Turtles in Category III (the bothremydids Chedighaii barberi and Taphrosphys sulcatus) are characterized by: rarely leaving an aquatic environment to bask or lay eggs; reduction in compact bone layers in the shell; strong reduction in thickness of the internal cortex compared with the external cortex; strongly vascularized external cortex that is rarely reduced in thickness; and highly organized trabeculae in the cancellous bone45. A well-developed external cortex is hypothesized to ensure structural stability of the shell bones45. The clustering of P. appalachius gen. et sp. nov. with the Category III bothremydids (Fig. 8) is intriguing and suggests a highly aquatic, possibly semi-marine lifestyle. This functional interpretation is consistent with the morphology of the humerus.
Unlike the consistent account of aquatic adaptation shared by the sampled Podocnemididae, the bothremydid Foxemys cf. mechinorum shows less aquatic adaptation in its microanatomy than the marine bothremydid taxa Bothremys barberi and Taphrosphys sulcatus45 (Fig. 8). Also, P. appalachius gen. et sp. nov. has similar cortex to full shell thickness ratios as the marine taxa, further suggesting that it may also have been highly adapted to aquatic life (Fig. 8). Additional discoveries of shell and postcranial material may reveal further paleoecological insights63,73 on P. appalachius gen. et sp. nov. and related taxa. In particular, histological sampling of Algorachelus spp. to measure relative cortical thickness and other relevant characteristics may be useful in assessing the paleoecology of these taxa.
Pleurodiran distribution and southern Appalachian biogeography
As Kurmademydini is not retrieved in the current phylogenetic analysis, Pleurochayah appalachius gen. et sp. nov. is part of an unresolved basal bothremydid polytomy along with Sankuchemys sethnai55, Kinkonychelys rogersi54, and Kurmademys kallamedensis56 (Fig. 6). Basal bothremydids are known from Africa, South America, India, and now North America. The group originated in Gondwana during the early Cretaceous and secondarily colonized Europe and North America in the late Cretaceous13. The analysis of Joyce et al.13 implies that at least five separate clades invaded North America from Gondwana. In addition to the new taxon described herein, these migrations include the newly established ancestors of Paiutemys tibert prior to the Cenomanian, the previously established ancestors of Chedighaii and newly established ancestor of Chupacabrachelys complexus prior to the Campanian, the previously established ancestors of Bothremys cooki prior to the Maastrichtian, and the newly established ancestor of Taphrosphys sulcatus prior to the Paleocene13.
The vast majority of bothremydines are preserved in near-shore marine deposits surrounding the Atlantic Ocean10, while basal bothremydids are recovered from continental sediments2,10,13. Trans-Tethyan dispersal events (from northern Gondwana to the European archipelago) appear to be responsible for the movement of many faunal groups during the early Upper Cretaceous, particularly during the Cenomanian–Santonian interval74. The southwest coast of Appalachia has a similarly complex biogeographical system and lies along a land connection and migration route from Laramidia75. The AAS also occupies a unique geographical location near the opening of the Western Interior Seaway, which connected the Arctic to the Gulf of Mexico beginning in the late Albian31. As such, it was situated near a confluence of brackish and near-shore marine waters with continental drainages from the Ouachita Mountains to the north17. Marine adaptation was previously known as early as the Santonian among the Bothremydini, as Bothremys arabicus and Chedighaii sp. or Bothremys barberi likely had near-shore marine lifestyles76. Freshwater pleurodiran taxa experienced reduced diversity in the Albian to Campanian interval, while the littoral and marine taxa showed a trend of steadily increasing diversity over the same period77. The shell histology of P. appalachius gen. et sp. nov. provides an example of a basal bothremydid with comparable marine adaptation to bothremydines (Fig. 8), occurring near the beginning of the trend of increased marine diversity.
A partial adaptation of some bothremydid subclades to brackish and marine waters facilitated their dispersal and the invasion of different niches. More than vicariance or large-scale extinctions, dispersal events are considered the most important influence on biogeography in pleurodires77. Oceanic dispersals are known to be a significant cause of biogeographic range changes for other groups such as tortoises78,79, lizards80, amphibians81, and invertebrates82. Some pelomedusids and chelids have island distributions that suggest short dispersals77, and extant freshwater turtles Chelodina expansa and Emydura macquarii have been exposed to saline conditions for long periods (50 days) without physiological problems83. Consistent with this pattern of increased marine adapation in the mid-Cretaceous, Pleurochayah appalachius gen. et sp. nov. demonstrates gross morphological and paleohistological signatures of marine or highly aquatic brackish adaptations. This suggests that marine adaptations among stem-pleurodires were earlier and more widespread than previously thought77. In the context of the AAS paleoenvironment, P. appalachius gen. et sp. nov. joins a number of previously described brackish or marine-tolerant taxa including the crocodyliform Terminonaris, hybodont sharks, and multiple invertrebrates that are consistent with a costal setting where terrestrial, freshwater, and marine taxa intermingle20,84,85.
The current study places Pleurochayah appalachius gen. et sp. nov. at the base of the Bothremydidae, in an unresolved polytomy with the members of Kurmademydini (Fig. 6). It is the oldest known crown pleurodire and bothremydid from North America. This study also extends the geographic range of basal bothremydids to North America, which is significant since they were previously known only from South America prior to the Cenomanian (Fig. 9). Pleurochayah applachius gen. et sp. nov. is known from the lower middle Cenomanian, predating Algorachelus peregrinus in the uppermost middle-lowermost upper Cenomanian9, and Pauitemys tibert in the uppermost Cenomanian13, making it the oldest known pleurodire from North America and Laurasia. It is likely that bothremydid dispersals to North America during or prior to the Cenomanian were influenced by the vicariant event of the opening of the central Atlantic, and the periodic connection of the Western Interior Seaway to the Gulf of Mexico. However, allopatric speciation may be responsible for the multiple-continent distribution of basal bothremydids, as demonstrated for other late Early Cretaceous pelomedusoid clades. Recent discoveries of new bothremydid taxa in Central America86 suggest additional insight into circum-Carribbean pleurodiran migrations may be possible.
Materials and methods
Fossil specimens were collected and prepared by researchers, staff, and volunteers of the Perot Museum of Nature and Science. There were no definitive associations between the cranium and postcranial material. Specimens were measured with 6″ Mitutoyo Absolute Digimatic calipers to the nearest 0.01 mm and rounded to the nearest 0.1 mm. Some distances and angles were measured from high quality digital photographs using ImageJ88.
For our phylogenetic analysis of P. appalachius gen. et sp. nov., we used the recent character matrix of 285 characters from Hermanson et al.14 designed to reveal phylogenetic relationships within Pleurodira. Characters following a morphocline were ordered according to Hermanson et al.14. Phylogenetic analyses were performed in TNT 1.589. We conducted a traditional search using 1000 replicates of Wagner trees and tree bisection-reconnection (TBR) saving 100 trees per replication. The full character matrix derived from Hermanson et al.14 is available in “Supplementary Information”.
For histological thin-sectioning, DMNH 2013-07-0525 was left undecalcified and embedded in plastic resin following the protocol of Lee and Simons93. Slides were imaged using a motorized light microscope (Ni-U; Nikon, Tokyo, Japan, USA) with a strain-free long working distance objective (10× Plan Fluor: numerical aperture of 0.3, resolvable size ≈ 1 μm). Focus and stitching of histological montages were controlled by software (NIS Elements D; Nikon, Tokyo, Japan, USA). The montages were sharpened using Photoshop (CC; Adobe, San Jose, California, USA) with the “Unsharp Mask” filter set at 10 px and are high resolution (2.1 μm per pixel). To comply with major grant funding agencies and promote data transparency, we provide the images as freely accessible digital slides at the Paleohistology Repository (Lee and O’Connor94: http://paleohistology.appspot.com).
Joyce, W. G., Parham, J. F. & Gauthier, J. A. Developing a protocol for the conversion of rank-based taxon names to phylogenetically define clade names, as exemplified by turtles. J. Paleontol. 78, 989–1013. https://doi.org/10.1666/0022-3360(2004)078%3C0989:DAPFTC%3E2.0.CO;2 (2004).
Romano, P. S. R., Gallo, V., Ramos, R. R. C. & Antoniolli, L. Atolchelys lepida, a new side-necked turtle from the Early Cretaceous of Brazil and the age of crown Pleurodira. Biol. Let. 10, 20140290. https://doi.org/10.1098/rsbl.2014.0290 (2014).
Broin, F. D. Les tortues et le Gondwana. Examen des rapports entre le fractionnement du Gondwana et la dispersion géographique des tortues pleurodires à partir du Crétacé. Stvdia Geol. Salamanticensia Stvdia Palaeocheloniol. 2, 103–142 (1988).
Iverson, J. B. A Revised Checklist with Distribution Maps of the Turtles of the World (Privately Published, 1992).
de Broin, F. L. The oldest pre-Podocnemidid turtle (Chelonii, Pleurodira) from the early Cretaceous, Ceará state, Brasil, and its environment. Treballs del Museu de Geologia de Barcelona 9, 43–95 (2000).
Pérez-García, A. & de Broin, F. L. An update to the diachronous migration to Europe of several Gondwanan lineages of pleurodiran turtles. Sociedad Geol. de España North Meets South Abstract Book Gondwana 15, 137 (2014).
de la Fuente, M. S. North Gondwanan Pleurodiran Turtles in Origin, Evolution and Biogeographic History of South American Turtles (eds Juliana Sterli, Ignacio Maniel, & Marcelo S. De la Fuente) Ch. 5, 95–127. https://doi.org/10.1007/978-3-319-00518-8_5 (Springer International Publishing, 2014).
Pérez-Garciá, A. A new turtle taxon (Podocnemidoidea, Bothremydidae) reveals the oldest known dispersal event of the crown Pleurodira from Gondwana to Laurasia. J. Syst. Paleontol. https://doi.org/10.1080/14772019.2016.1228549 (2016).
Pérez-García, A. et al. A bothremydid from the middle Cenomanian of Portugal identified as one of the oldest pleurodiran turtles in Laurasia. Cretac. Res. 78, 61–70. https://doi.org/10.1016/j.cretres.2017.05.031 (2017).
Gaffney, E. S., Tong, H. & Meylan, P. A. Evolution of the side-necked turtles: The families Bothremydidae, Euraxemydidae, and Araripemydidae. Bull. Am. Mus. Nat. Hist. 300, 1–318. https://doi.org/10.1206/0003-0090(2006)300[1:EOTSTT]2.0.CO;2 (2006).
Pérez-García, A. First evidence of a bothremydid turtle (crown Pleurodira) in the middle Cretaceous of Castile and Leon (Spain). J. Iber. Geol. https://doi.org/10.1007/s41513-020-00146-9 (2020).
Pérez-García, A. New information on the Cenomanian bothremydid turtle Algorachelus based on new, well-preserved material from Spain. Fossil Record 21, 119–135 (2018).
Joyce, W. G., Lyson, T. R. & Kirkland, J. I. An early bothremydid (Testudines, Pleurodira) from the Late Cretaceous (Cenomanian) of Utah, North America. PeerJ 4, e2502. https://doi.org/10.7717/peerj.2502 (2016).
Hermanson, G., Iori, F. V., Evers, S. W., Langer, M. C. & Ferreira, G. S. A small podocnemidoid (Pleurodira, Pelomedusoides) from the Late Cretaceous of Brazil, and the innervation and carotid circulation of side-necked turtles. Pap. Palaeontol. https://doi.org/10.1002/spp2.1300 (2020).
Hedlund, R. W. Palynology of the Red Branch Member of the Woodbine Formation (Cenomanian), Bryan County, Oklahoma. Oklahoma Geol. Survey 112, 1–69 (1966).
Dodge, C. F. Stratigraphic nomenclature of the Woodbine Formation Tarrant County, Texas. Texas J. Sci. 21, 43–62 (1969).
Oliver, W. B. Depositional systems in the Woodbine Formation (Upper Cretaceous), northeast Texas: The University of Texas at Austin. Bureau Econ. Geol. Rep. Investig. 73, 28 (1971).
Johnson, R. O. Lithofacies and depositional environments of the Rush Creek Member of the Woodbine Formation (Gulfian) of North Central Texas Master's thesis, University of Texas (1974).
Trudel, P. Stratigraphic sequences and facies architecture of the Woodbine-Eagle Ford interval, Upper Cretaceous, North Central Texas Unpublished Masters thesis, Tarleton State University (1994).
Noto, C. R. Archosaur fossil localities in the Woodbine Formation (Cenomanian) of North-Central Texas in Society of Vertebrate Paleontology Annual Meeting (ed Christopher R. Noto) 38–51 (2015).
Adams, T. L., Noto, C. R. & Drumheller, S. A large neosuchian crocodyliform from the Upper Cretaceous (Cenomanian) Woodbine Formation of North Texas. J. Vertebr. Paleontol. 37, e1349776. https://doi.org/10.1080/02724634.2017.1349776 (2017).
Winkler, D., Jacobs, L., Lee, Y. & Murry, P. Sea level fluctuation and terrestrial faunal change in North Central Texas in Sixth Symposium on Mesozoic Terrestrial Ecosystems and Biota Short Papers (eds A. Sun & Y. Wang) (China Ocean Press, 1995).
Kennedy, W. J. & Cobban, W. A. Cenomanian ammonite faunas from the Woodbine Formation and lower part of the Eagle Ford Group, Texas. Palaeontology 33, 75–154 (1990).
Lee, Y.-N. The Archosauria from the Woodbine Formation (Cenomanian) in Texas. J. Paleontol. 71, 1147–1156. https://doi.org/10.1017/S0022336000036088 (1997).
Jacobs, L. L. & Winkler, D. A. Mammals, archosaurs, and the Early to Late Cretaceous transition in north-central Texas. Natl. Sci. Museum Monogr. 14, 253–280 (1998).
Denne, R. A. et al. Biostratigraphic and geochemical constraints on the stratigraphy and depositional environments of the Eagle Ford and Woodbine Groups of Texas in The Eagle Ford Shale: A Renaissance in US Oil Production Vol. 110 (ed J.A. Breyer) 1–86 (AAPG Memoir, 2016).
Winkler, D. A., Murry, P. A. & Jacobs, L. L. Vertebrate Paleontology of the Trinity Group, Lower Cretaceous of Central Texas (Institute for the Study of Earth and Man, SMU, 1989).
Jacobs, L. L. et al. in Late Cretaceous to Quarternary Strata and Fossils of Texas: Field Excursions Celebrating 125 Years of GSA and Texas Geology, GSA South-Central Section Meeting, Austin, Texas, April 2013 1–13 (Geological Society of America, 2013).
Barnes, V. E. et al. Geological Atlas of Texas, Dallas Sheet (Bureau of Economic Geology, University of Texas at Austin, 1972).
Strganac, C. Field Trip Overview in Early and Mid-Cretaceous Archosaur Localities of North-Central Texas. Fieldtrip Guidebook (ed Christopher R. Noto) 2–4 (Society of Vertebrate Paleontology Annual Meeting, 2015).
Blakey, R. C. Paleogeography and paleotectonics of the Western Interior Seaway, Jurassic-Cretaceous of North America. Search Discov. 30392, 1–17 (2014).
Cope, E. D. Third contribution to herpetology of tropical America. Proc. Acad. Natl. Sci. Phila. 1865, 185–198 (1865).
Baur, G. Notes on some little known American Fossil Tortoises. Proc. Acad. Natl. Sci. Phila. 13, 111–130 (1891).
Walker, J. W. F. The locomotor apparatus of Testudines in Biology of the Reptilia Vol. 4 (eds Gans, C. & Parsons, T.S.) Ch.1, 1–51 (Academic Press, 1973).
Krahl, A. et al. Humerus osteology, myology, and finite element structure analysis of Cheloniidae. Anat. Rec. 303, 2177–2191. https://doi.org/10.1002/ar24311 (2019).
Zangerl, R. Fieldiana. Geology Memoirs, no. 2 Vol. 3 (Chicago Natural History Museum, 1948).
Zug, G. R. Turtles of the Lee Creek Mine (Pliocene: North Carolina) in Geology and Paleontology of the Lee Creek Mine, North Carolina, III Vol. 90 Smithsonian Contributions to Paleobiology (eds Clayton E. Ray & David J. Bohaska) 203–218 (Smithsonian Institution Press, 2001).
Lehman, T. M. & Wick, S. L. Chupacabrachelys complexus, n. gen. n. sp. (Testudines: Bothremydidae), ffrom the Aguja Formation (Campanian) of West Texas. J. Vertebrate Paleontol. 30, 1709–1725. https://doi.org/10.1080/02724634.2010.520782 (2010).
Gaffney, E. S. A revision of the side-necked turtle Taphrosphys sulcatus (Leidy) from the Cretaceous of New Jersey. Am. Mus. Novit. 2571, 1–24 (1975).
Fernandez, M. S. & de la Fuente, M. S. Redescription and phylogenetic position of Notoemys: The oldest Gondwanan pleurodiran turtle. N. Jb. Geol. Palaont. Abh. 193, 81–105 (1994).
Nakajima, Y., Hirayama, R. & Endo, H. Turtle humeral microanatomy and its relationship to lifestyle. Biol. J. Lin. Soc. 112, 719–734 (2014).
Bocquentin, J. & Melo, J. Stupdenemys souzi sp. nov. (Pleurodira, Podocnemididae) from the Miocene-Pliocene of the Solimoes Formation, Brazil. Revista Brasileira de Paleontologia 9, 187–192 (2006).
Lapparent de Broin, F., Bocquentin, J. & Negri, F. R. Gigantic turtles (Pleurodira, Podocnemididae) from the late Miocene-early Pliocene of southwestern Amazon. Bull. de l’Institut français d’études andines 22, 657–670 (1993).
Cadena, E. A. et al. The anatomy, paleobiology, and evolutionary relationships of the largest extinct side-necked turtle. Sci. Adv. 6, eaay4593 (2020).
Scheyer, T. M. Comparative bone histology of the turtle shell (carapace and plastron): implications for turtle systematics, functional morphology and turtle origins PhD thesis, University of Bonn (2007).
Sena, M. V. d. A., Bantim, R. A. M., Saraiva, A. Á. F., Sayão, J. M. & Oliveira, G. R. Osteohistology and microanatomy of a new specimen of Cearachelys placidoi (Testudines: Pleurodira) a side-necked turtle from the Lower Cretaceous of Brazil. Anat Rec., 1–11. https://doi.org/10.1002/ar.24556 (2020).
Enlow, D. H. The Bone of Reptiles in Biology of the Reptilia, Vol. 1: Morphology (eds C. Gans, D.A. Bellairs, & S.T. Parsons) 45–80 (Academic Press, 1969).
Scheyer, T. M. & Sander, P. M. Shell bone histology indicates terrestrial palaeoecology of basal turtles. Proc. Royal Soc. B. (2007). https://doi.org/10.1098/rspb.2007.0499.
Scheyer, T. M., Syromyatnikova, E. V. & Danilov, I. C. Turtle shell bone and osteoderm histology of Mesozoic and Cenozoic stem-trionychian Adocidae and Nanhsiungchelyidae (Cryptodira: Adocusia) from Central Asia, Mongolia, and North America. Fossil Record 20, 69–85. https://doi.org/10.5194/fr-20-69-2017 (2017).
Skutschas, P. P., Boitsova, E. A., Cherepanov, G. O. & Danilov, I. C. Shell bone histology of the pan-carettochelyid turtle Kizulkumemys schultzi from the Upper Cretaceous of Uzbekistan and shell bone morphology transformations in the evolution of pan-trionychian turtles. Cretac. Res. 79, 171–181 (2017).
Danilov, I. C., Obraztsova, E. M., Boitsova, E. A. & Skutschas, P. P. Diversity of Middle Jurassic turtles from the Berezovsk Quarry Locality, Krasnoyarsk Region, Russia: morphological and histological study. J. Paleontol. 52, 188–200. https://doi.org/10.1134/S0031030118010070 (2018).
Jannello, J. M., Maniel, I. J., Previtera, E. & de la Fuente, M. S. Linderochelys rinconensis (Testduines: Pan-Chelidae) from the Upper Cretaceous of northern Patagonia: New insights from shell bone histology, morphology, and diagenetic implications. Cretac. Res. 83, 47–61 (2018).
Skutschas, P. P. et al. Basal turtle material from the Lower Cretaceous of Yakutia (Russia) filling the gap in the Asian record. Cretac. Res. 106, 104186 (2020).
Gaffney, E. S., Krause, D. W. & Zalmout, I. S. Kinkonychelys, a new side-necked turtle (Pelomedusoides: Bothremydidae) from the Late Cretaceous of Madagascar. Am. Mus. Novit. 3662, 1–25 (2009).
Gaffney, E. S., Sahni, A., Schleich, H., Singh, S. D. & Srivastava, R. Sankuchemys, a new side-necked turtle (Pelomedusoides: Bothremydidae). Am. Mus. Novit. 3405, 1–10 (2003).
Gaffney, E. S., Chatterjee, S. & Rudra, D. K. Kurmademys, a new side-necked turtle (Pelomedusoides: Bothremydidae) from the Late Cretaceous of India. Am. Mus. Novit. 3245, 1–12 (2001).
Kear, B. P. & Lee, M. S. Y. A primitive protostegid from Australia and early sea turtle evolution. Biol. Let. 2, 116–119. https://doi.org/10.1098/rsbl.2005.0406 (2006).
Gaffney, E. S., Tong, H. & Meylan, P. A. Galianemys, a new side-necked turtle (Pelomedusoides: Bothremydidae) from the Late Cretaceous of Morocco. Am. Mus. Novit. 3379, 1–20 (2002).
Hirayama, R. Humeral morphology of chelonioid sea-turtles; its functional analysis and phylogenetic implications. Bull. Hobetsu Museum 8, 17–57. https://doi.org/10.13140/2.1.1399.5208 (1992).
Claude, J., Paradis, E., Tong, H. & Auffray, J.-C. A geometric morphometric assessment of the effects of environment and cladogenesis on the evolution of the turtle shell. Biol. J. Lin. Soc. 79, 485–501 (2003).
Depecker, M., Berge, C., Penn, X. & Renous, S. Geometric morphometrics of the shoulder girdle in extant turtles (Chelonii). J. Anat. 208, 35–45 (2006).
Depecker, M., Renous, S., Penin, X. & Berge, C. Procrustes analysis: a tool to understand shape changes of the humerus in turtles (Chelonii). C.R. Palevol 5, 509–518 (2006).
Joyce, W. G. & Gauthier, J. A. Paleoecology of Triassic stem turtles sheds new light on turtle origins. Proc. R. Soc. B Biol. Sci. 271, 1–5. https://doi.org/10.1098/rspb.2003.2523 (2004).
Rivera, A. R. V. & Blob, R. W. Forelimb kinematics and morot patterns of the slider turtle (Trachemys scripta) during swimming and walking: shared and novel strategies for meeting locomotor demands of water and land. J. Exp. Biol. 213, 3515–3526 (2010).
Blob, R. W., Mayerl, C. J., Rivera, A. R. V., Rivera, G. & Young, V. K. H. “On the Fence” versus “All in”: Insights from turtles for the evolution of aquatic locomotor specializations and habitat transitions in tetrapod vertebrates. Integr. Comp. Biol. 56, 1310–1322 (2016).
Dickson, B. V. & Pierce, S. E. Functional performance of turtle humerus shape across an ecological adaptive landscape. Evolution 73, 1265–1277. https://doi.org/10.1111/evo.13747 (2019).
Pritchard, P. C. H. Encyclopedia of Turtles (TFH Publications, 1979).
Ernst, C. & Barbour, R. Turtles of the World (Smithsonian Institution Press, 1989).
Baillie, J. E. M., Hilton-Taylor, C. & Stuart, S. N. 2004 IUCN Red List of Threatened Species. A Global Assessment Vol. XXIV 191 (IUCN, 2004).
Ernst, C. H., Altenburg, R. G. M. & Barbour, R. W. Turtles of the World. (ETI BioInformatics, University of Amsterdam, 2006).
Jannello, J. M., Cerda, I. A. & de la Fuente, M. S. The relationships between bone shell microanatomy and palaeoecology in Testudinata from South America. Palaeogeogr. Palaeoclimatol. Palaeoecol. 537, 109412. https://doi.org/10.1016/j.palaeo.2019.109412 (2020).
Scheyer, T. M. & Sánchez-Villagra, M. R. Carapace bone histology in the giant pleurodiran turtle Stupendemys geographicus: Phylogeny and function. Acta Palaeontol. Pol. 52, 137–154 (2007).
Rieppel, O. & Reisz, R. R. The origin and early evolution of turtles. Annu. Rev. Ecol. Syst. 30, 1–22 (1999).
Rabi, M. & Sebők, N. A revised Eurogondwana model: Late Cretaceous notosuchian crocodyliforms and other vertebrate taxa suggest the retention of episodic faunal links between Europe and Gondwana duringmost of the Cretaceous. Gondwana Res. 28, 1197–1211. https://doi.org/10.1016/j.gr.2014.09.015 (2015).
Schwimmer, D. R. Late Cretaceous dinosaurs in eastern USA: A taphonomic and biogeographic model of occurrences. Dinofest International Proceedings, 203–211 (1997).
Rabi, M., Tong, H. & Botfalvai, G. A new species of the side-necked turtle Foxemys (Pelomedusoides: Bothremydidae) from the Late Cretaceous of Hungary and the historical biogeography of the Bothremydini. Geol. Mag. 149, 662–674. https://doi.org/10.1017/S0016756811000756 (2012).
Ferreira, G. S., Bronzati, M., Langer, M. C. & Sterli, J. Phylogeny, biogeography and diversification patterns of side-necked turtles (Testudines: Pleurodira). R. Soc. Open Sci. 5, 171773. https://doi.org/10.1098/rsos.171773 (2018).
de la Fuente, M. S., Sterli, J. & Maniel, I. Origin, Evolution and Biogeographic History of South American Turtles (Springer International Publishing, 2014).
Pereira, A. G., Sterli, J., Moreira, F. R. R. & Schrago, C. G. Multilocus phylogeny and statistical biogeography clarify the evolutionary history of major lineages of turtles. Mol. Phylogenet. Evol. 113, 59–66. https://doi.org/10.1016/j.ympev.2017.05.008 (2017).
Carranza, S. & Arnold, E. N. Investigating the origin of transoceanic distributions: mtDNA shows Mabuya lizards (Reptilia, Scincidae) crossed the Atlantic twice. Syst. Biodivers. 1, 275–282. https://doi.org/10.1017/s1477200003001099 (2003).
Pyron, R. A. Biogeographic analysis reveals ancient continental vicariance and recent oceanic dispersal in amphibians. Syst. Biol. 63, 779–797. https://doi.org/10.1093/sysbio/syu042 (2014).
Toussaint, E. F. A., Tänzler, R., Balke, M. & Riedel, A. Transoceanic origin of microendemic and flightless New Caledonian weevils. R. Soc. Open Sci. 4, 160546. https://doi.org/10.1098/rsos.160546 (2017).
Bower, D. S. et al. Salinity tolerances of two Australian freshwater turtles, Chelodina expansa and Emydura mawquarii (Testudinata: Chelidae). Conserv. Physiol. 4, cow042. https://doi.org/10.1093/conphys/cow042 (2016).
Adams, T. L., Polcyn, M. J., Mateus, O., Winkler, D. A. & Jacobs, L. L. First occurrence of the long-snouted crocodyliform Terminonaris (Pholidosauridae) from the Woodbine Formation (Cenomanian) of Texas. J. Vertebr. Paleontol. 31, 712–716. https://doi.org/10.1080/02724634.2011.572938 (2011).
Main, D. J. Appalachian delta plain paleoecology of the Cretaceous Woodbine Formation at the Arlington Archosaur Site, North Texas PhD thesis, The University of Texas (2013).
López-Conde, O. A., Pérez-García, A., Chavarría-Arellano, M. L. & Alvarado-Ortega, J. A new bothremydid turtle (Pleurodira) from the Olmos Formation (upper Campanian) of Coahuila, Mexico. Cretaceous Res. https://doi.org/10.1016/j.cretres.2020.104710 (2020).
Scotese, C. R. Atlas of Late Cretaceous Maps, PALEOMAP Atlas for ArcGIS, Volume 2, The Cretaceous, Maps 16–22, Mollweide Projection, PALEOMAP Project, Evanston, IL (2014).
Rasband, W. S. ImageJ. (U. S. National Institutes of Health, Bethesda, MD, USA, 1997–2016).
Goloboff, P. A., Farris, J. S. & Nixon, K. C. TNT, a free program for phylogenetic analysis. Cladistics 24, 774–786. https://doi.org/10.1111/j.1096-0031.2008.00217.x (2008).
Joyce, W. G. Phylogenetic relationships of Mesozoic turtles. Bull. Peabody Museum Nat. History 48, 1–102. https://doi.org/10.3374/0079-032X(2007)48[3:PROMT]2.0.CO;2 (2007).
Joyce, W. G. et al. A nomenclature for fossil and living turtles using phylogenetically defined clade names. Swiss J. Palaeontol. 140, 1–45. https://doi.org/10.1186/s13358-020-00211-x (2021).
Laurin, M., de Queiroz, K., Cantino, P., Cellinese, N. & Olmstead, R. The PhyloCode, type, ranks, and monophyly: A response to Pickett. Cladistics 21, 605–607. https://doi.org/10.1111/j.1096-0031.2005.00090.x (2005).
Lee, A. H. & Simons, E. L. R. Wing bone laminarity is not an adaptation for torsional resistance in bats. PeerJ 5(e823), 1–26. https://doi.org/10.7717/peerj.823 (2015).
Lee, A. H. & O’Connor, P. M. Bone histology confirms determinate growth and small body size in the noasaurid theropod Masiakasaurus knopfleri. J. Vertebr. Paleontol. 33, 865–876 (2013).
This research was supported by Midwestern University Intramural Funds (AG, HFS). The authors thank the Perot Museum of Nature and Science, and the numerous staff, students and volunteers who have collected and processed fossil material at the AAS. We especially thank A. Fiorillo, R. Tykoski, and K. Morton. We also thank Avery A. Williams for histological sampling of fossil material in this study, and Andrew Lee and Kelly Ezell for histological training and consultation. Drs. W.G. Joyce and G.S. Ferreira provided helpful feedback and guidance that greatly improved the quality of the manuscript.
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Adrian, B., Smith, H.F., Noto, C.R. et al. An early bothremydid from the Arlington Archosaur Site of Texas. Sci Rep 11, 9555 (2021). https://doi.org/10.1038/s41598-021-88905-1