miR-138 reduces tumorigenicity of GBM cells in orthotopic in vivo model. (A) Mouse survival was analyzed by Kaplan–Meier survival curve. Intracranial xenograft tumor was induced by implanting GBM cells transduced with miR-138 or miR-Ctrl expressing lentiviruses. (B) The expression levels of CD44/p27 axis were analyzed by western blotting on the harvested mice brain tumor tissues that were derived from GBM cells (GBM28 or GBM43) with miR-138 or miR-Ctrl expression. (C) Representative images of tissue sections from mice brain tumor tissues after immunohistochemistry staining with anti-CD44 or anti-p27 for the change of target gene expressions by miR-138, and Ki76 for cell proliferation. Scale bars indicate 100 µm.