Study on effects of sweating treatment and different drying methods on the quality of Gentianae macrophyllae Radix

To provide basis for improving the quality of Gentianae macrophyllae Radix and optimising the field processing method. Gentianae macrophyllae Radix samples were collected from Long County. Five main active iridoids were determined by using HPLC. The HPLC fingerprints were measured and analysed by the traditional Chinese medicine chromatographic fingerprint similarity evaluation system. Water, total ash, acid insoluble ash and ethanol extract contents were determined in accordance with Chinese Pharmacopoeia (2015). Colour of the surface and powdered samples were determined by using a colorimeter. Data were analysed by SPSS11.0. Notable differences were found among samples from different sweating and drying processes, in spite of the relatively consistent overall chemical characteristics. Sweating and drying methods influenced the contents of some active ingredients and colour very significantly; moreover, they also showed significant effects on the water, ash, and ethanol extract contents. The necessity of sweating treatment in the field processing of Gentianae macrophyllae Radix may be insufficient. Drying in the shade and oven drying are more profitable for preserving the active constituents. Gentianae macrophyllae Radix should be dried directly without sweating, and drying in the shade and oven drying should be adopted preferentially to improve the quality.

of the herb, making it looks like sweat 13 , and some of the chemicals in the herb change, making it change colour and smell. In the process, the change of temperature and water will change the activity of many enzymes, and then affect the transformation reaction of metabolites, leading to changes in the quality and properties of medicinal materials 14 . Proper drying methods, as well as sweating treatment, will have important effects on the quality of medicinal herbs 15 . Early studies revealed that the retention amount of gentiopicroside in Gentianae macrophyllae Radix which were dried after being cleaned while fresh first would be higher than that of Gentianae macrophyllae Radix, which were dried first before cleaning 16 . When dried at 100 °C, the loss of gentiopicroside is reduced to the minimum 17,18 . However, the effects of drying methods for gentiopicroside and other iridoids in Gentianae macrophyllae Radix have not been investigated. Only a few studies have investigated the sweating treatment of Chinese medicinal herbs, which mainly focused on Magnoliae officinalis cortex and Ephedrae herba. Studies on the history of field processing of Gentianae macrophyllae Radix show that sweating treatment of this medicinal herba appeared very late in modern times 19 . Until now, no report exists regarding the effect of sweating treatment on the quality of this crude drug. Moreover, the Chinese Pharmacopoeia 1 continues to adopt two kinds of processing methods, namely, sweating and without sweating. In this paper, field processing of Gentianae macrophyllae Radix with sweating treatment and direct drying without sweating, as well as different drying methods, were carried out. The contents of five main active components and the contents of water, total ash, acid-insoluble ash and ethanol extract were determined and combined with fingerprint analysis and colour analysis to evaluate the quality of samples from different processing methods. Exploring the effects of sweating treatment and different drying methods on the quality of Gentianae macrophyllae Radix can favor the optimization of drying methods in field processing, and provide basis for improving the quality of this important crude drug. Ltd. The standard of gentiopicroside was isolated in our laboratory and identified by using nuclear magnetic resonance spectroscopy, with a purity of more than 98%. Moreover, super pure water was purified by Milli-Q system (Millipore, Bedford, MA, USA) and filtered through a 0.22 µm filter. HPLC grade acetonitrile (TEDIA) and other reagents were of AR grade (Xi'an Chemical Reagent Factory).

Materials and reagents. Gentianae macrophyllae
Methods. Preliminary processing of medicinal material herb. After excavation, non-medicinal parts were removed, leaving only the roots. Dirt on the roots was carefully brushed off with a trifle moist brush. The sample material herbs were evenly divided into seven portions, and three portions were classified into group A (sweating treatment) and three portions were classified into group B (direct drying without sweating treatment). Three kinds of drying methods (drying in shade, drying in the sun and oven drying) would be applied to dry each portion of the two groups (A and B). The remaining portion was cut into slices (2-3 mm) for quick oven drying.
Sweating treatment. Roots of Group A were softened by basking, and then stacked in stacks of 28 cm-22 cm-7 cm in length-width-height, covered with a woven bag, and placed at room temperature for sweating treatment. The condition of sweating was monitored closely. Sweating treatment was stopped when the roots were soft and the surface was dry.
Drying methods. Three methods were adopted for drying the samples Oven drying: drying in an oven at 100 °C, the model of the oven is constant temperature air-blast dryer (Shanghai Lang Gan Experimental Equipment Co., Ltd., DHG-9123A). Drying in the sun: samples were exposed in sunlight at an average temperature of 22 °C. Drying in shade: samples were placed in a cool, ventilated place to dry. When the weight of the medicinal material was constant and it was easy to be broken off, the drying was completed. Processes of samples from group A and B corresponded to the same drying method to maintain the same drying time.
Determination of main active constituents. Apparatus and chromatograph condition. Agilent 1200 high performance liquid chromatography (Agilent), photodiode array detector, automatic injector and column temperature box.
The chromatographic conditions based on the method in the literature 20 were slightly modified. AE.LICHROM-C 18  To assess the precision of the method, the injected standard solutions were used. The intra-and inter-day coefficients of variation were both less than 5.0% (n = 5), which were satisfactory. The stability and recovery were evaluated according to the Chinese Pharmacopoeia 1 .
Chromatograms at 214, 238, 254 and 272 nm were measured. At the wavelength of 238 nm, five iridoid glucosides showed relatively large absorption, and their quantitative accuracy values were higher; therefore, 238 nm was chosen as the detection wavelength for determination. Fingerprint analysis. The fingerprint analysis was carried out according to the method in the literature 11 . Typical fingerprints are shown in Fig. 2.
Determination of water, ash and ethanol extract. The moisture content was measured by loss on drying method, using glass evaporating dish (100 mm, Beijing Glass Co., Ltd). Ash content was determined by residue on ignition method and muffle furnace (2001 model, TDW series temperature controller, Wenzhou Shuangyu Instrument Factory) was used. The ethanol soluble substance was extracted by Soxhlet extraction, using Soxhlet extractor (250 mL, Tianjin Glass Instrument Factory). These determinations were carried out following the standards for Gentianae macrophyllae Radix in Chinese Pharmacopoeia 1 : the second method of general rule 0832 for water; the general rule 2302 for total ash and acid-insoluble ash; the hot-maceration method of the general rule 2201 for ethanol soluble extract.
Colour analysis. Apparatus and determination condition. NS800 colorimeter (Shenzhen 3nh Technology Co., Ltd.). Light source D65, LED blue light excitation; measurement geometry 8°/day; silicon photodiode detector; measurement aperture 4 mm; location mode: lighting location and cross positioning. Colour space CIE, L*a*b*, among them, L* is the brightness of the colour, L* = 0 is black, L* = 100 is white; a* is between red and green, a* negative value is green, positive value is red; b* is between yellow and blue, b* negative value is blue, positive value is yellow. www.nature.com/scientificreports/ Colour determination of surface and powder. Both surface colour of Gentianae macrophyllae Radix and powder were determined. A total of three roots were randomly selected for each sample for determination. Because the bark colour of same root may vary, to ensure accuracy and consistency, the colours of the three parts, namely, the top part, medium part and lower part, of one same root were determined; moreover, the measurement of each part was repeated thrice, with the RSD < 3%. The powder was uniform in colour; thus, each sample was determined thrice.
Data analysis. All the data were input into EXCEL software and analysed by SPSS11.0. The traditional Chinese medicine chromatographic fingerprint similarity evaluation system version 2.0 (National Pharmacopoeia Committee 2012) was adopted in the fingerprint analysis.
Statement. The plant materials used in this experiment were cultivated Gentiana macrophylla, purchased from Badu Township, Longxian County. Local planting of Gentiana macrophylla complies with PRC and local laws and regulations. The experimental material was planted Gentiana macrophylla, which is not a protected plant. It is a cash crop planted in a traditional cultivation method and does not require specific permission for harvest.

Results
Results of sweating treatment and drying. Sweating treatment of Gentianae macrophyllae Radix lasted two weeks. During the course, the temperature in the crude drug stacks increased. The medicinal material shrank, the surface felt moist and the colours darkened. The surface colour of the medicinal material that was dried after sweating was darker than that of the directly dried samples without sweating. Different drying methods took different durations. Slice oven drying was the fastest drying method and took about 3.5 h. The time of slice drying was used as a reference to determine the drying time of other drying methods. Experimental results showed that oven drying had the shortest drying time among the three methods and can be completed in a few hours. Drying in the shade took the longest time of 2 weeks. Sun-drying took the median amount of time. Under the conditions of our experiment, with the average daily sun of about 7 h, drying Gentianae macrophyllae Radix can be finished in a week. The specific drying time is shown in Table 1.   Table 2. Double-factor variance analysis of the sweating treatment and drying method showed that the loganic acid (I1) in the samples that were dried in the sun and dried in the shade after sweating treatment were significantly lower than those in the other samples. Moreover, the content of 6-O-β-glucosylgentiopicroside (I2) in the oven-dried samples without sweating and directly slice-dried samples were significantly lower than those in the other samples. Additionally, the content of gentiopicroside (I4) in the sample dried in the shade directly without sweating was significantly higher than those in the other samples. No significant difference in the contents of swertiamarin (I3) and sweroside (I5) were found in the samples. Furthermore, the sum of the contents of the five active constituents in the samples dried in the shade without sweating was significantly higher than those in the other samples; whereas, the sum was significantly lower in the sun-dried samples with sweating.
The results of ANOVA (Table 3) showed that sweating treatment had a significant influence on the contents of I1, I2 and I4. The contents of I1 and I4 and the sum of the contents of the five active constituents in the samples with sweating treatment were significantly lower than the direct drying samples, whereas I2 in the samples with sweating treatment was significantly higher than the direct drying samples. The contents of I3 and I5 did not differ significantly between the sweating and non-sweating samples.
The results of ANOVA for the different drying methods are shown in Table 4. The content of I4 and the sum of the five active constituents in the samples dried in shade were extremely significantly higher than in the samples from other drying methods, I3 was significantly higher than in the samples from other drying methods. In oven-dried samples, I1 was significantly higher than those in the other samples, whereas I2 was significantly lower. The contents of I1 and I4 and the sum of the five active constituents in the sun-dried samples were lowest among all samples.

Factor I1 (%) I2 (%) I3 (%) I4 (%) I5 (%) Sum of the five iridoids (%)
Oven www.nature.com/scientificreports/ peaks. These 15 peaks can fully represent the whole chromatograph, because their total area accounted for 99% of the total fingerprint area. The similarity calculation showed that the similarity of all samples was above 0.999, indicating that the holistic chemical characteristics of each sample with different sweating treatments and drying were relatively alike and only slightly differentiated.

Results of determination of water, ash and ethanol extract.
The results of determination of water, ash and ethanol extract contents are shown in Table 5. All samples reached the prescribed standard of Gentianae macrophyllae Radix in Chinese Pharmacopoeia (2015 edition). Two-factor analysis of variance showed no significant difference among the water, ash and ethanol extract contents of each sample. Single factor analysis ( Table 6) showed that sweating treatment had a significant effect on ethanol extract content. The ethanol extract content in the non-sweating samples was higher than that in the sweating samples. The differences among water, total ash and acid-insoluble ash contents were not significant.
The results of ANOVA for the different drying methods are shown in Table 7. Water content in the oven-dried samples was significantly lower than those in the sun-dried and dried in the shade. Total ash of the sun-dried samples was significantly higher than those of the oven-dried and dried in shade. No significant differences were found in the acid-insoluble ash and ethanol extract contents among the three drying methods. Fig. 3, distinct colour differences among samples from different processing methods can be seen by naked eye, either at the surface colour or drug powder colour. The results of colour determination are shown in Table 8. The results of ANOVA according to sweating treatment or non-sweating treatment and different drying methods are shown in Tables 9 and 10, respectively. The L* and b* values of surface colour of the samples from sweating treatment were extremely significantly lower, and the a* value of the sweating sampled was significantly lower than the non-sweating samples. Meanwhile, the L* value of powder colour of samples with sweating treatment was significantly lower than the non-sweating samples. This revealed that sweating can notably deepen surface colour. However, Table 10 revealed that only the a* value of surface colour of the sun-dried sample was significantly higher than those of samples from other drying methods. Moreover, a* and b* values of powder colour of the oven-dried samples were significantly higher than those in the samples from shade-dried and sun-dried methods. Table 5. Determination of water, total ash, acid-insoluble ash and ethanol extracts (M ± S, n = 3).

Discussion
Effect of sweating treatment. The gentiopicroside and loganic acid are important contents of Gentianae macrophyllae Radix quality evaluation in Chinese Pharmacopoeia. Therefore, it is an important aspect to investigate the influence of sweating treatment on the main components of iridoid glycosides. In this paper, the results showed that the contents of main active iridoid glycosides in Gentianae macrophyllae Radix treated by sweating and that treated without sweating were significantly different. The contents of I1 and I4, the two index components in Chinese Pharmacopoeia, in non-sweating herbs were significantly higher than those in the sweating herbs. The 6-O-β-glucosyl-gentiopicroside (I2) in the sweat samples was significantly higher than that in the non-sweat samples, but its content was relatively low, which had little effect on the overall quality condition. The above results are consistent with the previous research results 21,22 . The content of ethanol extract of Gentianae macrophyllae Radix with sweating treatment was significantly lower than without sweating treatment. This may be due to the fact that during sweating, the cells of root tissue remain breathing for a period of time, consuming more substances than the samples dried directly without sweating, resulting in a decrease in extract content.

Effect of different drying methods.
Studies have shown that the drying method will affect the content of medicinal constituents. Some components of medicinal materials in the drying process will increase and some may decompose. These effects would be highly remarkable for crude drugs in which the main components are compounds with active chemical properties. When drying Lonicerae japonicae flos at low temperature, the content of secoxyloganin, an iridoid glycoside, increases, whereas the content of morroniside tends to reduce 23 . Under different drying conditions, the trends of composition changes differ. The retention of each component is different, and results in the difference of the internal quality of medicinal materials. Similar to secoxyloganin and morroniside in Lonicerae japonicae flos, the main effective components of Gentianae macrophyllae Radix, such as gentiopicrside and loganin acid, are iridoid glycosides with active chemical properties. These would be affected by drying methods in all probability during field processing. Based on comprehensively analysis of the contents of five main components, drying in shade was the most conducive method for preserving the effective components, in spite of I1 content being lower. The total contents of main constituents in oven-dried samples were lower than those in shade-dried samples; however, oven drying had the advantage of faster drying time.
The oven-drying method had two advantages to drying in the sun: the contents of the two index components (I1 and I4) in the pharmacopoeia were high; the time consumption was short. Therefore, drying in shade was the best method, oven drying was the second, and drying in the sun was the least effective for retaining the active components. This may be because prolonged heat during drying in the sun destroys the active ingredients 22 . The water contents of all samples from three drying methods were all less than 8%, reaching the prescribed standard of China Pharmacopoeia (2015 edition). Water content in the oven-dried sample was significantly lower than those in the other samples. Moreover, the total ash and acid-insoluble ash contents of all samples reached the prescribed standard of China Pharmacopoeia. Among them, the acid-ash content of the sample dried in shade was highest among all samples, which may be due to the fact that the samples were easily polluted by environmental dust during the long drying in the shade. Therefore, the environment must be kept clean when herbs are being dried in the shade.

Effect of sweating treatment and drying methods on colour.
Colour is an important aspect of quality evaluation of traditional Chinese medicinal materials. Traditionally, the faint yellow variety of Gentianae macrophyllae Radix is the best 24 . This means the lighter colour represents relatively good quality. Sweating treatment significantly affected the colour of Gentianae macrophyllae Radix, especially the surface colour, and can notably darken the colour of medicinal materials. This is consistent with the research results in the literature 21 .
The reason for this phenomenon may be the activation of some enzymes during sweating, which causes the hydrolysis and repolymerization of some iridoid glycosides to form dark precipitation. Therefore, sweating treatment is not good for the appearance of Gentianae macrophyllae Radix. The effect of drying method on colour is less apparent than that of sweating; however, drying in the sun can redden the surface of the medicinal materials, and oven drying can make the colour of the medicinal powder more red and yellow.

Conclusion
Fingerprint analysis showed that the overall chemical characteristics of Gentianae macrophyllae Radix in different treatments were relatively consistent, and sweating treatment and drying methods would not essentially differentiate the overall quality of this crude drug. However, some remarkable differences were found in the quality of dried herbs under different sweating and drying conditions. In terms of the contents of active constituents, www.nature.com/scientificreports/ non-sweating Gentianae macrophyllae Radix was superior to those that underwent sweating treatment. In addition, no record of sweating treatment of Gentianae macrophyllae Radix exists in ancient Materia Medica. Moreover, sweating can significantly darken the surface colour, reducing the appearance quality. And sweating treatment often takes weeks longer as well as it only appeared in the field processing of Gentianae macrophyllae Radix very late in modern time. Therefore, the basis for sweating treatment of Gentianae macrophyllae Radix may be insufficient. Among these three ways of drying: oven drying, drying in the sun and drying in shade, drying in shade and oven drying were more conducive to preserving the active constituents. The quality of medicinal materials dried by these two methods was better. In summary, in the field processing of Gentianae macrophyllae Radix, herbs should be dried directly and without sweating, and drying in the shade or oven drying should be adopted preferentially to improve the quality.