Figure 5 | Scientific Reports

Figure 5

From: Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels

Figure 5

PDGFRa+ cells generate SMCs after wire injury. (A) Schematic outlining experimental strategy. (B) Hematoxylin eosin-stained carotid artery at 14 days after wire injury. The dotted box area is shown in the inset present an image with higher magnification. Scale bar: 100 µm. (C) Fluorescence image showing the distribution of tdTomato+ cells at 14 days after wire injury. Scale bar: 100 µm. (D) Quantification of the percentage of tdTomato+ cells in the media before and after wire injury. Data are presented as the mean ± SEM, day 0, N = 3, 14 days after wire injury, N = 4. Unpaired t-test, n.s.: not significant. (E) Quantification of the number of tdTomato+ cells in the media before and after wire injury. Data are presented as the mean ± SEM, day 0, N = 3, 14 days after wire injury, N = 4. Unpaired t-test, n.s.: not significant. (FM) Immunostaining for Sca1 (F), αSMA (G), SM-MHC (HI), CD31 (J), VE-cad (K), CD45 (L), and CD68 (M) on carotid artery sections at 14 days after wire injury. Arrows indicate tdTomato+ /Sca1+ cells in the media. L: lumen, I: intima, NI: neointima, M: media, A: adventitia. Scale bars: 20 µm. See also Figure S5.

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