Figure 4 | Scientific Reports

Figure 4

From: Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels

Figure 4

Two types of neointima formed by distinct SMCs after carotid artery ligation. (AB) Representative images of type I (A) and type II (B) neointima at 56 days after ligation. Scale bars: 100 µm. (CD) Immunostaining for Sca1 on carotid artery sections from type I (C) and type II (D) neointima. The dotted boxed area is shown on the right with higher magnification. Quantification of the percentage of Sca1 expressing cells in tdTomato+ cells within the vessel wall. Data are presented as the mean ± SEM, N = 3–8 per group. NI: neointima, M: media, A: adventitia. Scale bars: 50 µm. Scale bars in insets: 20 µm. (EF) Immunostaining for αSMA on carotid artery sections from type I (E) and type II (F) neointima. Arrowheads indicate tdTomato+/αSMA+ cells in the neointima. Arrows indicate tdTomato+/αSMA- cells. The dotted box area is shown on the right present an image with higher magnification. Quantification of the percentage of αSMA-expressing cells in tdTomato+ cells within the vessel wall. Data are presented as the mean ± SEM, N = 3–8 per group. NI: neointima, M: media, A: adventitia. Scale bars: 100 µm, Scale bars in insets: 20 µm. (GH) Immunostaining for SM-MHC on carotid artery sections from type I (G) and type II (H) neointima. The dotted box area is shown on the right with higher magnification. NI: neointima, M: media, A: adventitia. Scale bars: 100 µm, box, Scale bars in insets: 20 µm.

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