Figure 3 | Scientific Reports

Figure 3

From: Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels

Figure 3

Contribution of PDGFRa+ cells to neointima formation following carotid artery ligation. (A) Schematic outlining the experimental strategy. (B) Hematoxylin eosin-stained carotid artery at 28 days after ligation. Scale bar: 100 µm. (C) Fluorescence image showing the distribution of tdTomato+ cells at 28 days after ligation. NI: neointima, M: media, A: adventitia. Scale bar: 100 µm. (DF) Immunostaining for Sca1 (D), αSMA (E), and SM-MHC (F) on carotid artery sections at 28 days after ligation. NI: neointima, M: media, A: adventitia, T: thrombus. Scale bars: 100 µm. (G) Hematoxylin eosin-stained carotid artery at 56 days after ligation. Scale bar: 100 µm. (H) Fluorescence image showing the contribution of tdTomato+ cells to neointima at 56 days after ligation. NI: neointima, M: media, A: adventitia. Scale bar: 100 µm. (I) Quantification of the contribution of tdTomato+ cells to neointima formation. Data are presented as the mean ± SEM, 28 days, N = 10, 56 days, N = 11, **p < 0.01, chi-square test. See also Figure S4.

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