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Continuous presence of proto-cereals in Anatolia since 2.3 Ma, and their possible co-evolution with large herbivores and hominins

Abstract

Cereals are a central resource for the human diet and are traditionally assumed to have evolved from wild grasses at the onset of the Neolithic under the pressure of agriculture. Here we demonstrate that cereals may have a significantly longer and more diverse lineage, based on the study of a 0–2.3 Ma, 601 m long sedimentary core from Lake Acıgöl (South-West Anatolia). Pollen characteristic of cereals is abundant throughout the sedimentary sequence. The presence of large lakes within this arid bioclimatic zone led to the concentration of large herbivore herds, as indicated by the continuous occurrence of coprophilous fungi spores in the record. Our hypothesis is that the effects of overgrazing on soils and herbaceous stratum, during this long period, led to genetic modifications of the Poaceae taxa and to the appearance of proto-cereals. The simultaneous presence of hominins is attested as early as about 1.4 Ma in the lake vicinity, and 1.8 Ma in Georgia and Levant. These ancient hominins probably benefited from the availability of these proto-cereals, rich in nutrients, as well as various other edible plants, opening the way, in this region of the Middle East, to a process of domestication, which reached its full development during the Neolithic.

Introduction

An interdisciplinary study was carried out on the lacustrine sedimentary sequence of Acıgöl (Lakes district, SW Turkey, (Fig. 1). Dated from 0 to 2.3 Ma1 and extending over 601 m in length, it covers almost the entire Quaternary. With rather constant sedimentation rates, ranging from 21 to 35 cm ka1, Lake Acıgöl provides an exceptional palaeoecological archive, and is the longest lacustrine record for western Asia. Age control (Fig. 2) was provided by the paleomagnetic identification of the Bruhnes-Matuyama Boundary, the Jaramillo and Olduvai subchrons, as well as cyclostratigraphy1. The glacial/interglacial pseudo-cycles at around 100 ka period were recorded in magnetic susceptibility as well as major elements composition (see methods and1). The oldest Homo erectus remains from Turkey (the Kocabaş skull) were discovered in the travertine deposit of Denizli 40 km W of Acıgöl lake2,3 and dated to ca 1.2–1.6 Ma4, documenting one of the main early migratory axis of hominin populations between Africa and Eurasia. Simultaneous or later signs of early Pleistocene human occupation based on lithic tools were found in Anatolia, in particular in the nearby Gediz river site5 (120 km NW of Acıgöl) and Dursunlu site (180 km E of Acιgöl). More distant earlier presence of hominins around ~ 1.8 Ma were evidenced in the Levant6, Georgia7 and Ciscaucasia8, pointing toward the high probability of hominin occupation of Anatolia, mid-way of Levant and Caucasus, at least sporadic since 1.8 Ma. Evidence from China and Jordan points toward even earlier presence of hominins out of Africa as early as 2.19 and 2.5 Ma10, respectively. In the Denizli and Burdur basins, adjacent to Acıgöl, abundant fossil remains of large mammals typical of the late Villafranchian have been unearthed, including an extinct species of primitive mammoth (Archidiskodon m. meridionalis), an extinct rhinoceros (Stephanorhinus cf. etruscus), several species of horses (Equus) sp., E. cf. altidens/E. cf. mygdoniensis and E. cf. apolloniensis), small and large-sized deer (Metacervoceros rhenanus, Arvernoceros sp., Cervalces (Libralces) ex gr. minor-gallicus), a large primitive okapi (Palaeotragus sp.), a primitive camel (Paracamelus cf. gigas), a large bovine today extinct (Leptobos cf. etruscus) and several antelopes (Gazellospira torticornis, Gazella sp.) 11,12,13,14. They indicate that Homo erectus coexisted with a rich and diversified mega-fauna from which they were largely dependent15.

Figure 1
figure1

Simplified geological map and location of the sites. Redrawn from Alçiçek et al.16

Figure 2
figure2

Time/depth curve of Acıgöl core, with paleomagnetic tie point in black, normal geomagnetic polarities in gray. Earliest hominin presence in Anatolia [4], Ciscaucasia [8], China plus Jordan [9, 10], is indicated in red, blue and green, respectively.

Results

Vegetation history of the Acıgöl area

Our palynological analyses of 72 regularly spaced samples show a diversified vegetal landscape alternately wooded and open, in response to orbitally driven climatic cyclicity. However, arboreal pollen values remain almost constantly below 50% of the Pollen Sum (PS) (average 27.5%, median 22.8%), which corresponds to an overall open landscape (Fig. 3). Among herbaceous plants, the dominant taxa are steppics such as Artemisia, heliophilous and halophilous taxa including Calystegia, several Compositae, Convolvulus, Linum, Plantago ssp., Poaceae and Chenopodiaceae that could develop on the saline shores of Acıgöl lake during evaporitic periods. Forests are composed of a mixture of conifers, Mediterranean Pinus, Abies, Cedrus, Cupressaceae and Picea, associated with broadleaved trees dominated by Mediterranean oaks, i.e. deciduous and evergreen Quercus, with some Olea. Riverine trees such as Alnus, Salix, Populus, Tamarix, Juglans and Platanus have also been identified. Few Tertiary or megathermic relictual taxa (Carya, Liquidambar, Parrotia, Pterocarya fraxinifolia, Taxodiaceae, Tsuga, Zelkova) were identified so far in the pollen assemblages, mostly before 2.2 Ma, due to climatic cooling17,18 since the end of Tertiary which led to a decline in global biodiversity19,20.

Figure 3
figure3

Simplified pollen and NPP diagram in percentages of Acıgöl, core 3, based on the age model of Demory et al. [1]. Equidistant scale. Values are in percentages calculated on a pollen sum without Non-Pollen Palynomorphs (NPP), Ferns, Bryophytes and Algae. The beige rectangle corresponds to the date of the presence of Homo erectus at Kocabaş (Lebatard et al. [4]).

The vast freshwater stretch of Acıgöl, located in a predominantly arid limestone hills environment, seems to have been a crucial resource for the mammalian fauna, which probably concentrated around the site in search of water and pastures. Indeed, low percentages of arboreal pollen imply that the landscape remained open throughout the sequence and suggest a marked grazing pressure by herbivores in addition to climatic factors21,22,23.

Coprophilous fungi spores, cereals and other ancestors of cultivated plants

Coprophilous fungi spores are excellent indicators of herbivorous mega-mammal herds since they grow exclusively on dung deposited by these animals24. At Acıgöl, a wide variety of coprophilous fungi spores has been identified throughout the pollen record including: Sporormiella sp., Podospora sp., Delitschia sp., Sordaria sp. and Valsaria variospora (Figs. 3, 4). They provide evidence for a continuous presence of large herbivorous mammals around the lake throughout Quaternary.

Figure 4
figure4

Coprophilous fungi spores of Acıgöl, core 3. Equidistant scale. Age model is from Demory et al. [1]. In red: coprophilous fungi taxa..

Pollens of Poaceae, such as Secale (rye) and Cerealia-type, have been identified throughout the sequence (Figs. 3, 5). Unexpectedly, they present the same morphological characteristics as that of modern cereal grains25,26, namely an average size of ≥ 40 µm and a large pore + annulus (≥ 8 µm). As by definition cereals are cultivated plants, we will call the corresponding plants “proto-cereals” to highlight that their pollen are identical to those of cereals. This resemblance can be seen clearly in Fig. 5, where we have brought together fossil cereals from Acιgöl (Fig. 5, photos 1–7), from Roman time (Fig. 5, photo 8), not modified by modern agricultural practices, and from the current wheat field of the Lauragais agricultural plain, Gardouch, France (Fig. 5, photo 9). Cerealia-type frequencies reach a maximum of 9% of the PS around 2.2 Ma and can be as abundant as wild Poaceae pollen (Fig. 3). The Cerealia/Poaceae ratio shows that 24.66% of all Poaceae are proto-cereals from 2.0 to 2.3 Ma (Supplementary Table 1). Such high proto-cereal rates are almost never reached in pollen records, even in recent periods and in the presence of agriculture, because of the very low pollen dispersal capacity of cereals27. A lowering of frequencies down to 2–4% range is recorded in younger periods (Fig. 3), as well as a step like decrease of the Cerealia/Poaceae ratio (Fig. 6). This change may be related to the Middle-Pleistocene Transition (MPT) cooling and to the mega-mammal fauna change from a Villafranchian to a Galerian type28. MPT and faunal changes occurred around 0.9–1.0 Ma, while a decrease in our proto-cereal starts around 1.5 Ma, however signs of cooling and amplified climatic cycles predate the MPT28.

Figure 5
figure5

Pollen grain of Cerealia and Triticum sp. from Acıgöl (ACI), core 3 (photos 1–7), the Roman site of La Verrerie, Arles, France (photo 8) and Gardouch, France, current wheat field (photo 9). Photographies with a photonic (photo 1 – 4 and 8) and a confocal microscope (photos 5-7 and 9). 1) sample ACI 239 m, age: 0.871 Ma. 2) sample ACI 435.50 m, age: 1.709 Ma. 3) sample ACI 532.44 m, age: 2.122 Ma. 4) sample ACI 509.50 m, age: 2.026 Ma. 5) sample ACI 552.57 m, age 2.206. 6) sample ACI 552.57 m, age: 2.206 Ma. 7) sample ACI 429.50 m, age: 1.681 Ma. 8) sample La Verrerie 1455, age: 50-70 BC (Roman). 9) current pollen of Triticum sp., age: 2000 AD. L: maximal length (µm).

Figure 6
figure6

Cerealia/Poaceae ratio in %, % cultivated tree ancestors and % Olea of Acıgöl, core 3.

The histogram of wild Poaceae and proto-cereal pollen size (Fig. 7a) shows that there are a number of pollen populations modes around 30, 37.5, 45–50, supporting the idea that the larger grain sizes cannot be interpreted as a tail of ‘anomalous’ wild Poaceae pollen. Moreover, comparison with the present-day pollen rain recorded in moss pollsters, sampled around the lake of Acıgöl (Fig. 7b and Supplementary Table 2), show that the large pollen size mode (≥ 40 µm) is nowadays nearly absent (0–0.97% of the PS, Cerealia/Poaceae ratio of 4.52%, Supplementary Tables 3 and 4), even in biotopes with wild Poaceae considered to be ancestors of cereals (Aegilops, sample 2a, cereal rate: 0.97% of the PS) or with cereals such as Hordeum (sample 3a, b and 4, cereal rate 0.31, 0.00, 0.33 of the PS respectively, Supplementary Tables 2 and 3).

Figure 7
figure7

a) Pollen size of wild Poaceae and proto-cereal of Acıgöl, core 3. The measurements were made on the 10 samples with the highest cereal pollen content. A total of 991 grains of pollen were measured. b) Current pollen rain at the Acıgöl lake and surroundings. 8 moss samples were collected and 354 measurements of the longest axis of the wild Poaceae and cereal pollen grain were made.

Our interpretation is that proto-cereals recorded throughout the Acıgöl sequence derive from wild Poaceae. Their emergence and predominance may have been favoured by the impact of large herbivore herds attracted to Acıgöl lake shores, and through genetic drift. Through the process of trampling, nitrogen enrichment of soils and browsing, large mammal herds could have altered the genotype of proto-cereals naturally present in Acıgöl and thus, favoured the emergence of modern cereals. For genetic reasons, the descendants of these proto-cereals are not represented today among cultivated Poaceae because domestication bottlenecks eliminate genetic variation29.

Is there a relationship between the size of proto-cereal pollen and climate? To our knowledge, the genetic literature does not show any relationship between the increase in pollen size and temperature. However, there does seem to be a relationship with atmospheric drought30,31 which is said to have favoured the appearance of polyploidy in certain species of Poaceae. It cannot be excluded that climate has had an influence on the proto-cereal genome, but only the interaction between herds of large herbivores and proto-cereal steppes can explain why proto-cereal pollen has never been found in such abundance elsewhere in Pleistocene pollen records.

The ancestors of cultivated trees (Olea sp., Juglans sp., Castanea sp., Corylus sp., Prunus t.), typical of the modern Mediterranean agriculture, are also present in the Acıgöl sequence (Fig. 3 and Supplementary Table 5). Their amount increases after 1.5 Ma, mainly due to Olea (Fig. 6). Other potentially edible plants such as Ephedra, Hippophae, all the Compositae and the Fagaceae have been identified in the pollen assemblages. They correspond to 54.4% of plants identified in the pollen assemblages. Among these plants, there are 72% grasses and 28% trees and, among edible organs, 51% are vegetables and 20% are seeds (Supplementary Fig. 1a,b). These results testify to the potential wealth of accessible food resources that human and animal populations could feed on. Interestingly, studies carried out in Spain on the present-day consumption of wild plants lead to results close to those obtained at Acıgöl, with 87% grasses and 13% trees32.

In recent years, new biological and archaeological data obtained from sites with human occupation have improved our knowledge of the beginnings of agriculture and the modalities of its implementation. In the Levant, the Ohalo II site highlights the presence of proto-cereal seeds, and flint tools to harvest, as early as 23,000 years before the present33. Further north, on the archaeological site of Gesher Benot Ya'aqov, proto-cereal seeds (oats, Avena) as well as pollen from cereals and trees currently cultivated, were identified over a period ranging from 750,000 to 820,000 years34,35. Moreover, recent genetic data indicate that the emergence of agriculture did not occur at a single location at the onset of the Neolithic (e.g. the "Fertile Crescent" hypothesis) but is, on the contrary, an evolutionary and multi-regional long-term phenomenon36,37,38. Alternatively, or simultaneously, are the hominins also partly responsible by having developed episodes of a form of transitory "proto-agriculture"? We already know that this domestication process was discontinuous with shutdown and restart phases37,39. Acheulean lithic tools, characterised by symmetrically shaped bifaces, testify to the rather advanced cognitive capacities of early Pleistocene populations that may have visited the lakeshore of Acıgöl5. Hominin populations may also have benefited from this opportunity to diversify their food regime with easily harvested and nutrient-rich wild plants (Supplementary Table 5), as it is the case today for hunter-gatherer populations in Africa and elsewhere in the world.

Discussion

Challenging the paradigm of domestication: how and when?

The question surrounding the Neolithic emergence with plant and animal domestication has been debated for decades and has been the subject of countless studies, in most cases carried out by archaeologists and geneticists focusing either on plant macrorests from archaeological sites or on the genome history of cultivated plants40,41. The study of natural environments (wetlands, lakes), such as at Acıgöl, and of their microbiological content has so far been largely neglected to tackle this question.

What happened in the Neolithic, when humans went from a hunter-gatherer to a farmer lifestyle? Did they reproduce conditions that existed two million years ago? Has there been a new stage of cereal speciation linked to humans? Vaughan42 emphasises that "the time scale of domestication of 10,000 years or less is a very short evolutionary time span" (p. 893). The proto-cereal pollen of Acıgöl appears to indicate that the genetic modification of cereals could have also been a natural process that appeared long before agriculture emerged, and that the conditions were already present when human populations shifted from hunter-gatherer to agricultural societies. Our results enable an important enigma to be reformulated in relation to human evolution: when did cereals appear and are humans solely responsible? Our study appears to challenge the long-held paradigm that humans were the progenitors of cereal grasses, when it seems in fact possible that cereal grasses may have appeared naturally, with humans simply accelerating their expansion. If this were confirmed by the presence of proto-cereal pollen in sediments of the lower Pleistocene or in older sediments from other regions, this would necessitate a fundamental revision of our overall vision of the history of human nutrition. To substantiate this, we can report our initial observation of similar proto-cereals in the Kocabaş travertine sequence (Fig. 8) at the early Homo erectus stage (1.2–1.6 Ma).

Figure 8
figure8

Simplified pollen and NPP diagram in percentages of Kocabaş (Faber quarry, 37°51’N, 29°21’E., 580 m alt.). 3 samples were taken in the fluviatile facies (lower and upper conglomerate) of the travertine formation (details in Lebatard et al. [4]).

Material and methods

Chronostratigraphy

188 oriented standard cylinders were regularly subsampled in Acıgöl core C3, from 100 to 600 m depth. Natural remanent magnetization (NRM) were measured with the Superconducting Rock Magnetometer (SRM 560R, 2G Enterprises) of the Rock Magnetic Laboratory in CEREGE. Demagnetization was mostly performed using alternating magnetic fields. Magnetostratigraphy1 allowed identifying the temporal tie points listed in Supplementary Table 6. Bruhnes age cyclostratigraphy based of Xray fluorescence analyses was proposed by Akcer-On43, leading to an estimated age of 700 ka at 170 m, coherent with the magnetostratigraphy. 566 samples were used to build a magnetic susceptibility curve versus depth, pointing toward a regular climatic cyclicity record throughout the core, in agreement with sedimentological and palynological proxies1.

Fossil pollen

Pollen analyses were conducted on 87 samples, of which 72 were polliniferous. Non-polliniferous samples correspond to micro-tephra layers (markers of volcanic eruptions or redeposition of detrital tephra) or levels rich in microcharcoals (fire indicators) or algae (blooms of Botryococcus or Chrysophyceae in response to a disturbance of the lacustrine trophic system). At Kocabaş, pollen analyses were conducted on three samples taken from fluviatile conglomerates, on both sides of the travertine deposit in which the remains of the Kocabaş Man were discovered and dated to 1.2–1.6 Ma4.

The sporo-pollinic material was extracted from the sediment using chemical treatment (HCl acid, NaOH 10% and acetolysis) followed by flotation in heavy liquid (d = 2) and 160 + 10 µm sievings44. The identification of pollen, spores and Non-Pollen Palynomorph (NPP) was carried out with a photonic microscope Biomed Leitz (500× magnification). Standard palynological identifications were based on the pollen reference collection of IMBE (CNRS, Aix-en-Provence, France), pollen photographic atlases45,46,47,48 and articles on NPP49,50,51. The pollen percentages were calculated on a Pollen Sum (PS) including all plants except Filicophytes, Bryophytes, Algae, and NPP. For each sample, pollen counting was carried out up to 300 to 400 grains (phanerogams alone) and then continued over the whole slide for rare taxa52. At Acıgöl, the mean PS varies from 3025 grains (all pollen, spores and NPP included) to 558 grains per sample (all pollen, without spores and NPP). The average pollen concentration (weighting method53) is of 7829 pollen/g sediment (minimum: 38, maximum: 451,556). The average weight of the samples is 11.24 g (minimum: 3.5 g, maximum: 16.6 g). The total number of taxa identified (pollen, spores, NPP, algae) is 201. At Kocabaş, the mean PS varies from 356 grains (all pollen, spores and NPP included) to 314 grains per sample (all pollen without spores and NPP). The average pollen concentration is 6.5 pollen/g sediment (minimum: 2.9, maximum: 9.9). The weight of the samples is 250 g each. The total number of taxa identified (pollen, spores, NPP, algae) is 69.

The identification of pollen of cereals relies on morphological characteristics25,53, especially the longest axis of the grain which is a reliable means to discriminate cereal grains, and the pore + annulus diameter which can be used as a complementary criterion. According to Andersen25, pollen of Poaceae with a longest diameter larger than 37 µm are pollen of cereals. For Bastin54 and Leroi-Gourhan55, the diameter of cereals should be ≥ 40 µm and the external diameter of the annulus should be at least 10 µm. Detailed information can be found in Emery-Barbier and Thiébault56. In our work, we have followed the proposal of Bastin54 and Leroi-Gourhan55 and designated as cereals the pollen of Poaceae with (1) a longest diameter ≥ 40 µm, (2) a large and protuberant annulus + pore, (3) and scabrate to verrucate surface sculpturing of the exine. We thus call proto-cereals (or Cerealia type) the Poaceae pollen ≥ 40 µm and wild Poaceae the others, although we acknowledge that some rare wild Poaceae may show diameter ≥ 40 µm, such as Aegylops sp., Glyceria sp.57. The measurements made on 991 pollen grain of wild Poaceae and proto-cereals of 10 samples of Acıgöl (core 3) chosen among the richest in pollen of cereals (Supplementary Table 1) show that the distribution is polymodal (Supplementary Fig. 1a). The mean pollen diameter of wild Poaceae and cereal is respectively 31.01 and 45.07 µm. The mean pore diameter is 4.28 µm (minimum: 2.5 µm; maximum: 5 µm) and the mean pore + annulus diameter is 10.12 µm (minimum: 6.25 µm; maximum: 13.75 µm). In the literature, the mean size of the cereal pore is 2.7 µm57. There is no relationship between the diameter of the wild Poaceae and proto-cereal and the size of the pore (R2 = 0.046) or the pore + annulus (R2 = 0.097).

Modern pollen rain

Sampling of moss pollsters were carried out in May 2017 along the Acıgöl lakeshores and surroundings, in a wide variety of vegetal landscapes, altitudes, and anthropisation levels (Supplementary Table 2), in order to reconstruct the modern pollen rain and to improve the accuracy of palaeoenvironmental reconstructions based on fossil pollen analyses. In this article, we show the results of the pollen analysis of  eight samples. The samples were taken on the lakeshores, in specific ecosystems with halophytic or unsalted vegetation. The objective was to evaluate the amount of cereals in the current pollen rain and to find out whether or not cereal pollen found in Acıgöl sediments may have originated from the hygrophilous vegetation of the lake shoreline. The results show that the pollen of cereal is underrepresented in the current pollen rain of Acıgöl (range of values between 0 and 0.97% of the PS, Cerealia/Poaceae ratio 4.52% against 16.9% (n = 72) in the Acıgöl core Supplementary Tables 3 and 4). This indicates that there is not a biological specificity among the Poaceae community of the Acıgöl lakeshore and that there is a good chance that the proto-cereal pollen of the Acıgöl sedimentary archives come from the surrounding steppe communities and not from hygrophilous wild Poaceae.

Data availability

All data generated or analysed during this study are included in this published article (and its Supplementary Information files) and are available on the e-site of EPD (European Pollen Database): http://www.europeanpollendatabase.net/data/downloads/image/ACIGOL3.zip.

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Acknowledgements

This research was conducted as part of the TUBITAK-CNRS bilateral cooperation with the grant number of 114Y723 during 2015–2017. The researches were further funded by ECCOREV (CNRS (ACIGÖL project), the LABEX OT-MED (Bilat project) and by the ANR (MRSEI, ACIGÖL project). The long coring of Acıgöl (core 3) was funded by ALKİM  Company due to the Acıgöl Drilling Project signed between Cahit Helvacı (DEU) and the ALKİM and  SODAS Companies. We are grateful to the companies of ALKİM  and SODAS, particularly to the courtesy of their respective owner and Mr Cihat Kora who permitted us to access and work on the Acıgöl Core-3. Moreover, Mr Hüseyin Ünlü, the general director of ALKİM Company during the coring, Mr. Ünal Arık director of ALKİM and Mr. Mahmut Eser, the correspondent of the core is greatly appreciated as well as Mr. Serdar Salancı, the general director, and Mr. Necati Demirer the director of SODAS Çardak Plant. We thank Dahvya Belkacem for having extracted the pollen from the series of Acıgöl and Kocabaş. We thank Michelle Leydet for storing our pollen data in EPD. We thank Alain Tonetto and the Platform for Analytical Technological and Imaging Research (PRATIM), from Aix Marseille University, for the confocal photographies of the pollen. Finally, we would like to thank Dr Chenglong Deng (Editorial Board Member of Scientific Reports) and the reviewer, Dr Li Quan, for very pertinent remarks, which helped to improve the manuscript significantly.

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V.A-.P., P.R. and M.C.A. designed research with the participation of all the co-authors F.D., H.A., N.B., D.B., C.H., A-.E.L., S.M., H.M., A-.M.M., S.N., M.P., P.P., C.R., A.V., B.G. Pollen analyses were carried out by V.A-.P. Floristic relevés for the current pollen rain were carried out by H.M., P.P. and V.A-.P. The magnetostratigraphy was carried out by F.D. and P.R. The site stratigraphy and the depositional processes were analysed by C.M., C.H., F.D., H.A. P.R. and M.C.A. V.A-.P. supervised the writing of the article.

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Correspondence to Valérie Andrieu-Ponel.

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Andrieu-Ponel, V., Rochette, P., Demory, F. et al. Continuous presence of proto-cereals in Anatolia since 2.3 Ma, and their possible co-evolution with large herbivores and hominins. Sci Rep 11, 8914 (2021). https://doi.org/10.1038/s41598-021-86423-8

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