BoxA blocks ICM-induced shift in NG2 cells from stellate to reactive morphology. (A) Representative images of NG2+ cells in experimental cultures in a resting, stellate morphology. Note the radial pattern of numerous processes emerging from a homogenously stained cell body. (B) Representative images of NG2 cells in experimental cultures in a reactive morphology. Note the linear pattern of a much-reduced number (2–4) of processes emerging from a heterogeneously stained, swollen cell body. (C) In the presence of 100 ng/ml disulphide HMGB1, There were a significantly higher proportion of reactive cells (Chi-square 110.4, 1; p < 0.0001). (D) in the presence of injury conditioned media, there was again a significantly increased proportion of NG2+ cells with a reactive morphology (Chi-square 77.73; 1; p < 0.0001). Furthermore, addition of BoxA to ICM cultures reduced the proportion of reactive cells to that of control cultures (Chi-square 82.72, 2; p value < 0.0001). For comparisons between two different conditions, a two-sided Chi2 test was used in panels C-D. p values of < 0.05 were considered significant (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). cNSPCs = rat cortical neural stem cell progenitors; DIV = days in vitro.