Figure 1 | Scientific Reports

Figure 1

From: Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium

Figure 1

Effects of different lithium concentrations on BMSCs proliferation and osteogenic differentiation. (A) Characteristics of BMSCs. Results of immunofluores- cence labeling of third-generation bone marrow mesenchymal stem cells showed positive CD29 (A), CD44 (B), CD90 (C) and CD271 (D), and negative CD11b (E) and CD45 (F). (B) The multiplication time of BMSCs in the medium containing lithium salts of different concentration gradients, compared with the control group *P < 0.05; (C) Optical density (OD) of BMSCs cultured in osteogenic medium containing lithium salts of different concentration gradients for 21 days in ALP staining, compared with the control group *P < 0.05. (D) Expression of osteocalcin after BMSCs were cultured in osteogenic medium containing lithium salts of different concentration gradients for 21 days, compared with control group *P < 0.05. (E) ALP expression of BMSCs cultured in osteogenic medium containing lithium salts of different concentration gradients for 21 days compared with control group *P < 0.05.Three fields of view were taken for each group, and the data were plotted in the format of mean ± standard deviation.

Back to article page