Electrophoresis analysis of the NP corona complexes. (a) Agarose gel electrophoresis assessed in a 2% (w/w) agarose gel of 5 nm AuNP before and after exposure to human plasma. The gel was visualized by a visible light scanner. (b) Silver stained SDS-PAGE gel showing the protein profile of the associated coronas for the different Au-PMA core sizes. The two lanes for each of the samples correspond to biological replicates, and the last one (C) represents the protein background (negative control). SDS-PAGE protein ladder corresponds to ColorPlus Prestained Protein Ladder, Broad Range (10–230 kDa) (New England Biolabs).