Table 1 Summary of previous anthozoan cell culture publications.

From: Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis

Publication Model organism(s) Notes Maximum viability* period
Barnay-Verdier et al. 2013 Anemonia viridis Tentacle tissue only 14 days
Domart-Coulon et al. 2001 Pocillopora damicornis Calcium carbonate formation N/A
Domart-Coulon et al. 2004 Stylophora pistillata, Pocillopora damicornis Multicellular isolates 2 weeks
Domart-Coulon et al. 2004 Pocillopora damicornis Calcium carbonate formation 6 days
Downs et al. 2010 Pocillopora damicornis Early microbial interference N/A
Drake et al. 2018 Stylophora pistillata Early microbial interference N/A
Estephane and Anctil 2010 Renilla koellikeri Cell dedifferentiation reported 10 days
Frank et al. 1994 Dendronephthya hemprichi, Heteronexia fuscescence, Favia favus, Plexaura sp., Stylophora sp., Clathraria sp., Parerythropodium sp., Milleopora sp., Porites sp. Early microbial interference N/A
Helman et al. 2008 Xenia enlongata, Montipora digitata Calcium carbonate formation N/A
Khalesi 2008 Sinularia flexibilis Early microbial interference N/A
Kopecky and Ostrander 1999 Acropora micropthalma, Pocillopora damicornis, Stylophora pistillata, Seriatopora hystrix, Porties sp. Multicellular isolates N/A
Lecointe et al. 2013 Pocillopora damicornis Multicellular isolates 7 days
Mass et al. 2012 Stylophora pistillata Calcium carbonate formation N/A
Mass et al. 2017 Stylophora pistillata Calcium carbonate formation 7 days
Nesa and Hidaka 2009 Fungia sp., Pavona divaricata Multicellular isolates 3 days
Rabinowitz et al. 2015 Nematostella vectensis Ectodermal tissue layers 19 days
Reyez Bermudez and Miller 2009 Acropora millepora Larvae-derived 11 days
Ventura et al. 2018 Anemonia viridis Tentacle tissue only 30 days
  1. Previous publications on anthozoan cell culture: A review of past publications that produced cnidarian cell cultures .“Notes” column indicates particular tissues preparations that were used in each study. Maximum viability is what was reported in the respective publication. “N/A” is marked for publications where viability was impossible to determine given the results. (*although the publication may report a viable time period, there was either some contamination, cell loss, or loss of cell diversity during the reported time, meaning at an unknown point the cultures were not “viable” by the standards put forth in this paper).