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Figure 3

From: Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis

Figure 3

Cnidarian cell culture cell type diversity. (A) N. vectensis cells 48 h pd with several intact nematosomes (white arrowhead). (B) N. vectensis cells isolated only from the mesenteries 6 days pd. This cell suspension had mostly granulated round cells with dark vacuoles (black arrowhead) and large, occasionally mobile round clusters (white arrow). (C) Cells isolated only from the oral region 6 days pd. A diverse suspension of unidentified smaller round ectodermal cells (black arrowhead) along with cnidocytes (white arrowhead). (D) Proliferative N. vectensis cell culture 7 days pd with diverse cells of various shapes and sizes, including “pointed” round cells (white arrowhead) and globular cells, mostly unidentifiable based on morphology. (E) P. damicornis cell culture 21 days pd with low zooxanthellae counts, giving an observable variety of mostly unidentified host cnidarian cells in culture such as abundant rounded cells (arrowhead) (F) Diverse P. damicornis culture 25 days pd with smaller putative digestive round cells (arrowhead). (G) P. damicornis culture 28 days pd with 3 types of viable cnidocytes (arrows) (along with a piece of coral aragonite in frame) (H) P. damicornis culture 6 days pd with a large discharged cnidocyte (arrow).

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