Cnidarian cell culture growth and viability. (A) Distribution of N. vectensis cell culture longevity over 123 cell cultures (n = 123, binwidth = 2 days). The dashed vertical lines indicate the 95% confidence interval after a one sample t-test (11.41879, 13.39421). (B) Histogram of distributions of P. damicornis cell culture longevity over 51 cell cultures (n = 51, binwidth = 2 days). The dashed vertical lines indicate the 95% confidence interval after a one sample t-test (10.79539, 14.53794). (C,D) Comparisons of viability between culture media in N. vectensis and P. damicornis. Each grey rectangle represents the 95% confidence interval for viable days in culture for all recorded cell cultures using the listed media. Two-tailed t-test between each medium were performed, a and b represent significantly different intervals (*** = P < 0.001) (ACCM anemone cell culture medium, CCCM coral cell culture medium, FSM full strength medium, AGM anemone gentamicin medium, CGM coral gentamicin medium, FDL fish disease lab media, see supplementary figures for media formulae). (E,F) Comparison of cells counts for N. vectensis and P .damicornis maintained in seawater (11ppt seawater for N. vectensis) or cell culture media over a 14-day period. Cell counts were plotted as log10 in cells/well of culture against time (in days). Statistical significance was determined using a non-parametric Kruskal–Wallis between counts on days 0 and 7, 7 and 14, and 0 and 14 for both species. (***P < 0.001, **P < 0.01).