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Figure 1

From: Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis

Figure 1

Dissociation process of N. vectensis and P. damicornis tissue. (A) MgCl2 treated N. vectensis with an initial scalpel cut for mechanical dissociation. (B) N. vectensis tissue clump being spontaneously dissociated into cells in culture 24 h post-dissociation (pd). (C) Diverse N. vectensis cell suspension after full dissociation 48 h pd. (D) Green autofluorescent P. damicornis tissue being sloughed off of the skeleton 8 h into antibiotic-facilitated dissociation. (E) P. damicornis cells immediately after dissociation with intact bailed-out polyps (black arrowhead) and abundant zooxanthellae (white arrowhead). (F) Autofluorescent P. damicornis cells 12 h post-dissociation with various cell types. Red fluorescence indicates algal symbionts, Symbiodiniaceae.

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