A small-molecular inhibitor against Proteus mirabilis urease to treat catheter-associated urinary tract infections

Infection and blockage of indwelling urinary catheters is significant owing to its high incidence rate and severe medical consequences. Bacterial enzymes are employed as targets for small molecular intervention in human bacterial infections. Urease is a metalloenzyme known to play a crucial role in the pathogenesis and virulence of catheter-associated Proteus mirabilis infection. Targeting urease as a therapeutic candidate facilitates the disarming of bacterial virulence without affecting bacterial fitness, thereby limiting the selective pressure placed on the invading population and lowering the rate at which it will acquire resistance. We describe the design, synthesis, and in vitro evaluation of the small molecular enzyme inhibitor 2-mercaptoacetamide (2-MA), which can prevent encrustation and blockage of urinary catheters in a physiologically representative in vitro model of the catheterized urinary tract. 2-MA is a structural analogue of urea, showing promising competitive activity against urease. In silico docking experiments demonstrated 2-MA’s competitive inhibition, whilst further quantum level modelling suggests two possible binding mechanisms.

www.nature.com/scientificreports/ and recurrent urinary tract infection by P. mirabilis [14][15][16][17] . However, AHA is known to induce severe side effects including teratogenesis and hemolytic anaemia 15,[18][19][20] . Awareness of such undesirable side effects has resulted in a fall in AHA popularity in recent years, inducing a surge of research into alternative urease inhibitors with less severe toxicological profiles. Targeting urease as a therapeutic candidate facilitates the disarming of bacterial virulence without impacting bacterial viability. Thereby limiting the evolutionary selection pressure placed on the invading bacterial population (since bacteria are not inhibited or destroyed by the therapeutic approach). Small molecular enzyme inhibitors, often based on the molecular structure of the native substrate of the enzyme have proved promising in rational drug design, with bacterial enzymes deemed effective targets for therapeutic intervention in human bacterial infections 21 . Enzymes associated with P. mirabilis virulence have previously been targeted as therapeutic options. Carson et al., previously described the development and analysis of a library of N-alpha mercaptoamide dipeptide inhibitors against Zap A protease (a virulence factor known to play a key role in P. mirabilis pathogenesis) 22 . Despite their in vitro success in inhibiting ZapA, these inhibitors showed negligible activity against urease (unpublished data, not shown). Therefore, a design is proposed whereby the bulky, peptide-containing regions of the inhibitor are eliminated, leaving only the thiol-containing amide 'warhead' of the molecule, 2-mercaptoacetamide (2-MA) (Fig. 1a). The conserved thiol moiety of the inhibitor shows structural potential for the inhibition of urease. Since there are structural similarities with urea, there is potential to allow for a binding mode that resembles that of the native enzyme-substrate complex and the consideration of the innate substrate specificity of the urease enzyme. Furthermore, the proposed 2-MA inhibitor contains a thiol moiety, which is known to display active inhibition of the urease enzyme, as described elsewhere 23,24 . Urease inhibitors have been extensively examined by previous researchers as described by 25,26 . However, many focus on their use in agriculture and few test their use on medicinally relevant bacterial ureases such as those from P. mirabilis.
Here, we describe the preparation and in vitro evaluation of 2-MA as a small molecular urease inhibitor, which can prevent encrustation and blockage of urinary catheters in a physiologically representative in vitro model of the catheterized urinary tract. Computational docking experiments identify 2-MA as a competitive inhibitor against urease and shows the possibility for rational design of future inhibitory candidates. Furthermore, quantum mechanical (QM) computational modelling hypothesizes two possible 2-MA's binding mechanisms and allows comparison to the licensed drug AHA.

Results and discussion
This study aimed to identify an inhibitor of the urease enzyme, which is produced by the uropathogenic bacteria P. mirabilis. Inhibition of this target was intended to disarm P. mirabilis's ability to form crystalline biofilms, thus preventing encrustation and blockage of long-term urinary catheters.
Enzyme kinetics. Urease from Canavalia ensiformis (C. ensiformis) was used as a model urease to determine the kinetics and analyze the initial inhibitory affinity of 2-MA. The active site of urease is conserved between C. ensiformis and P. mirabilis urease ( Supplementary Fig. 3). Michaelis-Menten analysis of enzyme kinetics showed that a sigmoidal curve fit agreed with the observed kinetic data (R 2 = 0.9959) compared with a hyperbolic curve fit (R 2 = 0.9537) (Fig. 1b,c).
The V max was calculated as 1.31 × 10 -2 M and the K M as 0.16 M (Fig. 1c). The K M , 0.16 M, was compared to literature values of 2.7-8.81 mM 27 . A Hill coefficient of 2.19 suggest cooperative binding of urea to urease. This agrees with literature on the mechanism of urease where both a nucleophilic water molecule and urea bind to the bi-nickel active site, this was further confirmed through computational analysis 28 . Analysis of Michaelis-Menten parameters in the presence and absence of 2-MA indicates the type of inhibition (Supplementary Table 1). The V max remains approximately the same upon addition of 2-MA (within the error margins), whilst K M increases suggesting decreased affinity of urea for the active site urease, indicating that 2-MA is binding competitively and thus is competing directly with the substrate for the active site (Supplementary Table 1). It is worth noting, however, that there are limitations to the Michaelis-Menten model. Whilst it has been used widely for over a century to estimate enzyme kinetic parameters from substrate reaction curves, this canonical approach only works in limited conditions (such as when there is a large excess of substrate over enzyme). Even when this condition is satisfied, the identifiability of parameters is not always guaranteed, and often not verifiable in practice 29 . Furthermore, molecular docking experiments suggested that 2-MA was binding within the active site and thus is competitively inhibiting urease. The mechanism of enzyme inhibition, as well as the inhibitor constant (K i ) were confirmed via the linear graphical method known as the Dixon plot. The effect on the enzymatic initial rate (V 0 ) is determined at two or more substrate concentrations over a range of inhibitor concentrations. For competitive inhibition, a plot of 1/initial rate vs. inhibitor concentration will yield a series of straight lines that intersect above the X-axis; the abscissa at which they converge represents the − K i 30 . This provides a quantitative measure of inhibitory potency that is independent of substrate concentration, and provides information about the binding affinity of an inhibitor 31 . Using the Dixon method, it was seen that 2-MA competitively binds to urease (Fig. 1d). The K i was calculated as 2.95 mM, the K i of AHA for urease has been previously described as 5.7 ± 0.4 μM, and 0.053 mM, for C. ensiformis and P. mirabilis urease, respectively 32,33 . Therefore, 2-MA is less potent than AHA however, AHA causes severe toxicity to the patient and 2-MA efficacy in preventing blockage of catheters also needs to be examined. Half-maximal inhibitory concentration (IC 50 ) is the inhibitor concentration at which the activity of the enzyme is reduced by 50%, when compared to the activity of the enzyme in the absence of the inhibitor. Determination of IC 50 of a compound provides a further measure of the antagonistic potency, in a manner independent of defined experimental conditions (such as substrate concentration). Dose-response curves and subsequent determination of IC 50 for both 2-MA and AHA are shown in Fig. 1e Fig. 3). The crystal structure of urease (protein data bank (PDB): 4UBP) from Sporosarcina pasteurii (S. pasteurii) (formally Bacillus pasteurii) was chosen owing to its co-crystallization with AHA and high crystal resolution (1.55 Å), thus allowing accurate determination of bond distances in docked compounds 34 . We performed 'self-docking' , whereby crystallized AHA was used as a control to check the computational docking before docking the 2-MA. Figure 2a indicates that docked AHA reported good confirmation to the crystalized AHA, with a root mean squared deviation (RMSD) of 0.387 Å, where an RMSD of < 2 Å is considered a 'well-docked' ligand 35 . It was concluded that the docking software was performing as expected and would provide realistic docking for 2-MA. Compounds were molecularly docked into the active site of urease and their contacts with the protein examined. For protein-ligand interactions, hydrogen bonding as well as hydrophilic-hydrophobic interactions are important for drug binding. Owing to the metallocentre of urease, Ni ions can also be involved in coordinating ligands, such as that observed in thiol inhibitors 36 . By examining the distances between the compound and the protein, hydrogen bonding can be predicted, most hydrogen bonds have a bond distance between 2.7 and 3.1 Å 37 . Urea was docked into the active site, it coordinates with Ni ions, with bonding distances calculated to be 1.8 Å to Ni(1), and 2.3 Å to Ni(2) (Fig. 2b). Urea also binds to D363 at a distance of 2.9 Å, as well as a water molecule at 2.8 Å. The binding contacts of urea with the active site of urease, agreed with the mechanism of urease proposed by Amtul et al. 28 . Figure 2c shows docked 2-MA. This is in agreement with the Dixon Plot and Michaelis-Menten parameters in the presence of 2-MA, to confirm that 2-MA is inhibiting urease by competitive inhibition (Fig. 1d, Supplementary Table 1). The carbonyl group appears to bind with amine from H222, with a bond distance of 1.9 Å. The amine from 2-MA forms hydrogen bonds with H275 with a bond distance of 2.6 Å, and D363 with a bond distance of 1.9 Å. The sulfur forms hydrogen bonds with two water molecules found in the active site, upon comparison to the crystallized structure of urease with β-mercaptoethanol, it is observed that the sulfur is not bound with the Ni ions as observed in the crystallized structure 38 . It could be hypothesized, that the amide group is more favorable to chelating the Ni ions compared to the sulfur as it is a substrate mimic. However, crystallography studies are required to further confirm this 2-MA binding behavior. It is hypothesized that the potency of 2-MA could be improved by chain extension from the methylene group to the develop contacts with the surrounding amino acids. Furthermore, 2-MA conserves the amide functional group from urea therefore, superposition of urea and 2-MA determined that both compounds dock in the same orientation (Fig. 2d). Both the carbonyl and the amide group of urea and 2-MA coordinate to the Ni ions from urease. The docking score uses mathematical methods to predict compounds docking pose to a receptor 39 . Whilst the docking score alone does not provide an accurate model of binding, when combined with successful self-docking data, it can be used successfully to analyze compound analogues 40 . The docking scores for urea, 2-MA, and AHA were − 3.814, − 6.347, and − 6.534, respectively. The docking score for urea is higher than 2-MA and AHA, suggesting that www.nature.com/scientificreports/ the compounds are binding better than the natural substrate. This is likely due to the higher amount of contacts 2-MA and AHA are making within the active site. 2-MA and AHA have very similar docking scores indicating a similar binding strength to the active site. However, AHA has a lower score than 2-MA, thus the docking scores agree with the kinetic data, suggesting that AHA has a higher affinity than 2-MA for urease's active site.
QM modelling of S. pasteurii urease active site. An inherent limitation with a classical molecular docking approach is that one cannot account for QM effects such as bond breaking and formation, nor observe directional binding to metal centers, which arise as a direct result of orbital overlap. To this end, further QM computational studies were undertaken to supplement the docking analysis. Structures were selected based on molecular docking configurations and crystal structure data. These structures were then optimized to potential energy minima. Figure  Potential energies for these structures can be found in Table 1. It must be noted that the energies presented in this report are enthalpies of binding, as opposed to free energies, upon which true binding will depend. It is therefore important that enthalpies are compared for binding events where the entropy change is likely to be similar, such as for a binding process which liberates the same number of small molecules (i.e. water). When this is not the case, one must be aware that enthalpy alone, whilst a good indicator for binding affinity, does not provide the full picture that the free energy of binding would. The binding enthalpy of urea in Fig. 3a is − 77.7 kJ mol -1 , which is similar to the binding enthalpy of monodentate bound 2-MA in Fig. 3b, − 64.6 kJ mol -1 . In this mode of binding, urea is predicted to be a better ligand than 2-MA by roughly 13 kJ mol -1 . This suggests that this is not the binding mode that 2-MA adopts in the active site, as it is energetically favorable for urea to displace 2-MA in this mode. Attempts to optimize cross chelating structures of urea and 2-MA (Ni(1)-O=C and Ni(2)-NH 2 ), in a fashion similar to that shown by the substrate analogue, boric acid 44 , led to attack by the bridging hydroxide on the electrophilic carbonyl. This concerted cross coordination and nucleophilic attack mechanism is supported by previous observations and calculations made by Musiani et al. on the mechanism of urease catalysis 45 . This nucleophilic attack by the bridging hydroxide leads, in both cases, to a tetrahedral intermediate, as seen in Fig. 3c,d. When bound in this fashion the 2-MA ligand is 28 kJ mol -1 more stable than urea. Reaction via a tetrahedral intermediate as the mechanism for urease has also been supported by recent X-ray crystallographic data on the urease-urea complex by Mazzei et al. 42,46 . The difference in binding energies suggest that in this binding mode, 2-MA is favored over urea and would lead to inhibition of enzyme function. Structures in Fig. 3e,f are the optimized structures of the AHA anion bound to the urease active site, as determined from crystallographic data, and the 2-MA anion mimicking this binding mode 34 . Comparison of the binding energies of these structures shows that this mode of binding is highly unfavorable for 2-MA. Noticeably, the binding enthalpy for AHA in Fig. 3e is calculated as 70 kJ mol -1 higher than that of urea in Fig. 3a. Whilst this suggests that urea binding in Fig. 3a is enthalpically favorable when compared to AHA binding, when compared to urea binding in a tetrahedral intermediate (Fig. 3c), which is believed to be an intermediate along the urease mechanistic pathway, AHA has a favorable binding enthalpy of approximately 30 kJ mol -1 . Both in silico studies  Investigating 2-MA inhibition on P. mirabilis urease. Evaluation of the functionality of 2-MA against urease from P. mirabilis is crucial for understanding whether 2-MA is effective in a clinical scenario. Urease is a intracellular enzyme, thus 2-MA must be able to cross the bacterial membrane 6,53,54 . Analysis of the growth of P. mirabilis (strain B4) in the presence of 2-MA and AHA allows the maximum tolerable concentration of the drug to be determined, by comparison to the B4 control (untreated control). The maximum tolerable concentration is defined as the concentration of the drug at which the growth of P. mirabilis remains unaffected. By maintaining bacterial cell viability, evolutionary selection pressures are not introduced, thus the likelihood of resistance occurring decreases 21,55 . It was determined using a standard microbroth dilution method that the maximum tolerable concentration for both 2-MA and AHA is 10 mM (Supplementary Fig. 4). For in vitro whole-cell urease evaluation, it was imperative to employ a concentration of drug that does not affect bacterial cell viability, such that any reduction in urinary crystal formation may be attributed solely to urease inhibition, not a reduction in bacterial bioburden. Therefore, the selected dosage for all further biological evaluation was 10 mM for both 2-MA and AHA. Penetration through the cell membrane provides a significant challenge in the design of small molecular drugs such as enzyme inhibitors. Intracellular uptake is frequently confirmed via measurements of a biological effect generated only when the payload is successfully delivered to the cytoplasm. Macromolecular changes in pH, over a small time period, were used to assess whether 2-MA was passing through the membrane of P. mirabilis (Fig. 4). Changes in pH caused from the production and accumulation of ammonia in the urine were used as a proxy indicator of enzyme activity. Comparison to a uropathogenic strain of Escherichia coli (E. coli), a urease-negative control, indicates that the change in pH is as a result of urease activity from P. mirabilis. The measured pH (after 90-min incubation) between P. mirabilis and E. coli was found to be significantly different (p = 0.0001) (Fig. 4A,B). Exposure of P. mirabilis to 10 mM 2-MA prevented urinary pH elevation compared to an untreated control (p < 0.001) (Fig. 4A). Urease inhibition in the presence of 2-MA (10 mM) was comparable to AHA (10 mM), both compounds exhibited significant enzyme inhibition. There was no alteration in urinary pH for cultures of urease-negative E. coli (Fig. 4B). Furthermore, there is no significant difference in the bacterial viability between P. mirabilis and E. coli at the start and the end of the experiment for either of the drugs tested (Fig. 4C,D). In vitro bladder model testing. The efficacy of 2-MA in a clinically representative system was assessed using in vitro bladder models, as previously described 56 . Physiological conditions (including temperature and urine flow rate) were maintained within the bladder models, which were inoculated with P. mirabilis and E. coli at 10 8 colony forming units (CFU)/mL to model a late stage CAUTI. 2-MA and AHA drugs were added by direct dissolution into the urine reservoirs at 10 mM concentration. The blockage of urinary catheters and subsequent termination of urine flow was defined as the experimental end point (Fig. 5a). The performance of 2-MA was compared directly to that of AHA, in order to reference the extension of catheter lifetime under the same experimental conditions. Periodic measurements of bladder conditions (pH and CFU/mL) were performed throughout the experiment, to monitor the urease activity as a function of residual urine pH, as well as the viability of colonizing bacteria (Fig. 5b,c). Both inhibitors achieved significant extension of catheter lifetime compared to the untreated control. When urease activity was uninhibited, blockage of control models occurred after 15 h. Treatment with AHA extended average blockage time fivefold, blockage was visually observed at 74 h (p < 0.0001). Surprisingly, treatment of P. mirabilis-infected models with 2-MA further extended catheter lifetime within the in vitro model, such that blockage was not observed throughout the 5-day duration of the Table 1. Potential and binding energies of structures Fig. 3a-f. a The number of water molecules exchanged with the ligand upon binding to the wild type active site. b Potential energies are quoted for the unrestricted Becke, 3-parameter, Lee-Yang-Parr (B3LYP) 47-50 functional and 6-31G*/SDD basis sets 51,52 (UB3LYP/6-31G*/ SDD). c Binding energies were calculated as potential energy of the structure plus the potential energies of any liberated water molecules (aqueous solvation with PCM, ε = 80), minus the potential energies of the wild type active site and aqueous solvated ligand (PCM, ε = 80). All calculated energies can be found in Supplementary www.nature.com/scientificreports/ experiment. Reservoirs of artificial urine media were exhausted 120 h after model start, at which point catheters treated with 2-MA were still draining freely (Fig. 5a). This in vitro model shows the ability of 2-MA to significantly outperform AHA, the only currently marketed urease inhibitor. 2-MA successfully prevented blockage of the catheters and significantly extended the lifetime of the catheter by an extra 46 h compared to AHA. Within the untreated control models, the pH of artificial urine media within the bladder rapidly increased, owing to the urease-mediated urea hydrolysis within the P. mirabilis population. The pH of residual media was elevated to pH 8 within 5 h of the experiment start. This was accompanied by an increase in viable bacterial cells within the bladder, since urease expression was able to facilitate rapid colonization and biofilm formation. Local supersaturation and precipitation of crystalline materials within the catheter lumen and eyehole region therefore elicited total occlusion of the catheter within 15 h. In contrast, pH elevation within treated models was unable to surpass to nucleation point (≈ pH 7.4) until approximately 45 and 108 h for AHA and 2-MA, respectively. Eventual elevation of urine to pH 8 in AHA-treated models was accompanied by catheter blockage by crystalline biofilm formation. Models treated with 2-MA, were unable to reach pH 8 within the experimental term, thus the degree of crystalline biofilm formation was insufficient to result in catheter blockage (Fig. 5b). Both the AHA and 2-MA-treated models underwent an approximately 1-log reduction in viable cell count within the first 24 h of the experiment. P. mirabilis virulence factors (particularly urease) are known to play an extensive role in bacterial pathogenesis and biofilm formation 4 . It is therefore unsurprising that inhibition of urease activity manifested as a decrease in bacterial colonization. It has been shown that urease-negative mutants, colonize the urinary tract 100-fold less efficiently than the parent strain 57,58 . It is likely that the reduction in adherence resulted in direct elution of a large portion of the bacterial population from the bladder in the early stages of infection (Fig. 5c). Disparities in the degree of encrustation on catheter luminal surfaces at time of AHA-treated model blockage (74 h) and unblocked 2-MA-treated model (120 h) were observed visually. Total occlusion of the eyehole and drainage lumen were observed in the catheter removed from the blocked AHA treated model ( Supplementary  Fig. 5a). Significantly less crystalline material was observed within the catheter removed from the 2-MA treated model ( Supplementary Fig. 5b). www.nature.com/scientificreports/ In order to further elucidate why 2-MA has outperformed AHA despite having a lower potency in initial IC 50 studies, the ability of 2-MA to inhibit the formation of P. mirabilis biofilms was investigated using a simple in vitro crystal violet staining model. Since treatment of the in vitro catheterized bladder with 2-MA resulted in significant elution of planktonic cells from the bladder in the early stages of the experiment, it was hypothesized that this drug is preventing the early colonization of a biofilm on the catheter's luminal surfaces. The ability of both enzyme inhibitory drugs to prevent biofilm formation of P. mirabilis is shown in Fig. 6. At the therapeutic concentration of 10 mM, 2-MA showed significantly greater ability to prevent formation of biofilms than AHA (p < 0.0001), although it did not completely prevent formation (p = 0.0214).
Since previous investigation into the antimicrobial susceptibility of P. mirabilis to both 2-MA and AHA has confirmed that 10 mM concentrations are unable to induce bacterial cell death ( Supplementary Fig. 4), it appeared that the reduction in biofilm formation (and hence extension of catheter lifetime) is owing to the prevention of catheter colonization and adherence to the catheter surface.
Cytotoxicity testing. Within cell culture systems, a compound is considered cytotoxic if it interferes with cellular attachment, significantly alters morphology, adversely affects growth rate, or causes cell death 59 . Cytotoxicity can be measured by measuring cellular metabolism, 2-MA was directly compared to that of AHA using a HaCaT immortalized keratinocyte cell line using the XTT cell metabolism assay. 2-MA consistently showed significantly less cytotoxicity than AHA. At 10 mM, which has been used to treat the P. mirabilis, reduced viability is observed for both AHA and 2-MA. Investigating the cytotoxicity against HaCaT cells indicated that, as previously observed by Williams et al., AHA was toxic towards mammalian cells (Fig. 7a) 18 . Incubation of cells with 10 mM 2-MA resulted in 60% cell viability, whereas under the same conditions AHA resulted in a decrease to 30% viability. Any reduction in viability of HaCaT cells is discouraging in drug development however, 2-MA offers a good starting point for further optimization. The cytotoxicity of AHA agrees with the literature and has also been reported to behave synergistically with bacterial virulence factors leading to increased toxicity 60 .
The degree of hemolytic anemia induced by a drug may be evaluated ex vivo by assessing the dose-dependent liberation of hemoglobin from human red blood cells via the measurement of absorbance at 415 nm. Obtained www.nature.com/scientificreports/ values were corrected against media controls, and degree of hemolysis expressed as a percentage of the positive lysis control (Triton X-100). Percentage hemolysis for both 2-MA and AHA (both dissolved in phosphate-buffer saline (PBS)) at varying concentrations is shown in Fig. 7b. Toxic hemolytic activity was significantly lower for 2-MA than AHA (p < 0.0001). Of particular relevance was the result at the defined therapeutic concentration (10 mM), where % hemolysis was observed to be 10.7% ± 0.8 for 2-MA; an 89% reduction from the value observed for AHA at the equivalent concentration. Even at the highest evaluated concentration of 40 mM, observed hemolysis was still 78% lower following treatment with 2-MA in comparison to AHA (p < 0.0001 for all comparative concentrations) ( Supplementary Fig. 6).

Conclusions
Drugs that function as enzyme inhibitors constitute a significant portion of the orally bioavailable therapeutic agents that are in clinical use today. The 'disarming' of potent bacterial virulence factors without placing a selective pressure on the cells themselves comprises a powerful therapeutic approach in light of the dawn of the post-antibiotic era. Kinetic and in silico analysis indicates that 2-MA is a competitive inhibitor against urease. 2-MA was shown to out-perform AHA during in vitro bladder model evaluation and cytotoxicity studies. QM computational analysis identified two possible competitive binding mechanisms for 2-MA: either mimicking monodentate urea binding or binding as a tetrahedral intermediate. Of these two modes, the tetrahedral intermediate is likely to contribute to greater inhibition of urease action based on our calculations. The licensed inhibitor, AHA, has a higher affinity for urease, however this comes at a significant cytotoxic cost, both to bacterial and mammalian cells 17,19 . The trade-off between toxicity and potency is common in drug discovery; multi-optimization objective methods have been developed to rank compounds that fully agree with multiple criteria 61 . This is in contrast to traditional methods where objects are tackled sequentially, normally starting with potency 62 . We have analyzed 2-MA for multiple criteria, including potency and toxicity as well as examining its binding mechanism. The relatively low toxicity, low cost, and efficacy of 2-MA not only in inhibiting urease but also, in inhibiting biofilm formation suggest that 2-MA may have clinical utility in preventing urinary catheter  www.nature.com/scientificreports/ blockage. Although the working concentration of 10 mM 2-MA used in this study is high, direct bladder washouts using a three-way Foley catheter, means that 2-MA could be introduced as a weekly prophylaxis in the clinic, replacing AHA. 2-MA additionally offers a suitable platform from which more potent analogues may be developed through the use of rational drug design.

Methods
General remarks. Solvents and reagents were purchased from commercial suppliers and used without further purification unless otherwise stated. 1 H NMR was recorded on a Bruker 400 MHz. Chemical shifts (δ) are reported in ppm, and referenced to residual solvent peaks. Splitting patterns are defined as singlet (s), doublet (d), triplet (t), quartet (q), multiplet (m) or broad (br) where appropriate. The HPLC-ESI-TOF analysis was conducted using an electrospray time-of-flight (MicrOTOF) mass spectrometer (Bruker Daltonik GmbH, Bremen, Germany), which was coupled to an Agilent HPLC stack (Agilent, Santa Clara, CA, United States) consisting of Agilent G1312A binary pump with G1329A autosampler and G1316A column oven. Analyses were performed in ESI positive mode with capillary voltage at 4500 V, nebulizing gas at 2.2 bar, drying gas at 10.2 L/min at 220 °C. The TOF scan range was from 50 to 750 mass-to-charge ratio (m/z). swarming ability, to achieve single colonies, non-swarming LB (NSLB) composed of tryptone (10 g/L (Merck, Germany)), yeast extract (5 g/L (Merck, Germany)) and bacteriological agar (15 g/L (Merck, Germany)) is required. P. mirabilis NSLB plates were incubated the same as E. coli. Single colonies were picked from the plates and grown in 10 mL of LB broth overnight at 37 °C with shaking (200 rpm). Quantification is carried out using the Miles, Misra and Irwin method 65 . Plates are incubated at 37 °C statically overnight. CFU/mL were counted where d is the dilution factor, and V is volume of inoculum (mL).
Colorimetric urease quantification. To access the activity of urease, a colorimetric assay developed by Onal Okyay and Frigi Rodrigues, was used 66 . In the presence of urea, SB undergoes a color change from yellow (Abs max 430 nm) to pink (Abs max 560 nm) occurs. Three biological repeats were completed using a microplate reader (BMG Labtech) with an inbuilt injector system to analyze initial rate of color change.

Michaelis-Menten Plots & Dixon
Plot. Kinetic assays were prepared by adding 100 μL SB (minus urea) and 100 μL urease (urease from C. ensiformis (Type III powder, 15,000-50,000 units/g (Merck, Germany)) (135 U in PBS, determined above) to a 96-well plate (Corning, New York, USA). To the injector system of the plate reader, SB (plus urea (Merck, Germany)) was added (in excess). Each well was read at 560 nm to achieve cycle time of 6 s, each well was read for a total of ten cycles. www.nature.com/scientificreports/ Curve fitting and statistical analysis was performed using GraphPad Prism (version 7, GraphPad Software Inc., San Diego, CA). Kinetic models (Michaelis-Menten) were fitted using non-linear regression and SEM, 95% confidence intervals and R 2 values were assessed.
Measuring IC 50 . To determine IC 50 the Burt, Galetin and Houston protocol was followed 67 . Urea (20 mg/mL in standard SB) and urease (135 U in PBS) were remained constant. Compounds (2-MA and AHA (Merck, Germany)) were varied from (0-900 mM in PBS) and the degree of color change from yellow to pink, was accessed over a 10 min incubation. Compound solution (100 μL) and urease solution (100 μL) were added to 96-well plate (Corning, New York, USA). SB broth (100 μL) was added to the 96-well plate using the plate reader injection system. Inhibition was monitored at 560 nm for 1 h (60 s cycle time for ten cycles). The enzyme response in absence of compound was defined as 0% inhibition (Max) and SB with no enzyme was maximum inhibition (Min). % Inhibition at each inhibition concentration (x) was calculated according to the equation: Max − Min . The data was fitted with sigmoidal dose-response curves and IC 50 determined by non-linear regression using GraphPad Prism Version 7.
In silico analysis of 2-MA binding. Docking experiments using S. pasteurii urease. The crystal structure of S. pasteurii urease, 4UBP 34 , was used for docking analysis. The protein was prepared using Flare (Flare 3.0.0, Revision 38710; Cresset, Litlington, Cambridgeshire, UK). A docking grid was determined by a 10 Å radius around the Ni (1) in the active site. Docking was carried out by Flare, the ligands were docked using the 'accurate but slow' setting. Docking score was determined by Flare, using the LF Rank Score.
QM modelling of S. pasteurii urease active site. The system of S. pasteurii urease was prepared based on the crystal structures of AHA inhibited and wild type S. pasteurii urease with PDB IDs 4UBP 34 at 1.55 Å resolution and 2UBP 68 at 2.00 Å resolution, respectively. Hydrogen atoms were added using the fDynamo computational library of Field et al. 69 . Residues were selected according to Nordlander et al. 41 . Systems were subjected to optimization to minima using the Gaussian16 70 software using the PM6 71 semi-empirical Hamiltonian with cut-off atoms replaced by frozen, dummy atoms. Single-point density functional calculations were carried out using the B3LYP functional [47][48][49][50] and SDD basis set 51  Testing 2-MA on P. mirabilis urease. Antimicrobial growth assay. In vitro susceptibility of compounds, 2-MA and AHA, were determined using microbroth dilution method. Compounds stocks were prepared at 200 mM. Overnight cultures were subcultured (1:1000 into LB). Into a 96-well plate, 100 µL of LB was added to every column except inhibitor alone in column A. Serial dilutions of compounds were prepared (10-100 mM). Additional controls were bacteria minus compound and compound minus bacteria. The plate was incubated with shaking (200 rpm) before measurements overnight (37 °C) and bacterial growth was measured as function of absorbance at 600 nm.
Inhibitor transmembrane movement assay. To access whether compounds could cross the bacterial membrane and inhibit urease intracellularly, the macroscopic changes in pH were measured. To check that the compounds did not alter the viability of the cells, viability cell counts were used to monitor bacteria growth. P. mirabilis and E. coli overnight cultures were prepared. Media pH and CFU/mL were measured at the start (0 min) and the end (90 min) of bacteria growth.
In vitro bladder models and analysis. Bladder models and artificial urine were prepared according to Nzakizwanayo et al. 56 (Tables 2 and 3).
Urea is added to water which has been gently warmed with stirring to allow it to fully dissolve. The 400 mL of solution is filtered through 0.45 µm syringe filter (Millipore, UK). The two solutions were combined with 3.6 L of sterile milliQ water. The pH of the artificial urine was adjusted to 6.1 before use. Compounds were dissolved in urine reservoirs, "kidney" (2 × 5 L, 10 mM per kidney). Models were used to stimulate a late-stage infection, hence they were inoculated with 10 8 CFU/mL of P. mirabilis. Artificial urine containing the compounds were pumped into the bladders at a constant rate of 0.75 mL/min. The number of viable bacteria and pH were measured at periodic intervals throughout. Bladders were treated with 2-MA, AHA, and no treatment.
Crystal violet staining for biofilm analysis. To test whether 2-MA and AHA inhibited biofilm activity, biofilms were prepared as follows. P. mirabilis was subcultured (1:1000 into LB, total volume of 1 mL), each subculture was added to a 12-well micro-plate (Corning, New York, USA) thus the starting inoculum was 10 3 CFU/mL. Microplates were incubated statically with varying concentrations of compound (1, 2.5, 5, 7.5, 10 and 15 mM) for 24 h at 37 °C. To quantify the biofilms crystal violet staining was used, whereby biofilms are washed 3 × with milliQ water removing planktonic cells. The adhered cells were stained with crystal violet solution (0.1% (w/v), 500 μL) and incubated statically for 15 min at RT. The stain was removed with 3 × exhaustive washes with mil-liQ water and dried at RT for 30 min. Quantification of adhered cells occurred by adding decolorizing solution (33% (v/v) acetic acid, 500 μL) to each well and the absorbance measured at 595 nm. Positive control: absence of compound, negative control: absence of bacteria.
Cytotoxicity testing. Testing  www.nature.com/scientificreports/ supplemented with fetal calf serum (10% (v/v), (Merck, Germany)) and cultured in controlled atmosphere (5% CO 2 , 37 °C). Adherent cells were routinely passaged in T75 culture asks every 2-3 days or when cells had reached 70-80% confluency (low passage number cell lines were used for experimental analysis). HaCaT cells were detached by pre-washing in PBS with EDTA (1 mM, 10 mL, incubated for 12 min in order to chelate divalent cations, (Merck, Germany)), followed by trypsinization (0.25%, 3-4 min). Cells were resuspended in fresh medium (DHEM), counted using a hemocytometer, and the cell solution diluted appropriately with DMEM to achieve 5 × 10 3 cells per well. Cells were seeded in triplicate into 96-well plate (200 μL) and incubated overnight to form sub-confluent monolayers. XTT assay: after 24 h incubation, half the incubated media (100 μL) was treated with compounds (sterile filtered 0.22 μm pore size (Millipore) in PBS) at 80, 40, 20, 10, 5, 2.5, 1.25, and 0.625 mM to assess concentration-dependent cytotoxicity. A negative control of PBS was prepared for untreated cells. After overnight incubation with the drug, cells were washed (3 × PBS, 200 μL) to remove residual compound and cell proliferation was measured using a standard 2,3-bis[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5-carboxanilide inner salt (XTT) assay kit (CyQUANT, (Fisher Scientific, UK)). The assay was prepared according to manufacturer's instructions 72 . In brief, XTT-labelling reagent and electron coupling reagent were mixed (ratio 50:1) and added to 96-well plate (50 μL) with fresh DMEM media (100 μL). Plates were incubated for 4 h (37 °C, 5% CO 2 in a humidified atmosphere). Quantification of cell cytotoxicity was measured as a function of absorbance at 450 nm in a plate reader (BMJ Labtech). The cytotoxic effect of treatment was expressed as % viability compared to untreated cells: % cell viability = corrected absorbance of sample cells corrected absorbance of untreated cells x 100 . The background correct was blank (media only). Cells treated with Triton X-100 (20% (v/v) (Merck, Germany)) were used as positive control for cytotoxic effect (treated for the same time as the compounds). Each experiment had three biological repeats.
Ex vivo hemolytic assay. This project has been approved by the University of Bath, Research Ethics Approval Committee for Health (REACH) [reference: EP 18/19 108]. Informal consent was gained from the donor prior to research taking place, and research was carried out following the relevant guidelines defined by REACH. Whole blood (25 mL) was obtained from an anonymous human donor, drawn directly into lithium heparincoated Vacutainer tubes to prevent coagulation. Blood was centrifuged (797g, 10 min, 5810 R Eppendorf, Stevenage, UK), levels of hematocrit and plasma were marked on the tube. Plasma layer was removed, discarded, and hematocrit tube was filled to the volume of plasma with saline solution (150 mM NaCl (Merck, Germany)). Blood cells were washed three times (centrifugation, aspiration of supernatant, and replacement with fresh saline). Pellet containing erythrocytes were resuspended in PBS and divided into 500 μL aliquots. Aliquots were centrifuged (345 g, 10 min, Eppendorf 5418 R, Stevenage, UK), and the supernatant was replaced with 500 μL of varying concentrations (40, 20, 10, 5, 2.5, 1.25, 0.625 mM) of syringe filtered (0.22 μm pore size, Millipore) compounds. Cells were incubated with compounds for 30 min (37 °C, with agitation) before being centrifuged (as before), the supernatant was removed to measure the absorbance of liberated hemoglobin at 415 nm, as described by Tramer et al. 73 . Total lysis of erythrocytes was obtained by treatment with Triton X-100 (20% (v/v)). Degree of hemolysis was expressed as % hemolysis, relative to spontaneous lysis controls.
% haemolysis = corrected absorbance of sample cells corrected absorbance of lysed cells × 100 . Values were background correct to blank (PBS only). Each experiment had three biological replicates.  Table 2. Components 5 × artificial urine, dissolved in 1 L of milliQ water and autoclaved. The pH was adjusted to pH = 5.7 by the addition of sodium hydroxide (all components were purchased from Merck, Germany).