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Figure 3

From: Plasmodium oocysts respond with dormancy to crowding and nutritional stress

Figure 3

Effect of supplemental bloodmeals on oocyst growth. (a) P. berghei oocyst diameter (dia.) in mosquitoes provided no supplemental bloodmeal after the time of infection (non-blood-fed; NBF) or an additional supplemental bloodmeal 7 dpi (BF7) measured 14 dpi. (b) Representative microscopic pictures of P. berghei oocysts in NBF and BF7 mosquitoes taken 14 dpi. (c) PbCyt-b DNA abundance in NBF and BF7 mosquitoes 14 dpi. (d) Correlation between P. berghei oocyst density (log) and PbCyt-b DNA abundance in the midgut of NBF and BF7 mosquitoes (r = 0.35, P < 0.0001). (e) Correlation between P. berghei oocyst density (log) and PbCyt-b DNA abundance PCO. (f, g) Representative microscopic pictures and diameter of P. berghei oocysts on midguts of mosquitoes silenced for LRIM1 and LRIM1 + Lp and maintained under a standard husbandry regime (NBF) or provided a supplemental bloodmeal 7 dpi (BF7). For oocyst diameter, oocysts were randomly selected from 20 midguts from two independent infection experiments. (h) P. falciparum oocyst diameter (dia.) in mosquitoes provided no supplemental bloodmeal after the time of infection (NBF) or an additional supplemental bloodmeal 3 dpi (BF3) measured 9 dpi. (i) Representative microscopic pictures of P. falciparum oocysts in NBF and BF3. (j) PfCyt-b DNA abundance in NBF and BF3 mosquitoes. Horizontal lines in dot plots and boxplots indicate median. Data were generated from at least two independent experiments.

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