Correction to: Scientific Reports https://doi.org/10.1038/s41598-020-71844-8, published online 09 September 2020


This Article contains an error in the Results section, under subheading ‘Apoptotic predisposition of SDS-iPSC–derived hemoangiogenic progenitors.’


“We then investigated the underlying mechanism of the SDS-associated apoptotic predisposition at the hemoangiogenic progenitor stage (Fig. 6a).”


should read:


“We then investigated the underlying mechanism of the SDS-associated apoptotic predisposition at the hemoangiogenic progenitor stage (Fig. 6a). We confirmed that plasmids encoding shRNA against TP53 were not integrated in all SDS-iPSC clones used in Fig. 6.”


In addition, there is an error in the order of the Figures. Figures 2 and 3 are published as Figures 3 and 2 respectively. The correct Figures 2 and 3 appear below as Figures 1 and 2. The Figure legends are correct.

Figure 1
figure 1

Impaired granulopoiesis during in vitro differentiation from SDS-iPSCs. (a) Outline of the defined, step-wise differentiation protocol for generation of mature neutrophils. (b) May–Giemsa staining of floating HCs obtained from SDS-iPSC clones transduced with SBDS or empty vector, and from control iPSCs on day 30 of differentiation. Scale bar: 100 μm. (c) Sequential analysis of the number of floating HCs generated from control SDS-iPSCs, SBDS-overexpressing SDS-iPSCs, and control iPSCs. (d) Sequential analysis of the number of floating HCs generated from SDS-iPSC clones transduced with SBDS or empty vector. (e) Chemotactic activity of floating HCs generated from SDS-iPSC clones transduced with SBDS or empty vector in response to fMLP. fMLP-treated samples were presented as the fold change to untreated control sample. (f) Number of hematopoietic colonies derived from SDS-iPSC clones transduced with SBDS or empty vector, and from control iPSCs. (g) Light micrographs (upper rows) and May–Giemsa staining of hematopoietic colonies (lower rows) generated from SDS-iPSC clones transduced with SBDS or empty vector, and from control iPSCs. Scale bar: 100 μm. Data represent means ± SEM of triplicate wells; representative results from one of three independent experiments are shown (*P < 0.05).

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Figure 2
figure 2

Transduction of SDS-iPSCs. (a) Construction of lentiviral vectors containing DsRed alone (PBCl-EF1a-DsRed-PuroR-pA) or SBDS cDNA and DsRed (PBCl-EF1aSBDS-IRES2-DsRed-PuroR-pA). (b) Western blotting analysis of SBDS protein in SDS-iPSC clones transduced with SBDS or empty (DsRed alone) vector. TF2B was used as a loading control. “ + SBDS” indicates iPSC clones transduced with SBDS vector. (c) Representative polysome profiling of SDS-iPSC clones transduced with SBDS or empty vector. (d) 80S:40S ratio in SDS-iPSC clones transduced with SBDS or empty vector.

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Finally, in the Supplementary Information file, the labels for the original Western blots for Figure 2b are reversed. The correct version of the blots appears below as Figure 3.

Figure 3
figure 3

A correct version of the original Western blots for Figure 2b in the Supplementary Information file.

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