Retrospective analysis of OCT on MB characteristics and 1-year follow-up of the ISR incidence after the DES implantation in patients with MB

We used optical coherence tomography (OCT) to analyze the "half‐moon" like phenomenon and its characteristics and observe 1‐year follow‐up of the in‐stent restenosis (ISR) incidence after the drug eluted stent (DES) implantation in patients with the myocardial bridge (MB). Patients were retrospectively analyzed from January 2013 to December 2019. We used OCT to check 45 patients with MB and found a visible muscle layer (VML) around the vessel adventitia with the same or high density compared to the vessel media layer. There was not any significant difference in maximal thickness, maximal arch, and total length between the half‐moon layer and the visible muscle layer groups (p > 0.05). Maximal thickness, arch, and total length of the half‐moon layer were significantly positively related to VML, respectively (r = 0.962, 0.985, 0.742, p < 0.01). Of these 626 patients with MB seen by OCT, only 300 could be checked out by coronary angiography (CAG). Besides, the larger the thickness and arch of the VML around the vessel adventitia, the more severe the MB in these patients (p < 0.05). After the OCT use, there was no coronary perforation in these patients with MB covered with DES. After 1‐year follow‐up, ISR in MB covered with DES showed a notable difference among no MB, mild MB, moderate MB, and severe MB groups (p < 0.05), and ISR in DES aggravated with the MB severity. However, ISR in MB with and without covered with DES had no significant difference among the 4 groups (p > 0.05). OCT could evaluate MB characteristics accurately compared to IVUS and had a higher rate of detecting MB than CAG. Moreover, it is safe and effective to guide DES covering the mild MB segment in patients with severe coronary lesions detected by the OCT.

There are several methods for the diagnosis of MB, including noninvasive and invasive types 9 . The noninvasive modality for diagnosing MB is cardiac CT, which can easily evaluate the length and thickness of MB in the coronary artery 9,10 . The invasive techniques for diagnosing MB are coronary angiography (CAG) and IVUS (intravascular ultrasound) 9,11 . A significant "milking effect" is present when minimal luminal diameter (MLD) decreases by ≥ 70% during systole and ≥ 35% during mid-to-late diastole 12 . Using IVUS, the characteristic finding is a "half-moon" sign. Only there is an echolucent area between the MB segment and epicardial tissue, which lasts for the whole cardiac cycle 11 . Optical coherence tomography (OCT) is a high-resolution (10-20 μm) intravascular imaging technique that uses near-infrared light. Bose et al. have first reported a male case with chest pain that OCT showed no evidence of atherosclerosis but did show that the vessels were patent in diastole and collapsed in systole 13 . Liu and his colleagues have documented that OCT detected a sharp border and heterogeneous, signal-poor fusiform area indicative of arterial tunneling through the myocardium different from the echolucent muscle layer shown on IVUS in patients with MB 14 . Recently, Liu and his team have revealed that MB is both smaller and thinner than that of the adjacent non-MB segment, explaining why the increased frequency and severity of coronary artery perforation during the stent implantation in the MB segment 15 . However, no data to date reported what the "half-moon" like area shown by the IVUS corresponds to under the OCT detection.
Therefore, in the present study, we first found that the visible muscle layer around the coronary artery adventitia using OCT corresponded to the "half-moon" area using IVUS and then tested the relation between their characteristics in 45 patients with MB detected by both IVUS and OCT. Next, we analyzed the visible muscle layer characteristics in detail according to the MB grading after the CAG evaluation. Finally, we retrospectively studied the clinical significance of OCT for guiding the DES implantation in patients with MB.

Methods
Study design. It was a retrospective study. First, a total of 45 patients with MB received both IVUS and OCT detection. According to the CAG grading 16 , these patients were divided into no MB (n = 25), mild MB (n = 16), and moderate to severe MB (n = 4) groups. Second, 2676 patients were split into no MB (n = 2050) and MB (n = 626) groups after the OCT checking. Third, we further divided 626 patients with MB into no MB (n = 326), mild MB (n = 204), moderate MB (n = 75), and severe MB (n = 21) groups after the CAG evaluation again. Finally, after a 1-year follow-up, the incidence of in-stent restenosis (ISR) was analyzed in these patients. The workflow chart of this study was seen in Fig. 1. All the above-mentioned patients were enrolled from January 2013 to December 2019 in our hospital. The ethics committee of Nanjing First Hospital approved the study protocol, and all methods were performed in accordance with relevant guidelines and regulations. Additionally, written informed consent was obtained from all patients before cardiac catheterization. CAG, percutaneous coronary intervention (PCI), and quantitative coronary angiography (QCA) procedures 17 . Standard techniques performed CAG. Angiographic images were obtained after intracoronary injection of nitrate (100 or 200 mg). All angiograms were analyzed at a core laboratory (Nanjing First Hospital, Nanjing, China) in a blinded fashion. QCA was performed in optimal projections with a validated  www.nature.com/scientificreports/ automated edge-detection coronary system (CAAS version 5.7, Pie Medical Imaging, Netherlands). MLD, reference vessel diameter (RVD), and percent diameter stenosis (%DS) were measured. According to the report of Noble et al., the degree of narrowing of the LAD during systole was graded 3 (greater than 75 percent), graded 2 (50 to 75 percent), and graded 1 (less than 50 percent) 16 . All procedures were performed according to the current PCI guideline. During the procedure, unfractionated heparin was used to maintain activated coagulation time > 250 s. If the procedure time were more than 1 h, an additional 3000 IU of heparin would be added. If aspirin and clopidogrel were not used before admission, it was recommended that all patients took a loading dose of aspirin (300 mg) and clopidogrel (600 mg, or ticagrelor 180 mg) at least 2 h before PCI. The selection of DES type, procedure technique, and the use of glycoprotein IIb/ IIIa inhibitors was at the operator's discretion. The immediate criteria for the successful PCI procedure were that the stenosis lumen was significantly enlarged after the DES implantation or the drug-coated balloon (DCB) therapy. The residual DS was less than 20%, and TIMI blood flow was grade 3. After PCI, all patients were prescribed aspirin 100 mg daily and clopidogrel 75 mg daily (ticagrelor 90 mg twice daily) for at least 12 months. 18,19 . After intracoronary injection of nitroglycerin (100-200 mg), the IVUS catheter was pushed at least 10 mm distal to the lesion or stent edge. IVUS images were obtained through the automatic pullback (0.5 mm/s) by a commercially available imaging system with a 40-MHz mechanical transducer (Boston Scientific, Natick, Massachusetts) for measuring on-site. All IVUS images were stored on a DVD for offline measurements. A "half-moon" like echolucent area surrounding the coronary artery was seen during the whole cardiac diastole and systole cycle. Mark the range of a "half-moon" layer (HML) manually on the cross-section, and the HML's thickness was the difference between long and short radii. Next, choose out the maximal HML thickness. Then, take 4 cross-sections of the HML on average and calculate the mean value of the maximal HML's thickness of these 4 cross-sections for a patient. First, take the vessel center point as the vertex from the cross-section, and the two ends of HML were points A and B respectively. Second, the angle between points A and B was the HML's arch. Finally, take an average of 4 cross-sections to find out the maximal HML's arch for a patient. Find out the starting point and endpoint of HML on both the cross-section and the long axis plane, and calculate the length of HML on the long axis plane. If there is discontinuity, calculate the sum of the lengths of each HML part. The representative case with MB was shown how to measure indexes about the "halfmoon" area ( Fig. 2).

IVUS and OCT procedures
After an intracoronary administration of 1.0 mg nitroglycerin, OCT imaging of the main vessel was serially performed three times (before the intervention, immediately post-intervention, 12 months post-intervention) in all patients using a frequency-domain OCT system (LightLab Imaging, Inc., St. Jude Medical, St. Paul, MN, USA). In this study, an OCT catheter was advanced for imaging at least 10 mm distal to the target lesion. Generation of OCT cross-sectional images was at a rate of 100 frames/s while the fiber optic probe was withdrawn within the stationary protective sheath at a speed of 20 mm/s. All OCT images were analyzed by proprietary offline software (LightLab Imaging or St. Jude Medical) by one independent investigator who was blinded to patient and procedural information. A visible muscle layer (its homogeneous density was greater than or equal to that of the middle layer) around the vessel adventitia was also seen during the whole cardiac cycle. Mark the range of a visible muscle layer (VML) manually on the cross-section, and the VML's thickness was the difference between long and short radii. Next, choose out the maximal VML thickness. Then, take 4 cross-sections of the VML on average and calculate the mean value of the maximal HML's thickness of these 4 cross-sections for a patient. First, take the vessel center point as the vertex from the cross-section, and the two ends of VML were points A and B, respectively. Second, the angle between points A and B was the VML's arch. Finally, take an average of 4 cross-sections to find out the maximal HML's arch for a patient. Find out the starting point and endpoint of VML on both the cross-section and the long axis plane, and calculate the length of VML on the long axis plane. If there is discontinuity, calculate the sum of the lengths of each VML part. The representative case with MB was shown how to measure indexes about the muscle layer ( Fig. 2). Furthermore, we supplied the representative case about the dynamic observation of the MB change detected by OCT through a 1-year follow-up (Fig. 3). The fibrous plaque has high backscattering and a relatively homogeneous OCT signal. Calcified plaque is defined as a plaque with a delineated, signal-poor region with low backscatter. Lipid plaque refers to the plaque with a diffusely bordered, signal-poor region with rapid signal attenuation 20 .
Follow-up procedure and the primary endpoint. We routinely performed clinical follow-up at 1, 6, 9, and 12 months after the DES implantation, and CAG at the same time at the 12th month in our hospital. ISR was the primary endpoint of our retrospective study after 1-year follow-up. It was defined as stent diameter restenosis greater than 50% in CAG 18 .

Statistical analysis. The Kolmogorov-Smirnov test assessed the distribution of continuous variables.
Continuous variables were expressed as mean ± SD for normal distribution and compared using the Student's t-test or described as the median for non-normal distribution and compared using the Mann-Whitney U test. Categorical variables were expressed as frequencies or percentages and compared by chi-square statistics or the Fisher exact test. A p-value < 0.05 was considered statistically significant. All analyses were performed using the statistical program SPSS24.0 (SPSS Institute, Chicago, Illinois).
Ethical approval. The ethics committee of Nanjing First Hospital approved the study protocol, and all methods were performed in accordance with relevant guidelines and regulations. Additionally, written informed consent was obtained from all patients before cardiac catheterization. MB, and moderate to severe MB groups according to the CAG standard for the MB classification. There was not any significant difference in maximal thickness, arch, and total length of the half-moon layer among the three groups (p > 0.05). However, these 3 indexes had a significant increase tendency in the moderate to severe MB group (Table 1). Meanwhile, we also used the OCT to check these 45 patients with MB. We found a visible muscle layer around the vessel adventitia with the same or high density as the vessel media layer. The muscle layer's location detected by the OCT was comparable as the IVUS shows the half-moon layer. Then, maximal thickness, arch, and total length of the muscle layer were measured. These 3 indexes among no MB, mild MB and moderate to severe MB groups showed no significant difference (p > 0.05), but they also had a notable increase tendency in the moderate to severe MB group (Table 1). Next, respective characteristics of the visual signs about MB detected by IVUS and OCT were shown in Table 2. We found that there was not any significant difference in maximal thickness, maximal arch, and total length between the half-moon layer and the visible muscle layer groups (p > 0.05).
Finally, we performed the correlation analysis on these 3 indexes between the half-moon layer (IVUS) and the muscle layer (OCT). Maximal thickness, arch, and total length of the half-moon layer were significantly positively related with those of the visible muscle layer, respectively (r = 0.962, 0.985, 0.742, p < 0.01) (Fig. 4).
These results revealed that a visible muscle layer around the vessel adventitia detected by the OCT could also accurately reflect the MB condition compared to the IVUS.
Basic clinical characteristics between MB and non-MB groups detected by optical coherence tomography. Next, we retrospectively 2676 patients checked with the OCT and found that 626 patients had the phenomenon that a visible muscle layer around the vessel adventitia with the high or same density as the vessel media layer, indicated that these patients had MB in the coronary artery. Between MB and no MB groups, there was not any significant difference in gender, age, hypertension, dyslipidemia, diabetes, and diagnosis [SMI www.nature.com/scientificreports/  www.nature.com/scientificreports/  (Table 3).

Basic clinical and OCT characteristics among no MB, mild MB, moderate MB, and severe MB groups according to coronary angiography. According to CAG, 626 patients with MB detected by
OCT were divided into no MB, mild MB, moderate MB, and severe MB groups. Among these 4 groups, there were significant differences in gender (male), age, hypertension, diabetes, and plaque formation in MB (p < 0.05). However, there were no notable differences in smoking, dyslipidemia, diagnosis, MB continuity, and plaque character (p > 0.05). 23.96% of patients had plaque formation in the MB segment. Among them, 70% of plaques were fibrotic and fatty (Table 4). Maximal thickness, arch, and total length of a visible muscle layer around the vessel adventitia were measured. We found that these indexes mentioned above showed significant differences among these 4 groups (p < 0.05). Furthermore, maximal thickness and the arch of a visible muscle layer increased with the severity of MB, but its total length was not changed like that (Table 4).
These results suggested that the more extensive the thickness and arch of a visible muscle layer around the vessel adventitia, the more severe the myocardial bridge.
PCI strategy and the 1-year follow-up ISR outcome guided by OCT among no MB, mild MB, moderate MB, and severe MB groups according to coronary angiography. According to the current PCI guideline, there was a significant difference in the PCI procedure among the 4 groups (p < 0.01), but PCI strategy (including DES and DCB) showed no significant difference (p > 0.05). The incidence of MB not covered with DES among the 4 groups was markedly high compared to MB covered with DES (p < 0.05). Moreover, the incidence of MB completely covered with DES was notably low compared with MB partially covered with DES  www.nature.com/scientificreports/ (p < 0.01). Under the guidance of OCT, there was no coronary perforation in these patients with MB covered with DES. After 1-year follow-up, there was no significant difference in ISR in MB not covered with DES among the 4 groups (p > 0.05). Besides, ISR in MB with and without covered with DES had no significant difference among these 4 groups (p > 0.05). However, ISR in DES covered with MB showed a notable difference among 4 groups (p < 0.05), and ISR in DES aggravated with the MB severity (Table 5).
These results revealed that it is safe and effective to guide DES covering the mild MB segment in patients with severe coronary lesions after checking with OCT.

Discussion
In the present study, the major findings were as follows: (1) the characteristics of the "half-moon" sign showed by IVUS were almost consistent with a visible muscle layer around the vessel adventitia with the same or high density as the vessel media layer in patients with MB. (2) the detection rate of MB in OCT was significantly high compared to CAG, and LAD was the most location of MB. (3) the larger the thickness and arch of a visible muscle layer around the vessel adventitia, the more severe the MB in patients checked with OCT. (4) under the guidance of OCT, it was safe and effective to use DES to cover the mild MB in patients with severe lesion after 1-year follow-up.
MB is characterized by epicardial coronary artery tunneling through the myocardium, with the angiographic "milking" phenomenon and a "half-moon" echolucent detected by IVUS 9,10 . The IVUS hallmark of a tunneled segment of the coronary artery is a variable degree of compression that persists into diastole, with the typical finding of the "half-moon phenomenon", an echolucent area present only between the bridged coronary segment and epicardial tissue in the whole cardiac cycle 9,10 . However, the etiology of this phenomenon is not clear. OCT is a novel imaging technology based on light, which can be used to study tissues in vivo 20 . Its ultra-high resolution is about 10 µm, which can display the anatomical structure of the coronary artery and provide a high-quality coronary artery image 20 . Xu and his team have reported that the length of the MB measured by OCT appeared much longer than that measured by CAG, and the maximal extent of MB stenosis appeared significantly small with OCT compared to CAG, indicating that OCT can more clearlyobserve the morphology and intimal structure of MB than CAG 21 . Recently, Liu and his colleagues have revealed that the coronary artery involved in MB Table 3. Basic clinical characteristics between MB and non-MB groups detected by optical coherence tomography. Data were expressed as n (%), mean ± SD. Variance analysis, P1; SMI silent myocardial ischemia, SA stable angina, UA unstable angina, AMI acute myocardial infarction, LAD left anterior descending coronary artery, LCX left circumflex coronary artery, RCA right coronary artery, CAG coronary angiography. www.nature.com/scientificreports/ www.nature.com/scientificreports/ was smaller and thinner than that of the adjacent non-MB segment 15 . However, no data to date reported what a "half-moon" echolucent in IVUS corresponds to after the OCT checking. After retrospectively analyzing 45 patients with MB after IVUS and OCT detection, we found that the characteristics of the half-moon sign in IVUS were highly related to the visible muscle layer around the vessel adventitia in OCT, which had the same or high density as the vessel media layer in patients with MB. As we knew, the sample size of patients with MB checked with IVUS and OCT was more than ever reported. It has revealed that the larger the thickness and radian of the "half-moon" like area was, the more serious it was in patients with MB after the IVUS checking 7,9,10 . Therefore, with more severe MB, the thickness and radian of the visible muscle layer around the vessel adventitia became more significant detected by OCT in our study.
Although CAG might seem a valuable modality for anatomic assessment, the sensitivity of CAG for the MB detection is low, generally estimated as about 5%, ranging from 0.5% and 12% in previous studies 9,10,12 . However, IVUS is usually considered as a more specific and invasive modality for the anatomic detection of MB 9,10 . During the whole cardiac cycle, IVUS presents as an echolucent "half-moon" area adjacent to the lumen 9,10 . In our study, the characteristics of the visible muscle layer around the vessel adventitia detected by OCT were highly related to an echolucent "half-moon" area checked by IVUS. Thus, the sensitivity of OCT for detecting MB was also significantly high compared to CAG. MB can be found in any epicardial artery, and most of them (70-98%) involve the LAD 22,23 . However, in the study, LAD was the only coronary artery with the MB segment by the OCT detection.
Small sample size, single-center data, and retrospective study might explain why the above phenomenon happened. MBs are mostly found in the middle segment of the LAD, and the result was similar to our finding. Unlike CAG, OCT could not only identify MB but also the plaque character 20 . Xu and his colleges have reported that stable fibrous plaques, but no lipid or other types of plaque, were found in the MB segment of all 12 patients under the guidance of OCT 21 . However, the sample size is too small. Besides the fibrous plaque, lipid, fibrous and calcified, and fibrous and lipid plaques were found in the MB segment of our study. Among them, the most common was the fibrous and lipid plaque. The MB segment is always free of atherosclerosis 24 . Midiri and his team have reported that employing computed tomography coronary angiography (CT-CA), 9.4% of patients presented the atherosclerotic lesion in the MB segment 24 . It is sensitive to use invasive coronary catheters such as OCT and IVUS to detect MB compared to CT-CA 9,10 . Therefore, in the present study, 24.0% of patients had an atherosclerotic lesion in the MB segment.
Stent into the MB segment could lead to a high rate of coronary artery perforation, stent restenosis, and stent fracture, etc. [25][26][27] . However, there are some conditions where a stent is required to wholly or partially cover the MB. For example, severe plaque burden results in serious narrowing in the MB segment, or there is no good distal landing zone for stent implantation 26,27 . In the present study, under the guidance of OCT, there was no perforation incidence in the MB segment wholly or partially covered with DES, revealing that covering the MB guided by OCT was a safe procedure. After a 1-year follow-up, the ISR incidence in MB with the DES coverage was comparable with MB without the DES coverage among no MB, mild MB, moderate MB, and severe MB groups. Additionally, the high incidence of in-stent restenosis is accompanied by the increased severity of MB. These results were indicated that it was safe and effective to use the DES wholly or partially cover the mild MB segment detected by the OCT.

Limitations
It was a retrospective and single-center study. Furthermore, the sample size was relatively small.

Conclusion
OCT could accurately evaluate the characteristics of a visible muscle layer around the vessel adventitia in patients with MB compared to IVUS. The MB detective rate of OCT was significantly high compared to CAG. Finally, covering the mild MB segment with DES was relatively safe and effective in patients with a severe coronary lesion.

Data availability
The data that support the findings of this study are available from the corresponding author upon reasonable request. www.nature.com/scientificreports/