Plant canopy may promote seed dispersal by wind

Seed dispersal has received much research attention. The plant canopy can intercept diaspores, but the effect of the plant canopy (the aboveground portion of a plant consisting of branches and leaves) on dispersal distance has not been explored empirically. To determine the effect of plant canopy on seed dispersal distance, a comparison of diaspores falling through open air and through plant canopy was made in a wind tunnel using three wind speeds and diaspores with various traits. Compared with diaspores falling through open air, the dispersal distance of diaspores falling through plant canopy was decreased or increased, depending on wind speed and diaspore traits. When falling through a plant canopy, dispersal distance of diaspores with thorns or those without appendages was promoted at low wind speed (2 m s−1), while that of diaspores with low wing loading (0.5 mg mm−2) and terminal velocity (2.5 m s−1) was promoted by relatively high (6 m s−1) wind speed. A plant canopy could increase seed dispersal distance, which may be due to the complicated updraft generated by canopy. The effect of maternal plants on seed dispersal regulates the distribution pattern and the species composition of the community.

www.nature.com/scientificreports/ field studies indicated that shrub canopy can intercept diaspores with appendages (hair, samara, wing, balloon) during low wind speeds 29 , however, the effect of seeds passing through the canopy on their dispersal distance is still unclear [30][31][32][33][34] . Pounden et al. 35 found that winged diaspores had significantly reduced dispersal following boles collision, the hair diaspores did not, but how the plant canopy (i.e. aboveground portion of a plant consisting of branches and leaves) affects other types of diaspores is still unknown 36,37 . So, we hypothesized that the plant canopy can reduce diaspore dispersal distance, which is controlled by diaspore traits and wind speed. To test our hypothesis, controlled experiments were conducted in a wind tunnel, using 29 species with different diaspore traits.

Materials and methods
Diaspore selection and trait measurements. To determine the relationship between diaspore traits and dispersal distance of diaspores passing through plant canopy versus through open air at different wind speeds, we selected diaspores of 29 plant species that differ in appendage type, mass, projected area, shape index, wing loading, and terminal velocity (Table 1), differences in traits of the selected species were not restricted by phylogeny, and each diaspore was only considered as a representation of its own morphological attributes. Firstly, diaspores with different types of appendages (samara, wing, thorn, hair and balloon) were selected, and then the gradient of the same appendage diaspores was set according to the quality. To facilitate seed dispersal investigation, diaspores with samaras, wings, thorns, and without appendage were lightly sprayed with red aerosol paint, while diaspores with hairs were colored using red water-based markers. The dyed diaspores were naturally air-dried and placed in plastic boxes to ensure the integrity of morphological structure. Diaspore traits are measured after dyed and dried.
Twenty intact diaspores of each type were selected the same species for measurements of length, width, and thickness of each diaspore were measured with Vernier caliper (0.01 mm accuracy). Diaspores shape index (Vs) was calculated using an equation we developed from ideas in Thompson et al. (1993).
Length , x 2 = Width Length , and x 3 = Height Length . Diaspore mass was determined using an electronic balance (0.0001 g), mass range of 29 diaspores were 1.12-316 mg. The projected area of diaspore was scanned with a digital scanner, and measured with analysis software (Motic Image Plus 2.0, Motic China Group Co., Xiamen, China), and project area range of 29 diaspores were 5-604 mm 2 . The wing loading was calculated as seed mass divided by projected area 38,39 , and wing loading range of 29 diaspores were 0.04-2.1 mg mm −2 . Terminal velocity was measured with a camera described by Zhou et al. (2020), and terminal velocity range of 29 diaspores were 0.7-40 m s −1 ( Table 2).
Wind speed control. A wind tunnel (with a test section 2 m high × 2 m wide × 20 m long) was used to control wind speed. Wind speed was monitored inside the tunnel with a Pitot tube (160-96, Dwyer Instruments, Inc., Indiana, USA) connected with a Magnesense II Differential Pressure Transmitter (MS2-W102-LCD, Dwyer Instruments Inc., Indiana, USA) (Liang et al. 2020). In this study, wind speeds (measured 1 m above the flat sand surface) were set at 2, 4 and 6 m s −1 , and they correspond to categories 3-5 of meteorological wind measurements, which is basically close to the common range of wind speeds under natural conditions (Mather, 1987). The underlying surface is set with a flat fixed sand surface in this experiment.
Model plant canopy setting. A young tree (sapling) of Ulmus parvifolia was selected for use as the model canopy because it has a high density of branches and many leaves. Further, this species is relatively easy to transplant and tolerant of high wind speed. Plant height was 1 m (Fig. 1), all leaves were present, and size of canopy was 1.0 m high × 1.2 m wide. The model plant selected was only used to explore the effect of canopy on seed www.nature.com/scientificreports/ dispersal distance and was not related to the maternal plants of the experimental diaspores. The model sapling was transplanted (transplanted with roots to ensure plant survival, and the root part is fixed with sand to keep the underlying surface flat) from the field to the front end of the wind tunnel test section,8 m away from the power section and in the middle of the two walls of the wind tunnel. The model plant grew well throughout the experiment.

Diaspore release.
To standardize our studies of the dispersal of diaspores through the canopy, we set the diaspore release point as the center of the canopy, 10 cm below the top of the canopy and 90 cm from the soil surface. A 4 cm diameter stainless steel tube was used as the release device. The release device was inserted into the canopy from the top of the wind tunnel. Diaspores were released from the upper part of the steel tube (which was controlled by a bottom flap to ensure that initial release rate was zero) when the target wind speed was reached. Five replicates of 20 diaspores of each type were used for each wind speed.
Effect of wind speed on diaspore dispersal through plant canopy. Five replicates of 20 diaspores of each type were used for each wind speed . The 20 diaspores were released simultaneously from the release device through the plant canopy, when the target wind speed was reached (2, 4 or 6 m s −1 ). We turned off the wind tunnel after 2 s of diaspore dispersal. The horizontal (dispersal) distance that each of the 20 diaspores was moved from the release point was measured. Mean dispersal distance was used to represent dispersal ability.
The control was the release of diaspores at a height of 90 cm from the ground through air at the three wind speeds, i.e. after the plant was removed from the wind tunnel. Diaspore dispersal distance was measured after the wind speed was maintained for 2 s. Statistical analysis. The difference in the dispersal distance of each type of diaspore passing through the canopy versus through open air at different wind speeds was compared. Redundancy analyses (RDA) based on correlation matrices of diaspore dispersal distance and explanatory factors were conducted using Canoco 5.0 www.nature.com/scientificreports/

Results
Influence of plant canopy on diaspore dispersal distance at different wind speeds. Plant canopy either increased or decreased diaspore dispersal distance, depending on diaspore traits and wind speed. Compared with dispersal through open air, dispersal distance of nine diaspore types (1 samara (of 7 tested), 3 thorn (of 5 tested), 4 without an appendage (of 7 tested), and 1 balloon (of 2 tested)) was significantly promoted by the plant canopy at a wind speed of 2 m s −1 . Dispersal distance of diaspores with a thorn or those with no appendage was more likely to be promoted by the plant canopy than that of the other kinds of diaspores at a wind speed of 2 m s −1 . However, dispersal distance of diaspores with a wing and those with hair was decreased by the plant canopy. The dispersal distance of all types of diaspore passed through the canopy were not significantly increased compared with that of diaspore passed through the open air when the wind speed was 4 m s −1 . Dispersal distance of six diaspore types (3 samara (of 7 tested), 1 wing (of 7 tested), 1 hair (of 4 tested), and 1 balloon (of 2 tested)) was significantly increased by the plant canopy at a wind speed of 6 m s −1 , and dispersal distance of diaspores with a thorn or those with no appendage was decreased by the plant canopy. At a wind speed of 6 m s −1 , dispersal of diaspores with a wing was increased more by the canopy than that of the other kind of diaspores, and dispersal distance for diaspores without an appendage was significantly decreased (Fig. 2).
Influence of diaspore traits on dispersal distance by wind. The contribution (percentage) of diaspore traits to dispersal distance increased with wind speed (Table 3). Wing loading and terminal velocity had negatively correlations with diaspore dispersal distance for diaspores released through open air and through the plant canopy at wind speeds of 4 and 6 m s −1 , and wing loading was more important than terminal velocity www.nature.com/scientificreports/ for dispersal distance. However, wing loading and terminal velocity did not have a significant effect on diaspore dispersal distance through the plant canopy at a wind speed of 2 m s −1 . Neither shape index, projected area nor mass had a significant effect on diaspore dispersal distance either through the plant canopy or through open air at any of the three wind speeds. At a wind speed of 6 m s −1 , the plant canopy increased dispersal-distance of diaspores with a small wing loading (≤ 0.5 mg mm −2 ) and terminal velocity (≤ 2.5 m s −1 ), and samaras had the greatest dispersal distance followed by diaspores with wing, hair, and balloon. The plant canopy decreased dispersal distance of diaspores with high wing loading and high terminal velocity (Fig. 3).  www.nature.com/scientificreports/

Discussion
Our study indicated that compared to dispersal through open air, diaspores with thorns or without an appendage were more strongly deflected by the canopy at low wind speed, thus their dispersal distance was also more likely to increase. Previous study found that the plant canopy had little effect on interception of diaspores with thorns and without appendages 29 , but it will disperse farther after passing through the canopy at low wind speed. Other diaspores (samara, hair, wing, and balloon) will be more easily intercepted by plant canopy at low wind speed 29 , and thus their dispersal distance was more likely to be decreased. Our study showed that the dispersal distance of samara diaspores were more easily increased by plant canopy at a higher wind speed than dispersal through air (Fig. 2), this may because samara diaspores were subjected to a strong updraft, The effect of wind on seed dispersal distance is complex in nature, resulting in different vertical and horizontal components of air flow including turbulence 10,40,41 , and complex updrafts potentially increasing wind dispersal within a canopy 42 . The promotion of dispersal distance of some diaspores falling through the plant canopy (Fig. 3) is contrary to the branch interception of diaspores 43,44 . Increased dispersal distance may due to induction of an updraft by wind hitting the plant canopy or by change in direction of diaspores that collide with a plant part can change the original dispersal trajectory and increase dispersal distance. Wind conditions can be altered when wind passes through a plant canopy, such as by the generation of an updraft, increasing of vertical wind speed, and reducing of horizontal speed 39,45,46 . Low horizontal wind speed will decrease diaspore dispersal distance, but an updraft or vertical wind speed can increase dispersal time, thereby increasing diaspore dispersal distance. Thus, the plant canopy can increase or decrease diaspore dispersal distance compared to diaspores passing through open air at the same wind speed.
Tackenberg et al. 47 found that updrafts are important for long-dispersal of dandelion in an open meadow environment. Our results showed that plant canopy not only promoted the dispersal of hairy diaspores, but also promoted the dispersal of diaspores with samara, wing, and balloon, which might be because of localised updrafts  www.nature.com/scientificreports/ and eddies caused by plant canopy. Variety of canopy types will cause differences in turbulence, which may further affect the distance of seed wind dispersal. The canopy of branch and leaf density may produce complex updrafts, therefore, we speculate that a dense canopy of branches and leaves is more likely to promote seed wind dispersal, and wind-borne seeds are easier to dispersal over long distances in forests than in deserts, of course, this needs further research to confirm. Diaspore dispersal distance is strongly correlated with wing loading and terminal velocity 3,48,49 . Our study indicated that the dispersal distance of diaspores with small wing loading and terminal velocity can be promoted by the plant canopy at high wind speed but not at a low wind speed (Fig. 3). This is because diaspores with low wing loading and terminal velocity are easily accelerated laterally by the drag of the wind following a collision with a solid object during high wind speed. Diaspores that are dispersed by wind generally have a smaller terminal velocity and wing loading, this means that wind-borne species are distributed more widely in vegetationcovered areas than in open landscapes.
In conclusion, a plant canopy may increase dispersal of diaspores with thorns or those without appendages during low wind speed, and dispersal of diaspores with small wing loading and terminal velocity at high wind speeds. An estimation of the plant canopy effect on diaspore dispersal distance can improve the accuracy of the assessment of dispersal distance of a cohort of diaspores in the real world. Accordingly to our results, we can predict that diaspores with small terminal velocity and wing loading are easier to dispersal long distances under hurricane or storm conditions.

Data availability
The data presented in this study are available on request from the corresponding author.