Abstract
In this research, dispersion of a new type of one-dimensional inorganic material Nb2Se9, composed of van der Waals bonds, in aqueous solution for bio-application study were studied. To disperse Nb2Se9, which exhibits hydrophobic properties in water, experiments were carried out using a block copolymer (poloxamer) as a dispersant. It was confirmed that PPO, the hydrophobic portion of Poloxamer, was adsorbed onto the surface of Nb2Se9, and PEO, the hydrophilic portion, induced steric hinderance to disperse Nb2Se9 to a size of 10 nm or less. To confirm the adaptability of muscle cells C2C12 to the dispersed Nb2Se9 using poloxamer 188 as dispersant, a MTT assay and a live/dead assay were performed, demonstrating improvement in the viability and proliferation of C2C12 cells.
Similar content being viewed by others
The development of nanotechnology has led to the study of a variety of inorganic nanomaterials and has been used extensively for the study of low-dimensional materials (e.g., nano-thick two-dimensional (2D) materials such as graphene and one-dimensional (1D) linear materials such as nanowires)1. Based on this, nanomaterials have begun to be used in various applications such as electronic devices2, optical devices3, catalysts4, environment technology5, and biotechnology6. In particular, bio-applications have been utilized in various fields in in-vitro (e.g., electrical and optical bio sensors7,8) as well as in-vivo (e.g., plasmonic photothermal therapy9, magnetic resonance imaging10) environments based on the characteristics of various inorganic materials, such as superior durability, and electromagnetic and optical properties that cannot be found in the organic materials constituting the existing living body.
However, as the size of existing nanomaterials decreases, the ratio of surface dangling bonds to the nanomaterial size increases, and the characteristics of the material properties expected in the bulk state are degraded (e.g., decrease in electron mobility, optical damping, change in magnetic susceptibility)11. To solve these problems, 2D nanomaterials based on van der Waals (vdW) bonding without surface dangling bonds have been studied. However, the 2D material is a plate-like structure, which still contains dangling bonds at the edges, and there are structural limitations to having various sizes and shapes for bio-application12. Therefore, to apply inorganic nanomaterials to the biotechnology field, novel materials are needed; accordingly, 1D vdW materials have started to receive much attention in recent years.
There are two types of 1D vdW structures; a true 1D structure (e.g., hexagonal tellurium13, Mo6SxI9-x14, VS415, Nb2Se916, Ta2Pd3X817 (X = S, Se), Sb2Se318,19, and V2Se920) with a pure vdW inter-chain bond and a quasi-1D structure (e.g., transition metal trichalcogenides (TiS321, TaSe322, ZrSe323, NbSe324, etc.), Nb2PdS525, and CsBi4Te626) with additional non-vdW bonds among molecular chains. In the case of the quasi-1D structure, it is difficult to separate molecular chains that contain bonds other than the vdW variety and control their size and shape. However, in the case of the true 1D structure, it is possible to fabricate an atomic-scale inorganic nanowire without dangling bonds through mechanical separation27,28,29 or chemical dispersion29,30,31. In our research group, we have synthesized a large number of true 1D materials and published the results of separating them into atomic units20,31. These materials have excellent surface stability because they have no surface dangling bonds, and their nanostructures of various shapes and sizes are easy to fabricate. Thus, they are likely to be applied to biotechnologies, such as extracellular matrix fabrication29, tissue engineering32, and drug delivery33. To apply this true 1D material to the biotechnology field, first, aqueous dispersion must be performed, and the dispersed material should be biocompatible with no toxicity34. However, it is known that the 1D material surface composed of vdW bonds has a very small dipole moment, owing to its nature, and a hydrophobic surface; thus, aqueous dispersion is very difficult. Therefore, it is very important to design a dispersant suitable for aqueous dispersion.
In this study, we used poloxamers as a dispersing agent to disperse the new types of true-1D Nb2Se9 (see Fig. 1a for crystal structure), which can be mass-synthesized at the cm-scale by a flux method. Nb2Se9 is composed of strong intra-chain bonds and weak inter-chain interactions of molecular unit chains. Poloxamers are the ABA (PEO-PPO-PEO) type block-copolymer. ABA triblock copolymer has a typical dispersant structure as a polymer having a portion B adsorbed on the surface of a nanomaterial and a portion A that provides affinity with a solvent. They exhibit good hydrophilicity through PEO at both ends and can be dissolved in water. PPO in the middle can be hydrophobic and adsorbed onto the hydrophobic surface of Nb2Se9. That is, the dispersants can be adsorbed through the hydrophobic-phobic interaction between PPO and Nb2Se9, and the dispersion can proceed through the steric hinderance of the PEO tail (see the schematic in Fig. 1b). Furthermore, ether groups such as PEO and PPO have excellent biocompatibility. First the effect of Poloxamer structure was investigated for dispersing Nb2Se9 in deionized water, and PBS buffer solution, similar environment with real biological environment. The morphology and chemical state of exfoliated Nb2Se9 was characterized by AFM, TEM and XPS. Furthermore, biocompatibility of Nb2Se9 dispersed with poloxamer 188 was verified using the MTT assay and the proliferation test of C2C12 cells. Biocompatible and water dispersible Nb2Se9 has the great potential as a building block to make biomaterials for drug delivery, bioimaging, and theragnostic.
Result/discussion
In order to study the effect of poloxamer structure, six poloxamers having different numbers of PO and EO chains was adapted (see the Fig. S1). Optical measurements (UV–Vis absorption and Tyndall effect) were performed to verify the aqueous dispersion performance of Nb2Se9 using poloxamers. First, starting material, Nb2Se9 crystal is immersed in the water with poloxamer and ultrasonication is carried out on it. Centrifugation removes the undispersed portion and the laser irradiation reveals a pronounced Tyndall effect and increased UV–Vis absorption. When Poloxamer was not used, the Nb2Se9 was not dispersed and sunk to the bottom of the water. However, when the poloxamer dispersant is used, uniform dispersion proceeds without residue (see the strong Tyndall effect in the Fig. 2a and Fig. S2). In deionized water, the dispersion of Nb2Se9 nanowire dispersed by various poloxamers was stable after 1 week.
To compare the dispersibility using various poloxamers, the intensity of the characteristic UV–Vis absorption peak near 400 nm in the solutions after centrifugation was plotted (see the Fig. 2b,d) Well-dispersed Nb2Se9 nanowires have a broad UV–Vis absorption at 350–650 nm and shoulder peaks at 400 and 570 nm35(see the Fig. S3 and S5). As shown in Fig. 2b, the aqueous dispersion of Nb2Se9 was stable using the poloxamers with molecular weight of approximately 3000. When the molecular weight is too small, the steric hinderance was not enough to stabilize the Nb2Se9 wires in water due to its short PEO chain. In addition, when the molecular weight is too large, the poloxamer cannot be adsorbed effectively on the surface of Nb2Se9 chains.
In PBS solution, the improvement in dispersion of Nb2Se9 through poloxamer is more pronounced than in pure water. In the PBS solution without the poloxamer, Nb2Se9 was hardly dispersed and mostly sunken, while the dispersibility was clearly improved with the poloxamer (Fig. 2c and Fig. S4). In PBS buffer solution, the dispersion stability of Nb2Se9 is improved as the molecular weight and the ratio of PEO chain increased (Fig. 2d). Nb2Se9 nanowires dispersed with poloxamer 101, 105, 181, and 184 whose molecular weight is less than 3,000 were hardly dispersed, showing very weak Tyndall effect and low UV–Vis absorption at 400 nm. On the other hand, the Nb2Se9 dispersed solution dispersed with poloxamers 188 and 403 having a molecular weight of more than 5,000 showed strong Tyndall effect and UV–Vis absorption at 400 nm. The solution dispersed with poloxamer 188 with a longer hydrophilic PEO tail was able to disperse Nb2Se9 at a higher concentration than the solution with poloxamer 403, and both dispersions with poloxamer 188 and 403 were stable for 1 week. This is due to the compression of electrical double layer by present ions in the buffer solution. As the electrical double layer is compressed, stronger steric hinderance is needed to stabilize the dispersion of Nb2Se9 in the PBS solution. Therefore, the poloxamers which have more PEO chains and large molecular weight such as poloxamer 188 and 403 were effective. In the zeta potential analysis of the dispersed solution, short poloxamers such as 101, 105, 181, and 184 had a zeta potential of − 30 to − 35 mV because they could hardly screen the surface charge (− 37 mV) of Nb2Se9 (see the Fig. S6). On the other hand, the long poloxamers such as 188 and 403 screened the surface charge of Nb2Se9 and had a zeta potential of − 20 mV similar to the surface charge of ethylene oxide. Even though the electrostatic charge was smaller in the dispersion solution using the long poloxamer, the result of showing more excellent dispersibility is that the effect of steric repulsion was greater than that of electrostatic repulsion on the dispersion of Nb2Se9 in the electrolyte. Improving the dispersibility of Nb2Se9 through the poloxamer 188 on PBS solution facilitates its more effective utilization in the real-life environment. Accordingly, the Nb2Se9 dispersed with poloxamer 188, which showed the best dispersibility in the electrolyte, was applied for further analysis.
The enhanced dispersibility of Nb2Se9 through the poloxamer 188 is due to the hydrophobic-phobic interaction between the PPO chains and Nb2Se9 surfaces, and the steric hinderance of PEO chains. That is, to confirm the phenomenon of PPO physisorption on the surface of Nb2Se9, the XPS analysis of Nb2Se9 surface dispersed by the poloxamer 188 was performed. As can be seen from the core level spectra of Nb 3d and Se 3d (Fig. 3a,b), there was no change in the peak shape or binding energy according to the use of poloxamer 188 dispersant (If the electron is moved from the poloxamer 188 to Nb2Se9, the lower binding energy shift, or from Nb2Se9 to the poloxamer 188, higher binding energy shift)35. This indicates that electron transfer and new compound formation reaction did not occur between the PPO of poloxamer 188 and the Nb2Se9 surface; rather, only physisorption occurs.
The size of Nb2Se9 dispersed by poloxamer 188 was analyzed by AFM (See the Fig. 4a and 4b). It can be confirmed that the 1D-structured Nb2Se9 chains with an average diameter of ~ 10 nm were well dispersed (minimum diameter was ~ 6 nm). Owing to large molecular weight of poloxamer 188 molecules, the dispersant is difficult to remove completely, resulting in residue in the AFM images (this phenomenon is common in other previous studies using polymer dispersants)36. The 10-nm-thick dispersed Nb2Se9 nanowires are shown in the TEM images in Fig. 4c and 4d (residue of polymer dispersant can also be seen).
For the muscle tissue cells such as C2C12, the nanofibrous structure of extracellular matrix (ECM), which the Nb2Se9 nanostructures dispersed by the poloxamer 188 would mimic, is the most important issue. To evaluate the cytotoxicity of Nb2Se9, the MTT assay was performed (Fig. 5a). Although low viability is demonstrated as the treated concentration of Nb2Se9 on C2C12 is increased, it was recovered very quickly, within 24 h, owing to the interaction of C2C12 with the Nb2Se9 nanofibrous structure, which resembles its own ECM. For the long-term cytotoxicity, live/dead assay was also performed (Fig. 5b). The nanofibrous Nb2Se9-coated PDMS was compared with bare PDMS (FDA approved material with sufficient biocompatibility to be implanted in human body) control in this experiment. After 9 d, the proliferation of C2C12 on Nb2Se9-coated PDMS is enhanced compared with bare PDMS, indicating a greater filling area in the live/dead assay. Thus, the nontoxicity of the Nb2Se9 in the C2C12 cell line is confirmed by both the MTT and Live/Dead assay. To check the long-term biocompatibility of Nb2Se9 on C2C12, the MTT assay was selected (Fig. 5c). MTT assay performed on PDMS and the Nb2Se9-coated PDMS in the same way as the Live/Dead assay. The cell proliferation of C2C12 was normalized by day 1 for each PDMS film (bare and Nb2Se9-coated PDMS). The Nb2Se9-coated PDMS shows a growth of 354.5 ± 47.91% in 168 h of culturing (linear increase with time), compared with 195.3 ± 22.63% for bare PDMS (even lower than in 72 h of culturing). These results explain that the nanofibrous Nb2Se9-coated film provides good conditions for C2C12 cell growth.
Conclusion
In conclusion, Nb2Se9, a new 1D material based on vdW binding, was dispersed in water and applied to the ECM of muscle cells C2C12 for biotechnology applications. To disperse hydrophobic Nb2Se9 in water and PBS solution, poloxamers (a block copolymer in which PEO-PPO-PEO units are polymerized in an ABA structure) were used as a dispersant. It was confirmed that PPO, the hydrophobic portion of poloxamer, was adsorbed onto the surface of Nb2Se9, and PEO, the hydrophilic portion, induced steric hinderance to disperse Nb2Se9 to a size of 10 nm or less. Both an MTT assay and a live/dead cell assay were performed to evaluate the viability and proliferation of C2C12 in Nb2Se9 dispersed using poloxamer 188. The Nb2Se9-coated PDMS shows a growth of 354.5 ± 47.91% in 168 h of culturing, compared with 195.3 ± 22.63% for control-PDMS. It has been demonstrated that these vdW 1D materials can be used for various bio-related applications if the dispersion in water is efficient.
Methods
Preparation of Nb2Se9 crystal
The preparation of Nb2Se9 was prepared as previous report37. Single-crystalline Nb2Se9 was synthesized using the flux method in an evacuated quartz tube. The pelletized 0.2 g of Nb (325 mesh, 99.5%, Sigma-Aldrich) and 34 g of Se (99 + %, Alfa Aesar) mixture in a molar ratio of Nb:Se = 1:200 was sealed in an evacuated quartz tube in a 15-cm-long quartz tube with a neck in the middle of the tube. The quartz ampoule was heated for 3 days at a temperature of 700 °C and then cooled. After cooling naturally, the unreacted Se was roughly separated by turning the quartz tube over and dropping the Se liquid to the bottom side of the tube at 250 °C. The resulting material has a dark gray needle shape. The unreacted Se was completely sublimated by heat treatment in a tube furnace at 250 °C under Ar atmosphere for 24 h.
Preparation of aqueous dispersed Nb2Se9
To obtain aqueous dispersion, various types of triblock copolymer with different length of PEO and PPO chain, poloxamers (Sigma-Aldrich) were used as a dispersant. First, 10 mg of dispersant, poloxamer, was dissolved in 10 mL of deionized water or 1X PBS solution. After immersing 10 mg of single-crystalline Nb2Se9 in the solution, ultrasonication was performed by the probe sonication (VC 505, Sonics & Materials, Inc.) at intervals of 2 s on / 2 s off for 5 min, followed by 3 h of bath sonication (B2005S-68 K, 68 kHz, 200 W, KODO Technical)35. After sonication, the solution was centrifuged at 6000 rpm for 10 min to remove any insufficiently exfoliated chains, and 5 ml of the supernatant was used for further analysis.
Characterization
To compare the concentration of dispersed solutions, their UV absorption were characterized by UV–Vis spectrophotometer (Agilent Technologies lnc., Agilent 89090A). The zeta potential of dispersed solution was investigated by Zetasizer (Malvern Instruments Ltd., Nano-ZS90). The chemical state of the Nb2Se9 was examined by XPS (Thermo, ESCALAB250). The samples for XPS were prepared by vacuum filtration method on the AAO membrane with a pore diameter of 100 nm. The morphologies of the exfoliated nanowires were analyzed by atomic force microscopy (Park Systems, NX 10) and aberration-corrected scanning transmission electron microscopy (STEM, JEOL, JEM-2011F). The AFM was operated in non-contact mode, and the samples for AFM were prepared on SiO2/Si wafers by spin-coating. The samples for STEM were prepared by drop-casting onto a carbon-coated TEM grid35.
Cell lines
The C2C12 cell line was obtained from the Korean Cell Line Bank (KCLB, Seoul, Republic of Korea). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, PAN biotech), supplemented with 10% fetal bovine serum (JCBIO) and 1% penicillin–streptomycin (PAN biotech). The cells were incubated at 37 °C in a standard atmosphere (95% air and 5% CO2) during all experiments. The cell culture was maintained with 9 × 105 cells in 75-cm2 cell culture flasks (SPL Life Sciences), and sub-cultured every 48 h after seeding by replacing the culturing media every day. The cell was cultured for three passages after thawing to stabilize the growth conditions29.
Proliferation of C2C12 with Nb2Se9 dispersed by poloxamer 188
The 5 × 103 cells were seeded 24 h before the experiment for normalization in each well of a 96-well plate (SPL Life Sciences). The solution of Nb2Se9 dispersed by poloxamer 188 (Nb2Se9-188) was prepared in 1X PBS (Gibco), and subsequently, a UV-sterilized procedure was conducted before mixing with the complete media. The dispersed solution was condensed to the required concentration using an Amicon filter. The Nb2Se9-188 was mixed with complete media (1:9 vol%) to obtain the final concentration, and 200 μL of the mixed media was treated to C2C12 in each 96-well plate. After the required incubation time passed, 0.5 mg/mL of filtered thiazolyl blue tetrazolium bromide (MTT) in serum-free media was replaced with previous culturing media in a 96-well plate. C2C12 with the MTT solution was incubated for 3 h in 37 °C and replaced with dimethyl sulfoxide (DMSO) for the lysis and dissolution of formed formazan. A spectrophotometer (Varioskan LUX) was used to obtain the absorbance of 570 nm and 630 nm of the samples. The proliferation was calculated by subtracting the absorbance of 630 nm from 570 nm in each sample29.
Substrate fabrication
The substrate and wells of the PDMS well plate were fabricated using SYLGARD 184 Base & Curing Agent Kit (Dow Corning). The mold to fabricate the PDMS well plate was made by PTFE and glass. The PTFE and glass mold were washed by sonication in ethanol, methanol, and DI water for 10 min each. Particularly for the glass plate, trichlorosilane (Tokyo Chemical Industry) was coated to give hydrophobicity on the glass plate, enhancing the PDMS molding technique. The PDMS well plates were made into a 24-well plate size. The PDMS prepolymer and curing agent were mixed in a 10:1 ratio and degassed in a vacuum oven. The PDMS substrates were cured in 90 °C for 1 h and well covered with a 24-well plate-sized PDMS. All PDMS substances were washed in ethanol, methanol, and DI water, and UV sterilized for 10 min each before the experiment. A 2 mL of the Nb2Se9 solution dispersed by poloxamer 188 was filtered on the AAO membrane with a pore diameter of 100 nm to form the solid thin film. The Nb2Se9 film was dry transferred to the PDMS substrate to evaluate the proliferation of the C2C12 cells on it.
Live/dead assay and fluorescence imaging
The 2 × 104 cells were seeded in a molded PDMS well plate and incubated for the required time. To avoid cell damage on the substrate, half of the culturing media was replaced every day. The LIVE/DEAD viability/cytotoxicity kit for mammalian cells (Thermo Fisher) was used for fluorescence imaging, in which 20 μL of the supplied ethidium homodimer-1 (EthD-1) was added to 10 mL of PBS and mixed well, followed by the addition of 5 μL of the supplied calcein AM. The cell-culturing media in the samples were removed, and the samples were washed twice with PBS, followed by treatment with the assay solution for 30 min in the dark. An inverted microscope (Olympus, X71) was used for imaging the live and dead cells. Green fluorescence (calcein, excitation (Ex)/emission (Em) = ~ 494/517 nm) indicated live cells, while red fluorescence (EthD-1, Ex/Em = ~ 528/617 nm) indicated dead cells29.
Proliferation of C2C12 on substrate
All experiments were conducted using the same method described in the previous section. The MTT assay was conducted with the same method mentioned in the “Proliferation of C2C12 with Nb2Se9 dispersed by poloxamer 188” section. After the DMSO treatment, formazan solution was aliquoted into the new 96-well plate for the reader29.
Change history
13 April 2021
A Correction to this paper has been published: https://doi.org/10.1038/s41598-021-88217-4
References
Duan, X., Huang, Y., Cui, Y., Wang, J. & Lieber, C. M. J. N. Indium phosphide nanowires as building blocks for nanoscale electronic and optoelectronic devices. Nature 409, 66–69 (2001).
Kim, S. et al. High-mobility and low-power thin-film transistors based on multilayer MoS2 crystals. Nat. Commun. 3, 1011 (2012).
Xia, F., Mueller, T., Lin, Y.-M., Valdes-Garcia, A. & Avouris, P. J. N. Ultrafast graphene photodetector. Nat. Nanotechnol. 4, 839 (2009).
Kim, H. W. et al. Efficient hydrogen peroxide generation using reduced graphene oxide-based oxygen reduction electrocatalysts. Nat. Catal. 1, 282–290 (2018).
Kim, H. W. et al. Selective gas transport through few-layered graphene and graphene oxide membranes. Science 342, 91–95 (2013).
Patil, A. J., Vickery, J. L., Scott, T. B. & Mann, S. J. A. M. Aqueous stabilization and self-assembly of graphene sheets into layered bio-nanocomposites using DNA. Adv. Mater. 21, 3159–3164 (2009).
Elfström, N., Karlström, A. E. & Linnros, J. J. N. L. Silicon nanoribbons for electrical detection of biomolecules. Nano Lett. 8, 945–949 (2008).
Nanda, S. S. et al. A new device concept for bacterial sensing by Raman spectroscopy and voltage-gated monolayer graphene. Nanoscale 11, 8528–8537 (2019).
Huang, X., Jain, P. K., El-Sayed, I. H. & El-Sayed, M. A. J. L. I. M. S. Plasmonic photothermal therapy (PPTT) using gold nanoparticles. Laser Med. Sci. 23, 217 (2008).
Jun, Y.-W. et al. Nanoscale size effect of magnetic nanocrystals and their utilization for cancer diagnosis via magnetic resonance imaging. J. Am. Chem. Soc. 127, 5732–5733 (2005).
Li, X., Wang, X., Zhang, L., Lee, S. & Dai, H. J. S. Chemically derived, ultrasmooth graphene nanoribbon semiconductors. Science 319, 1229–1232 (2008).
Schwierz, F. J. N. N. Graphene transistors. Nat. Nanotechnol. 5, 487 (2010).
Ding, N. et al. Tellurium@ ordered macroporous carbon composite and free-standing tellurium nanowire mat as cathode materials for rechargeable lithium-tellurium batteries. Adv. Energy Mater. 5, 1401999 (2015).
Ploscaru, M. I. et al. Mo6S9-xIx nanowire recognitive molecular-scale connectivity. Nano Lett. 7, 1445–1448 (2007).
Kozlova, M. N. et al. Synthesis, crystal structure, and colloidal dispersions of vanadium tetrasulfide (VS4). Chem. Eur. J. 21, 4639–4645 (2015).
Agyapong-Fordjour, F.O.-T. et al. One-dimensional single-chain Nb2Se9 as efficient electrocatalyst for hydrogen evolution reaction. ACS Appl. Energy Mater. 2, 5785–5792 (2019).
Zhang, Q. et al. Thermal transport in quasi-1D van der Waals crystal Ta2Pd3Se8 nanowires: size and length dependence. ACS Nano 12, 2634–2642 (2018).
Juarez, B. H., Ibisate, M., Palacios, J. M. & López, C. J. A. M. High-energy photonic bandgap in Sb2S3 inverse opals by sulfidation processing. Adv. Mater. 15, 319–323 (2003).
Choi, Y. C. et al. Sb2Se3-sensitized inorganic–organic heterojunction solar cells fabricated using a single-source precursor. Angew. Chem. Int. Ed. 53, 1329–1333 (2014).
Chae, S. et al. Design of dispersant structures for preparing highly concentrated one-dimensional inorganic molecular chains from V2Se9 crystals. Chem. Commun. 54, 12190–12193 (2018).
Island, J. O. et al. Ultrahigh photoresponse of few-layer TiS3 nanoribbon transistors. Adv. Opt. Mater. 2, 641–645 (2014).
Liu, G. et al. Low-frequency electronic noise in quasi-1D TaSe3 van der Waals nanowires. Nano Lett. 17, 377–383 (2017).
Xiong, W.-W., Chen, J.-Q., Wu, X.-C. & Zhu, J.-J. Visible light detectors based on individual ZrSe3 and HfSe3 nanobelts. J. Mater. Chem. C 3, 1929–1934 (2015).
McCarten, J., DiCarlo, D., Maher, M., Adelman, T. & Thorne, R. Charge-density-wave pinning and finite-size effects in NbSe3. Phys. Rev. B 46, 4456 (1992).
Ning, W. et al. Superconductor–insulator transition in quasi-one-dimensional single-crystal Nb2PdS5 Nanowires. Nano Lett. 15, 869–875 (2015).
Chung, D.-Y. et al. CsBi4Te6: a high-performance thermoelectric material for low-temperature applications. Science 287, 1024–1027 (2000).
Kim, B. J. et al. Thickness-dependence electrical characterization of the one-dimensional van der Waals TaSe3 crystal. Materials 12, 2462 (2019).
Kim, B. et al. Exfoliation and characterization of V2Se9 atomic crystals. Nanomaterials 8, 737 (2018).
Lee, J. W. et al. Single-chain atomic crystals as extracellular matrix-mimicking material with exceptional biocompatibility and bioactivity. Nano Lett. 18, 7619–7627 (2018).
Nicolosi, V., Vengust, D., Mihailovic, D., Blau, W. J. & Coleman, J. Debundling by dilution: observation of significant populations of individual MoSI nanowires in high concentration dispersions. Chem. Phys. Lett. 425, 89–93 (2006).
Chae, S. et al. Highly concentrated single-chain atomic crystal LiMo3Se3 solution using ion-exchange chromatography. Chem. Commun. 54, 12503–12506 (2018).
Bettinger, C. J. et al. Three-dimensional microfluidic tissue-engineering scaffolds using a flexible biodegradable polymer. Adv. Mater. 18, 165–169 (2006).
Bianco, A., Kostarelos, K. & Prato, M. Applications of carbon nanotubes in drug delivery. Curr. Opin. Chem. Biol. 9, 674–679 (2005).
Karakoti, A. S., Das, S., Thevuthasan, S. & Seal, S. PEGylated inorganic nanoparticles. Angew. Chem. Int. Ed. 50, 1980–1994 (2011).
Chae, S. et al. Design of dispersant for highly concentrated one-dimensional Nb2Se9 inorganic molecular chains from bulk crystal. Sci. Rep. 9, 1–6 (2019).
Kang, J. et al. Thickness sorting of two-dimensional transition metal dichalcogenides via copolymer-assisted density gradient ultracentrifugation. Nat. Commun. 5, 5478 (2014).
Oh, S. et al. Inorganic molecular chain Nb2Se9: synthesis of bulk crystal and one-atom-thick level exfoliation. Phys. Status Solidi RRL 12, 1800451 (2018).
Acknowledgements
This research was supported by Leading University Project for International Cooperation through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE) (NRF-2019R1F1A1063170); and partially supported by NRF-2019R1A2C1006972. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT). (No. 2020R1A2C2010984).
Author information
Authors and Affiliations
Contributions
J.-Y. C. designed the experiments, and S. C. and S. O. performed the materials synthesize and dispersion experiments. J. W. L and J. H. L. support the MTT assay measurement. K. H. C. and Z. L. and J. J. and G. A. and J. K. support structural analysis of dispersed materials. C. W. and C. L. and X. D. and C. W. and L. S. and S. J. K. and S. Y. S. support the fabrication of MTT substrate using PDMS. J. H. L. and H. K. Y. and J.-Y. C. conceived and supervised this study and provided intellectual and technical guidance.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Chae, S., Oh, S., Choi, K.H. et al. A study on the bio-applicability of aqueous-dispersed van der Waals 1-D material Nb2Se9 using poloxamer. Sci Rep 11, 176 (2021). https://doi.org/10.1038/s41598-020-80730-2
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-020-80730-2
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.