Table 1 Comparison of the droplet RT-PCR assay with the CDC qRT-PCR assay for detection of SARS-CoV-2.

From: Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification

Target Digital droplet RT-PCR CDC qRT-PCR
N-gene N-gene
Limit of detection  < 10 copies/µL input  < 10 copies/µL input
Quantification Absolute quantification (number of positive droplets) Relative quantification (standard curve is required)
Inhibitor tolerance Tolerant Sensitive
Assay reaction time (approximate) 120 min 120 min
Assay sample-to-result time (approximate) 5 h 4 h
Estimated cost per reaction $8.63 $1.48