Figure 1 | Scientific Reports

Figure 1

From: Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification

Figure 1

Comparison of the CDC SARS-CoV-2 detection assay requiring RNA extraction followed by bulk qRT-PCR (top workflow) versus our ddPCR approach directly on crude lysate (bottom workflow). Red molecules represent target nucleic acids while black molecules represent viral proteins and other reaction inhibitors present in unpurified cell lysate. Digital droplet PCR potentially improves viral load quantification by sequestering reaction inhibitors in separate droplet from target nucleic acid sequences.

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