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# Genome-wide association studies in tropical maize germplasm reveal novel and known genomic regions for resistance to Northern corn leaf blight

## Abstract

Northern Corn Leaf Blight (NCLB) caused by Setosphaeria turcica, is one of the most important diseases of maize world-wide, and one of the major reasons behind yield losses in maize crop in Asia. In the present investigation, a high-resolution genome wide association study (GWAS) was conducted for NCLB resistance in three association mapping panels, predominantly consisting of tropical lines adapted to different agro-ecologies. These panels were phenotyped for disease severity across three locations with high disease prevalence in India. High density SNPs from Genotyping-by-sequencing were used in GWAS, after controlling for population structure and kinship matrices, based on single locus mixed linear model (MLM). Twenty-two SNPs were identified, that revealed a significant association with NCLB in the three mapping panels. Haplotype regression analysis revealed association of 17 significant haplotypes at FDR ≤ 0.05, with two common haplotypes across three maize panels. Several of the significantly associated SNPs/haplotypes were found to be co-located in chromosomal bins previously reported for major genes like Ht2, Ht3 and Htn1 and QTL for NCLB resistance and multiple foliar disease resistance. Phenotypic variance explained by these significant SNPs/haplotypes ranged from low to moderate, suggesting a breeding strategy of combining multiple resistance alleles towards resistance for NCLB.

## Introduction

Maize is the world’s leading cereal crop in terms of production, with 1016 million metric tons (MMT) produced on 184 million hectares (M ha) globally1, across tropical and temperate zones. About 80 per cent of the tropical maize is grown under rainfed conditions in sub-Saharan Africa, South and Southeast Asia, and Latin America, and is particularly vulnerable to an array of abiotic and biotic stresses. Among the biotic stresses, Northern Corn Leaf Blight (NCLB) also known as Turcicum Leaf Blight (TLB), is the most important disease of maize caused by hemi-biotrophic pathogen Setosphaeria turcica anamorph Exserohilum turcicum formerly known as Helminthosporium turcicum [Pass] Leonard and Suggs". The disease has a widespread occurrence throughout the world and shows its presence in Asia, Africa, Europe and America. Low temperature, high humidity, heavy dew and high rainfall are conducive for the proliferation of the pathogen to cause the disease. S. turcica is differentiated into various races, some of the common races being 0, 1, 2, 3, 12, 23, 23 N, 123 N, identified based on their virulence against Ht (Helminthosporium turcicum) genes Ht1, Ht2, Ht3, ht4, HtM, HtP, Htn1, HtNB, and rt in maize plants2. Ht genes are known to confer qualitative resistance which is race specific, inherited by single genes, and mostly dominant in gene action, and were initially identified in different genetic backgrounds. Expression of Ht genes in maize plants and or avirulence genes of S. turcica are altered by environmental conditions like temperature and light intensity, creating unstable and less durable resistance. While most of the Ht genes are mapped on consensus genetic maps (www.maizegdb.org), some of them have been fine mapped and cloned. Chung et al.3 characterized and mapped a region on chromosome bin 8.06 from a maize hybrid DK888, and suggested that QTL NLB8.06DK888 was identical, allelic or closely linked and functionally related to Ht2. Hurni et al.4 cloned Htn1 gene which confers quantitative and partial resistance to NCLB by delaying the onset of lesion formation. Using high resolution map based cloning, a receptor-like kinase gene was identified to be underlying the Htn1 gene.

Qualitative resistance usually leads to a high level of resistance when avirulent races dominate the fungal population, whereas some Ht genes can easily turn ineffective in case of the occurrence of a virulent strain2. In temperate environments, where pathogen variability is less, pyramiding multiple Ht genes is a good strategy towards NCLB resistance breeding. In tropical environments with high pathogen abundance and variability, Ht genes were found to provide only partial resistance5. Broad-based quantitative resistance to NCLB is preferred in tropical environments, which could be achieved by quantitative disease resistance loci (dQTLs) alone, or in combination with effective Ht genes. dQTLs are loci of small effects and is less likely to be overcome by evolution of new pathogens, and therefore it is practically more useful to breeders6. Inheritance studies of quantitative NCLB resistance using classical methods have revealed predominantly additive gene action controlling the trait7,8.

Quantitative trait loci (QTL) or linkage mapping is an effective approach for studying complex and polygenic forms of disease resistance9. A number of mapping studies have been undertaken for identifying QTLs for NCLB resistance in varied germplasm and environments (Table 1). Previously reported QTL distribution for NCLB resistance has been very diffuse. Nevertheless, certain chromosomal regions are reportedly shared in multiple QTL mapping studies specifically on chromosomal bin 1.03–06, 4.04–06, 5.04–07, 8.02–03, 8.05–06 and 9.02–04 (Table 1). Meta-QTL studies on resistance to multiple foliar diseases identified about 147 multiple disease resistance mQTLs for three foliar diseases, NCLB, Southern leaf blight (SLB) and Gray leaf spot (GLS) and identified bins 3.04–08, 5.04–07, and 8.05–06 that are significant for resistance to these diseases10. QTLs on chromosome 3 bin 3.04–08 has been identified in many studies for NCLB and SLB11. QTLs on chromosome bin 5.04 and 5.06–07 were detected in different mapping studies for NCLB and GLS resistance11. QTLs on chromosome bin 8.05/8.06 has been detected in most of the QTL mapping studies for NCLB, where the major genes Ht2 and Htn1 are also mapped. This region has also been found to be important for resistance to other disease like GLS, common rust and common smut12,13,14,15. Meta-QTLs identified for multiple foliar diseases on chromosome 8 bin 8.08 were also found to be associated with two Nucleotide Binding Site (NBS) family of R genes. Meta-QTL analysis have also revealed that chromosome 8 possesses a cluster of QTLs and significant real (consensus) QTLs for NCLB, GLS and SLB with confidence interval (CI) lesser than 5 cM10 .

QTL mapping, though a powerful tool, has its own limitations such as i) limited number of recombination events during population development resulting in low mapping resolution, ii) only two alleles of each mapping population studied and iii) difficult to identify the positional candidate genes or to make strong inference on linkage relationships among other QTL identified6. In most of the QTL mapping studies, the mapping populations and breeding populations are unrelated, and hence the translation of the QTLs identified to breeding targets had been very few. GWAS, in assembled mapping panels representing the wide diversity in breeding programs, is another powerful tool to dissect complex traits and complements linkage mapping by improving mapping resolution. GWAS has been used to identify allelic variants that allow improved tolerance to various biotic and abiotic stresses in maize. Resistance to a large number of economically important and complex diseases of maize like Fusarium ear rot16, GLS17,18, head smut19, NCLB6,20,21, SLB22, sugarcane mosaic virus23, Maize streak virus24, Maize lethal necrosis25, sorghum downy mildew26 and tar spot27 have been dissected using GWAS. Several reports on GWAS for NCLB resistance in maize are available, mostly in temperate germplasm and environments. In a GWAS study conducted by Van Inghelandt et al.21, a large association mapping panel of 1487 inbred lines of temperate origin was used to dissect the genetic architecture of NCLB resistance, and reported association of significant SNPs on chromosomes 2, 5, 6 and 7 whereas, some of the SNPs were also identified on chromosomes 7 and 9 after correcting for flowering time variate. In a nested association mapping population of 4630 RILs, 208 SNPs associated with NCLB resistance on all 10 chromosomes of maize were identified, along with 29 QTLs, mostly with multiple loci6. Ding et al.20 studied the CIMMYT tropical maize germplasm that were phenotyped at different locations of Mexico and Africa for NCLB resistance and identified 12 SNPs on chromosome 3, 4, 6, 7, 8 and 10 for AUDPC, 14 SNPs on chromosome 1, 2, 3, 4, 6, 7, 9 and 10 for mean disease rating and 19 SNPs on chromosome 3, 4, 5, 7 and 10 for NCLB rating. In general, QTL mapping and GWAS studies in maize have revealed one important aspect that, NCLB resistance is a polygenic trait, and resistance due to major effects contributed by Ht genes was rare in most of the germplasm and environments studied. These studies suggested that there could be environment-specific and germplasm-specific moderate to large effect genomic regions controlling resistance to NCLB, which could be exploited in incorporating quantitative resistance to this important disease in breeding programs. Association mapping studies for NCLB resistance have been conducted by various research groups using temperate and tropical maize germplasm in American, African and European environments. However genome wide association studies using high density markers from large set of maize lines from Asian region are seldom reported. Therefore, the present research was designed to conduct GWAS for NCLB resistance under Asian conditions using tropical maize germplasm represented in three maize panels. These three panels represented maize germplasm from CIMMYT and several national partners, bred in different geographies across the tropics, and hence potentially selected in the presence of varied races of S. turcica. They represent most of the genetic diversity that is available across different tropical/sub-tropical geographies where CIMMYT breeding programs operate, and hence could be ideal resources for understanding the genetics of NCLB disease in South Asian tropics. In this study, apart from single SNP-based GWAS, we also identified haplotypes for resistance to NCLB within and across association panels representing differing germplasm backgrounds.

## Results

### Phenotypic evaluation for resistance to NCLB

Subsets of three AM panels, CIMMYT Asia Association Mapping (CAAM), Drought Tolerant Maize for Africa (DTMA) and Improved Maize for African Soils (IMAS), consisting of 376, 224 and 324 lines, respectively were evaluated for NCLB resistance across different locations/years in India. Disease severity was high in the CAAM panel, with maximum score of 5.00 on a scale of 1.00–5.00 over all the three locations, with minimum disease score of 2.02, 1.50 and 1.46 at Mandya, Arabhavi and Kashmir, respectively. The average disease score across locations was 3.74. Broad-sense heritability (h2) was moderate to high (0.58–0.70) across individual locations with presence of significant genotypic variance (P value ≤ 0.001). DTMA panel at Mandya observed a mean of 2.85 with minimum disease score of 1.97 and maximum score of 4.76. Broad sense heritability estimated was 0.53, with highly significant genotypic variance (P value ≤ 0.001). Similarly, NCLB scores in IMAS panel ranged from 1.5–4.00 at Mandya in the first year and 2.00–5.00 in the second year, with mean rating of 2.55 and 3.44 during the two years, respectively. Overall analysis across the years revealed an average disease score of 3.00 with a maximum score of 4.55 and a minimum score of 1.96 where overall heritability (h2) estimate of 0.54 was observed with 0.48 in season 1 and 0.74 in season 2, respectively. IMAS panel also revealed significant genotypic variance (P value ≤ 0.001) (Table 2). The frequency distribution of mean NCLB disease ratings followed a near normal pattern in CAAM, DTMA and IMAS (Fig. 1). All the three AM panels revealed a significantly negative genotypic correlation between NCLB scores and days to anthesis (DA) (P value ≤ 0.001) (Table 3). Hence, best linear unpredicted estimates (BLUPs) were estimated using DA as a covariate to further conduct GWAS for NCLB resistance in all association panels.

### Principal component analysis and linkage disequilibrium (LD) decay

Principal Component Analysis (PCA) was performed by using the high density Genotyping by Sequencing (GBS) data, filtered for a call rate > 0.9, minor allele frequency > 0.1 and LD pruning at r2 = 0.5. The first three principal components of each panel are depicted in Fig. 2. The CAAM panel showed moderate structure, in which the Asian lowland lines partially separated from the CIMMYT lowland germplasm whereas, QPM lines grouped with the CIMMYT lowland germplasm. The DTMA panel did not exhibit substantial differential clustering of the various lines of different adaptation categories, other than the clear separation of La Posta Sequia (LPS) lines developed under CIMMYT’s LPS population improvement program, mainly for drought tolerance. IMAS panel also revealed moderate structure with clear separation of tropical and sub-tropical maize lines, with overlapping highland and sub-tropical lines. The first three PCs explained 38.56, 19.65 and 33.98 per cent variance in CAAM, DTMA and IMAS panel, respectively.

The genome wide linkage disequilibrium (LD) was plotted as LD (r2) between adjacent pairs of markers versus the distance between adjacent markers in Kb (Fig. 3). Genome wide LD plot displayed the LD-decay in CAAM panel as 2.65 Kb at r2 = 0.1 and 0.92 Kb at r2 = 0.2, with chromosome 7 showing the fastest LD-decay (1.79 Kb at r2 = 0.1 and 0.62 Kb at r2 = 0.2).Chromosome 8 displayed the slowest LD-decay (4.69 Kb at r2 = 0.1 and 1.63 Kb at r2 = 0.2). DTMA panel showed the slowest genome wide LD-decay amongst the three panels with LD decay of 5.03 Kb at r2 = 0.1 and 1.75 Kb at r2 = 0.2. Chromosome wise LD-decay revealed that the fastest decay was in chromosome 6 (3.22 Kb at r2 = 0.1 and 1.11 Kb at r2 = 0.2) while chromosome 8 showed the slowest decay (12.81 Kb at r2 = 0.1 and 4.43 Kb at r2 = 0.2) decay. IMAS panel showed the genome LD-decay of 2.84 Kb at r2 = 0.1 and 0.99 Kb at r2 = 0.2 with chromosome 6 (2.02 Kb at r2 = 0.1 and 0.70 Kb at r2 = 0.2) and chromosome 8 (5.24 Kb at r2 = 0.1 and 1.83 Kb at r2 = 0.2) showed the fastest and slowest LD-decay, respectively (Supplementary Table 1).

### GWAS for NCLB resistance

A robust subset of SNPs from high density imputed 955 K GBS genotypic data was used to conduct GWAS with 293,606, 297,437, and 309,608 SNPs after following the filtration criteria of call rate ≥ 0.7 and minor allele frequency ≥ 0.05 in CAAM, DTMA and IMAS panels, respectively. Naïve or G-test association model showed highest genomic inflation; whereas MLM model corrected for both population structure and kinship revealed the least genomic inflation as observed in the Quantile–Quantile (QQ) plots (Fig. 4). Therefore highly significant associations for NCLB resistance in the panels were ascertained based on MLM analysis. The narrow sense heritability for NCLB resistance in CAAM, DTMA and IMAS panels was 0.56, 0.52 and 0.53, respectively based on the IBS kinship matrix employing all SNPs used in GWAS. A total of five SNPs were identified to be associated with NCLB resistance in CAAM panel with P values ranging from 5.27 × 10–07 to 8.22 × 10–06. Three SNPs in DTMA panel and 14 SNPs in IMAS panel were identified in MLM analysis, with P values ranging from 3.76 × 10–06 to 1.63 × 10–05 and 4.98 × 10–08 to 8.60 × 10–06 in DTMA and IMAS panels, respectively (Table 4). SNP S7_165196774 located on chromosome 7 and three other SNPs S8_95422954, S8_95422964 and S8_95422973 with closely placed physical co-ordinates on chromosome 8 showed the lowest P values for NCLB resistance in the CAAM panel explained phenotypic variance ranging from 5.32 to 6.68%. Similarly in DTMA panel, GWAS identified three highly significant SNPs on chromosome 7 (S7_110282525, S7_110282502 and S7_131034143), explaining phenotypic variance ranging from 8.45 to 9.65%. A group of 8 SNPs located at close physical co-ordinates near 157 Mb on chromosome 8 were among the 14 SNPs which were identified to be showing most significant association with NCLB in the IMAS panel (Table 4). SNP S8_157987611 was observed to be significantly associated at a P value of 4.98 × 10–08 and explained 9.10% of phenotypic variance. GWAS conducted on different panels identified several SNPs that have co-localized physical co-ordinates within chromosomal bins where Ht genes or QTLs for NCLB resistance were previously reported. Predicted gene annotations in the B73 maize reference genome version 2 (http://ensembl.gramene.org/Zea_mays) were studied to identify the genes based on the SNPs associated with NCLB resistance. Several significant SNP associations in these three GWAS studies were located within genes with functional domains leading to biotic and abiotic stress tolerance, immune response, metabolism, plant development and maturity and responses to abiotic stresses (Table 4).

### Haplotype detection and regression analysis for the trait

A set of 842 SNPs in the bottom 0.1 percentile of the distribution in each GWAS study detected 112 haplotype blocks across the 10 chromosomes. Haplotype Regression Analysis (HTR) was carried out with 112 haplotypes on NCLB BLUP estimates of individual maize panels separately. HTR analysis in the CAAM panel identified 21 haplotype blocks with FDR value ≤ 0.05 that explained 1.98–8.46% variance (Supplementary Table 2). Thirty nine haplotypes were found to be significantly associated with NCLB disease rating in DTMA panel with explained phenotypic variance of 2.64–13.90% (Supplementary Table 3). In IMAS panel, 38 haplotype blocks were detected to be associated with NCLB resistance explaining phenotypic variance ranging from 1.71 to 11.50% (Supplementary Table 4). HTR analysis identified 17 common haplotypes having a significant effect (FDR ≤ 0.05) on the trait in at least two different AM panels spread across seven chromosomes (1, 2, 4, 5, 8, 9 and 10), each consisting of 2–10 SNPs (Table 5; Fig. 4). The proportion of variance explained by these common haplotype blocks ranged from 1.71 to 9.42%. No haplotype was identified to have a significant effect on the trait in all three AM panels. CAAM and DTMA panels shared eight common haplotypes with significant effect on the trait, whereas six and three common haplotypes were identified between DTMA and IMAS panels and CAAM and IMAS panels, respectively that are significantly associated with NCLB disease.

## Discussion

NCLB is an important foliar disease of maize in almost all temperate and tropical maize growing regions of the world. Resistance for NCLB in maize can be achieved through breeding using qualitative and quantitative resistance, either separately or together. However, resistance provided by qualitative/major genes becomes ineffective in the presence of virulent strains. Tropical environments show high pathogen abundance and high genetic diversity which leads to inflated disease severity, and hence the chances of breakdown of resistance are high. Compared to other grass crops like rice and wheat, majority of disease resistance deployed by maize breeders are quantitative in nature, and not qualitative28. It was also noted that the major genes influencing NCLB resistance have high environmental dependence with regard to light and temperature29, and act like partial/quantitative resistance in some environments. Resistance to NCLB is considered to be a mandatory trait in breeding successful maize varieties across the tropics, and hence is an important breeding target. Therefore identifying, validating and deploying high value genomic regions for the trait will help in achieving enhanced genetic gains for the trait. Targeted molecular breeding for traits demand genetic mapping and molecular characterization of the functional genomic regions associated with the trait30. Association mapping utilizes the ancestral recombination events in a natural population to make marker-phenotype relations31. It has several advantages over linkage mapping such as, (1) existing population can be used rather than developing new bi- parental population for mapping. (2) Large number of alleles can be surveyed (3) Higher mapping resolution and (4) Lesser research time30,32.

The three association mapping panels used in this study represent most of the genetic diversity that is available across different geographies where CIMMYT breeding programs operate, and hence could be ideal resources for understanding the genetics of NCLB disease in Asia. The Trial means for NCLB scores of IMAS and DTMA panels were lesser compared to CAAM panel at Mandya, which is a location with high disease severity and where all the three panels were evaluated, indicating that higher levels of resistance is available in the African and Latin American CIMMYT germplasm, as compared to CIMMYT-Asia germplasm. One of the reasons could be that the DTMA and IMAS panel included lines predominantly adapted to Sub Saharan Africa (SSA), where large number of lines were evaluated for foliar diseases like GLS, NCLB and common rust by collaborators through a regional maize disease nursery project (REGNUR)33. However the CAAM panel lines were bred or/and selected for the Asian environments, and had a history of breeding for resistance to diseases like downy mildews34,35. The CAAM panel evaluated at three locations observed highest disease score mean at Kashmir, located at higher altitude in the northern boundary of India, which may be due to highly congenial environment for disease development owing to cool and humid weather, and probable presence of more virulent races of the pathogen at that location. For the phenotyping trials, artificial inoculation was conducted at all locations with the pathogen sources collected from respective locations.

For principal component and kinship analysis, SNPs fulfilling the criteria of CR ≥ 0.9, MAF ≥ 0.1 and LD pruned at an r2 threshold of 0.5 were used. LD-pruning was done to reduce the confounding effects due to large blocks of SNPs that have strong LD with each other36. There was only moderate structure observed in the three panels, with no clear differentiation of major adaptation groups, except in the IMAS panel. The CIMMYT maize germplasm was not found to have strong population structure in various earlier studies25,26,37. George et al.38 observed that CIMMYT’s tropical and sub-tropical lines in the Asian region possess significant genetic diversity that did not allow a clear distinction into separate clusters. Warburton et al.39 observed that the CIMMYT pools and populations which served as the germplasm sources for derivation of many breeding lines in the tropical and sub-tropical adaptation groups had a large amount of diversity within, than between source populations. This heterogeneous nature of the CIMMYT populations was suggested to be responsible for the lack of a well-defined population structure in the germplasm. A rapid LD decay was observed in all the panels (0.9 kb at r2 = 0.2 for CAAM, 1.75 kb at r2 = 0.2 for DTMA and 0.99 kb at r2 = 0.2 for IMAS panel). Lu et al.40 found that the LD decay distance in temperate maize germplasm (10–100 kb) was 2 to 10 times higher than that of tropical maize germplasm (5–10 kb). Our results were more similar to the finding by Romay et al.41 that LD decays much more rapidly in the tropical germplasm to about 1 kb at r2 = 0.2. The higher LD decay in tropical germplasm suggests the more diverse genetic base that resulted from the historic recombination events and might have more rare alleles than temperate germplasm42. The LD decay was different for the 10 chromosomes in all panels, with the slowest decay observed in chromosome 8 consistently in all the panels studied. This was also observed by Suwarno et al.43 in a Carotenoid association mapping panel comprising of tropical, sub-tropical and a small proportion of temperate lines. Pace et al.44 have also observed this pattern in a sub-set of AMES panel, which is predominantly a temperate maize lines panel. This is an interesting observation and will require further probing to understand the reasons behind the slower LD decay in chromosome 8 and its implications in molecular breeding applicability in terms of traits like resistance to NCLB, with genomic regions conferred by genes/QTL located on Chromosome 8.

The single locus mixed linear model was used after correcting for population structure and familial relationships (kinship), for conducting GWAS in all the panels to reduce the genomic inflation. Highly significant SNPs associated with NCLB resistance were selected based on the significance threshold corrected for multiple testing corrections, taking average extent of genome-wide LD into consideration45. A Total of 22 SNPs significantly associated with NCLB resistance were identified on chromosomes 1, 6, 7, 8 and 10. The most significant association in the CAAM panel was with SNP S7_165196774 (P value 5.27 × 10–7), at 165.19 Mb, in the bin 7.04 (www.maizegdb.org, Maize B73 RefGen_V2), co-located within the physical interval of markers flanking the major gene Ht321. Ht3 has been introgressed from Tripsacum floridanum into maize46and Ht3 gene provides resistance against the S. turcica races 0, 1, 2, N, 12 and 2N47, by inhibiting the extension of chlorotic spots and decreasing the production of spores by the pathogen46. S. turcica races 0, 1 and 2 are prominent in the Asian countries like China and India48, and Ht3 could be effective against these races. Considering the most significant SNP identified could possibly be in LD with Ht3, owing to its physical location, it provides a strong lead to follow up for future NCLB resistance mapping and deployment efforts. A glutathione S. transferase (GST) gene, belonging to a plant-specific clade implicated in defence has also been identified in bin 7.04 that confers multiple disease resistance to NCLB, SLB and GLS49. In the DTMA panel, two highly significant SNPs (S7_110282525, S7_110282502), closely located at 110 Mb (bin 7.02) on chromosome 7, were found to be associated with NCLB resistance. Van Inghelandt et al.21 identified SNPs on bin 7.02 associated with NCLB resistance in a GWAS of 1487 maize inbred lines representing elite European and North American germplasm. Similarly, another significantly associated SNP identified in the DTMA panel (S7_131034143) was located on chromosomal bin 7.03. Dingerdissen et al.50 identified a QTL in this chromosomal bin for area under disease progression curve (AUDPC) trait in F2:3 lines derived from Mo17 and B52 at Embu, Kitale and Muguga, Kenya against the races 0 and N prevalent in Kenya.

In the IMAS panel, eight closely located SNPs, located at 157 Mb on bin 8.06 of chromosome 8, were identified to be the most significant association to NCLB resistance. In the maize genome, chromosome 8 (bin 8.05–8.06) is known to harbour genes for various defence pathways, and could be considered as one of the “complex, important and interesting” genomic region in terms of maize disease resistance, and NCLB resistance in particular3. It is considered as an important genomic region for many dQTLs and major genes like Ht2 and Htn1 for NCLB resistance28. Though there are apparent differences in the definition of qualitative and quantitative resistance, sometimes, pure qualitative and quantitative resistance are considered to be two ends of the same continuum and most resistance genes exist between the two extremes51. Chung et al.3 fine mapped a major QTL explaining a large proportion (14–62%) of phenotypic variance in NCLB resistance for the race 0 and 1 on bin 8.06, and it was described as either identical or allelic or closely linked and functionally similar to the major gene Ht2, which is partially dominant and is effective against the 0, 1, 3 and N races. Htn1 gene is also present in this genomic region, which is known to delay the lesion development up to four weeks after infection, reduce the number of lesion and delay the sporulation and found to be effective against most NCLB races5. Htn1 was cloned and found to be a wall associated receptor-like protein, and confer quantitative and partial resistance against NCLB4. Many other studies have also identified NCLB QTLs in these chromosomal bins. Poland et al.6 identified a large effect QTL at 152.2 Mb on bin 8.06, segregating in multiple NAM families. Similarly Chen et al.52 also identified a QTL in bin 8.06 for lesion width, while studying a RIL population. A major QTL was identified for AUDPC on chromosome 8 between the bins 8.05–8.06 in F2:3 populations studied for NCLB resistance53. Recently, a study conducted on a nested near isogenic line library for resistance to NCLB, also identified NILs with introgressions across centromeric region of chromosome 8 (bin 8.05), which overlaps two major genes ht2 and htn54. The fact that one of our mapping panels also identified a strongly associated set of closely located SNPs in this important chromosomal bin, indicated possible presence of a quantitatively expressed major gene or dQTL in this region present in the genetic background of the maize lines of this particular panel. S. turcica races prevalent in the locations that have been used for phenotyping in the present study are not yet reported, but physiological race 1 of S. turcica of maize was reported in the adjoining areas48. Wang et al.55 identified two minor QTLs on bin 8.03 associated with NCLB resistance in a RIL population. Our study also identified a group of closely linked three SNPs on bin 8.03 of chromosome 8 (S8_95422954, S8_95422964, S8_95422973) associated with NCLB resistance in the CAAM panel.

Haplotype regression analysis identified 17 haplotype blocks that are common across at least two panels among the three panels studied, and hence considered to be candidates for further studies towards NCLB resistance in Asian tropics. The use of haplotypes increase the phenotypic variance explained, and thus allows the identification of genomic regions responsible for controlling a large part of variation in the trait of interest56. The size of the haplotype block depends on the degree of LD present in the population57. Haplotype information can be beneficial when identifying marker phenotype associations and can offer advantages for the genetic dissection of loci underlying the complex trait20. Out of the 17 common haplotypes identified to be significant for NCLB resistance across different AM panels, eight haplotypes were shared between CAAM and DTMA panel, six were common between DTMA and IMAS panels and three haplotypes were shared between CAAM and IMAS panels. Haplotype Hap_1.1 was identified on chromosomal bin 1.06 in the CAAM and DTMA panels, and this bin is considered to be an important genomic region controlling resistance to multiple foliar diseases like NCLB, Stewart’s wilt, GLS and SLB58,59,60. Jamann et al.60 identified a receptor-like kinase gene, pan1, that underlie a QTL for NCLB in this region. Similarly, the physical co-ordinates of the SNPs forming the haplotype block Hap_9.1 identified in DTMA and IMAS panels fall within the confidence interval of qMdr9.02 reported for multiple disease resistance to NCLB, GLS and SLB61. Another haplotype identified on chromosomal bin 9.03 (Hap_9.2) identified in CAAM and DTMA panels was found to be located in close physical proximity to two closely spaced SNPs at 99.41 Mb identified in the Iodent material in a GWAS study conducted by Van Inghelandt et al.21. QTLs for resistance to NCLB and for multiple disease resistance on chromosomal region 4.05 have been identified in various studies11,59,62, and our study also identified two haplotype blocks Hap_4.2 and Hap_4.3 in this chromosomal bin. Overall, it was found that several SNPs/haplotypes identified in this study are in close proximity to previously reported major genes and QTL clusters, but many novel genomic regions were also discovered that could be environment and germplasm-specific.

Some of the SNPs identified in this study were found to be located in annotated genes (B73 RefGen_V2) with functional domains implicated in defence mechanisms in crops like maize, rice, and Arabidopsis. Highly significant SNP S7_165196774, identified in the CAAM panel is located in the gene GRMZM2G116426, having functional domains of alpha/beta-Hydrolases (ABH) superfamily proteins. ABHs support a variety of unique catalytic functions for defence and hormone regulation63. ABH esterase regulates the response of salicylic acid in plants, which is a key hormone to plant immune responses64. Highly significant SNPs identified in the DTMA panel on chromosome 7 are located within GRMZM2G334165 gene coding for protein kinase superfamily. Protein kinases play a central role in signalling during pathogen recognition and the subsequent activation of plant defence mechanisms. The microbial (pathogen) elicitors, also known as pathogen-associated molecular patterns (PAMPs), are recognized by the membrane-localized pattern recognition receptors (PRRs) of plants65. Transmembrane receptor kinases are one of the PRRs which help in plant defence mechanism. Eight significantly associated SNPs on chromosome 8 in the IMAS panel were found to be located in GRMZM2G319130 gene putatively coding for regulator of chromosome condensation (RCC1) family protein. RCC1 proteins contain plant specific disease resistance, zinc finger, chromosome condensation (DZC) domain66, and RML3 gene implicated in resistance to Leptosphaeria mculans in Arabadopsis was found to have RCC1 domain. It was also found to be effective for broad spectrum resistance against several necrotrophic fungi. Two genes BQ081031 and BQ080005 encoding candidate regulators of RCC1 family protein were found to be down-regulated specifically in the resistant reaction following Phytophthera. sojae infection which causes stem and root rot in soybean67.

## Conclusion

From three GWAS panels genotyped at high density, and phenotyped for NCLB disease under artificial disease pressure in multiple environments in India, 22 significant SNP associations were identified. Seventeen haplotypes were identified which were significantly associated with the trait across two or more panels studied. Several SNPs/haplotypes identified in this study were located within or in close proximity to major genes like Ht3, Ht2 and Htn1 and many previously reported dQTLs, and multiple foliar disease resistant QTL. These regions will be candidates for further validation studies and possible utilization in the breeding programs in Asia. Considerable differences were observed among different germplasm in terms of resistance to NCLB, and hence it is suggested to bring together diverse sources of resistance alleles to improve resistance to NCLB.

## Materials and methods

### Plant material

Three association mapping panels CAAM, DTMA and IMAS panels assembled by CIMMYT, Global Maize Program were used to study genome wide association for NCLB resistance. The CAAM panel included 419 tropical/ sub-tropical lines from the different breeding programs of CIMMYT adapted to Asian ecologies. This diverse panel included the lines derived from the different source populations for drought, waterlogging, heat stress, acid soil tolerance and downy mildew resistant lines. The panel has early, medium and late maturing lines with predominantly yellow kernel color. This panel has been earlier studied for GWAS for traits like root traits under drought68 and resistance for sorghum downy mildew26. The DTMA panel consisted of 285 elite inbred lines which include CIMMYT’s drought tolerant (DT) lines, with reasonable resistance to foliar diseases and insect pests. Apart from drought tolerant lines derived from various selection cycles of the DT populations like DTP1, DTP2 and La Posta Sequia, the panel also included the elite set of lines from CIMMYT breeding programs in Latin America, eastern and southern Africa, and a large set of lines from the multiple borer resistant populations developed at CIMMYT, Mexico. The lines belong to medium to late maturity group with mostly white kernel color. GWAS was previously conducted in the DTMA panel for resistance to various biotic stresses like NCLB, Maize streak virus, Maize lethal necrosis, and Tar spot20,24,25,27. The IMAS panel constituted of 380 inbred lines which included elite CIMMYT Maize Lines (CMLs), lines developed from CIMMYT breeding programs in Kenya, Zimbabwe and Mexico, and lines developed by national partners in Kenya (KALRO) and South Africa (ARC). IMAS panel was earlier used in GWAS analysis of resistance to MLN25.

### Phenotypic evaluation

#### Screening sites

CAAM panel of 419 inbred lines were evaluated for NCLB at three high disease prevalence locations for one season at Mandya (12°N; 76°E; 695 masl; 705 mm/year average annual rainfall) Arabhavi (16.2213° N, 74.8229° E; 574 masl; 495 mm/year average annual rainfall) and Khudwani, Kashmir (33.5335° N, 74.9209° E, 1560 m asml, 680 mm/year annual rainfall). The sub-set of 285 lines of DTMA panel were evaluated for one season at Mandya and the set of 380 lines of IMAS panel were evaluated at Mandya for two seasons. CAAM and DTMA panels were evaluated as replicated trials with two replications using alpha lattice design. The IMAS panel was evaluated using complete block augmented design in 40 blocks, with block size 12 and the two checks (resistant check-CML451 and susceptible check- CML474), that were replicated in each block. These trials were conducted during the rainy season as the conditions were more congenial for disease development. All entries were planted in 2 m row plot using a spacing of 0.75 m between rows and 0.20 m between plants in each row.

#### Artificial inoculation

S. turcica strains were isolated from previous year’s diseased maize leaves. Infected leaves were cut into 5–10 mm small pieces, washed with 0.6% sodium hypochlorite for 1 min and rinsed with sterile distilled water for 3–4 times under aseptic conditions. Excess water was blot dried on sterile tissue paper and infected leaf pieces were placed on Petri plates carrying pure culture Potato Dextrose Agar (PDA). The plates werse incubated at 28 °C for 3–5 days, the growing hyphal tips were transferred to PDA allowed to grow for 8–10 days at 28 °C, conidia were isolated using single spore isolation method. Pure culture of S. turcica were maintained on PDA for further use.

For artificial inoculation in the field experiments, mass multiplication of fungal culture was done on sterile sorghum grains. Approximately 200–250 g of sorghum grains were autoclaved in 500 ml conical flask, and on attaining the normal room temperature, the grains were inoculated with pure culture of S. turcica earlier grown on PDA. Flasks were incubated at 28 °C for 15–20 days until the grains were uniformly covered with fungal growth. The cultured grains were dried and ground into powder and stored in paper bags until use. Trials were inoculated by putting 1 g of ground sorghum powder into the whorl of 30 days old maize crop and the process repeated at 40 days to avoid any escapes. Soon after the inoculations, plain water was sprinkled by manual sprayer of 15 L capacity on all fungus inoculated plants. This increased the humidity and leaf wetness necessary for disease development, and thus better and more reliable phenotyping data.

#### Disease scoring

NCLB symptoms started developing after a week of artificial inoculation, however symptoms became distinguishable after reproductive growth of the plants. Disease rating in trials was recorded two times, first score was taken at 65–70 days of crop, and the second or final scoring was taken on 75th–80th day. NCLB rating was recorded using 1–5 scale69; Score 1 = highly resistant (HR) where no infection or slight infection with few lesions scattered on lower two leaves 2 = resistant (R), Light infection with moderate number of lesions scattered on lower four leaves, 3 = moderately resistant (MR) moderate to heavy infection abundant number of lesions scattered on lower leaves and few lesions on the middle leaves below the cob 4 = Susceptible (S) heavy infection, abundant number of lesions scattered on lower and middle leaves and lesions spread up to the flag leaf and 5 = highly susceptible (HS) very heavy infection, lesions scattered on almost all the leaves, plant prematurely dried.

#### Phenotypic data analysis

A Mixed linear model was used for analysis of phenotypic data from alpha-lattice design where genotypes, environments, interaction between genotype with environment and interaction with replication and environment were considered as random effects.

$$Y_{ijko} = \mu + g_{i} + l_{j} + r_{kj} + b_{ojk} + e_{ijko}$$

where Yijko is phenotypic performance of the ith genotype at the jth environment in the kth replication of the oth incomplete block, μ was an intercept term, gi was the genetic effect of the ith genotype, lj was the effect of the jth environment, rkj was the effect of the kth replication at the jth environment, bojk was the effect of the oth incomplete block in the kth replication at the jth environment, and eijko was the residual. For the CAAM panel and DTMA panel, best linear unbiased predictions (BLUPs) were estimated using Meta-R version 4.170 using anthesis date (AD) parameter as covariate because NCLB scores were significantly correlated to AD. In augmented design trials, BLUPs were estimated across years using linear model for repeated entries and linear model for entries in SAS. Linear model for repeated entry $${Y}_{{k}^{0}jl}=\mu +{\beta }_{j(l)}+{\gamma }_{l}+{\tau }_{{k}^{0}}{+({\tau \gamma )}_{{k}^{0}l}+\varepsilon }_{{k}^{0}jl}$$ ko = 1, 2, …, q (repeated entries), j = 1, 2, …, b (blocks), l = 1, 2, …, l (locations) where βj(l): is the effect of the jth block nested in lth location, γl: is the effect of the lth location, τk0: is the effect of the kth repeated entry, (τγ)k0l: is the effect of the interaction between the k0th entry and the lth location and the linear model for entries $${Y}_{ijl}=\mu +{\beta }_{j(l)}+{\gamma }_{l}+{\tau }_{i}{+({\tau \gamma )}_{il}+\varepsilon }_{ijl}$$. i = 1, 2, …, v (entries), j = 1, 2, …, b (blocks), l = 1, 2, …, l (locations). βj(l): is the effect of the jth block nested in lth location, γl: is the effect of the lth location, τi: is the effect of the ith entry, (τγ)il: is the effect of the interaction between the ith entry and the lth location. Broad- sense heritability (H2) of multi-location trials was estimated as H2 = σ2g/( σ2g + σ2ge/e + σ2e/er), where σ2g, σ2ge and σ2e are the genotypic, genotype-by-environment interaction and error variance components, respectively, and e and r are the number of environments and number of replicates within each environment included in the analysis, respectively. Meta-R version 4.1 was also used in generating descriptive statistics and genetic correlations between the NCLB scores and anthesis date.

#### DNA isolation and genotyping

DNA of all maize lines constituting association mapping panels was isolated from leaf samples of 3–4 weeks old seedlings using the standardised procedure followed by CIMMYT71 (CIMMYT 2005). Panels were genotyped at Institute for Genomic Diversity, Cornell University, Ithaca, NY, USA for Single nucleotide polymorphism (SNPs) using genotyping by sequencing method (GBS). The GBS libraries were constructed following the method of Elshire et al.ss72, and SNP calling was performed using TASSEL GBS pipeline73. Physical co-ordinates of all SNPs were derived from the maize reference genome version B73 AGPV2. The original partially imputed GBS SNP data had 955,690 genotypic data points (SNPs) across all the chromosomes of approximately 22,000 maize lines publicly available through Panzea database (www.panzea.org). For GWAS, filtration criteria of call rate (CR) ≥ 0.7 and minor allele frequency (MAF) ≥ 0.05 were used in all panels, yielding 293,606, 297,437 and 309,608 SNPs for CAAM, DTMA and IMAS panels, respectively. For estimating PCA and kinship matrix, high quality SNPs with filtering criteria of CR ≥ 0.9, MAF ≥ 0.1, and pruned at an r2 threshold of ≤ 0.5 were used for selecting 64,344 SNPs for CAAM, 69,254 for DTMA and 69,286 for IMAS panel.

#### Principal component, kinship and genome wide linkage disequilibrium analysis

The PCA method described by Price et al.74 was conducted in all panels using SNP & Variation Suite (SVS) Version_8.6.0 (SVS, Golden Helix, Inc., Bozeman, MT, www. goldenhelix.com). The first three principal components were used to project the possible population stratification among the samples using 3D plot. A kinship matrix was computed from identity-by-state (IBS) distance matrix75 as executed in SVS Version_8.6.0. $$IBS distance=\frac{No. of markers IBS2+(0.5 X No. of markers in IBS1)}{Number of non-missing markers}$$. Genome-wide LD was estimated for adjacent high quality SNPs with filtering criteria of CR ≥ 0.9, MAF ≥ 0.1 for CAAM (126,120 SNPs), DTMA (148,013 SNPs) and IMAS (139,061 SNPs) panels respectively, as adjacent-pairwise r2 values (the squared allele frequency correlations, among alleles at two adjacent SNP markers). For estimation of LD decay across the genome, r2 values between SNPs were plotted against the physical distances between the SNPs76. LD decay plot using non- linear model was plotted in R using ‘nlin’ function77. Average pairwise distances in which LD decayed at r2 = 0.2 and r2 = 0.1 were then estimated based on the model given by Hill & Weir78.

#### GWAS and haplotype regression

GWAS was carried out on AD adjusted BLUPs for NCLB resistance employing three methodologies: uncorrected genotypic data only (G-test or naïve model), genotypic data corrected for structure (Q) using 10 principle components (G + Q; general linear model (GLM)) and genotypic data corrected for both structure and kinship (K) (G + Q + K; Single locus mixed linear model (MLM)). G-test and GLM used association test with additive model and MLM used mixed model single locus (EMMAX)79 as executed in SVS Version 8.6.0. The mixed association mapping model used was Y = SNP*β + PC*α + K *μ + ε, where Y = response of the dependent variable (NCLB Score), SNP = SNP marker (fixed effects), PC = principal component coordinate from the PCA (fixed effects), K = kinship matrix (random effects), α is the vector of PC, β and μ are the vectors of SNP and K, respectively, and ε is the error. Manhattan plots were plotted using the − log 10 P values of all SNPs used in analysis; Q–Q plots were plotted of the observed − log 10 P values and the expected − log 10 P values to study the genomic inflation. Considering the genome-wide LD between SNPs, the effective number of independent markers was used to obtain the P value thresholds. The number of SNPs in linkage equilibrium with each other were estimated at an r2 threshold of 0.1. A Bonferroni corrected P value threshold at α = 1 was used to compute the significant P value thresholds45 for each panel.

SNPs within the bottom 0.1 percentile of the distribution in GWAS in each study panel were selected for haplotype detection and trait regression in all the three panels. Haplotype frequency estimation was done using the Expectation Maximisation (EM) algorithm with 50 EM iterations80, EM convergence tolerance of 0.0001 and a frequency threshold of 0.01. To minimise the historical recombination, haplotype blocks were detected based on the block defining algorithm81. Regression analysis was carried out with the haplotypes detected, based on step-wise regression of the NCLB BLUP estimates in all three panels separately with forward elimination at FDR-value cut off of 0.05.

## References

1. 1.

FAO. Food and Agricultural organisation of the United Nations (FAO), FAO statistical database. http://faostat.faao.org. (2013).

2. 2.

Galiano-Carneiro, A. L. & Miedaner, T. Genetics of resistance and pathogenicity in the maize/setosphaeria turcica pathosystem and implications for breeding. Front. Plant Sci. 8, (2017).

3. 3.

Chung, C. L., Jamann, T., Longfellow, J. & Nelson, R. Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06. Theor. Appl. Genet. 121, 205–227 (2010).

4. 4.

Hurni, S. et al. The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase. Proc. Natl. Acad. Sci. USA 112, 8780–8785 (2015).

5. 5.

Welz, H. G. & Geiger, H. H. Genes for resistance to northern corn leaf blight in diverse maize populations. Plant Breed. 119, 1–14 (2000).

6. 6.

Poland, J. A., Bradbury, P. J., Buckler, E. S. & Nelson, R. J. Genome-wide nested association mapping of quantitative resistance to northern leaf blight in maize. Proc. Natl. Acad. Sci. USA 108, 6893–6898 (2011).

7. 7.

Hughes, G. R. & Hooker, A. L. Gene action conditioning resistance to northern leaf blight in maize 1. Crop Sci. 11, 180–184 (1971).

8. 8.

Carson, M. L. Inheritance of latent period length in maize infected with Exserohilum turcicum. Plant Dis. 79, 581–585 (1995).

9. 9.

Young, N. D. QTL mapping and quantitative disease resistance in plants. Annu. Rev. Phytopathol. 34 (1996).

10. 10.

Ali, F., Pan, Q., Chen, G., Zahid, K. R. & Yan, J. Evidence of multiple disease resistance (MDR) and implication of meta-analysis in marker assisted selection. PLoS ONE 8, (2013).

11. 11.

Martins, L. B. et al. Validation and characterization of maize multiple disease resistance QTL. G3 Genes Genomes Genet. 9, 2905–2912 (2019).

12. 12.

Bubeck, D. M., Goodman, M. M., Beavis, W. D. & Grant, D. Quantitative trait loci controlling resistance to gray leaf spot in maize. Crop Sci. https://doi.org/10.2135/cropsci1993.0011183X003300040041x (1993).

13. 13.

Kerns, M. R., Dudley, J. W. & Rufener, G. K. QTL for resistance to common rust and smut in maize (Zea mays L.). Maydica 44, 37–45 (1999).

14. 14.

Lübberstedt, T., Klein, D. & Melchinger, A. E. Comparative quantitative trait loci mapping of partial resistance to Puccinia sorghi across four populations of European flint maize. Phytopathology 88, 1324–1329 (1998).

15. 15.

Lübberstedt, T., Klein, D. & Melchinger, A. E. Comparative QTL mapping of resistance to Ustilago maydis across four populations of European flint-maize. Theor. Appl. Genet. 97, 1321–1330 (1998).

16. 16.

Zila, C. T., Samayoa, L. F., Santiago, R., Butrón, A. & Holland, J. B. A genome-wide association study reveals genes associated with fusarium ear rot resistance in a maize core diversity panel. G3 Genes Genomes Genet. 3, 2095–2104 (2013).

17. 17.

Shi, L. et al. Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize (Zea mays L.). Crop J. 2, 132–143 (2014).

18. 18.

Kuki, M. C. et al. Genome wide association study for gray leaf spot resistance in tropical maize core. PLoS ONE 13, 1–13 (2018).

19. 19.

Wang, M. et al. Genome-wide association study (GWAS) of resistance to head smut in maize. Plant Sci. 196, 125–131 (2012).

20. 20.

Ding, J. et al. Genome-wide association mapping reveals novel sources of resistance to northern corn leaf blight in maize. BMC Plant Biol. 15 (2015).

21. 21.

Van Inghelandt, D., Melchinger, A. E., Martinant, J.-P. & Stich, B. Genome-wide association mapping of flowering time and northern corn leaf blight (Setosphaeria turcica) resistance in a vast commercial maize germplasm set. BMC Plant Biol. 12 (2012).

22. 22.

Kump, K. L. et al. Genome-wide association study of quantitative resistance to southern leaf blight in the maize nested association mapping population. Nat. Genet. 43, 163–168 (2011).

23. 23.

Tao, Y. et al. Combined linkage and association mapping reveals candidates for Scmv1, a major locus involved in resistance to sugarcane mosaic virus (SCMV) in maize. BMC Plant Biol. 13, 162 (2013).

24. 24.

Nair, S. K. et al. Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines. Theor. Appl. Genet. 128, 1839–1854 (2015).

25. 25.

Gowda, M. et al. Genome - wide association and genomic prediction of resistance to maize lethal necrosis disease in tropical maize germplasm. Theor. Appl. Genet. 128, 1957–1968 (2015).

26. 26.

Rashid, Z. et al. Genome-wide association study in Asia-adapted tropical maize reveals novel and explored genomic regions for sorghum downy mildew resistance. Sci. Rep. 8, 1–12 (2018).

27. 27.

Cao, S. et al. Genome-wide analysis of tar spot complex resistance in maize using genotyping-by-sequencing SNPs and whole-genome prediction. Plant Genome https://doi.org/10.3835/plantgenome2016.10.0099 (2017).

28. 28.

Wisser, R. J., Balint-Kurti, P. J. & Nelson, R. J. The genetic architecture of disease resistance in maize: a synthesis of published studies. Phytopathology 96, 120–129 (2006).

29. 29.

Thakur, R. P. Effects of temperature and light on virulence of exserohilum turcicum on corn. Phytopathology 79, 631 (1989).

30. 30.

Yu, J. & Buckler, E. S. Genetic association mapping and genome organization of maize. Curr. Opin. Biotechnol. 17, 155–160 (2006).

31. 31.

Rafalski, J. A. Novel genetic mapping tools in plants: SNPs and LD-based approaches. Plant Sci. 162, 329–333 (2002).

32. 32.

Flint-Garcia, S. A. et al. Maize association population: a high-resolution platform for quantitative trait locus dissection. Plant J. 44, 1054–1064 (2005).

33. 33.

Vivek, B. S. et al. Diallel analysis of grain yield and resistance to seven diseases of 12 African maize (Zea mays L.) inbred lines. Euphytica 172, 329–340 (2010).

34. 34.

Leon, D. E., & Lothrop, J. E. Lowland tropical germplasm development in Asia with emphasis on selection for downy mildew. (The Lowland Tropical Maize Subprogram. Maize Program Special Report. Mexieo, D.F.: CIMMYT., 1994).

35. 35.

Rashid, Z., Zaidi, P. H., Vinayan, M. T., Sharma, S. S. & Srirama Setty, T. A. Downy mildew resistance in maize (Zea mays L.) across Peronosclerospora species in lowland tropical Asia. Crop Prot. 43, 183–191 (2013).

36. 36.

Patterson, N., Price, A. L. & Reich, D. Population structure and eigenanalysis. PLoS Genet. 2, 2074–2093 (2006).

37. 37.

Wu, Y. et al. Molecular characterization of CIMMYT maize inbred lines with genotyping-by-sequencing SNPs. Theor. Appl. Genet. 129, 753–765 (2016).

38. 38.

George, M. L. et al. Genetic diversity of maize inbred lines in relation to downy mildew. Euphytica 135, 145–155 (2004).

39. 39.

Warburton, M. L. et al. Genetic characterization of 218 elite CIMMYT maize inbred lines using RFLP markers. Euphytica 142, 97–106 (2005).

40. 40.

Lu, Y. et al. Comparative SNP and haplotype analysis reveals a higher genetic diversity and rapider LD decay in tropical than temperate germplasm in maize. PLoS ONE 6, e24861 (2011).

41. 41.

Romay, M. C. et al. Comprehensive genotyping of the USA national maize inbred seed bank. Genome Biol. 14, R55 (2013).

42. 42.

Lu, Y. et al. Molecular characterization of global maize breeding germplasm based on genome-wide single nucleotide polymorphisms. Theor Appl. Genet. 120, 93–115 (2009).

43. 43.

Suwarno, W. B., Pixley, K. V., Palacios-Rojas, N., Kaeppler, S. M. & Babu, R. Genome-wide association analysis reveals new targets for carotenoid biofortification in maize. Theor. Appl. Genet. 128, 851–864 (2015).

44. 44.

Pace, J., Gardner, C., Romay, C., Ganapathysubramanian, B. & Lübberstedt, T. Genome-wide association analysis of seedling root development in maize (Zea mays L.). BMC Genom. 16 (2015).

45. 45.

Cui, Z. et al. Genome-wide association study (GWAS) reveals the genetic architecture of four husk traits in maize. BMC Genom. 17, 946 (2016).

46. 46.

Hooker, A. L. Resistance to Helminthosporium turcicum from Tripsacum floridanum incorporated into corn. Maize Genet. Coop. News Lett. 55, 87 (1981).

47. 47.

Welz, H. G. Genetics and epidemiology of the pathosystem Zea mays/Setosphaeria turcica. Habilitation Thesis. (University of Hohenheim, Stuttgart, 1998).

48. 48.

Gowda K. T.P , Sreerama Setty T. A, M. N., & P. N. Problem of turcicum leaf blight in tropical maize. In Stresses of Maize in Tropics (ed. P.H Zaidi, N. . S.) 289–297 (2005).

49. 49.

Wisser, R. J. et al. Multivariate analysis of maize disease resistances suggests a pleiotropic genetic basis and implicates a GST gene. Proc. Natl. Acad. Sci. USA 108, 7339–7344 (2011).

50. 50.

Dingerdissen’, A. L., Geiger’, H. H., Lee2, M., Schechert’, A. & Welz’, H. G. Interval mapping of genes for quantitative resistance of maize to Setosphaeria turcica, cause of northern leaf blight, in a tropical environment. Mol. Breed. 2 (1996).

51. 51.

Balint-Kurti, P. J. & Johal, G. S. Maize Disease Resistance. 229–250 (2009). https://doi.org/10.1007/978-0-387-79418-1

52. 52.

Chen, G., Wang, X., Long, S. & Jaqueth, J. Mapping of QTL conferring resistance to northern corn leaf blight using high-density SNPs in maize. Mol. Breed. 36, 1–9 (2016).

53. 53.

Welz, H. G. & Schechert, A. W. Dynamic gene action at QTLs for resistance to Setosphaeria turcica in maize. Theor Appl Genet 98, 1036–1045 (1999).

54. 54.

Kolkman, J. M. et al. Maize introgression library provides evidence for the involvement of liguleless1 in resistance to northern leaf blight. G3 Genes Genomes Genet. 10, 3611–3612 (2020).

55. 55.

Wang, J., Xu, Z., Yang, J., Lu, X. & Zhou, Z. qNCLB7. 02 , a novel QTL for resistance to northern corn leaf blight in maize (2018).

56. 56.

Maldonado, C., Mora, F., Scapim, C. A. & Coan, M. Genome-wide haplotype-based association analysis of key traits of plant lodging and architecture of maize identifies major determinants for leaf angle: HAPla4. PLoS ONE 14 (2019).

57. 57.

Slatkin, M. Linkage disequilibrium: understanding the evolutionary past and mapping the medical future. Nat. Rev. Genet. 9, 477–485 (2008).

58. 58.

Zwonitzer, J. C. et al. Mapping resistance quantitative trait loci for three foliar diseases in a maize recombinant inbred line population: evidence for multiple disease resistance?. Phytopathology 100, 72–79 (2010).

59. 59.

Lopez-Zuniga, L. O. et al. Using maize chromosome segment substitution line populations for the identification of loci associated with multiple disease resistance. G3 Genes Genomes Genet. 9, 189–201 (2019).

60. 60.

Jamann, T. M., Poland, J. A., Kolkman, J. M., Smith, L. G. & Nelson, R. J. Unraveling genomic complexity at a quantitative disease resistance locus in maize. Genetics 198, 333–344 (2014).

61. 61.

Yang, Q., Balint-Kurti, P. & Xu, M. Quantitative disease resistance: dissection and adoption in maize. Mol. Plant 10, 402–413 (2017).

62. 62.

Singh, S. R. New quantative traitloci (QTL) for turcicum leaf blight in maize. J. Biotechnol. Crop Sci. 6, 44–52 (2017).

63. 63.

Mindrebo, J. T., Nartey, C. M., Seto, Y., Burkart, M. D. & Noel, J. P. Unveiling the functional diversity of the alpha/beta hydrolase superfamily in the plant kingdom. Curr. Opin. Struct. Biol. 41, 233–246 (2016).

64. 64.

Kumar, D. Salicylic acid signaling in disease resistance. Plant Sci. 228, 127–134 (2014).

65. 65.

Zhang, J. & Zhou, J. M. Plant immunity triggered by microbial molecular signatures. Mol. Plant 3, 783–793 (2010).

66. 66.

Staal, J. & Dixelius, C. RLM3, a potential adaptor between specific TIR-NB-LRR receptors and DZC proteins. 59–61 (2008).

67. 67.

Narayanan, N. N. et al. Identification of candidate signaling genes including regulators of chromosome condensation 1 protein family differentially expressed in the soybean-Phytophthora sojae interaction. Theor. Appl. Genet. 118, 399–412 (2009).

68. 68.

Zaidi, P. H. et al. Genomic regions associated with root traits under drought stress in tropical maize (Zea mays L.). PLoS ONE 11, 1–18 (2016).

69. 69.

Payak, M. M. Maize diseases and approaches to their management in India. Trop. Pest Manag. 31, 302–310 (1982).

70. 70.

Alvarado, G. et al. ‘META-R (Multi Environment Trail Analysis with R for Windows) Version 4.1’, 6–8 (2015) http://hdl.handle.net/11529/10201. 6–8 (2015).

71. 71.

The CIMMYT Maize Program. Maize Diseases: A Guide for Field Identification. (2004).

72. 72.

Elshire, R. J. et al. A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6, 1–10 (2011).

73. 73.

Glaubitz, J. C. et al. TASSEL-GBS: a high capacity genotyping by sequencing analysis pipeline. PLoS One 9 (2014).

74. 74.

Price, A. L. et al. Principal components analysis corrects for stratification in genome-wide association studies. Nat. Genet 38, 904–909 (2006).

75. 75.

Bishop, D. T. & Williamson, J. A. The power of identity-by-state methods for linkage analysis. Am. J. Hum. Genet. 46, 254–265 (1990).

76. 76.

Remington, D. L. et al. Structure of linkage disequilibrium and phenotypic associations in the maize genome. Proc. Natl. Acad. Sci. USA 98, 11479–11484 (2001).

77. 77.

Team, R. C. R: A Language and Environment for Statistical Computing (R Foundation for Statistical Computing, Vienna, 2014).

78. 78.

Hill, W. G. & Weir, B. Variances and covariances of squared linkage disequilibria in finite populations. Theor. Popul. Biol. 31, 54–78 (1988).

79. 79.

Kang, H. M. H. et al. Variance component model to account for sample structure in genome-wide association studies. Nat. Genet. 42, 348–354 (2010).

80. 80.

Excoffier, L. S. M. Maximum-likelihood estimation of molecular haplotype frequencies in a diploid population. Mol Biol Evol 12, 921–927 (1995).

81. 81.

Gabriel, S. B. et al. The structure of haplotype blocks in the human genome. Science (80–) 296, 2225–2229 (2002).

82. 82.

Balint-Kurti, P. J., Yang, J., Van Esbroeck, G., Jung, J. & Smith, M. E. Use of a maize advanced intercross line for mapping of QTL for Northern leaf blight resistance and multiple disease resistance. Crop Sci. 50, 458–466 (2010).

83. 83.

Chung, C.-L. et al. Resistance loci affecting distinct stages of fungal pathogenesis: use of introgression lines for QTL mapping and characterization in the maize-Setosphaeria turcica pathosystem. BMC Plant Biol. 10 (2010).

84. 84.

Schechert, A. W., Welz, H. G. & Geiger, G. QTL for Resistance to Setosphaeria turcica in Tropical African Maize. Crop Sci. 39, 514–523 (1999).

85. 85.

Welz, H. G., Xia, X. C., Bassetti, P., Melchinger, A. E. & Lubberstedt, T. QTLs for resistance to Setosphaeria turcica in an early maturing Dent×Flint maize population. TAG. Theor. Appl. Genet. 99, 649–655 (1999).

86. 86.

Freymark, P. J., Lee, M., Martinson, C. A. & Woodman, W. L. Molecular-marker-facilitated investigation of host-plant response to Exserohilum turcicum in maize (Zea mays L.): components of resistance. Theor. Appl. Genet. 88, 305–313 (1994).

87. 87.

Freymark, P. J., Lee, M., Woodman, W. L. & Martinson, C. A. Quantitative and qualitative trait loci affecting host-plant response to Exserohilum turcicum in maize (Zea mays L.). Theor Appl Genet 87, (1993).

88. 88.

Fischl, R. M., Stadlmann, J., Grass, J., Altmann, F. & Léonard, R. The two endo-β-N-acetylglucosaminidase genes from Arabidopsis thaliana encode cytoplasmic enzymes controlling free N-glycan levels. Plant Mol. Biol. 77, 275–284 (2011).

89. 89.

Kimura, Y., Takeoka, Y., Inoue, M., Maeda, M. & Fujiyama, K. Double-knockout of putative endo-β-N-acetylglucosaminidase (ENGase) genes in Arabidopsis thaliana: loss of ENGase activity induced accumulation of high-mannose type free N-glycans bearing N,N’-acetylchitobiosyl unit. Biosci. Biotechnol. Biochem. 75, 1019–1021 (2011).

90. 90.

Chiu, C. C., Chen, L. J., Su, P. H. & Li, H. min. Evolution of chloroplast J proteins. PLoS ONE 8 (2013).

91. 91.

Lehti-Shiu, M. D. & Shiu, S. H. Diversity, classification and function of the plant protein kinase superfamily. Philos. Trans. R. Soc. B Biol. Sci. 367, 2619–2639 (2012).

92. 92.

Figueiredo, A., Monteiro, F. & Sebastiana, M. Subtilisin-like proteases in plant–pathogen recognition and immune priming: a perspective. Front. Plant Sci. 5 (2014).

93. 93.

Ma, Y. et al. Subfunctionalization of cation/proton antiporter 1 genes in grapevine in response to salt stress in different organs. Hortic. Res. 2 (2015).

94. 94.

Xu, N. et al. The arabidopsis NRG2 protein mediates nitrate signaling and interacts with and regulates key nitrate regulators. Plant Cell 28, 485–504 (2015).

95. 95.

Roudier, F. et al. The Medicago species A2-type cyclin is auxin regulated and involved in meristem formation but dispensable for endoreduplication-associated developmental programs. Plant Physiol. 131, 1091–1103 (2003).

96. 96.

Frank, M. J., Cartwright, H. N. & Smith, L. G. Three Brick genes have distinct functions in a common pathway promoting polarized cell division and cell morphogenesis in the maize leaf epidermis. Development 130, 753–762 (2003).

## Acknowledgements

The authors gratefully acknowledge the financial support received from the CGIAR Research Program (CRP) on MAIZE and Limagrain for funding this research work.

## Author information

Authors

### Contributions

S.N., P.H.Z. designed the experiment; Z.R., M.S., S.I.H., R.M.K., Z.A.D., P.K.S. generated phenotyping data; Z.R., S.N. analysed data; S.N., Z.R., B.S.V. wrote the manuscript. All authors reviewed the manuscript.

### Corresponding author

Correspondence to Sudha Krishnan Nair.

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### Competing interests

The authors declare no competing interests.

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Rashid, Z., Sofi, M., Harlapur, S.I. et al. Genome-wide association studies in tropical maize germplasm reveal novel and known genomic regions for resistance to Northern corn leaf blight. Sci Rep 10, 21949 (2020). https://doi.org/10.1038/s41598-020-78928-5

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