Microwave-assisted conversion of palm kernel shell biomass waste to photoluminescent carbon dots

In the present work, palm kernel shell (PKS) biomass waste has been used as a low-cost and easily available precursor to prepare carbon dots (CDs) via microwave irradiation method. The impacts of the reacting medium: water and diethylene glycol (DEG), and irradiation period, as well as the presence of chitosan on the CDs properties, have been investigated. The synthesized CDs were characterized by several physical and optical analyses. The performance of the CDs in terms of bacteria cell imaging and copper (II) ions sensing and removal were also explored. All the CDs possessed a size of 6–7 nm in diameter and the presence of hydroxyl and alkene functional groups indicated the successful transformation of PKS into CDs with carbon core consisting of C = C elementary unit. The highest quantum yield (44.0%) obtained was from the CDs synthesised with DEG as the reacting medium at irradiation period of 1 min. It was postulated that the high boiling point of DEG resulted in a complete carbonisation of PKS into CDs. Subsequently, the absorbance intensity and photoluminescence intensity were also much higher compared to other precursor formulation. All the CDs fluoresced in the bacteria culture, and fluorescence quenching occurred in the presence of heavy metal ions. These showed the potential of CDs synthesised from PKS could be used for cellular imaging and detection as well as removal of heavy metal ions.


Scientific Reports
| (2020) 10:21199 | https://doi.org/10.1038/s41598-020-78322-1 www.nature.com/scientificreports/ to scale up without suffering thermal gradient effects 20,22 . Several articles on the successful synthesis of CDs using microwave-assisted synthesis have been reported 20,[22][23][24] . For instance, using citric acid, urea, and thiourea as the raw materials, CDs with the capability in detecting mercury and iodide compounds in aqueous solution have been successfully fabricated through microwave-assisted method 23 . In another case, eggshell membrane ashes which were converted to CDs reportedly possessing photocatalytic degradation capability in reducing the methylene blue contaminants 24 .
One of the important factors that contribute to the rise of CDs is due to its easily available and low-cost carbon precursors, ranging from organic molecules, agricultural and natural products, to even the biomass wastes 1,23,[25][26][27] . Recycle and reuse of the waste with carbon-rich as precursors to prepare the CDs appears to be a good effort along with the global concern in developing a sustainable community through waste minimisation. Furthermore, the wastes are renewable resources and cheap in terms of cost 28 . Several biomass wastes have been explored by the researchers as the carbon source for the synthesis of CDs, for instance sago waste, sugarcane molasses, and food waste 7,27,28 . Huang et al. (2017) successfully converted sugarcane molasses (biomass waste) into CDs that emitted blue photoluminescence with a quantum yield of approximately 5.8% 7 . The synthesised CDs exhibited great photostability and strong resistance to photo-bleaching with no obvious change in fluorescence intensity upon continuous exposure to UV light. Furthermore, the CDs demonstrated excellent biocompatibility with living cell (bioimaging) and capable of detecting Fe 3+ and sunset yellow (synthetic organic food dye) through fluorescence quenching. These showed that CDs synthesised from natural compounds or biomass are as competent as CDs fabricated from other sources. However, the simple synthesis (using water as the reaction medium) of CDs from biomass wastes tend to have low quantum yield due to incomplete carbonisation and the precursors lack functional groups (sulfur or nitrogen atom) for better luminescence property 20,29-34 . There are several ways to improve the quantum yield of CDs from biomass wastes, such as surface passivation, heteroatom doping, and reaction conditions 30 . Of these, controlling the reaction conditions and manipulating the carbon precursors composition is of particular interest. For instance, Wee et al. (2013) observed that the rise in temperature had improved the quantum yield of the CDs 35 . This could be attributed to better carbonisation conversion rate from carbon precursors (bovine serum albumin) to CDs at higher temperature (room temperature vs 50 °C). The motion of the precursors was excited at higher temperature, and subsequently the efficiency of the carbonisation process was increased too. However, carbonisation efficiency was hindered when water was used as the reaction medium since the temperature could not rise high due to low boiling point of water. Hence, by replacing water with a high boiling point solvent such as diethylene glycol (DEG), the yield of CDs could be improved 20 . In another case, substances with amino groups (-NH 2 ) such as chitosan have been included as one of the precursors to boost the synthetic yield of CDs. The amino-functional groups which containing nitrogen improved the quantum yield due to its great fluorescence property 9,36 . Though a great number of studies have successfully improved the quantum yield and fluorescence property of CDs through heteroatom doping, majority of the studies were conducted on simple carbon precursors such as citric acid, polyacrylic acid, and glycine 23,37,38 . Similar study on natural carbon precursor has remained rare. On top of that, the reaction conditions, especially the reacting medium on the conversion of natural carbon precursor to CDs and effect on the quantum yield has never been properly investigated.
Hence, in this study, the influence of the reaction medium (DEG) and amino group compound (chitosan) on the microwave-irradiation synthesis of CDs by using natural carbon precursor-palm kernel shell (PKS), was investigated. PKS is a solid biomass waste from the palm oil industry. The palm oil industry is the most important agricultural sector in Malaysia and associated with its role in contributing to the economic growth, a significant amount of PKS (> 2.4 million tons yielded per annum) has been generated 39 . This solid waste has low economic value and is normally disposed as landfill or used as a low-rank fuel to be burned for heat/steam generation. Converting this low-cost solid waste to useful CDs will help to resolve the issues of waste management and at the same time supporting sustainable development. The potential of the synthesised CDs was further explored for cellular imaging, detection and removal of heavy metal ions. Synthesis of CDs. The PKS was ground into a powder and sieved with a sieve size of 0.2 mm to obtain fine powder for CDs synthesis. Firstly, an appropriate mass of PKS powder was weighed and then mixed with the three solutions: UPW (Set A); UPW with chitosan (amount of chitosan was the same as PKS) (Set B); and DEG (Set C), separately, to form a mixture with PKS concentration of 10 g/L in a Duran bottle. DEG was included as the reaction medium in this study to investigate its influence on the CDs as it has higher boiling point that might facilitate a more complete carbonization process 20 . The mixtures were homogenised by sonicating the solutions for 30 min. The samples were then heated in the microwave oven (Midea MM720CXM, Malaysia) at medium heating power (385 W) and heating period ranging from 1 to 5 min. Table 1 shows the labelling for each set with varied heating periods and solution formulation. The resulting product was subjected to cooling process to room temperature after the carbonisation process. The mixture was then filtered with 0.45 μm membrane filter, and the filtrate was centrifuged at 10,000 rpm for 20 min to remove the bulk particles. Finally, the supernatant was filtered again with syringe filter (0.22 μm) and then collected for further analysis and application testing. States) was used to obtain the high-resolution images of the synthesised CDs. The functional groups that present on the PKS and CDs were characterised by using Fourier Transformed Infrared (FTIR) (Nicolet, 6700, United States). UV-visible absorption spectra of the CDs were determined by using Lambda 950 UV-visible spectrophotometer (Perkin Elmer, United States). Photoluminescence spectra of the CDs was determined via FLS920 fluorescence spectrophotometer (Edinburgh Instrument, United Kingdom). The Raman spectra were obtained using Raman spectrophotometer equipped with a laser source emitting a wavelength of 532.230 nm (WITec, Model: Alpha 300R, USA). X-ray diffraction (XRD) spectra were generated via X-ray diffractometer (Bruker D8 Advance, Germany). The sample preparation for XRD analysis involved the dropping and drying of CDs on a glass slide for several cycles. The surface chemical composition of the CDs was analyzed by X-ray photoelectron spectroscopy (XPS) (Ultra DLD XPS Kratos, Manchester, UK) equipped with a monochromatic Al Kα radiation source (1486.6 eV).

Methodology
Measurement of quantum yield. The quantum yield of the prepared CDs was calculated according to the established method by using the following equation: where η, A, Φ, and I are the refractive index of the solvent, absorbance at the excitation wavelength, quantum yield, and integrated fluorescence intensity, respectively 22 . The subscript R refers to the reference fluorophore of known quantum yield while the terms with the subscript s indicate the synthesized CDs. In this study, quinine sulfate was used as the reference fluorophore. To prepare the solution for measurement, quinine sulfate (Φ R = 0.54) was dissolved in 0.1 M H 2 SO 4 (η = 1.33) and CDs were dissolved in deionized water (η = 1.33). Absorbance in the 1 cm quartz cuvette was kept below 0.10 at an excitation wavelength of 370 nm to minimize reabsorption effects 40 .
Cellular imaging. The culture of Escherichia coli (E. coli) and Bacillus subtilis bacterial cells were carried out in a sterile environment by using the equipment that had been sterilized in autoclave. The bacterial cells were cultured in sterile nutrient agar. The colony of the bacterial cells was taken from the agar plates and then incubated in 10 mL of nutrient broth. The bacterial cultures were grown overnight at 37 °C in a shaking incubator. On the next day, 150 µL of CDs solution was diluted to 600 µL with ultrapure water for the reaction with 400 µL of bacterial cells for 60 min at 37 °C with gentle shaking. To remove the supernatant, the mixture solutions were then centrifuged at 10,000 rpm for 10 min. The residue was then washed with phosphate-buffered saline (PBS) buffer and suspended in PBS buffer 41 . Lastly, the cells were observed under inverted fluorescence microscope (Nikon TI-E, Japan) after 20 µL of culture has been added to the glass slide 40 .

Results and discussion
Determination of optimum synthesis conditions. This study was started with the determination of optimum microwave irradiation synthesis conditions. As one of the key factor, the heating period played an important role in determining photoluminescence intensity of CDs 20 . The heating period has been studied to obtain optimum synthesis condition in preparing CDs with the highest quantum yield from different reaction www.nature.com/scientificreports/ medium. As shown in Table 2, the highest quantum yield for CDs synthesized from set A (26.3%), set B (26.3%), and set C (44.0%) were obtained at the heating duration of 4 min (A4), 5 min (B5), and 1 min (C1), respectively. The relatively shorter optimal heating duration and higher quantum yield for set C indicate the contribution from the DEG reacting medium where further explanation would be provided in the following section. Unexpectedly, the quantum yield of set B CDs did not differ from set A, indicating that the chitosan did not react with the PKS to form CDs with amino functional groups as observed in other studies 33 . The CDs samples (A4, B5, and C1) were then characterized and analyzed to understand the factors contributing to this variation in quantum yield.
Characterization of CDs. The size and morphology of synthesised CDs under optimal synthesis conditions were observed by TEM. Figure 1A-C shows that the synthesized CDs of sample A4, B5 and C1 were quasispherical nanoparticles with and average diameter of 6.60 nm, 6.80 nm and 7.00 nm, respectively. The CDs were   www.nature.com/scientificreports/ of PKS into CDs with carbon core consisting of C=C elementary unit 20 . The C1 sample was also accompanied by the appearance of new bands (C-O-C stretching) at 1129.3 cm −1 and 1060.0 cm −1 as well as C-H stretching with spectrum between 2850-3000 cm −1 . The presence of asymmetric stretching vibrations of C-O-C could be a sign of more complete intermolecular dehydration and polymerisation that only took place at higher temperature, as reported in other literature 32 . Since DEG has higher boiling point than water, it allowed a more complete reaction for the formation of CDs. Surprisingly, amino groups (-NH 2 ) were not detected for B5 CDs, which might be a sign that chitosan did not react well with the PKS to form CDs with amino functional groups. Past literature suggested that simple molecules such as acetylacetone could react with nitrogen functional group compounds (e.g. ammonia) to form amide-rich organic precursors 32 . These amide-rich organic precursors were then hydrolyzed and carbonized to form carbon core with surface modified with a large number of nitrogen-containing functional groups. Huge and complex natural carbon precursors such as PKS in this case might react poorly with chitosan or lack of reactive functional groups for interacting with chitosan. Subsequently, the CDs formed did not show the presence of amino functional groups. Based on the IR spectrum of all sets of CDs, the functional group that contributed to characterization of CDs is oxygen-containing functional groups such as O-H, C=O and C-O-C stretching. These oxygen-containing functional groups found on the surface of the synthesised CDs most likely were derived from the oxidation of hydroxyl groups in PKS during carbonisation process, which played an important role in determining the photoluminescence intensity of CDs 20

Photoluminescence properties. The CDs solutions appeared transparent or translucent under visible
light except the CDs prepared with DEG medium, which acquired light brown colour (Fig. 4). The colour of all the CDs samples remained stable for several weeks. As shown in Fig. 4, CDs from set A and set B emitted a weak blue luminescence while set C emitted a strong blue luminescence upon excitation under a 365 nm UV light. The brightness of the luminescence observed is closely linked to the photoluminescence properties of the CDs, which would be further elaborated below.
The UV-Vis absorption spectra of all sets of CDs were presented in Fig. 5. Based on all the UV-Vis absorption spectra obtained, strong absorption peak at 280 nm along with a shoulder around 300 nm were observed. The peak at 280 nm was due to blue-shifted π − π * transition of the conjugated C=C groups from the carbon core 31 . Meanwhile, the shoulder was attributed to the n − π * transition of C=O groups (typical characteristic of fluorescent CDs) found on the surface of CDs 31,32 . According to the trends of UV-Vis absorption spectra, it can be said that the absorption intensity for set A and B increased when heating period was prolonged. This could be an indication that more carbon cores were generated with the increase of reaction time. Such postulation was supported with the finding reported by He et al. (2017) and aligned well with the optimal quantum yield for set A and B CDs (more CDs indicate higher quantum yield) at longer heating duration 31,32 . For CDs synthesised with DEG as the reacting medium (Fig. 5c), the absorbance intensity also increased with the heating duration. However, further heating the CDs for set C has probably damaged the surface (Set C3-C5 possessed quantum yield less than 6%) and resulted in the loss of capability responding to light. Figure 6 shows the photoluminescence spectra of CDs for set A, B, and C, respectively, at the excitation wavelength of 370 nm. Based on the photoluminescence spectra obtained, CDs achieved the highest photoluminescence intensity at heating periods of 4 min, 5 min, and 1 min for set A, B, and C, respectively. According to the previous studies, photoluminescence intensity increased when the microwave irradiation periods increased (generation of more CDs) 24 . But the photoluminescence intensity will decrease at certain heating periods when turn-off phenomenon occurs. Over the optimal heating period, the CDs formed would experience surface damage that reduces the photoluminescence intensity. However, for the CDs synthesised with DEG, the highest photoluminescence intensity was achieved at the shortest heating duration. This could be due to the higher boiling point of DEG that facilitated fast heating for the formation of CDs, and further heating would lead to the damage of CDs surface (as verified by the decrease of quantum yield from 44.0% at 1 min to 3.9% at 5 min of microwave irradiation duration). Table 3 presents the emission peak of all sets of synthesised CDs upon excitation at 370 nm. The emission peaks of Set C CDs exhibited moderate redshift from 438 to 459 nm with the increase of heating period 32 . This phenomenon was probably due to the variation of CDs sizes and the surface states 29 .   Figure 8 show the effect of copper (II) ions concentration on the emission spectra of solution dosed with A4, B5, and C1 CDs, respectively. It can be seen that the photoluminescence intensity of the solutions decreased with the increase of copper (II) ions concentration from 0.125 to 0.5 M, which is an indication confirming the potential of the synthesised CDs for metal ions sensing. The fluorescence of CDs could be related to the oxygeneous functional groups such as hydroxyl and carboxylate groups (as discussed previously) present on the CDs surfaces. These functional groups are excellent chelating ligands for metal ion complexation 45,47 . The complexation between CDs and metal ions would lead to fluorescence quenching due to the formation of non-fluorescence complex between the surface functional groups of CDs and copper (II) ions 42,47 . The fluorescence quenching could be attributed to the electron/hole recombination annihilation through an efficient and reversible electron/hole transfer process 23,46 . As illustrated in Fig. 9, the photoluminescent emission of CDs which originates from the radiative recombination of excitons could be quenched by copper (II) via nonradiative electron-transfer from CDs to copper (II) ions 34 . This electron transfer pathway did not emit fluorescence and support the quenching phenomena when copper (II) ions were added to CDs solution. The degree of disturbances on the fluorescence of CDs increased with the presence of more metal ions, where it could be clearly seen that the photoluminescence intensity of all sets of CDs flattened when the concentration of copper (II) ions reached 0.5 M. The quenching efficiency for A4, B5, and C1 varied between 89-95%, 75-91%, and 85-97%, respectively. The slightly higher quenching efficiency for C1 could be attributed to its higher quantum yield that provided more oxygen-containing functional groups to interact with the metal ions. www.nature.com/scientificreports/ www.nature.com/scientificreports/ Removal of heavy metal ions. Figures 10 and 11 show the removal efficiencies of copper (II) ions under various contact times and CDs dosages. Obviously, C1 CDs outperformed A4 and B5 in terms of removal rate and efficiency. C1 CDs took 10 min to obtain 47% removal efficiency while A4 and B5 CDs failed to attain this removal efficiency even after 50 min. In another case, 2.5 ml of C1 CDs could remove 73% of copper (II) ions, showing higher removal capacity as compared to A4 and B5 (27% removal) at the similar dosage. Both these observations could be attributed to the higher quantum yield of C1 CDs with more functional groups that resulted in more CDs with functional groups to interact with the copper (II) ions. The removal process of heavy metal ions probably took place through the adsorption mechanism by electrostatic interaction between the CDs and copper (II) ions. The negatively charged hydroxyl groups on CDs could interact with the positively charged copper (II) through electrostatic interaction and subsequently led to the removal of the metal ions 51 .   www.nature.com/scientificreports/

Conclusions
In summary, it can be concluded that PKS, a biomass waste from the palm oil industry, can be the low-cost and easily available precursor for the synthesis of CDs via microwave irradiation method. It was discovered that DEG being a reacting medium with high boiling point can facilitate the fast and more complete formation of CDs (shorter duration) from PKS as compared to water as the reacting medium. This was translated to the production of CDs with higher quantum yield (44.0% vs 26.3%) which displayed better absorbance and photoluminescent intensities. Surprisingly, the presence of chitosan did not improve the quantum yield of CDs of set B, which could be a sign that chitosan did not interact with complex carbon precursors such as PKS to form CDs with amino functional groups. Nonetheless, all the synthesised CDs have been successfully used in biological cell imaging, a sign that shows the CDs could be nontoxic to living bacteria. On top of that, the CDs could be used to detect copper (II) ions through fluorescence quenching. The higher quantum yield of CDs synthesised from DEG reacting medium has provided it a greater adsorption capability as shown by its better and faster removal of copper (II) ions. Overall, the CDs synthesised from PKS possessed the potential to be used for bioimaging and detection of pollutants.