Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Daily supplementation with the Lab4P probiotic consortium induces significant weight loss in overweight adults

Abstract

This 9-month randomised, parallel, double-blind, single-centre, placebo-controlled study (PROBE, ISRCTN18030882) assessed the impact of probiotic supplementation on bodyweight. Seventy overweight Bulgarian participants aged 45–65 years with BMI 25–29.9 kg/m2 received a daily dose of the Lab4P probiotic comprising lactobacilli and bifidobacteria (50 billion cfu/day). Participants maintained their normal diet and lifestyle over the duration of the study. The primary outcome was change from baseline in body weight and secondary outcomes included changes in waist circumference, hip circumference and blood pressure. A significant between group decrease in body weight (3.16 kg, 95% CI 3.94, 2.38, p < 0.0001) was detected favouring the probiotic group. Supplementation also resulted in significant between group decreases in waist circumference (2.58 cm, 95% CI 3.23, 1.94, p < 0.0001) and hip circumference (2.66 cm, 95% CI 3.28, 2.05, p < 0.0001) but no changes in blood pressure were observed. These findings support the outcomes of a previous shorter-term Lab4P intervention study in overweight and obese participants (PROMAGEN, ISRCTN12562026). We conclude that Lab4P has consistent weight modulation capability in free-living overweight adults.

Introduction

The gastro-intestinal (GI) microbiota is a contributor to human health and has been implicated in many metabolic processes including the digestion and absorption of nutrients and the fermentation of undigested carbohydrates into short chain fatty acids and other metabolites1. The link between dysbiosis and obesity is well recognised but nearly 40% of the adults worldwide are categorised as overweight2 and this population gains on average 0.2–1.0 kg/year3. In addition to progressive weight gain, the aging process itself is characterised by changes in the gut microbiota and the development of a chronic inflammatory status4 which is linked to weight associated comorbidities such as diabetes, cardiovascular disease and cancer5,6. Consequently, attention is being drawn to the potential for modulation of these changes as an additional approach in the battle against obesity.

The World Health Organisation (WHO) defines probiotic bacteria as ‘live microorganisms that, when administered in adequate amounts confer a health benefit on the host’7, and there is accumulating evidence supporting their impact on the gut microbiota resulting in beneficial effects8,9,10. Recent meta-analyses report that body weight reductions have been achieved in short-term probiotic intervention studies (averaging 2 months) in overweight/obese particpants11,12,13. It is considered that a 3% reduction in bodyweight over a 6–12 month period represents successful weight loss14 whilst current clinical guidelines state that a 5% reduction is required to illicit significant improvements in health15.

In an exploratory placebo-controlled study (PROMAGEN, ISRCTN12562026) the impact of supplementation with the Lab4P probiotic consortium in overweight and obese adults with no dietary or lifestyle restrictions resulted in a significant reduction in body weight (1.5%) over a 6-month period together with improvements in quality-of-life and reductions in the incidence of upper respiratory tract infections16. Stratification of the study population indicated greater weight losses in older participants (2% compared to ≤ 1.2% in younger participants) and with 1.9% weight reduction in overweight participants compared to 1.2% in obese participants16. The design of the current study was based on the findings from the PROMAGEN study. This study’s aim was to repeat the assessment of the PROMAGEN probiotic supplementation specifically in overweight, older participants, measuring body weight (BW), waist circumference (WC), hip circumference (HC) and blood pressure (BP).

Methods

Study approval

This study was a single-centre, double-blind, randomised and placebo-controlled superiority study with equal allocation of participants between two parallel study groups. The study was approved by the Ethical Committee of Comac Medical, Sofia, Bulgaria (Reference: #168/08.05.2019), registered with IRSCTN (ISRCTN18030882, Registration date: 27.11.2019) and was conducted in accordance with the principles of the Declaration of Helsinki.

Recruitment, participants and randomisation

The trial was performed by Comac Medical Ltd and healthy Bulgarian adults (aged 45–65; BMI 25–29.9 kg/m2; WC > 89 cm for women and > 100 cm for men) providing written informed consent were recruited at a trial facility (Sofia, Bulgaria) between 17/05/2019 and 22/05/2019.

Sample size calculations were based on the body weight changes observed in a subgroup of PROMAGEN participants (aged 45–65, BMI 25–29.9 kg/m2) and 31 participants per group were required to detected a 1.54 kg reduction (standard deviation of 2.01 kg) using a Type I error of 0.05 and a power of 85%. Thirty-five participants per group were selected to account for potential drop-outs.

Participants were excluded if they were undergoing immunodeficiency/immunosuppressive therapy; pregnant or planning pregnancy; had history of ischemic heart disease, heart failure, prolonged QTc interval, rhythm and conduction disorders—absolute arrhythmia, ventricular extrasystole, atrioventricular block or any other cardiovascular disease deemed by the investigator as a risk for the participation in the study; had severe systemic disease (cancer, dementia, advanced organ failure); had experienced significant weight loss in the last 3 months that could not be explained by a dietary regimen or increased physical activity; or if they had received any statin therapy in the 6 months prior to the study period.

All participants were sequentially allocated to one of two arms of the study in a 1:1 ratio according to a computer-generated random sequence (block-size of four using SAS PROC PLAN (SAS version 9.4)). An independent statistician provided the randomisation code and the trial product was randomised before arrival at the trial site. The allocation sequence was not made available to researchers until all databases were completed and locked (the allocation sequence was held at the trial site in tamper-proof sealed envelopes in case of emergency).

Study design and intervention

The randomised participants received one capsule per day of either the active Lab4P product or a matching placebo for 9 months (270 days). Participants were advised to take the intervention with food (with or without a cool drink) at any time of day within no less than 2 h of any antibiotic intake. All participants were required to avoid the consumption of any probiotics and maintain their normal diet and lifestyle throughout the study.

The active product (Lab4P) was identical to that used in PROMAGEN16 and comprised Lactobacillus acidophilus CUL60 (NCIMB 30157), Lactobacillus acidophilus CUL21 (NCIMB 30156), Lactobacillus plantarum CUL66 (NCIMB 30280), Bifidobacterium bifidum CUL20 (NCIMB 30153) and Bifidobacterium animalis subsp. lactis CUL34 (NCIMB 30172) at a total of 5 × 1010 colony forming units (cfu) per capsule. The placebo capsules comprised microcrystalline cellulose and were identical in appearance to the active product. All capsules were prepared by Cultech Ltd, Port Talbot, UK and were provided in induction-sealed pots. The trial products were stored between 4 and 8 °C at the trial site and participants were required to refrigerate the intervention throughout the study. Unused capsules were collected for compliance monitoring.

Study outcomes

The primary outcome was change from baseline in body weight (BW). Secondary outcomes were changes from baseline in waist circumference (WC), hip circumference (HC) and blood pressure (BP). Other secondary outcomes are not included in this report.

Data and sample collection

Participants were required to attend the study centre on four occasions: Baseline (day 0), 3 months (day 90 ± 3), 6 months (day 180 ± 3) and 9 months (day 270 ± 3). The height of participants was recorded at baseline and BW, WC, HC and BP were measured at all visits. Pots of 93 capsules were provided to participants at the baseline, 3 and 6 month visits and unused capsules collected at the 3, 6 and 9 month visits.

Physiological measurements

All physiological measurements were made as previously described16. Briefly, BW was recorded using a calibrated column scale (Seca 709, Hamburg, Germany) after the removal of shoes and jackets; WC was measured 2 fingers below the umbilicus; HC was measured at the level of maximum protrusion; seated BP was measured after 5 min respite using a calibrated blood pressure monitor (Omron, Kyoto, Japan) and height was measured after the removal of shoes. The time at which measurements were taken was standardised for each participant wherever possible.

Data management and statistical analysis

Data was analysed as previously described16. Briefly, analysis of study outcomes was performed on an intention-to-treat basis using a linear mixed model (LMM) that included treatment, time and interaction between treatment and time as fixed effects, baseline measurements as the covariate and subject as the random effect. Differences between treatment groups at each time point with 95% confidence intervals (CI) from a t-test were calculated from the LMM. Covariate adjusted analyses within the LMM framework as described above were performed on all outcomes with age, gender and BMI as covariates. Values of p were considered statistically significant when less than 0.05. Continuous variables were summarised using mean ± standard deviation (SD). Data analyses were performed using SAS version 9.4 (SAS Institute Inc., Cary, NC, USA).

Results

Recruitment

Eighty-one conforming candidates were identified from a database of healthy participants and 70 of these participants were recruited. The study took place between May 2019 and February 2020 and there were no drop-outs, exclusions or adverse events in either arm of the study (Fig. 1). Compliance to the intervention (as defined by number of returned capsules) exceeded 99% in both arms of the study.

Figure 1
figure1

Flow diagram of study.

Physiological measurements

Baseline demographics of the participants are shown in Table 1. Changes in BW, BMI, WC and HC are shown in Fig. 2 (detailed data presented in Supplementary Table S1). Significant between group reductions in BW compared to the placebo were seen at 3 months (− 2.51%, − 2.11 kg, p < 0.0001), 6 months (− 2.19%, − 1.88 kg, p < 0.0001) and 9 months where weight loss approached 4% (− 3.76%, − 3.16 kg, p < 0.0001, Fig. 2a). Weight loss from baseline for the probiotic group was consistent and reached significance at 3 months (− 2.83%, − 2.37 kg, p < 0.0001), 6 months (− 4.00%, − 3.35 kg, p < 0.0001) and 9 months (− 4.36%, − 3.65 kg, p < 0.0001). Significant weight loss was recorded in the placebo group at 6 months (− 1.81%, − 1.47 kg, p < 0.0001), but the weight of the participants in the placebo group returned to near baseline by 9 months. Changes in BMI over the course of the study were consistent with weight loss results (Fig. 2b).

Table 1 Demographic and baseline characteristics of the PROBE population.
Figure 2
figure2

Changes from baseline in (a) body weight, (b) BMI, (c) waist circumference and (d) hip circumference over the duration of the intervention period. Data is presented as mean change from baseline (35 participants per group) with 95% CI and p values calculated using a LMM. For within group comparisons (vs. baseline): **p ≤ 0.01 and ***p ≤ 0.001. For between group comparisons (active vs. placebo): ###p ≤ 0.001.

The weight loss was reflected in significant between group reductions in both WC and HC in response to the probiotic at each time point and by 9 months had dropped by 2.48% (− 2.58 cm, p < 0.0001, Fig. 2c) for WC and 2.36% (− 2.66 cm, p < 0.0001, Fig. 2d) for HC. These changes contributed to significant reductions in the waist-to-height ratio (WtHR) and the conicity index \(({\text{WC}}\;({\text{cm}}){/}(0.109\surd {\text{BW}}({\text{kg}}){\text{/Height}}\;({\text{m}})))\)17 in the probiotic group (Supplementary Table S1). No changes in systolic or diastolic blood pressure were recorded during the study.

Rates of successful weight loss (SWL) and clinical weight loss (CWL)

Figure 3 clearly indicates consistency of the weight loss in the active group with 33/35 participants achieving weight loss by 9 months (Fig. 3a). In the placebo group there was little weight loss in the first 6 months, and then weight regain at 9 months (Fig. 3b). At the end of the study, six participants from the active group moved from overweight BMI status to healthy BMI status (< 24.9 kg/m2) compared with two participants in the placebo group (Fig. 3c). Three participants in the placebo group progressed into the obese BMI status (≥ 30 kg/m2) but none in the active group.

Figure 3
figure3

Absolute changes in body weight for each participant in the (a) active group or (b) placebo group over the duration of the study and the distribution of changes in participant (c) BMI and (d) percentage change in body weight. Data is presented for 35 participants per group.

The distribution of percentage weight change can be seen in Fig. 3d. In the early stages of the study all participants lost or maintained weight (except one in the placebo group) with greater percentage weight losses in the active group. A weight loss of ≥ 3% (considered “successful” weight loss by Grembi et al.14) was achieved by 17/35 active group participants and 0/35 placebo group participants after 3 months supplementation (49% vs. 0% respectively, p < 0.0001, Figs. 3d, 4). By 9 months more than 70% of the active group had achieved 3% weight loss compared with less than 20% of the placebo group (71% vs. 17% respectively, p < 0.0001).

Figure 4
figure4

The number of participants achieving successful weight loss and clinical weight loss after 3 months, 6 months and 9 months. Data is presented for 35 participants per group. SWL successful weight loss, CWL clinical weight loss. Diagram created using sankeyMATIC (http://sankeymatic.com/faq/).

In both groups there were instances where weight loss had reached levels that could be considered clinically meaningful (defined as 5% weight loss over a 6–12 month period15) and these reductions were more prevalent in the active group compared to the placebo at both 6 months (31% vs. 6%, p = 0.0071) and 9 months (40% vs. 3%, p = 0.0002).

Discussion

Daily supplementation of a free-living population of healthy overweight individuals aged 45–65 with the Lab4P probiotic for 9 months resulted in a significant reduction in body weight of 3.8% together with a 2.5% reduction in waist circumference and a 2.4% reduction in hip circumference. No significant changes in blood pressure were detected.

In our previous 6-month exploratory study assessing the impact of the Lab4P probiotic (PROMAGEN) in free-living overweight and obese adults, weight-loss was identified as a significant outcome16. At 6 months, significant body weight reductions were seen in the probiotic treated group compared to the placebo (− 1.30 kg, 95% CI − 1.77, − 0.83, p < 0.0001) and sub-group analysis of the population revealed the highest weight losses in participants aged 50 or over (2% reduction, p = 0.0002) or in the overweight category (1.9% reduction, p < 0.0001)16. In the meta-analysis of Koutnikova et al., greater levels of probiotic-mediated weight loss were observed in overweight participants compared to the obese11. Although much attention is focused on obese individuals, it is estimated that about 70% of adults in England aged between 45 and 65 are classified as overweight18 and these people need support to prevent their progression into an obese state due to the anticipated annual weight gain3.

PROBE was designed as the follow-up to the PROMAGEN study with the aim of confirming the probiotic-associated weight losses observed in overweight adults16 and significant reductions in body weight occurred in the absence of any restrictions imposed on daily diet or lifestyle. Studies demonstrating consistent probiotic-mediated weight loss are extremely rare with existing reports limited to Asian populations and the results of these are variable19,20,21,22. Numerous meta-analyses have attempted to estimate the overall probiotic weight loss effect from studies with highly variable designs (many with lifestyle/dietary restrictions), interventions (strain and dose) and target populations and have reported reductions in the region of 0.5–1%11,23 or no effect12,24. We are one of the first groups to demonstrate a consistent weight loss effect across two studies in free-living western populations.

The intervention period in the PROBE study was extended to 9 months to identify if the weight changes observed over the 6-month period in the PROMAGEN study might be on-going. We found that weight loss continued up to 9 months at which point the between group difference in body weight approached 3.2 kg (~ 7 lb). Clinically meaningful levels of weight loss are defined as a 5% reduction in body weight over a 6–12 month period and are associated with measurable metabolic benefits (such as improvements in glycaemic index and plasma lipid levels) and reduced healthcare costs15,25. Using ≥ 5% weight loss as a potentially beneficial reference point, 40% of the Lab4P treated group reached this “clinical” target after 9 months supplementation compared to only 3% of participants in the placebo group.

In both the PROBE and PROMAGEN studies, the intervention period spanned Christmas and New Year and weight gains of 0.4–1.0 kg have been observed in the general population over this period3. In the PROBE study, the placebo group gained weight during this period (between 6 to 9 months), and this gain counterbalanced the overall weight losses observed in the first 6 months. However, modest weight loss was maintained in the probiotic group through this period. This difference raises the possibility that Lab4P supplementation may help prevent the weight regain observed in the placebo group after weight loss that is a near-ubiquitous problem driven by physiological, psychological and environmental factors26. It has been found that more than half of the weight lost by an individual is regained within 2 years of the initial weight loss27.

The PROBE study reiterated the observed reduction in waist circumference in the PROMAGEN study and also demonstrated a reduction of hip circumference and the waist/hip ratio. These criteria are used to assess abdominal (central) obesity and elevated waist/hip measurements, and have been linked to the development of cardiovascular disease28,29 and dementia30.

Numerous mechanisms of action for probiotic-mediated weight loss have been proposed. These include the deconjugation of bile acids by bacterial bile salt hydrolases (BSH) which has been shown to impact on a number of key physiological processes in the host including lipid metabolism31,32. Studies in vitro with the Lab4P probiotics indicate that this consortium expresses BSH activity33,34 and functionality was confirmed in mouse studies where Lab4P treated C57BL/6 mice had increased faecal levels of deconjugated bile acids that occurred alongside the inhibition of high fat diet induced weight gain34. In the PROMAGEN study with adults, an association between the weight loss and high baseline faecal levels of sulphated bile acids was identified, leading us to conclude that host physiology may also be a contributory factor influencing the probiotic associated weight loss35.

The strengths of our study include the powered sample size, the 9-month intervention period and the unadjusted lifestyle conditions, that give our findings applicability to the ‘real world’ setting. The limitations of our study include the initial weight loss observed in the placebo group although this did not persist over the entirety of our study and the placebo effect has become a recognised feature of obesity research36. The specific BMI range and single geographical isolation of our study population provides uniformity and continuity on one hand but requires further work in both obese and ideal weight participants and in another setting.

In summary, the PROBE intervention study has demonstrated that 9 months Lab4P supplementation at 5 × 1010 cfu/day significantly reduced bodyweight, BMI, waist circumference and hip circumference in a free-living overweight population aged 45–65 years old. These results agreed with the outcomes a previous exploratory study and highlight the potential for a role for probiotic supplementation within the weight management arena.

Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

References

  1. 1.

    Valdes, A. M., Walter, J., Segal, E. & Spector, T. D. Role of the gut microbiota in nutrition and health. BMJ 361, k2179. https://doi.org/10.1136/bmj.k2179 (2018).

    Article  PubMed  PubMed Central  Google Scholar 

  2. 2.

    de Vos, W. M. & de Vos, E. A. Role of the intestinal microbiome in health and disease: from correlation to causation. Nutr. Rev. 70(Suppl 1), S45–S56. https://doi.org/10.1111/j.1753-4887.2012.00505.x (2012).

    Article  PubMed  Google Scholar 

  3. 3.

    Turicchi, J. et al. Weekly, seasonal and holiday body weight fluctuation patterns among individuals engaged in a European multi-centre behavioural weight loss maintenance intervention. PLoS ONE 15, e0232152. https://doi.org/10.1371/journal.pone.0232152 (2020).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Ponti, F. et al. Aging and imaging assessment of body composition: from fat to facts. Front. Endocrinol. (Lausanne) 10, 861. https://doi.org/10.3389/fendo.2019.00861 (2019).

    Article  Google Scholar 

  5. 5.

    WHO. World Health Organisation, Fact Sheet No. 311: Obesity and Overweight (2018).

  6. 6.

    Dobbs, R. et al. Overcoming obesity: an initial economic assessment. A discussion paper by the McKinsey Global Institute (2014).

  7. 7.

    Hill, C. et al. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat. Rev. Gastroenterol. Hepatol. 11, 506–514. https://doi.org/10.1038/nrgastro.2014.66 (2014).

    Article  PubMed  Google Scholar 

  8. 8.

    Liu, Y., Tran, D. Q. & Rhoads, J. M. Probiotics in disease prevention and treatment. J. Clin. Pharmacol. 58(Suppl 10), S164–S179. https://doi.org/10.1002/jcph.1121 (2018).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Abenavoli, L. et al. Gut microbiota and obesity: a role for probiotics. Nutrients https://doi.org/10.3390/nu11112690 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Aoun, A., Darwish, F. & Hamod, N. The influence of the gut microbiome on obesity in adults and the role of probiotics, prebiotics, and synbiotics for weight loss. Prev. Nutr. Food Sci. 25, 113–123. https://doi.org/10.3746/pnf.2020.25.2.113 (2020).

    Article  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Koutnikova, H. et al. Impact of bacterial probiotics on obesity, diabetes and non-alcoholic fatty liver disease related variables: a systematic review and meta-analysis of randomised controlled trials. BMJ Open 9, e017995. https://doi.org/10.1136/bmjopen-2017-017995 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  12. 12.

    Suzumura, E. A. et al. Effects of oral supplementation with probiotics or synbiotics in overweight and obese adults: a systematic review and meta-analyses of randomized trials. Nutr. Rev. https://doi.org/10.1093/nutrit/nuz001 (2019).

    Article  PubMed  Google Scholar 

  13. 13.

    Wang, Z.-B. et al. The potential role of probiotics in controlling overweight/obesity and associated metabolic parameters in adults: a systematic review and meta-analysis. Evidence-Based Complement. Altern. Med. https://doi.org/10.1155/2019/3862971 (2019).

    Article  Google Scholar 

  14. 14.

    Grembi, J. A. et al. Gut microbiota plasticity is correlated with sustained weight loss on a low-carb or low-fat dietary intervention. Sci. Rep. 10, 1405. https://doi.org/10.1038/s41598-020-58000-y (2020).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. 15.

    Jensen, M. D. et al. 2013 AHA/ACC/TOS guideline for the management of overweight and obesity in adults. Circulation 129, S102–S138. https://doi.org/10.1161/01.cir.0000437739.71477.ee (2014).

    Article  PubMed  Google Scholar 

  16. 16.

    Michael, D. R. et al. A randomised controlled study shows supplementation of overweight and obese adults with lactobacilli and bifidobacteria reduces bodyweight and improves well-being. Sci. Rep. 10, 4183. https://doi.org/10.1038/s41598-020-60991-7 (2020).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  17. 17.

    Motamed, N. et al. Conicity index and waist-to-hip ratio are superior obesity indices in predicting 10-year cardiovascular risk among men and women. Clin. Cardiol. 38, 527–534. https://doi.org/10.1002/clc.22437 (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  18. 18.

    NHS Digital: Statistics on Obesity, Physical Activity and Diet, England. https://digital.nhs.uk/data-and-information/publications/statistical/statistics-on-obesity-physical-activity-and-diet/statistics-on-obesity-physical-activity-and-diet-england-2019 (2019).

  19. 19.

    Minami, J. et al. Effects of Bifidobacterium breve B-3 on body fat reductions in pre-obese adults: a randomized, double-blind, placebo-controlled trial. Biosci. Microbiota Food Health 37, 67–75. https://doi.org/10.12938/bmfh.18-001 (2018).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. 20.

    Minami, J. et al. Oral administration of Bifidobacterium breve B-3 modifies metabolic functions in adults with obese tendencies in a randomised controlled trial. J. Nutr. Sci. 4, e17. https://doi.org/10.1017/jns.2015.5 (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Kadooka, Y. et al. Effect of Lactobacillusgasseri SBT2055 in fermented milk on abdominal adiposity in adults in a randomised controlled trial. Br. J. Nutr. 110, 1696–1703. https://doi.org/10.1017/S0007114513001037 (2013).

    CAS  Article  PubMed  Google Scholar 

  22. 22.

    Kadooka, Y. et al. Regulation of abdominal adiposity by probiotics (Lactobacillusgasseri SBT2055) in adults with obese tendencies in a randomized controlled trial. Eur. J. Clin. Nutr. 64, 636–643. https://doi.org/10.1038/ejcn.2010.19 (2010).

    CAS  Article  PubMed  Google Scholar 

  23. 23.

    Wang, L. et al. The effects of probiotics on total cholesterol: a meta-analysis of randomized controlled trials. Medicine (Baltimore) 97, e9679. https://doi.org/10.1097/MD.0000000000009679 (2018).

    CAS  Article  Google Scholar 

  24. 24.

    Dong, Y., Xu, M., Chen, L. & Bhochhibhoya, A. Probiotic foods and supplements interventions for metabolic syndromes: a systematic review and meta-analysis of recent clinical trials. Ann. Nutr. Metab. 74, 224–241. https://doi.org/10.1159/000499028 (2019).

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Ryan, D. H. & Yockey, S. R. Weight loss and improvement in comorbidity: differences at 5%, 10%, 15%, and over. Curr. Obes. Rep. 6, 187–194. https://doi.org/10.1007/s13679-017-0262-y (2017).

    Article  PubMed  PubMed Central  Google Scholar 

  26. 26.

    Hall, K. D. & Kahan, S. Maintenance of lost weight and long-term management of obesity. Med. Clin. N. Am. 102, 183–197. https://doi.org/10.1016/j.mcna.2017.08.012 (2018).

    Article  PubMed  Google Scholar 

  27. 27.

    Anderson, J. W., Konz, E. C., Frederich, R. C. & Wood, C. L. Long-term weight-loss maintenance: a meta-analysis of US studies. Am. J. Clin. Nutr. 74, 579–584. https://doi.org/10.1093/ajcn/74.5.579 (2001).

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Cao, Q. et al. Waist-hip ratio as a predictor of myocardial infarction risk: a systematic review and meta-analysis. Medicine (Baltimore) 97, e11639. https://doi.org/10.1097/MD.0000000000011639 (2018).

    Article  Google Scholar 

  29. 29.

    Huxley, R., Mendis, S., Zheleznyakov, E., Reddy, S. & Chan, J. Body mass index, waist circumference and waist:hip ratio as predictors of cardiovascular risk—a review of the literature. Eur. J. Clin. Nutr. 64, 16–22. https://doi.org/10.1038/ejcn.2009.68 (2010).

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    Ma, Y., Ajnakina, O., Steptoe, A. & Cadar, D. Higher risk of dementia in English older individuals who are overweight or obese. Int. J. Epidemiol. https://doi.org/10.1093/ije/dyaa099 (2020).

    Article  PubMed  PubMed Central  Google Scholar 

  31. 31.

    Cerdo, T., Garcia-Santos, J. A., Mercedes, G. B. & Campoy, C. The role of probiotics and prebiotics in the prevention and treatment of obesity. Nutrients https://doi.org/10.3390/nu11030635 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  32. 32.

    Joyce, S. A. et al. Regulation of host weight gain and lipid metabolism by bacterial bile acid modification in the gut. Proc. Natl. Acad. Sci. U.S.A. 111, 7421–7426. https://doi.org/10.1073/pnas.1323599111 (2014).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  33. 33.

    Michael, D. et al. Lactobacillusplantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes. Benef. Microbes 7, 443–451 (2016).

    CAS  Article  Google Scholar 

  34. 34.

    Michael, D. R. et al. The anti-cholesterolaemic effect of a consortium of probiotics: an acute study in C57BL/6J mice. Sci. Rep. 7, 2883. https://doi.org/10.1038/s41598-017-02889-5 (2017).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  35. 35.

    Mullish, B. H. et al. Identifying the factors influencing outcome in probiotic studies in overweight and obese patients: host or microbiome?. Gut https://doi.org/10.1136/gutjnl-2020-321110 (2020).

    Article  PubMed  PubMed Central  Google Scholar 

  36. 36.

    Fontaine, K. R., Williams, M. S., Hoenemeyer, T. W., Kaptchuk, T. J. & Dutton, G. R. Placebo effects in obesity research. Obesity (Silver Spring) 24, 769–771. https://doi.org/10.1002/oby.21456 (2016).

    Article  Google Scholar 

Download references

Acknowledgements

This study was funded by Cultech limited and received no specific grant from any funding agency in the public, commercial or not-for-profit sectors. BHM is the recipient of a National Institute of Health Research (NIHR) Academic Clinical Lectureship. The Division of Digestive Diseases at Imperial College London, BHM and JRM receive financial support from the NIHR Imperial Biomedical Research Centre (BRC) based at Imperial College Healthcare NHS Trust and Imperial College London. The views expressed in this publication are those of the authors and not necessarily those of the NHS, NIHR, or the Department of Health.

Author information

Affiliations

Authors

Contributions

DRM, TSD and SFP were responsible for the design of the study and preparation of the manuscript. AAJ and GM performed data analysis and DW performed statistical analysis. JRM and BHM provided guidance that was critical to the completion of the study. All the authors contributed to the review of the manuscript.

Corresponding author

Correspondence to D. R. Michael.

Ethics declarations

Competing interests

DRM, TSD, AAJ, GM and SFP are/were employees of Cultech Ltd. JRM, BHM and DW are have been involved in other collaborative projects with Cultech Ltd.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Michael, D.R., Davies, T.S., Jack, A.A. et al. Daily supplementation with the Lab4P probiotic consortium induces significant weight loss in overweight adults. Sci Rep 11, 5 (2021). https://doi.org/10.1038/s41598-020-78285-3

Download citation

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing