Reverse western blot assays and cell migrations. Reverse western blot assays were performed using human angiogenesis antibody arrays (R&D systems), which detected the relative quantities of 55 cytokines in EGM-2 (a) and HLFCM (b). Representative images of the cytokine array membranes. Factors in red rectangles represent pro-angiogenic growth factors. Factors in blue rectangles represent anti-angiogenic growth factors. (c) The effect of pro-angiogenetic growth factors on HUVECs migration in collagen I coated Platypus migration plates was tested. The migration of HUVECs in EBM-2 (top left) and EGM-2 (bottom left) media as well as EGM-2 media supplemented with VEGF (top middle), PIGF (top right), HB-EGF (bottom middle), and IL-8 (bottom right) pro-angiogenetic growth factors were examined. (d) Fluorescence intensity for combinations test of the four pro-angiogenetic growth factors.