Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

A natriuretic peptide from Arabidopsis thaliana (AtPNP-A) can modulate catalase 2 activity


Analogues of vertebrate natriuretic peptides (NPs) present in plants, termed plant natriuretic peptides (PNPs), comprise a novel class of hormones that systemically affect salt and water balance and responses to plant pathogens. Several lines of evidence indicate that Arabidopsis thaliana PNP (AtPNP-A) affects cellular redox homeostasis, which is also typical for the signaling of its vertebrate analogues, but the molecular mechanism(s) of this effect remains elusive. Here we report identification of catalase 2 (CAT2), an antioxidant enzyme, as an interactor of AtPNP-A. The full-length AtPNP-A recombinant protein and the biologically active fragment of AtPNP-A bind specifically to CAT2 in surface plasmon resonance (SPR) analyses, while a biologically inactive scrambled peptide does not. In vivo bimolecular fluorescence complementation (BiFC) showed that CAT2 interacts with AtPNP-A in chloroplasts. Furthermore, CAT2 activity is lower in homozygous atpnp-a knockdown compared with wild type plants, and atpnp-a knockdown plants phenocopy CAT2-deficient plants in their sensitivity to elevated H2O2, which is consistent with a direct modulatory effect of the PNP on the activity of CAT2 and hence H2O2 homeostasis. Our work underlines the critical role of AtPNP-A in modulating the activity of CAT2 and highlights a mechanism of fine-tuning plant responses to adverse conditions by PNPs.


In plants abiotic and biotic stress triggers highly complex stimulus-specific cellular and systemic signals and responses consisting of many different components including receptors, sensors, plant hormones, notably abscisic acid (ABA), jasmonic acid and (second) messengers such as calcium and cyclic mononucleotides1,2,3,4. Perception of many phytohormones by different types of intracellular and extracellular receptors is well documented. For instance, ABA is not only perceived by a family of nucleocytoplasmic PYR/PYL/RCAR (PYLs) that bear unequivocal hallmarks of the bona fide ABA receptors5,6, but also by plasma membrane guard cell outward rectifying potassium channel (GORK)7, and possibly other receptors of ABA are still to be discovered.

Similarly, identification of novel receptors targeted by an increasing number of peptidic plant hormones discovered in the last few decades8,9 is anticipated. One of those, the plant natriuretic peptides (PNPs), are a group of systemically mobile signals10 that have a role in the maintenance of salt and water balance11,12. An A. thaliana PNP, termed AtPNP-A (At2g18660; Q9ZV52), has been implicated in several physiological processes ranging from the regulation of stomatal aperture13, osmoticum-dependent volume changes13,14 and modulation of developmental stage- and tissue-specific ion fluxes15, to immune responses16,17. Much like vertebrate natriuretic peptides (NPs)18, many of the effects elicited by AtPNP-A involve rapid elevation of 3′,5′-cyclic guanosine monophosphate (cGMP)13,19, and increasing evidence suggests that reactive oxygen species (ROS) are also secondary messengers in the transduction of AtPNP-A signals20. Nevertheless, understanding of the molecular mechanisms by which PNPs exert their functions is limited by lack of comprehensive studies reporting sets of proteins that interact with PNPs to modulate levels of secondary messengers, including cGMP or ROS, which are relevant elements of plant defense responses regulated by PNPs. Although at least two receptors perceiving AtPNP-A have been identified16,21, and at least one of them catalyzes generation of cGMP upon binding of AtPNP-A21, determination of the signaling underlying AtPNP-A-dependent modulation of ROS has not been attempted. In particular, abiotic and biotic stress can cause rapid increases in hydrogen peroxide (H2O2) levels which in turn activate physiological responses, including seed germination22,23, stomatal aperture movement regulation24,25,26,27, programmed cell death28,29 and others30. Many of these events are known to be modulated by PNPs10,13,16,17,20,31,32. Since these processes have implications on salt stress acclimation33, 34, drought tolerance35,36, and plant responses to other abiotic37 and biotic38 stresses, understanding how PNPs affect ROS could enhance elucidation of plant stress responses in agricultural settings.

In this study, we set out to identify interactors of AtPNP-A to elucidate possible direct links between the hormone and changes in cellular redox homeostasis and identified Catalase 2 (CAT2; At4g35090; P25819) as a direct binding partner of AtPNP-A. CAT2 has been reported as the major enzyme involved in detoxifying ROS in the photosynthetic tissues39 essential for optimal development of C3 plants grown in air40. Using a series of biochemical and physiological approaches we investigated the specificity of this binary interaction and its biological relevance. Synthetic peptide containing amino acids corresponding to the evolutionarily conserved active site of AtPNP-A binds specifically not only to A. thaliana CAT2, but also to animal-derived catalase, underlying general significance of the interaction between NPs and catalases in different kingdoms. We show that AtPNP-A modulates enzymatic activity of CAT2, the interaction occurs in chloroplasts, and atpnp-a knockdown mutant displays differential responses to redox stress, phenocopying cat2 knockout mutant plants. The interaction between PNPs and CAT2 affects cellular H2O2, thereby possibly modulating biotic and abiotic stress responses.


Plant materials and growth conditions

Seeds of Arabidopsis thaliana (Col-0) and mutants carrying T-DNA insertion in either CAT2 (cat2-2; SALK_057998) or AtPNP-A (atpnp-a; SALK_000951)41, from the European Arabidopsis Stock Centre (uNASC;, were surface-sterilized and vernalized, sown in Jiffy peat pellets and grown at 23 °C in 16 h of light (200 μmol s−1 m−2) per day or on Murashige-Skoog agar plates and grown at 23 °C in 16 h of light (100 μmol s−1 m−2) per day for 10 days. Plant genotyping is detailed in Supplementary Table S1. Determination of the site of the T-DNA insertion in the atpnp-a homozygous mutant line was done by sequencing of the purified PCR fragment containing the junction of the T-DNA and plant genomic DNA obtained with the use of the mutant allele-specific primers given in Supplementary Table S1. Nicotiana tabacum seeds were germinated on soil (Debco seed raising mix:vermiculite, 3:1). At three weeks seedlings were transferred to three plants per pot (Debco soil mix:vermiculite, 3:1). Plants were grown under long day conditions (16 h light, 8 h dark) at 22 °C.

Amino acid sequence alignment

Amino acids sequences of A. thaliana AtPNP-A (NP_849979.1) orthologs expressed in S. tuberosum (StPNP-A; XP_015162511.1) and in N. tabaccum (NtPNP-A; XP_016448760) were derived from NCBI with pBLAST search using AtPNP-A sequence without the signal peptide (119–130) as a query. The alignment was performed using ClustalW software.

Transmission electron microscopy (TEM)

Samples for transmission electron microscopy (TEM) were prepared according to42. Briefly, 2 mm sections of potato were plunged into liquid propane pre-cooled on liquid nitrogen at approximately − 200 °C. Samples were freeze substituted for 3 weeks at − 70 °C in 1% (w/v) glutaraldehyde in 2,2-dimethoxypropane, acetone and methanol. Upon bringing to room temperature, the samples were rinsed in solvent and infiltrated with LR Gold resin over a further 2 weeks. Samples were then polymerized at room temperature with a catalyst, and ultrathin sections were cut on an ultramicrotome using a diamond knife. Sections (80 nm thick) were picked up on pioloform coated gold grids and dried. These grids were immune-stained, upon BSA/PBS blocking, with the polyclonal anti-human ANP antibody43, followed by goat anti-rabbit antibody pre-conjugated to 40 nm gold nanoparticles. Grids were examined in a transmission electron microscope (Phillips). Photos were captured on negatives and processed in the dark room, printed, and scanned to digital. If more contrast was then needed to examine cell details, the grids were post-stained in uranyl acetate and lead citrate. Controls for immunolabeling included omission of antibody and usage of an irrelevant antibody (monoclonal antibody directed against grass allergens).

Verification of biological activity of synthetic peptides

Peptides containing amino acid sequence of the active region of AtPNP-A and corresponding scrambled peptides, having the same amino acid composition but in a randomized order (Fig. 1a), with or without C-terminal biotin tag, were synthesized by GenScript (Piscataway, USA) at the purity level > 95% verified with HPLC and their biological activity was verified as described previously21.

Figure 1

Biologically active peptide containing the active region of AtPNP-A protein binds to CAT2. (a) Domain organization of AtPNP-A and the amino acid sequences of C-terminally biotinylated peptides used in affinity chromatography-based experiments—a peptide containing the active site of AtPNP-A (indicated as pAtPNP-A) or the corresponding scrambled peptide (indicated as pScr). Cysteine residues forming a disulfide bond, characteristic to natriuretic peptide (NP)-like molecules, are underlined. SP, signal peptide. (b) Assaying biological activity of the AtPNP-A peptide (pAtPNP-A) and purified recombinant protein (rAtPNP-A). A. thaliana (Col-0) mesophyll cell protoplasts suspended in 0.4 M mannitol were treated with either water or 100 nM pScr (negative controls) or with 100 nM pAtPNP-A or with 1 μg mL−1 of rAtPNP-A protein for 20 min at room temperature. In each treatment, 50 randomly selected protoplasts with diameter > 20 µm were included in quantitative analysis (scale bar = 20 µm). Protoplast volume was measured and the data obtained from an exemplar experiment are plotted. Columns with different superscript (a and b) indicate significantly different results (mean ± SD, one way ANOVA followed by Tukey–Kramer multiple comparison test, n = 50, P < 0.0001). (c) Exemplar MS/MS spectrum of a unique tryptic peptide of CAT2 (At4g35090) protein.

Identification of AtPNP-A interactors by protein-peptide cross-linking followed by affinity-based isolation and LC–MS/MS analysis

Cross-linking experiments followed by affinity-based isolation and LC–MS/MS identification of AtPNP-A interactors were performed on A. thaliana (Col-0) wild type (WT) mesophyll cell protoplasts (MCPs) as described in44.

Identification of AtPNP-A interactants by yeast two-hybrid (Y2H) screen

The Y2H screen using AtPNP-A as bait was carried out by Dualsystems Biotech AG (Zurich, Switzerland). The bait construct for the screening was made by subcloning the cDNA fragment encoding exported portion of AtPNP-A (amino acids 26 to 130) (Fig. 1a) into the pLexA-DIR vector (Dualsystems Biotech AG). The bait construct was transformed into the strain NMY32 (MATa his3Δ200 trp1-901 leu2-3,112 (lexAop)8-ADE2 LYS2::(lexAop)4-HIS3 URA3::(lexAop)8-lacZ GAL4) using standard procedures45. Correct expression of the bait was verified by western blotting of cell extracts using a mouse monoclonal antibody directed against the LexA domain (Dualsystems Biotech AG). The absence of self-activation was verified by co-transformation of the bait together with a control prey and selection on minimal medium lacking tryptophan, leucine, and histidine (selective medium). For the Y2H screen, the bait was co-transformed together with a normalized A. thaliana universal P02403 cDNA library (Dualsystems Biotech AG) into NMY32. 3.1 × 106 transformants were screened, yielding 96 transformants that grew on selective medium. Positive transformants were tested for β-galactosidase assay and only those that showed β-galactosidase activity were considered true positives. Library plasmids were isolated from positive clones. The identity of positive interactants was determined by sequencing. DNA sequences translated in all three reading frames and searching against the SwissProt database using the BLASTX algorithm (

Association of AtPNP-A with chloroplasts

Protoplasts were isolated from Arabidopsis leaves, transiently transfected with the full-length AtPNP-A (GFP:signal peptide AtPNP-A fusion expression vector)46 and imaged with a LSM Pascal confocal microscope (Zeiss). Green channel (FITC) and red channel (TR) were selected together (multi tracks) for scanning. Images were stored and viewed in the LSM Image Browser software (Zeiss). A crude chloroplast preparation was obtained from WT Arabidopsis leaves according to47. Proteins from chloroplasts and WT untransfected protoplasts were extracted as previously described in46 and protein concentrations were measured using the Quant-iT protein assay kit (Invitrogen). Western analysis was according to a standard protocol48. The primary anti-AtPNP antibody was prepared against peptides representing amino acids 44–55 of AtPNP-A as described in49. Immunoreactive bands were visualized by incubating with TM/B peroxidase substrate solution (Chemicon/Millipore) for about 5 min.

Oxidative burst measurement

Leaf discs (0.196 cm2) of 4-week-old WT plants were incubated overnight floating on 0.1 mL water in 96-well titer plate, with one disc per well. The following day the leaf discs were pre-treated with 100 nM rAtPNP-A or mock for 30 min. For ROS detection horseradish peroxidase and luminol (Sigma-Aldrich) were added to a final concentration of 10 μg mL−1 and 100 μM, respectively. Luminescence was measured directly after addition of either 1 μM of flg22 or 100 nM rAtPNP-A in a MicroLumat LB96P plate reader (Berthold Technologies) for 1 h and is shown in relative light units.

Bimolecular fluorescence complementation (BiFC) of AtPNP-A and CAT2

The full-length A. thaliana AtPNP-A (At2g18660) coding region entry plasmid pENTRYAtPNP-A46 and pCR8/GW-TOPOCAT2 were recombined separately with both pSITE-nEYFP-C1 [GenBank Accession Number GU734651] and pSITE-cEYFP-C1 [GenBank Accession Number GU734652] to create pNeYFP-AtPNP-A, pCeYFP-AtPNP-A, pNeYFP-CAT2 and pCeYFP-CAT2, respectively. Each plasmid has either the N-terminal (NeYFP) or C-terminal (CeYFP) fragment of eYFP followed by either AtPNP-A or CAT2 coding region driven by the 35S plant expression promoter. After sequence was confirmed each plasmid was transformed separately into Agrobacterium tumefaciens strain GV3101 (pMP90). Tobacco leaves were infiltrated as previously described in50. Briefly, A. tumefaciens strains grown in 2YT medium were harvested by centrifugation and resuspended in infiltration medium (10 mM MES pH 5.6, 10 mM MgCl2, 150 µM acetosyringone) to a final OD600 of 0.2. Equal volumes of infiltration media containing relevant BiFC strains were mixed and infiltrated into the underside of tobacco leaves using a needleless syringe. Leaves were harvested after 3 days to isolate protoplasts following51 and then immediately imaged.

BiFC images were captured at the La Trobe University LIMS BioImaging Facility using a LSM 510 confocal laser scanning microscope (Zeiss), with a × 40/1.2 water immersion objective. The natural red autofluorescence of chlorophyll was used as a marker for chloroplasts52. Excitation/emission wavelengths were 488/505–530 nm for YFP and 561/650 nm long pass for red fluorescence. At least three images containing one or more protoplasts were taken for each of the plasmid construct combinations. Each image captured three separate filter scans, including YFP, red fluorescence and bright field. Post-processing of images was performed with ImageJ version 1.48 software ( For each image the contrast for each panel was set at automatic contrast. A merged image was generated for reference with a 10 µm scale bar. The red fluorescence only panel was used to encircle ten chloroplasts as region of interest (ROI), then the YFP fluorescence (BiFC) calculated for each ROI was divided by the red fluorescence calculated for the same ROI. All BiFC/red fluorescence points were plotted using GraphPadPrism7 (GraphPadSoftware). Mean and standard error were calculated for each BiFC combination. All statistical analyses were performed using GraphPadPrism7.

Prediction of protein associations and protein–protein docking

The structures of AtPNP-A as well as CAT2 monomer were predicted using the iterative threading assembly refinement (I-TASSER; method53. Protein–protein docking was performed using ClusPro (version 2.0; The models were analyzed and visualized using UCSF Chimera (version 1.10.2)55.

Preparation of recombinant proteins

N-terminally 6xHis-tagged AtPNP-A and CAT2 proteins were expressed in BL21 (DE3) One Shot E. coli cells (Life Technologies), purified by affinity chromatography with Ni–NTA beads (Qiagen) and HisTrap HP column (GE Healthcare Lifesciences) as described in21. The purity of preparations was verified on 12.5% SDS-PAGE, stained with Coomassie Brilliant Blue (Bio-Rad). Protein identities were confirmed in MS analysis, and protein concentration was determined according to the method of Bradford using BSA as a standard.

Surface plasmon resonance (SPR) analyses

SPR experiments were performed at 20 °C on Biacore T100 instrument operated using Biacore T100 control software (version 2.0.2, GE Healthcare Lifesciences) using Series S CM5 or NTA sensor chips as described in21. Kinetic analyses were performed at the flow of 100 μL min−1 with pAtPNP-A (at 3.78 μM and consecutive twofold dilutions; 11 injections included) employed as a ligand and bovine liver CAT (C-40; Sigma) used as an analyte and immobilized on the active surface of the Series S CM5 sensor chip with amine coupling kit, while the surface regeneration was done with solution of glycine, pH 2.0, and the final sensorgram was generated with Scrubber (BioLogic Software Pty Ltd).

Isothermal titration calorimetry (ITC)

ITC experiments were undertaken using MicroCal iTC200 (Malvern Panalytical) calorimeter. The titrations were conducted at 25 °C in PBS buffer at pH 7.4, with 100 μM pAtPNP-A used as a titrant. Total number of injections was set at 30, reference power was 11 μcal s−1, initial delay was 60 s, and stirring speed was 750 rpm.

Detection of CAT activities and isozymes analysis

Enzymatic activity of CAT isozymes and total CAT activities of protein extracted from WT and atpnp-a or cat2-2 mutant lines, when indicated sprayed with 1 nM pAtPNP-A or pScr 20 min before harvesting, were determined zymographically and spectrophotometrically, as described in56. Relative quantification of intensity of bands corresponding to different CAT isoforms was performed with ImageJ version 1.48 ( Enzymatic activity of the rCAT2 in the presence of absence of 100 nM rAtPNP-A, pAtPNP-A or pScr were determined with the use of Amplex Red catalase assay kit (Invitrogen) according to the manufacturer’s instructions.

Germination assay

Screening for H2O2 tolerance of cat2-2, atpnp-a, and WT, the seeds were germinated on Murashige-Skoog agar plates supplemented with 1% (w/v) sucrose and 3 mM H2O2. The plates were incubated in growth chambers under conditions described above and photographed on day 10. Each assay was performed at least three times in triplicate, with 50 seeds sown per each line, and the results of three independent experiments per treatment (mean ± SD) were plotted.


Identification of CAT2 as a candidate interactor of AtPNP-A

We sought biologically relevant binding partners of AtPNP-A using two complementary approaches to identify in vivo protein–protein interactions, namely protein-peptide cross-linking followed by affinity-based isolation and liquid chromatography-tandem mass spectrometry (LC–MS/MS) analysis as well as yeast two-hybrid (Y2H) screen. Since common organellar localization of interacting proteins is a pre-requisite for the binding event to occur, we first verified subcellular localization of PNPs (Supplementary Fig. S1b–e). Initially, we examined subcellular localization of PNP, termed StPNP, natively expressed in potato (Solanum tuberosum), due to the large cell size and responsiveness of this system to both atrial natriuretic peptide (ANP) and immunoreactive plant PNPs19, as the active region of StPNP is highly conserved (Supplementary Fig. S1a). Electron microscopy suggested that PNP localizes not only to the cell wall, but can also be found in starch bodies in potato plastids (Supplementary Fig. S1b,c). Furthermore, confocal microscopy of A. thaliana transiently transfected with green fluorescent protein (GFP)-fused AtPNP-A confirmed co-localization of PNP in chloroplasts (Supplementary Fig. S1d), consistent with localization of native AtPNP-A both in chloroplasts and mesophyll cell protoplasts (MCPs) isolated from wild type (WT) A. thaliana (Supplementary Fig. S1e). For that reason, cross-linking experiments were performed on MCPs and making use of a C-terminally biotinylated synthetic peptide of AtPNP-A (pAtPNP-A) containing the amino acid sequence of its experimentally determined active region (amino acids 33–66)13,43 (Fig. 1a–b). The pAtPNP-A dependent protoplast swelling response is significantly greater than the response elicited by the scrambled peptide (pScr) (Fig. 1b) and comparable to that elicited by an equimolar amount of the recombinant AtPNP-A (rAtPNP-A) (Fig. 1b). The biologically active pAtPNP-A was subsequently used in binding experiments performed in A. thaliana (Col-0) MCPs in the presence of cross-linking agents enabling identification of weak interactors of AtPNP-A. Cross-linking experiments with biotin in the presence of the cross-linking agent excluded nonspecific interactors, whereas binding of proteins to the biotinylated pAtPNP-A in the absence of the cross-linker allowed identification of interactions lost due to the presence of the cross-linker. Subsequent affinity-based isolation of pAtPNP-A (or biotin) with bound interactors followed by LC–MS/MS identification enabled relative quantification of spectral counts corresponding to peptides matching putative binding partners of pAtPNP-A44.

To further increase confidence in the candidate preys being true positives, a Y2H screen was performed with AtPNP-A (amino acids 26–130), which includes the active region of AtPNP-A corresponding to the amino acid sequence of pAtPNP-A, as a bait (Fig. 1a). The Y2H assay identified 25 candidate AtPNP-A preys, 13 of which showed weak interaction strength (Table 1). Only two proteins were identified as putative interactors of AtPNP-A with both methods: catalase 2 (CAT2; At4g35090; P25819) (Fig. 1c) and rubisco activase (RCA; At2g39730; Q0WLM1) (Table 1). CAT2 was identified by LC–MS/MS only in samples containing pAtPNP-A, while the RCA was also abundant in control samples without the peptide44. PNPs are known to modulate redox signaling20, therefore we tested if flg22, a 22-amino acid peptide derived from bacterial flagellin recognized by the FLS2 receptor57 induced changes in redox signaling. AtPNP enhances responses to flg22 (Supplementary Fig. S2), suggestive of a redox effect so we undertook to test the binding between AtPNP-A and the antioxidant enzyme CAT2.

Table 1 Proteins identified as candidate direct interactors of AtPNP-A in the yeast two-hybrid (Y2H) analysis.

AtPNP-A specifically binds CAT2 in vitro

Protein docking simulations making use of the 3D structure models of AtPNP-A and CAT2 predict interaction of AtPNP-A with the C-terminal portion of CAT2 monomer (Fig. 2a). To assess specificity of any binding of AtPNP-A to CAT2, the proteins were expressed as recombinants, purified (Supplementary Fig. S3a), and in vitro binding of the recombinant proteins measured in both ligand—analyte configurations with surface plasmon resonance (SPR) (Supplementary Fig. S3b,c). The specificity of the interaction between the active region of AtPNP-A and purified CAT2 recombinant (rCAT2) is confirmed in analyses making use of the NTA sensor chip, where the His-tagged rCAT2 is immobilized, while the biologically active pAtPNP-A or the corresponding biologically inactive pScr peptide injected through the flow cells, resulting in a significant accumulation of the biologically active analyte (Fig. 2b) and only negligible binding of pScr (Fig. 2b). This excludes the possibility of a nonspecific interaction between pAtPNP-A and CAT2 that would occur solely on a basis of a nonspecific charge effect residing in the primary structure of the peptide. Binding analyses between pAtPNP-A and bovine CAT and pAtPNP-A performed in both ligand—analyte configurations using the CM5 sensor chip with amine-coupling chemistry-based CAT ligand immobilization also points to a specific interaction. In addition, kinetic analysis reveals strong binding, with the dissociation constant (KD) in the sub-micromolar range (8.6 × 10–8 M; Fig. 2c, Supplementary Table S2). This observation is further confirmed with isothermal titration calorimetry (ITC), revealing significant conformational changes occurring as a result of pAtPNP-A binding to bovine CAT (Supplementary Fig. S4).

Figure 2

AtPNP-A directly interacts with Arabidopsis CAT2 and bovine liver CAT in vitro. (a) Molecular docking of AtPNP-A and CAT2. Surface model depicts predicted docking of AtPNP-A (blue), with its active region (cyan), and CAT2 monomer (tan). The structures of AtPNP-A as well as CAT2 monomer were predicted using the iterative threading assembly refinement (I-TASSER; version 5.1; method53. Protein–protein docking was performed using ClusPro (version 2.0; The models were analyzed and visualized using UCSF Chimera (version 1.10.2; (b) Exemplar sensorgrams depicting referenced binding response of pAtPNP-A or pScr with CAT2 recombinant protein immobilized on the active surface of the NTA sensor chip. Reference surface of the NTA chip was not modified, according to the manufacturer’s instructions, and did not carry the recombinant protein. In both analyses the ligand was immobilized at the same level (app. 4,500 RU), analytes are injected at the same concentration and conditions of runs kept constant. (c) Exemplar sensorgrams depicting referenced binding response in kinetic analysis of binding between pAtPNP-A (3.78 μM and consecutive two-fold dilutions, as in Supplementary Table S2) and bovine liver CAT immobilized on the active surface of the CM5 sensor chip. Reference surface of the NTA chip was not modified, according to the manufacturer’s instructions, and did not carry any protein.

AtPNP-A interaction with CAT2 enhances catalase activity

To test whether the activity of CAT2 is modulated by AtPNP-A in planta, we investigated the basal levels of CAT activity, and the activity of CAT2 in particular, in response to AtPNP-A in WT seedlings and in seedlings with a mutated CAT2 allele. To that end a homozygous mutant line carrying transfer DNA (T-DNA) insertions in CAT2 is used. The cat2-2 is a null mutant (T-DNA insertion located in the third exon; Supplementary Fig. S5a) that exhibits only residual (ca. 20%) extractable CAT enzymatic activity in leaves and less than 50% in roots58,59,60. The lack of CAT2 isoform in the cat2-2 mutant plants was confirmed with native PAGE followed by specific CAT activity staining enabling determination of enzymatic activities of different CAT isoforms (Fig. 3a). Furthermore, exogenous application of nM concentrations of pAtPNP-A, in contrast to the treatment with pScr, results in increased CAT2 activity in WT plants compared to the mutants (Fig. 3a). This again confirms that CAT2 is an isozyme that is enzymatically modulated by AtPNP-A and is consistent with elevated total CAT activity of the protein extracts observed spectrophotometrically after incubation either with pAtPNP-A or the AtPNP-A recombinant (Fig. 3b). Since incubation of the total protein extracted from WT seedlings with pAtPNP-A and rAtPNP-A results in a more rapid H2O2 breakdown (Fig. 3b), we asked whether PNPs are capable of direct modulation of CAT activity. We noted that in vitro enzymatic activity of rCAT2 is significantly enhanced in the presence of rAtPNP-A and pAtPNP-A, but not pScr (Fig. 3c).

Figure 3

AtPNP-A directly interacts with CAT2 to modulate its enzymatic activity in vitro. (a) Zymogram depicting changes in the enzymatic activity of CAT isoforms extracted from wild type (WT) or cat2-2 knockout mutant seedlings in response to 1 nM pAtPNP-A or pScr. Densitometric semi-quantification of bands corresponding to CAT2, normalized to the loading control (dark-coloured band on zymogram) for WT samples treated with 1 nM pAtPNP-A or pScr (mean ± SD, Student’s t-test, n = 3, P < 0.05). Different superscript (a and b) indicates significantly different results. (b) Total CAT activity in protein extracted from WT seedlings assayed with Amplex Red catalase assay kit in the presence of 1 nM pAtPNP-A, rAtPNP-A, or pScr. Different superscript (a, b, and c) indicates significantly different results (mean ± SD, one-way ANOVA, followed by Tukey–Kramer multiple comparison test, n = 3, P < 0.05). (c) Enzymatic activity of rCAT2 in the presence of 100 nM pAtPNP-A, rAtPNP-A, or pScr. Different superscript (a and b) indicates significantly different results from three independent experiments (mean ± SD, one-way ANOVA followed by Tukey–Kramer multiple comparison test, n = 3, P < 0.01).

AtPNP-A interacts with CAT2 in vivo

Physical in planta association of AtPNP-A and CAT2 is confirmed with Bimolecular Fluorescence Complementation (BiFC) assay in tobacco leaf protoplasts (Fig. 4) revealing interaction of the proteins in chloroplasts of protoplasts (Fig. 4a, g). The greatest BiFC / Red fluorescence signal was quantified for interaction between NeYFP fused to CAT2 and CeYFP-tagged AtPNP-A (Fig. 4a) compared with other fusion configurations and controls (Fig. 4g). Lower BiFC/fluorescence signal observed for NeYFP-fused AtPNP-A and CeYFP-tagged CAT2 (Fig. 4b,g) interaction may reflect inhibition of the YFP reconstitution due to spatial restrictions of the three dimensional structure of the protein complex, or steric hindrance caused by the incorporation of the C/NeYFP, or misfolding. The organellar localization of the complementation is not surprising, considering the association of AtPNP-A with plastids and chloroplasts (Supplementary Fig. S1d,e). Notably, while CAT2 is predominantly a peroxisomal protein, its amino acid sequence includes predicted chloroplast transit peptide61 and increasing evidence suggests that it may participate in removal of H2O2 in subcellular compartments other than peroxisomes40,62,63.

Figure 4

AtPNP-A interacts with CAT2 in vivo. (a)(f) BiFC reveals AtPNP-A (indicated as P) and CAT2 (indicated as C) interact in the chloroplasts of tobacco leaf protoplasts. Exemplar merged images of protoplasts isolated from a leaf infiltrated with different combinations of N- or C-terminally tagged CAT2 and AtPNP-A are shown. (g) BiFC/Red fluorescence chloroplast analysis with mean ± SEM in pink. Normalized BiFC fluorescence is significantly higher in chloroplasts from protoplasts expressing NeYFP-CAT2, indicated as NY-C, and CeYFP-AtPNP-A, indicated as CY-P (mean ± SEM, one-way ANOVA followed by Sidak’s post-hoc test, n = 30, ****P < 0.0001).

Next, we asked a question whether CAT2 interaction with AtPNP-A is of physiological significance. To test whether CAT2 activity is affected by AtPNP-A in planta, we examined basal levels of CAT activity, and the activity of CAT2 in particular, using seedlings of a homozygous mutant line carrying T-DNA insertion in AtPNP-A. The atpnp-a mutant is a knockdown line with a T-DNA insertion located in the second intron of AtPNP-A (Supplementary Fig. S5b). Extractable CAT activity is approximately 20% lower in leaves of atpnp-a than in WT plants (Fig. 5a) and the mutant seedlings display decreased activity of the CAT2 isoform (Fig. 5b). The interaction of CAT2 and AtPNP-A is physiologically relevant in germination assays, where atpnp-a phenocopy cat2-2 mutant seeds in impaired growth in the presence of H2O2 (Fig. 5c-d). The impaired germination and growth of seedlings due to their sensitivity to H2O2 is indicative of a disturbance in cellular redox homeostasis in these mutant lines.

Figure 5

The atpnp-a mutant plants phenocopy CAT2-deficient plants in their ability to cope with H2O2 stress. (a) Total CAT enzymatic activities in equal amounts of protein extracted from leaves of 4 week-old WT and atpnp-a mutant seedlings. The graph shows data from three independent experiments (mean ± SD, Student’s t-test, n = 3, * P = 0.0409). (b) Zymogram of CAT isoform activities in protein extracts from atpnp-a or WT seedlings separated in 8% native PAGE and stained specifically for CAT activity. Coomassie brilliant blue (CBB) shows equal loading. Full-length gels are presented in Supplementary Fig. S5a. (c) Germination of WT, cat2-2, and atpnp-a seeds 14 days after sowing on MS agar supplemented with 3 mM H2O2. (d) Quantification of germination (as shown in section (c)) by the presence of green cotyledons. The graph shows data from three independent experiments (mean ± SD, one-way ANOVA followed by Tukey–Kramer multiple comparison test, n = 300, ** P < 0.01, *** P < 0.001).


PNPs are implicated in plant responses to biotic32,64 and abiotic stresses13,65, and partially may do so by modulating cellular levels of ROS20. Pre-treatment of leaf discs with PNP prior to treatment with flg22 enhances oxidative burst (Supplementary Fig. S2), indicating that PNPs temper host resistance against pathogens such as Pst DC300016,17 at least in part, by modulating redox signaling. Whereas it is plausible that PNPs affect redox signaling in multiple ways in different cell types under specific conditions, here we reveal that one of these mechanisms involves a direct interaction of AtPNP-A with CAT2 (Table 1, Fig. 2a,b). In vivo interaction between these proteins is observed by co-localization in chloroplasts (Fig. 4), pointing at compartmentalization of cellular processes modulated by AtPNP-A that involve regulation of ROS homeostasis, and is consistent with cellular localization of PNPs (Supplementary Fig. S1d,e). Binding of AtPNP-A to CAT2 enhances its enzymatic activity (Fig. 3). The atpnp-a knockdown phenocopies CAT2-deficient mutant seeds with compromised sensitivity to H2O2 (Fig. 5c-d). This may result from decreased extractable total CAT activity (Fig. 5a), and CAT2 activity in particular (Fig. 5b), in the atpnp-a knockdown line compared to the WT plants. The specific AtPNP-A and CAT2 interaction in vitro (Fig. 2a, Supplementary Fig. S3b,c) and in vivo (Fig. 4) lend support to the idea that the differences in CAT2 activity result from direct interaction of the enzyme with AtPNP-A. Taken together, these results indicate that AtPNP-A may affect plant responses to abiotic stresses, including response to oxidative stress during germination (Fig. 5c,d), by modulating redox homeostasis via direct interaction with CAT2.

Several lines of evidence indicate AtPNP-A has a protective role in the response to infection with P. syringae16,17. Our results suggest that this may be a consequence of an augmented oxidative burst generated upon perception of elicitors, such as flg22, in the presence of PNPs (Supplementary Fig. S2), since exogenous H2O2 enhances plant pathogen resistance66. This is also consistent with an AtPNP-A function in hyper-activation of signaling mediated by plasma membrane-localized pattern recognition receptors (PRRs) that perceive pathogen-derived molecules4, and with its perception by receptors16,21. Like animal PRRs, plant PRRs activate innate immune responses to fend off pathogens4. The enhanced flg22-induced oxidative burst observed upon pre-incubation of leaf discs with AtPNP-A not only supports the protective function of PNPs against pathogens by enhancing plant innate immunity17, but also indicates that it does so by modulating redox signaling. Nevertheless, the process by which AtPNP-A pre-treatment of leaf discs enhances flg22-oxidative burst is far from resolved. In contrast to flg22, pAtPNP-A alone does not activate mitogen-activated protein kinase (MAPK) pathway (data not shown), but PNP could for instance affect accumulation of some of its components, either at the transcript or protein level20, priming the plant for subsequent pathogen attack and thereby enabling a quicker response by generating a more pronounced radical burst e.g. via the modulation of the plasma membrane NADPH oxidase by AtPNP-A. However, neither NADPH oxidases nor peroxidases, enzymes responsible for ROS generation upon pathogen attack67, have been identified in our protein–protein interaction studies, although animal analogues of PNP have been shown or speculated to activate NADPH oxidase68,69. Instead, we identified CAT2 (Fig. 1c, Table 1), an enzyme crucial for scavenging excess H2O2 produced during pathogenesis70, as a binding partner of AtPNP-A.

Importantly, plant pathogens can activate CATs in response to oxidative bursts, either to strengthen pathogen cell walls or to compromise ROS-mediated host defense, and in many cases CATs have been identified as virulence factors, e.g. in P. syringae DC300071. In Xanthomonas axonopodis pv. citri, a PNP-like protein (XacPNP) is induced in the pathogen during the plant-based stages of the life cycle64. PNP-like molecules are expressed by several plant pathogens64,72 and are considered a double-edged sword for both partners in the plant-pathogen arms race.

A PNP—CAT interaction in plant responses to infecting pathogens is not surprising. CATs commonly function to down-regulate ROS during ABA-induced stomatal closure, and ABA-induced stomatal closure can be inhibited by removal of H2O2 via CAT273,74. AtPNP-A has been shown to significantly delay and reduce the extent of ABA-induced stomatal closure, while ABA had no effect on either AtPNP-A-dependent guard cell volume increases or AtPNP-A-dependent cGMP increases13. Therefore, it is tempting to speculate that AtPNP-A modulates the effect of ABA on stomatal aperture through the interaction with CAT2 (and/or other CATs), enhancing its enzymatic activity in vivo as part of the in planta function.

It is also conceivable that modulation of CAT2 activity by AtPNP-A may not only have implications for plant biotic stress tolerance but may also result in changes in responses to abiotic stress. Activity of CAT2 is required for decomposition of photorespiration-derived H2O2 preventing redox perturbation under ambient growth conditions59 while CAT2 deficiency causes a defective photorespiration phenotype including suppressed growth58,59 and accumulation of H2O2 in leaves75. During senescence suppression of CAT2 function, mediated by G-BOX BINDING FACTOR1 (GBF1)76, decreases CAT2 activity and increases ROS accumulation77. Interestingly, AtPNP-A is a senescence-enhanced gene65 and atpnp-a knock-down seedlings display premature senescence phenotype (data not shown), thereby phenocopying cat2 mutant plants59. Increases in CAT2 transcript were observed upon dehydration78 as well as exogenous application of SA, H2O2, and a superoxide-generating herbicide methyl viologen56, with the latter causing extensive chlorosis in AtPNP-A-deficient seedlings17.

On a more general level, it is not surprising that many hormone receptors have enzymatic functions, since ligand binding needs to directly translate into a molecular response. Activation of kinase receptors by ligand binding is followed by consequent specific phosphorylation events. Natriuretic peptide receptors A and B contain cytosolic guanylate cyclase domains activated upon ligand binding79. This ligand-dependent activation of the cyclase causes the generation of cGMP, which in turn acts as second messenger for the multiple downstream responses. Given the perhaps surprising similarities between the vertebrate and the plant NP receptors, our result begs the question if possibly vertebrate NPs can also modulate vertebrate catalases. Realization that AtPNP-A is capable of undergoing specific in vitro interaction with monofunctional CAT proteins derived from different sources, including bovine (Fig. 2c, Supplementary Fig. S4), prompts speculation that not only CAT2 interacts with PNPs, but it is possible that the interaction between NPs and CAT proteins is not exclusive to the plant kingdom and may be prevalent in other systems.

In summary, we present evidence for a specific and direct interaction between AtPNP-A and CAT2 and propose that the AtPNP-A modulated CAT2 activity ensures the maintenance of an optimized redox state during plant stress responses. Since CAT2 is not limited to protecting cells from H2O2, but also modulates its level to maintain optimal redox states of antioxidants, including ascorbate or glutathione, our study on PNP-dependent modulation of CAT2 activity may inform further research into H2O2 signaling and whole cell reduction–oxidation homeostasis.

Data availability

Complete proteomics data set has been published in44.


  1. 1.

    Hirayama, T. & Shinozaki, K. Research on plant abiotic stress responses in the post-genome era: Past, present and future. Plant J. 61, 1041–1052 (2010).

    CAS  Article  Google Scholar 

  2. 2.

    Reddy, A. S. N., Ali, G. S., Celesnik, H. & Day, I. S. Coping with stresses: roles of calcium- and calcium/calmodulin-regulated gene expression. Plant Cell 23, 2010–2032 (2011).

    CAS  Article  Google Scholar 

  3. 3.

    Lemtiri-Chlieh, F., Thomas, L., Marondedze, C., Irving, H. & Gehring, C. Cyclic nucleotides and nucleotide cyclases in plant stress responses. In Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives (ed. Shanker, A.) 137–182 (InTech, 2012).

  4. 4.

    Couto, D. & Zipfel, C. Regulation of pattern recognition receptor signalling in plants. Nat. Rev. Immunol. 16, 537–552 (2016).

    CAS  Article  Google Scholar 

  5. 5.

    Klingler, J. P., Batelli, G. & Zhu, J. ABA receptors: The START of a new paradigm in phytohormone signalling. J. Exp. Bot. 61, 3199–3210 (2010).

    CAS  Article  Google Scholar 

  6. 6.

    Zhang, X. L. et al. Structural basis and functions of abscisic acid receptors PYLs. Front. Plant Sci. 6, 88. (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. 7.

    Ooi, A., Lemtiri-Chlieh, F., Wong, A. & Gehring, C. Direct modulation of the guard cell outward-rectifying potassium channel (GORK) by abscisic acid. Mol. Plant 10, 1469–1472 (2017).

    CAS  Article  Google Scholar 

  8. 8.

    Gehring, C. & Irving, H. R. In Plant Signaling Peptides (eds Irving, H. R. & Gehring, C.) (Springer, New York, 2012).

    Google Scholar 

  9. 9.

    Hirakawa, Y., Torii, K. U. & Uchida, N. Mechanisms and strategies shaping plant peptide hormones. Plant Cell Physiol. 58, 1313–1318 (2017).

    CAS  Article  Google Scholar 

  10. 10.

    Ruzvidzo, O., Donaldson, L., Valentine, A. & Gehring, C. The Arabidopsis thaliana natriuretic peptide AtPNP-A is a systemic regulator of leaf dark respiration and signals via the phloem. J. Plant Physiol. 168, 1710–1714 (2011).

    CAS  Article  Google Scholar 

  11. 11.

    Gehring, C. A. Natriuretic peptides—A new class of plant hormone?. Ann. Bot. 83, 329–334 (1999).

    CAS  Article  Google Scholar 

  12. 12.

    Gehring, C. & Irving, H. Plant natriuretic peptides: Systemic regulators of plant homeostasis and defense that can affect cardiomyoblasts. J. Investig. Med. 61, 823–826 (2013).

    CAS  Article  Google Scholar 

  13. 13.

    Wang, Y. H., Gehring, C., Cahill, D. M. & Irving, H. R. Plant natriuretic peptide active site determination and effects on cGMP and cell volume regulation. Funct. Plant Biol. 34, 645–653 (2007).

    CAS  Article  Google Scholar 

  14. 14.

    Maryani, M. M., Bradley, G., Cahill, D. M. & Gehring, C. A. Natriuretic peptides and immunoreactants modify osmoticum-dependent volume changes in Solanum tuberosum L. mesophyll cell protoplasts. Plant Sci. 161, 443–452 (2001).

    CAS  Article  Google Scholar 

  15. 15.

    Ludidi, N. et al. A recombinant plant natriuretic peptide causes rapid and spatially differentiated K+, Na+ and H+ flux changes in Arabidopsis thaliana roots. Plant Cell Physiol. 45, 1093–1098 (2004).

    CAS  Article  Google Scholar 

  16. 16.

    Lee, K. P. et al. Plant natriurteic peptide A and its putative receptor PNP-R2 antagonize salicylic acid-mediated signaling and cell death. Plant Cell 32, 2237–2250 (2020).

    CAS  Article  Google Scholar 

  17. 17.

    Ficarra, F. A., Grandellis, C., Garavaglia, B. S., Gottig, N. & Ottado, J. Bacterial and plant natriuretic peptides improve plant defence responses against pathogens. Mol. Plant Pathol. 19, 801–811 (2018).

    CAS  Article  Google Scholar 

  18. 18.

    Zois, N. E. et al. Natriuretic peptides in cardiometabolic regulation and disease. Nat. Rev. Cardiol. 11, 403–412 (2014).

    CAS  Article  Google Scholar 

  19. 19.

    Pharmawati, M., Maryani, M. M., Nikolakopoulos, T., Gehring, C. A. & Irving, H. R. Cyclic GMP modulates stomatal opening induced by natriuretic peptides and immunoreactive analogues. Plant Physiol. Biochem. 39, 385–394 (2001).

    CAS  Article  Google Scholar 

  20. 20.

    Turek, I., Marondedze, C., Wheeler, J., Gehring, C. & Irving, H. Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress. Front. Plant Sci. 5, 661. (2014).

    Article  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Turek, I. & Gehring, C. The plant natriuretic peptide receptor is a guanylyl cyclase and enables cGMP-dependent signaling. Plant Mol. Biol. 91, 275–286 (2016).

    CAS  Article  Google Scholar 

  22. 22.

    Barba-Espín, G. et al. Understanding the role of H2O2 during pea seed germination: A combined proteomic and hormone profiling approach. Plant Cell Environ. 34, 1907–1919 (2011).

    Article  CAS  Google Scholar 

  23. 23.

    Wojtyla, Ł, Lechowska, K., Kubala, S. & Garnczarska, M. Different modes of hydrogen peroxide action during seed germination. Front. Plant Sci. 7, 66. (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  24. 24.

    Wang, P. & Song, C. P. Guard-cell signalling for hydrogen peroxide and abscisic acid. New Phytol. 178, 703–718 (2008).

    ADS  CAS  Article  Google Scholar 

  25. 25.

    Shi, K. et al. Guard cell hydrogen peroxide and nitric oxide mediate elevated CO2-induced stomatal movement in tomato. New Phytol. 208, 342–353 (2015).

    CAS  Article  Google Scholar 

  26. 26.

    Ge, X. M. et al. Heterotrimeric G protein mediates ethylene-induced stomatal closure via hydrogen peroxide synthesis in Arabidopsis. Plant J. 82, 138–150 (2015).

    CAS  Article  Google Scholar 

  27. 27.

    Arnaud, D. & Hwang, I. A sophisticated network of signaling pathways regulates stomatal defenses to bacterial pathogens. Mol. Plant 8, 566–581 (2015).

    CAS  Article  Google Scholar 

  28. 28.

    Houot, V. et al. Hydrogen peroxide induces programmed cell death features in cultured tobacco BY-2 cells, in a dose-dependent manner. J. Exp. Bot. 52, 1721–1730 (2001).

    CAS  PubMed  Google Scholar 

  29. 29.

    Gechev, T. S. & Hille, J. Hydrogen peroxide as a signal controlling plant programmed cell death. J. Cell Biol. 168, 17–20 (2005).

    CAS  Article  Google Scholar 

  30. 30.

    Mittler, R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7, 405–410 (2002).

    CAS  Article  Google Scholar 

  31. 31.

    Morse, M., Pironcheva, G. & Gehring, C. AtPNP-A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation. FEBS Lett. 556, 99–103 (2004).

    CAS  Article  Google Scholar 

  32. 32.

    Breitenbach, H. H. et al. Contrasting roles of the apoplastic aspartyl protease apoplastic, enhanced disease susceptibility-dependent1 and legume lectin-like protein1 in Arabidopsis systemic acquired resistance. Plant Physiol. 165, 791–809 (2014).

    CAS  Article  Google Scholar 

  33. 33.

    de Azevedo Neto, A. D., Prisco, J. T., Enéas-Filho, J., Rolim Medeiros, J. V. & Gomes-Filho, E. Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants. J. Plant Physiol. 162, 1114–1122 (2005).

    Article  CAS  Google Scholar 

  34. 34.

    Gondim, F. A., Gomes-Filho, E., Costa, J. H., Mendes Alencar, N. L. & Prisco, J. T. Catalase plays a key role in salt stress acclimation induced by hydrogen peroxide pretreatment in maize. Plant Physiol. Biochem. 56, 62–71 (2012).

    CAS  Article  Google Scholar 

  35. 35.

    Cruz de Carvalho, M. H. Drought stress and reactive oxygen species. Plant Signal. Behav. 3, 156–165 (2008).

    Article  Google Scholar 

  36. 36.

    Khan, A. et al. Attenuation of drought stress in Brassica seedlings with exogenous application of Ca2+ and H2O2. Plants 6, 20. (2017).

    CAS  Article  Google Scholar 

  37. 37.

    Hossain, M. A. et al. Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Front. Plant Sci. 6, 420. (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  38. 38.

    Bolwell, G. P. et al. The apoplastic oxidative burst in response to biotic stress in plants: A three-component system. J. Exp. Bot. 53, 1367–1376 (2002).

    CAS  PubMed  PubMed Central  Google Scholar 

  39. 39.

    Frugoli, J. et al. Catalase is encoded by a multigene family in Arabidopsis thaliana. Plant Physiol. 112, 327–336 (1996).

    CAS  Article  Google Scholar 

  40. 40.

    Willekens, H. et al. Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. EMBO J. 16, 4806–4816 (1997).

    CAS  Article  Google Scholar 

  41. 41.

    Alonso, J. M. et al. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301, 653–657 (2003).

    ADS  Article  Google Scholar 

  42. 42.

    Singh, M., Taylor, P., Knox, B. & R. , Special preparation methods for immunocytochemistry of plant cells. In Immunocytochemistry. A Practical Approach (ed. Beesley, J.) 77–102 (IRL Press, Drosophila, 1993).

    Google Scholar 

  43. 43.

    Pharmawati, M., Billington, T. & Gehring, C. A. Stomatal guard cell responses to kinetin and natriuretic peptides are cGMP-dependent. Cell. Mol. Life Sci. 54, 272–276 (1998).

    CAS  Article  Google Scholar 

  44. 44.

    Turek, I., Irving, H. & Gehring, C. Dataset on interactors of the Arabidopsis thaliana plant natriuretic peptide (AtPNP-A) determined by mass spectrometry. Data Br. 30, 105606. (2020).

    Article  Google Scholar 

  45. 45.

    Gietz, R. D. & Woods, R. A. Review genetic transformation of yeast. Biotechniques 831, 816–831 (2001).

    Article  Google Scholar 

  46. 46.

    Wang, Y. H., Gehring, C. & Irving, H. R. Plant natriuretic peptides are apoplastic and paracrine stress response molecules. Plant Cell Physiol. 52, 837–850 (2011).

    CAS  Article  Google Scholar 

  47. 47.

    Triboush, S., Danilenko, N. & Davydenko, O. A method for isolation of chloroplast DNA and mitochondrial DNA from sunflower. Plant Mol. Biol. Report. 16, 183–189 (1998).

    CAS  Article  Google Scholar 

  48. 48.

    Towbin, H., Staehelint, T. & Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc. Natl. Acad. Sci. USA 76, 4350–4354 (1979).

    ADS  CAS  Article  Google Scholar 

  49. 49.

    Wang, Y. H., Ahmar, H. & Irving, H. R. Induction of apoptosis by plant natriuretic peptides in rat cardiomyoblasts. Peptides 31, 1213–1218 (2010).

    CAS  Article  Google Scholar 

  50. 50.

    Wheeler, J. I. et al. The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling. Plant J. 91, 590–600 (2017).

    CAS  Article  Google Scholar 

  51. 51.

    Yoo, S.-D., Cho, Y.-H. & Sheen, J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat. Protoc. 2, 1565–1572 (2007).

    CAS  Article  Google Scholar 

  52. 52.

    Kodama, Y. Time gating of chloroplast autofluorescence allows clearer fluorescence imaging in planta. PLoS ONE 11, 3 (2016).

    Article  CAS  Google Scholar 

  53. 53.

    Zhang, Y. I-TASSER server for protein 3D structure prediction. BMC Bioinform. 9, 40. (2008).

    CAS  Article  Google Scholar 

  54. 54.

    Comeau, S. R., Gatchell, D. W., Vajda, S. & Camacho, C. J. ClusPro: An automated docking and discrimination method for the prediction of protein complexes. Bioinformatics 20, 45–50 (2004).

    CAS  Article  Google Scholar 

  55. 55.

    Pettersen, E. F. et al. UCSF Chimera—A visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605–1612 (2004).

    CAS  Article  Google Scholar 

  56. 56.

    Du, Y.-Y., Wang, P.-C., Chen, J. & Song, C.-P. Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana. J. Integr. Plant Biol. 50, 1318–1326 (2008).

    CAS  Article  Google Scholar 

  57. 57.

    Chinchilla, D. et al. The Arabidopsis receptor kinase FLS2 binds flg22 and determines the specificity of flagellin perception. Plant Cell 18, 465–476 (2006).

    CAS  Article  Google Scholar 

  58. 58.

    Bueso, E. et al. The lithium tolerance of the Arabidopsis cat2 mutant reveals a cross-talk between oxidative stress and ethylene. Plant J. 52, 1052–1065 (2007).

    ADS  CAS  Article  Google Scholar 

  59. 59.

    Queval, G. et al. Conditional oxidative stress responses in the Arabidopsis photorespiratory mutant cat2 demonstrate that redox state is a key modulator of daylength-dependent gene expression, and define photoperiod as a crucial factor in the regulation of H2O2-induced cel. Plant J. 52, 640–657 (2007).

    CAS  Article  Google Scholar 

  60. 60.

    Yang, Z., Mhamdi, A. & Noctor, G. Analysis of catalase mutants underscores the essential role of catalase2 for plant growth and day length-dependent oxidative signalling. Plant. Cell Environ. 42, 688–700 (2018).

    Article  CAS  Google Scholar 

  61. 61.

    Emanuelsson, O., Nielsen, H. & von Heijne, G. ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci. 8, 978–984 (2009).

    Article  Google Scholar 

  62. 62.

    Mhamdi, A., Noctor, G. & Baker, A. Plant catalases: Peroxisomal redox guardians. Arch. Biochem. Biophys. 525, 181–194 (2012).

    CAS  Article  Google Scholar 

  63. 63.

    Su, T. et al. The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome-derived signaling in plant development. J. Integr. Plant Biol. 60, 591–607 (2018).

    CAS  Article  Google Scholar 

  64. 64.

    Gottig, N. et al. Xanthomonas axonopodis pv. citri uses a plant natriuretic peptide-like protein to modify host homeostasis. Proc. Natl. Acad. Sci. USA 105, 18631–18636 (2008).

    ADS  CAS  Article  Google Scholar 

  65. 65.

    Meier, S. et al. Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides. BMC Plant Biol. 8, 24. (2008).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  66. 66.

    Hong, J. K. et al. Hydrogen peroxide-and nitric oxide-mediated disease control of bacterial wilt in tomato plants. Plant Pathol. J. 29, 386–396 (2013).

    CAS  Article  Google Scholar 

  67. 67.

    Mammarella, N. et al. The apoplastic oxidative burst peroxidase in Arabidopsis is a major component of pattern-triggered immunity. Plant Cell 24, 275–287 (2012).

    Article  CAS  Google Scholar 

  68. 68.

    Baldini, P. M. et al. Atrial natriuretic peptide induces cell death in human hepatoblastoma (HepG2) through the involvement of NADPH oxidase. Cell Death Differ. 11, S210–S212. (2004).

    CAS  Article  PubMed  Google Scholar 

  69. 69.

    Pernomian, L. et al. C-type natriuretic peptide induces anti-contractile effect dependent on nitric oxide, oxidative stress, and NPR-B activation in sepsis. Front. Physiol. 7, 226. (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  70. 70.

    Simon, C. et al. The differential spatial distribution of secondary metabolites in Arabidopsis leaves reacting hypersensitively to Pseudomonas syringae pv. tomato is dependent on the oxidative burst. J. Exp. Bot. 61, 3355–3370 (2010).

    CAS  Article  Google Scholar 

  71. 71.

    Guo, M., Block, A., Bryan, C. D., Becker, D. F. & Alfano, J. R. Pseudomonas syringae catalases are collectively required for plant pathogenesis. J. Bacteriol. 194, 5054–5064 (2012).

    CAS  Article  Google Scholar 

  72. 72.

    de Jonge, R. et al. Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing. Proc. Natl. Acad. Sci. USA 109, 5110–5115 (2012).

    ADS  Article  Google Scholar 

  73. 73.

    Jannat, R. et al. The roles of catalase2 in abscisic acid signaling in Arabidopsis guard cells. Biosci. Biotechnol. Biochem. 75, 2034–2036 (2011).

    CAS  Article  Google Scholar 

  74. 74.

    Jannat, R. et al. Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells. J. Plant Physiol. 168, 1919–1926 (2011).

    CAS  Article  Google Scholar 

  75. 75.

    Hu, Y.-Q. et al. Functional comparison of catalase genes in the elimination of photorespiratory H2O2 using promoter- and 3′-untranslated region exchange experiments in the Arabidopsis cat2 photorespiratory mutant. Plant. Cell Environ. 33, 1656–1670 (2010).

    CAS  Article  Google Scholar 

  76. 76.

    Giri, M. K. et al. GBF1 differentially regulates CAT2 and PAD4 transcription to promote pathogen defense in Arabidopsis thaliana. Plant J. 91, 802–815 (2017).

    CAS  Article  Google Scholar 

  77. 77.

    Zimmermann, P., Heinlein, C., Orendi, G. & Zentgraf, U. Senescence-specific regulation of catalases in Arabidopsis thaliana (L.) Heynh. Plant Cell Environ. 29, 1049–1060 (2006).

    CAS  Article  Google Scholar 

  78. 78.

    Fichman, Y. et al. Selenoprotein O is a chloroplast protein involved in ROS scavenging and its absence increases dehydration tolerance in Arabidopsis thaliana. Plant Sci. 270, 278–291 (2018).

    CAS  Article  Google Scholar 

  79. 79.

    Misono, K. S. et al. Structure, signaling mechanism and regulation of the natriuretic peptide receptor guanylate cyclase. FEBS J. 278, 1818–1829 (2011).

    CAS  Article  Google Scholar 

Download references


Funding was provided by Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology. Ilona Turek was supported by a King Abdullah University of Science and Technology doctoral scholarship. Part of this work was supported by the Australian Research Council [Discovery Project funding scheme (DP0557561, DP0878194)], Australian Postgraduate Award to YHW, and also by La Trobe University. We thank the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion mutants and Dr Peter Lock (La Trobe University LIMS BioImaging Facility) for assistance with imaging.

Author information




C.G., H.R.I. and I.T. conceived the project. I.T., J.W., S.B., J.S., Y.H.W., P.T., C.G., and H.R.I designed and performed the experiments. I.T., C.G. and H.R.I. wrote the manuscript. All authors reviewed the manuscript.

Corresponding authors

Correspondence to Ilona Turek or Helen Irving.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Turek, I., Wheeler, J., Bartels, S. et al. A natriuretic peptide from Arabidopsis thaliana (AtPNP-A) can modulate catalase 2 activity. Sci Rep 10, 19632 (2020).

Download citation


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links