Sufficient tissue oxygenation is required for regular brain function; thus oxygen supply must be tightly regulated to avoid hypoxia and irreversible cell damage. If hypoxia occurs the transcription factor complex hypoxia-inducible factor (HIF) will accumulate and coordinate adaptation of cells to hypoxia. However, even under atmospheric O2 conditions stabilized HIF-2α protein was found in brains of adult mice. Mice with a neuro-specific knockout of Hif-2α showed a reduction of pyramidal neurons in the retrosplenial cortex (RSC), a brain region responsible for a range of cognitive functions, including memory and navigation. Accordingly, behavioral studies showed disturbed cognitive abilities in these mice. In search of the underlying mechanisms for the specific loss of pyramidal cells in the RSC, we found deficits in migration in neural stem cells from Hif-2α knockout mice due to altered expression patterns of genes highly associated with neuronal migration and positioning.
Oxygen is essential for most life on earth and affects various life activities including growth and development. Although oxygen is vital to maintain normal function of all organs, oxygen levels in the tissue are always substantially lower than the pO2 in the air we breathe1. Especially in the brain, which critically depends on oxygen supply2, oxygen levels in almost all regions are rather low in the adult (pO2 of 11.4—53.2 mmHg) or even lower in the fetal brain3 (0.076—7.6 mmHg). Thus, oxygen supply must precisely match the local demand to prevent critical hypoxia with irreversible brain damage2.
Hypoxia initiates a wide range of cellular responses to maintain oxygen supply, including altered gene expression to optimize supply and adapt metabolism. 89% of hypoxia-inducible genes appear to have a common mode of regulation that involves activation of hypoxia-inducible factor (HIF), an oxygen-responsive member of the helix-loop-helix PAS (PER-ARNT-SIM) family4. HIF is a heterodimer composed of an oxygen-sensitive α-subunit (HIFα) and a constitutive β-subunit (HIF-1β, also known as ARNT). Three HIFα subunits have been reported: while HIF-1α or HIF-2α (EPAS1) dimerizes with HIF-1β to drive hypoxia-induced target gene expression, the function of HIF-3α is less obvious5. In normoxic conditions, prolyl hydroxylases (PHD-1—PHD-3) and von Hippel-Lindau protein (VHL) target HIF-1/2α for proteasomal degradation6,7. Under hypoxia, oxygen-dependent PHD activity ceases and HIFαs accumulate due to reduced degradation.
Hif-1α, Hif-2α and Hif-1β are expressed in the developing brain, and modulate gene activity in response to low oxygen in the brain in vivo8. Neural cell-specific Hif-1α deficient mice exhibit a hydrocephalus accompanied by a reduction of neuronal cells and an impairment of spatial memory, indicating that HIF is crucial for brain development9. However, the role of HIF-2α during neural development is still not well understood. Duan et al. showed that a loss of HIF-2α in astrocytes leads to disturbed astrogenesis in the murine retina10. In zebrafish, HIF-2α protects neural progenitor cells and neural differentiation processes by up-regulating the survival orthologues Birc5a and Birg5b during embryogenesis11. A recent study investigating the role of astrocytic HIF-1α and HIF-2α in synaptic plasticity found that loss of HIF-2α could affect cognitive performance in mice12.
The aim of our study was to investigate the role of HIF-2 in the murine brain. Since we found HIF-2α protein already stabilized in the normoxic brain of wild type animals, we bred a neural specific Hif-2α knockout mouse to investigate the role of HIF-2 in normal brain function with a focus on development.
HIF-2α is stabilized in the normoxic mouse brain
To determine the role of HIF in the brain, we quantified Hif-1α and Hif-2α expression in the adult brain (P70) using in situ hybridization (Fig. 1A). Hif-1α mRNA was ubiquitously expressed and equally distributed in all brain cells. Hif-2α was strongly expressed in endothelial cells of blood vessels, but was also present in all other cell types of the brain, including cells in adult stem cell niches, like the subventricular and subgranular zone. Moreover, expression of Hif-2α was significantly higher than Hif-1α (Fig. 1B and C). Because HIF-α is acutely regulated on the protein level by hypoxic post-translational stabilization, we determined protein levels using immunohistochemistry (Fig. 1D). To our surprise, HIF-2α protein was found throughout the brain in conventionally housed animals kept under atmospheric O2 conditions. In contrast, HIF-1α protein was not detected in the normoxic brain and was only stabilized after hypoxic treatment (Fig. 1D and E). Thus, we found HIF-2α constantly expressed and stabilized in the normoxic adult mouse brain.
Hif-2α knockout in the brain leads to a significant loss of pyramidal cells in the retrosplenial cortex
As HIF-2α was constantly present in the brain, it likely has an important role in normal brain function. To investigate the impact during brain development, we created a conditional brain specific Hif-2α knockout mouse by crossing mice with double-floxed Hif-2α (exon 2 flanked by loxP sites) with mice heterozygous for Cre recombinase under control of the Nestin promoter. Thus, Hif-2α+f/+f x Nes-Cre+/- mice have a dysfunctional HIF-2α protein lacking the DNA-binding domain in neural progenitor and all descending cells (Suppl. Figure s1). Hif-2α+f/+f x Nes-Cre+/- animals, in the following termed Hif-2α-/-, had a normal life span and fertility, and showed no obvious neurological deficits. Brain weight and morphometrics13 did not differ from wild type (Hif-2α+f/+f) littermates (Fig. 2A). In H&E staining of sagittal sections the maximal cortex width was slightly reduced in Hif-2α-/- animals compared to wild type mice (Fig. 2B and C). Additionally, counts on Nissl stained sections revealed a reduced number of pyramidal cells in the retrosplenial cortex (RSC) by more than 50% (Fig. 2D). In contrast, pyramidal cells in the prefrontal cortex (PFC) and the hippocampus were not affected (data not shown). The loss of pyramidal cells was confirmed by immunohistochemistry, mRNA, and protein analysis for neurogranin (Fig. 2B and 2E, Suppl. Figure s2). Moreover, we found a reduction in myelin basic protein (Mbp) and synapsin 1 (Syn1) expression in the cortex of Hif-2α-/- mice (Fig. 2B and 2E). We also looked for morphological changes in other cell types that express Nestin during their development, like pericytes and endothelial cells, but did not find any substantial differences.
Hif-2α knockout mice have impaired learning, memory, and fear induction
The RSC is key in a core network of brain regions important for several cognitive functions. Moreover, the RSC is regularly compromised in common neurological disorders that impair memory14. We performed different tests to investigate, if the observed alterations in the RSC lead to disturbances in motor abilities (Rotarod), behavior (light–dark box), and learning and memory (novel object recognition test, NOR). In the Rotarod test, Hif-2α-/- mice showed slightly enhanced motor skills (Fig. 2F). The light–dark box indicated a reduced fear-associated behavior in the knockout animals (Fig. 2G), which spent nearly twice as much time in the lighted department, which is typically avoided. However, results in both tests were not statistically significant due to high individual differences in both groups. Interestingly, the NOR test showed diminished abilities in learning and memory after loss of HIF-2α (Fig. 2H). The differentiation index represents the capability to distinguish between two different objects, respectively the capability to remember the old object and identify the new one, which relates to learning, whereas the recognition index characterizes memory function. Both indices revealed significantly poorer competences of Hif-2α-/- mice compared to wild type animals. In conclusion our results demonstrate that loss of HIF-2α during brain development leads to loss of predominantly pyramidal cells in the RSC, accompanied by severe impairments in learning and memory.
Loss of HIF-2α in NSC affects various parameters during neural development
For mechanistic studies, we employed the neurosphere system15. Neural stem cells were isolated from new born (P0) wild type and knockout pups and cultivated as neurospheres under atmospheric O2 concentrations. For experiments, proliferating or differentiating neurospheres were incubated under normoxic (20.9% O2), hypoxic (1% O2) or severely hypoxic (0.2% O2) conditions. First, we studied proliferation in wild type and knockout spheres. Size of the spheres, which is linearly correlated with cell numbers15, was assessed after 1, 4, and 7 days. Over time, spheres grew under all three oxygen conditions, although proliferation was highest under normoxic and lowest under severely hypoxic conditions (Fig. 3A). No differences between wild type and knockout spheres were found. Next, to investigate migration, neurospheres were plated on a protein matrix. After mitogen withdrawal, neural cells started to migrate radially out of the spheres with increasing distances over time. Migration was quantified after 24, 48, and 72 h. In wild type cells, reduced O2 concentrations caused shorter migration (Fig. 3B). Interestingly, Hif-2α-/- cell migration was significantly lower under normoxia compared to wild type controls but this difference was lost with increasing hypoxia. To analyses, if the reduction in migration was caused by cell death, we performed a cell viability assay. Oxygen concentration had a major impact on cell viability and induced higher cell death rates under hypoxia. At all oxygen levels, the number of dead cells in the Hif-2α-/- neurospheres was lower compared to wild type cells indicating a crucial effect of HIF-2α concerning cell death (Fig. 3C). As the loss of HIF-2α led to reduced numbers of pyramidal cells in the RSC and a reduction in MBP, we analyzed differentiation into neurons and oligodendrocytes in vitro. First, we checked for changes in mRNA expression of β(III)-Tubulin during differentiation. We found no significant differences between wild type and knockout spheres or different oxygen concentration (Fig. 3D). However, immunocytochemical staining against β(III)-Tubulin showed a significant reduction in immunoreactive cells in HIF-2α deficient neurospheres under normoxia, but not under hypoxia or severe hypoxia (Fig. 3G and 3H). Interestingly, no morphological differences were seen. To analyze oligodendrocyte development, we looked for Cnp expression for OPCs and Mbp expression as a marker for mature oligodendrocytes and found a strong inhibition of both genes under hypoxia (Fig. 3E and F). A significant difference between knockout and wild type cells was only observed under severely hypoxic conditions, where almost no Cnp and Mbp was detectable in the knockout spheres. Yet, immunocytochemical staining against the O4 epitope revealed no differences in oligodendrocyte numbers and morphologies between wild type and knockout spheres (Fig. 3I and J), although prolonged incubation under hypoxic or severely hypoxic conditions reduced the overall number of O4+ cells in both genotypes.
Deficiency in HIF-2α affects synaptogenesis
Beside the reduced Neurogranin mRNA expression in the cortices of Hif-2α-/- animals, we also detected a reduction in Syn1 expression. Therefore, we measured mRNA levels of the synapse associated proteins Syn1 and SAP90/PSD-95-associated protein 4 (Dlgap4) in neurospheres under different oxygen concentrations (Fig. 4A). Spheres derived from Hif-2α-/- mice showed a strong reduction in the expression of Syn1 and Dlgap4 under normoxia and hypoxia. Under severe hypoxia, hardly any of these mRNAs were detectable in both groups. Based on these in vivo and in vitro findings of reduced synaptic mRNA expression, we analyzed synapses in wild type and knockout mice in an electron microscope (Fig. 4B). We analyzed synapse diameter, active zone length, amounts of synaptic vesicles, and abundance of synapses. Strikingly, we did not encounter any differences in synapse morphology between the two groups neither in the RSC nor in the PFC (data not shown). Combined, we demonstrated that expression of synaptic markers was highly oxygen dependent and was down-regulated after loss of HIF-2α, whereas no differences in morphology of synapses were obvious in vivo.
HIF-2α alters neurogenesis pathways during development
As HIF-2 is a transcription factor, we employed a neurogenesis pathway-focused RT2 Profiler Array to screen for changes in expression profiles of neurogenesis pathway genes. Spheres of wild type and knockout animals were differentiated for 24 h under hypoxia prior to mRNA isolation. The profiling hits were based on predefined criteria including gene alterations below p-value < 0.05 and fold changes (fc) larger than ± 2 (Supplemental Table s1). The array showed gene expression alterations in the knockout neurospheres for 9 genes, i.e. Adenosine A2a receptor (Adora2a, fc = 4.19), B-cell leukemia/lymphoma 2 (Bcl2, fc = 2.05), Chemokine (C-X-C motif) ligand 1 (Cxcl1, fc = 2.88), Glial cell line derived neurotrophic factor (Gdnf, fc = 2.48), Neurogenic differentiation 1 (Neurod1, fc = 4.58), Neuropilin 2 (Nrp2, fc = 2.00), Oligodendrocyte differentiation factor 2 (Olig2, fc = 2.48), Paired box gene 3 (Pax3, 3,79), and POU domain, class 4, transcription factor 1 (Pou4f1, fc = -2.23). Additionally, Cyclin-dependent kinase 5, regulatory subunit 1 (Cdk5r1, fc = 1.56), Doublecortin (Dcx, fc = 1.63), Glutamate receptor, ionotropic, Nmda 1 (Grin1, fc = 1.71), Noggin (Nog, fc = 1.61), Notch gene homolog 1 (Notch1, fc = 1.50), SRY-box containing gene 2 (Sox2, fc = 1.66), and Tenascin R (Tnr, fc = 1.73) were identified as genes of interest for further evaluation (Fig. 5A). We subsequently conducted RT-qPCR validation on individual samples from normoxic and hypoxic wild type and knockout spheres on profiling hits from pooled array samples (Fig. 5B). Overall, the expression analyses demonstrated that only Cxcl1 was up-regulated under hypoxia compared to normoxia, although the knockout samples were not significantly different to wild type spheres due to high standard deviations. A down-regulation under hypoxia was found in Bcl2, Grin1, Notch1, Olig2, and Sox2 (only in wild type spheres). Interestingly, Adora2a, Cdk5r1, Gdnf, Pou4f1, and Tnr were significantly up-regulated in the knockout samples compared to wild type controls under normoxia, but not under hypoxia. Whereas Neurod1 and Nrp2 were up-regulated in knockout samples under normoxia as well as under hypoxia, though Neurod1 reached significance only under hypoxia. Additionally, we analyzed expression of Hif-1α and the Wingless/Integrated (Wnt) pathway genes Wnt7a and Wnt7b that are key to oligodendrocyte precursor cell (OPC) maturation arrest under hypoxia16. Hif-1α mRNA was significantly increased in knockout cells and down-regulated upon hypoxia, whereas expression in wild type sample was unaffected. Wnt7b was decreased in knockout samples under normoxia as well as under hypoxia, whereas Wnt7a was not altered (Fig. 5C). Combined, we found that loss of HIF-2α leads to alterations of neurogenic pathway genes during development, especially under normoxic conditions.
Our study revealed the presence of HIF-2α in the adult brain of mice even under normoxic conditions. Deficiency of HIF-2α during brain development lead to a loss of pyramidal cells in the RSC, accompanied by severe impairment in learning and memory. Studies in neural stem cells to unravel the underlying mechanisms showed that the loss of pyramidal cells in the RSC was caused by deficits in migration capabilities in Hif-2-/-mice due to altered expression patterns of genes highly associated with neuronal migration and positioning.
HIF-1 has been found to play a crucial role during brain development, because loss of HIF-1α during development leads to a hydrocephalus accompanied by reduced neuronal cells and impaired spatial memory9. While we found HIF-1α positive cell only under hypoxia, HIF-2α already accumulated under normoxic conditions in the adult mouse brain. HIF-2α is known to become stable at higher oxygen levels than HIF-1α in vitro17 and has been shown to be active under mild hypoxia18 (~ 5% O2). Hence, HIF-2α appears to drive responses at more moderate levels of hypoxia or even normoxia and in forms of long-lasting hypoxia19. Normal oxygen levels in the brain lie between 11.4 and 53.2 mmHg17, which is exactly the range in which one would expect HIF-2α to be stable. Conversely, HIF-1α is most active during short periods of intense hypoxia20. Therefore, we hypothesize that HIF-1 protects the brain in cases of sudden hypoxia, whereas HIF-2 is essential for normal brain function and development. Interestingly, in a study by Wiesener and colleagues HIF-2α protein in the brain of rats was only found after treatment with 0.1% carbon monoxide to cause anemic hypoxia21. The reason for this discrepancy with our data is unclear but might be due to species-specific or methodological differences and needs to be investigated in the future.
To test this hypothesis, we bred mice with a neuro-specific Hif-2α knockout. These animals showed no obvious neurological defects. However, histological analysis revealed a reduced number of predominantly pyramidal cells in the RSC after loss of HIF-2α (Fig. 2). The RSC is a member of a core network of brain regions responsible for a range of cognitive functions, including episodic memory, navigation, imagination, and future planning. In this context the RSC interacts with the hippocampal formation, the para-hippocampal region, including the entorhinal cortex, and thalamic nuclei14. Recently, it was shown that after RSC removal in macaque monkeys, postoperative retention tests revealed a significant retrograde memory loss compared to control animals22. Accordingly, functional MR imaging studies of humans demonstrated that the RSC is also activated when participants are asked to recall autobiographical memories23,24. Here, we found that the loss of pyramidal cells in the RSC was associated with significant impairment in learning and memory as detected by the NOR test (Fig. 2). A recent study on the role of astrocytic HIF illustrates that the loss of HIF-2α in astrocytes leads to disturbances of long-term potentiation (LTP) in acute hippocampal slices, which has been connected with learning and memory12. Interestingly, this effect was only observed under normoxic conditions but not after hypoxic exposure, also indicating a role of HIF-2α during normoxia.
To investigate the cause of less neurons in the RSC, we employed the neurosphere assay as an in vitro model for brain development, and assessed proliferation, migration, differentiation, and apoptosis15. In this context, we encountered impaired migration capabilities in Hif-2α knockout spheres, especially under normoxic conditions. Moreover, we observed a moderately, but significantly reduced number of neurons in the migration area of knockout neurospheres under normoxia which could be caused by reduced migration capabilities of HIF-2α deficient neurons. While it is known that HIF-1α is involved in motility of various cells types, including microglia25, neural crest cells26, and neural progenitor cells27, a role for HIF-2α in cell motion is so far unknown. Our data showing a reduction in migration distance of around 30% due to loss of HIF-2α suggest that the specific loss of neurons in the RSC is caused by insufficient invasion into this brain area.
Dozens of functionally distinct areas exist across the cortex and differences between these functional areas are hypothesized to emerge from a molecular protomap along the germinal zone during neurogenesis28. To elucidate the reason for the specific migration deficit of neurons into the RSC, we screened for gene expression alterations caused by loss of HIF-2α. We could demonstrate that several genes were altered in knockout neurospheres, especially under normoxic conditions (Fig. 5). All these genes play a role in neural migration and patterning. A recent study by Nowakowski and colleagues showed spatiotemporal expressions of ADORA2A, which is strongly expressed in the PFC compared to cortical area V1, and NEUROD1, which is heterogeneously activated during radial glia diversification, indicating a role in migration and positioning of neurons for both genes29. GDK5R1 is a neuron-specific activator of the cyclin-dependent kinase 5 (GDK5) that is required for invading neurons to reach their final position during brain development30. GDNF signaling via GFRalpha1 was shown to promote the differentiation of ventral precursor cells into GABAergic neurons, enhancing their neuronal morphology and motility31. A study by Ng et al. points to a new role for NRP2 in the positioning of neurons during adult hippocampal neurogenesis32. POU4F1 (also known as BRN3A) knockout mice show a loss of neurons in the trigeminal ganglia, the medial habenula, the red nucleus, and the caudal region of the inferior olivary nucleus but not in the retina and dorsal root ganglia33. TNR is an extracellular matrix glycoprotein that is restricted to the central nervous system, where it acts as a multifunctional and versatile molecule. It was reported that the spatiotemporal distribution of TNR parallels neuronal migration34. According to the protomap hypothesis, molecular differences among progenitor cells subdivide the emerging cortical sheet into distinct areas. The extent to which transcriptomic differences establish distinct laminar and areal patterns is difficult to determine and needs to be determined further in future.
Synaptic transmission accounts for up to 50% of cerebral oxygen consumption. Although highly sensitive to changes in oxygen levels, it still remains to be elucidated how hypoxia affects synaptogenesis. Here, we showed that expression of synaptic markers is highly oxygen dependent and diminished upon loss of HIF-2α in vivo and in vitro even under normoxic conditions (Fig. 2E and Fig. 4A). Changes in expression patterns after hypoxia were also observed in other studies. A simple chronic hypoxia model of the snail Lymnaea stagnalis showed repressed Syntaxin-1 (a membrane-bound presynaptic protein) and elevated Vesicle-associated membrane protein-1 (Vamp-1, a vesicle-bound presynaptic protein) levels35. Perinatal hypoxia in rats on postnatal day ten (P10) led to impaired performance in long-term spatial learning and memory (as determined on P45) associated with decreases in the expression of the complex of PSD95 with NMDAR subunits36. Intermittent umbilical cord occlusion in calves resulted in a decreased immunoreactivity of SYN1 in the brain of preterm animals, which indicates decreased presynaptic vesicle formation37. So far, we were unable to detect morphological alterations of synapses in the HIF-2α-/- mice (Fig. 4B). Whether HIF-2α is involved in synapse formation or regulation and if this is involved in the deficits of learning and memory needs to be investigated in the future.
The cell membrane of oligodendrocytes forms myelin sheaths providing functional and trophic support for axons in the white matter of the central nervous system. Yuen and colleagues showed in an elegant study that oxygen tension, mediated by HIF function, is an essential regulator of postnatal myelination, and that hypoxic treatment leads to a reduction of myelin formation due to an OPC maturation arrest16. In this study we confirmed the expansion of myelin was greatly reduced in hypoxic and severely hypoxic conditions (Fig. 3F).We assume that this was likely caused by a reduction in Olig2 expression under hypoxia (Fig. 5), an important factor in oligodendrocyte development. Immunocytochemistry revealed that reduced Mbp expression was accompanied by a reduction in Cnp expression and in O4+ (pre-)oligodendrocytes. These results contradict the findings of Yuen et al. as they showed a maturation arrest without altered total oligodendrocyte lineage numbers16. However, we proved that the loss of HIF-2α leads to a reduction of MBP formation in vivo and in vitro even under normoxic conditions (Fig. 2B and 2E, Fig. 3F), although the numbers of pre-oligodendrocytes & mature oligodendrocytes were comparable in both genotypes (Fig. 3I). The loss in MBP protein might be an alternative explanation for the deficits in learning and memory in the Hif-2α knockout mice. However, a thorough analysis of Mbp heterozygous mice that show a strong reduction in Mbp expression, showed no differences in learning and memory38.
It was shown that Wnt signaling inhibits OPC maturation during development in health and disease16,39. Yuen et al. showed that constitutive HIF-1α/2α stabilization results in OPC maturation arrest through autocrine activation of canonical Wnt7a/7b16. Here, we found a reduced expression of Wnt7b after loss of HIF-2α, whereas Wnt7a was unaffected, indicating that Wnt7b is likely controlled by HIF-2α and Wnt7a by HIF-1α. Further studies are required to investigate the specific roles of the HIF isoforms in oligodendrocyte development.
Interestingly, all genes we showed to be altered in the Hif-2α-/- deficient mice are associated with Alzheimer disease (AD) and memory loss. A gene-based association analysis identified ADORA2A associated with hippocampal volume in mild cognitive impairment and AD40. GDNF is down-regulated in post-mortem middle temporal gyrus of AD patients41, and GDNF administration can protect against AD-like changes induced by injection of aluminum complexes in rabbits42. GDK5R1 activates GDK5 that contributes to the pathophysiology of AD. CDK5 dysregulation facilitates extracellular deposition of Aβ in senile plaques and intracellular accumulation of hyperphosphorylated Tau protein in neurofibrillary tangles43. Directed expression of NEUROD1 in cycling hippocampal progenitors rescued memory loss in an APP x PS1 mouse model of AD44. NRP2 knockout mice showed striking impairments in learning and memory45. Low-level lead exposure led to spatial learning deficits in rats due to reduced protein and mRNA levels of POU4F146. TNR was found to be implicated in AD in a genome-wide association study47. WNT7B is down-regulated in the entorhinal cortex and the hippocampus of AD patients48. Finally, Hif-2α itself is one of the genes that is down-regulated during AD49. Especially striking is the discovery that the earliest metabolic decline in AD is centered on the RSC50, an area, which we showed to be specifically vulnerable to the loss of HIF-2α. Of note, native Tibetans in the Qinghai-Tibetan plateau, who show a special Hif-2α gene polymorphism playing a key role in high altitude adaptation51, have one of the lowest prevalence of AD in the world52.
Our study provides new evidence of an important function of HIF-2α in normal brain function and during development. Genes involved in neuronal migration and positioning need to be orchestrated in a highly specific spatiotemporal manner during development. The hypoxia-inducible factor as a transcription factor conducts several hundred target genes during brain development and maintains normal function of the adult brain by reacting to changes in tissue oxygen tension. Moreover, a role for HIF-2α during normal brain aging and especially during pathological degeneration needs future investigation as hypoxia is one of the key components in the pathophysiology of stroke, Parkinson's or Alzheimer's disease.
Inbred C57BL/6 J mice with loxP sites flanking exon 2 of the Hif-2α gene (Hif-2α+f/+f, purchased from The Jackson Laboratory, Bar Harbor, ME, USA) were crossbred with mice with a NESTIN (NES) promoter driving CRE recombinase (Hif-2α+f/+f x Nes-Cre) to achieve a neural specific Hif-2α knockout. Exon 2 encodes for the DNA binding site of translated HIF-2α protein. Littermates negative for CRE recombinase (HIF-2α+f/+f) served as control animals. Drinking water and standard rodent pellets were provided ad libitum. Wild type and knockout animals demonstrated physiological habitus and bred regularly. Animal breeding was performed in full accordance with the German law for animal welfare and with institutional regulations for animal breeding and handling and approved by the State Agency for Nature, Environment and Consumer Protection North Rhine-Westphalia (file reference: 84–02.04.2016.A173).
Murine progenitor cells were isolated from the cortex of postnatal (P0) wild type and Hif-2α-/- mice and cultivated as neurospheres. Brains were dissected and transferred to minimal essential medium (MEM; Thermo Fisher, Waltham, MA, U.S.A.). Meninges, hippocampi, and olfactory bulbs were removed, and the cerebral cortices were isolated. Subsequently, the tissue was enzymatically digested with 30 U/mL papain (Worthington, Freehold, NJ, U.S.A.) for 20 – 30 min at 37° C to obtain single cell suspensions. Enzyme activity was stopped by adding 1 ml of ovomucoid [1 mg/ml trypsin inhibitor (Merck, Darmstadt, Germany), 50 μg/ml BSA, and 40 μg/ml DNaseI (Worthington) in MEM). After centrifugation at 1000 g for 5 min, cell pellets were resuspended in neurosphere medium consisting of DMEM/F-12 (1:1, Thermo Fisher) containing 0.2 mg/ml l-glutamine (Merck, Darmstadt, Germany) and 2% v/v B27 supplement (Thermo Fisher), 100 U/ml penicillin, and 100 μg/ml streptomycin (both Merck). Cells were allowed to form free-floating spheres at 37° C in 20.9% O2/5% CO2 at a density of 105 cells/ml in T25 flasks (bulk culture) in neurosphere medium in the presence of 20 ng/mL epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF, Preprotech), Every 2–3 days half of the culture medium was changed and supplemented with 20 ng/mL EGF and bFGF. After 7 days in vitro (div) spheres were used for further experiments.
For proliferation analyses individual spheres were cultivated in 96-well plates (bulk culture) in neurosphere medium containing 20 ng/mL EGF and bFGF and incubated under normoxia (20.9% O2), hypoxia (1% O2), and anoxia (0.2% O2). Sphere diameter was measured on days 0, 3, and 7 to determine proliferation, as the diameter correlates directly to the cell number inside the sphere15. Moreover, to define the number of proliferating cells, cells were labelled with CellTiter-Blue assay (Promega, Madison, WI, USA) according to manufacturer’s instructions, also on days 0, 3, and 7.
Migration and apoptosis assay
Migration and differentiation was initiated by mitogen withdrawal. Therefore, spheres were plated in 4-well dishes (Greiner Bio One, Kremsmuenster, Austria), coated with 10 μg/ml poly-ornithine and 10 μg/ml laminin-1 (Merck) in neurosphere medium containing 1% v/v FCS (Merck). Migration distance was assessed after 24, 48, and 72 h under normoxic, hypoxic or anoxic conditions by measuring the distance from the edge of the sphere to the farthest migrated cells at four defined positions per sphere.
Subsequently, the number of dead cells was determined by double staining cells with 2% w/v ethidium bromide (Merck) and 0.1 µg/mL Hoechst 33,258 (Merck) for 2 min. For analysis, spheres were examined using a fluorescence microscope (Axiovert 200 m, Zeiss Jena, Germany) and stained cells were counted manually in relation to the total number of nuclei in the field of view.
After differentiation for 24, 48, and 72 h under normoxic, hypoxic, and severely hypoxic conditions, cells were fixed in 4% w/v paraformaldehyde (Merck) for 15 min at room temperature. Subsequently, immunocytochemistry was performed as previously described with various antibodies15 (see Table 1).
Polymerase chain reaction
Total RNA was isolated from murine brains and neurospheres with the RNeasy Mini Kit (Qiagen, Hilden, Germany) or the NucleoSpin RNA kit (Macherey–Nagel, Dueren, Germany). RT-PCR was performed as previously described with SYBR green fluorescent dye (Eurogentec, Verviers, Belgium) and the iQ5/C1000 Real-time PCR Detection System (Bio-Rad Laboratories GmbH, Munich, Germany). Amounts of complementary DNA were amplified with gene specific primers (see Table 2) and normalized to ribosomal protein (Rsp16) or 60S ribosomal protein L13a (Rpl13a) as indicated. Expression was calculated with the 2-ΔΔCT method.
Genomic DNA for genotyping was isolated from murine tissue with the DNeasy Blood and Tissue Kit (Qiagen) according to the manual, and conventional RT-PCR was performed.
The Qiagen Profiler RT2 Array was performed according to manual. Briefly, wild type and knockout neurospheres were differentiated for 24 h under atmospheric conditions (20.9% O2). Subsequently, RNA was isolated and array was performed.
Brain hemispheres of P70 mice were isolated, fixed in 4% paraformaldehyde, embedded in paraffin, and cut on a microtome (Thermo Fisher). Resulting 2 µm sections for immunohistochemistry and 7 µm sections for conventional histology were H.E. or Nissl stained and microscopically analyzed to determine morphological differences (i.e. cortex thickness at thickest point) between wild type and knockout mice. For further evaluation, immunohistochemical stainings were implemented. We applied the diaminobenzidine method with various antibodies (see Table 1). As immunoperoxidase detection system we used the Vectastain ABC-Kit (Vector Laboratories Ltd). HIF-1α and HIF-2α immunostaining was accomplished using the CSA-I Kit (DAKO).
In situ hybridization
RNA in situ hybridization was performed on 4 µm thick FFPE sections of mouse brain using the RNAscope 2.5 HD Assay-brown (Advanced Cell Diagnostics) according to user manuals 322,452-USM and 322,310-USM using standard conditions. Hif-1α RNA was detected by RNAScope probe Mm-Hif1a (Cat No. 313821), Hif-2α by Mm-Epas1 (Cat No. 314371). For quantification we applied the RNAscope SpotStudio v1.0 Software (Advanced Cell Diagnostics).
For EM studies of synapses, the skull of P70 mice was opened after decapitation and brains were directly fixed in the skull using 2.5% glutaraldehyde in PBS (Merck). The RSC region and the prefrontal cortex were cut into 1 mm thick sections and transferred into 2.5% glutaraldehyde in PBS for 4 h for further fixation. Cubes (1 × 1x1 mm) of the RSC and PFC were cut and post-fixed with 1% osmium tetroxide,dehydrated through a graded series of ethanol, and embedded in Epon 812 (Shell). On a Reichert-Jung ultramicroscope 60 nm ultrathin sections were cut and placed on a copper grid. After staining with 1% uranyl acetate and 0.4% lead citrate sections were examined and digitally acquired on a transmission electron microscope (JEM 1400 Plus; JEOL, Tokyo, Japan).
For behavioral studies, P70 mice were subjected to different tests with at least 48 h break between tests. All tests took place in a quiet, uncolored room. Animal experiments were performed in full accordance with the German law for animal welfare and with institutional regulations for animal breeding and handling and approved by the State Agency for Nature, Environment and Consumer Protection North Rhine-Westphalia (file reference: 84–02.04.2016.A173).
The novel object recognition test was used to evaluate the memory, the affinity to the unknown, the integration of different brain regions, and learning abilities. Testing was carried out in the home cage, therefore the habituation phase was omitted. Each mouse was shortly removed from its home cage, while two identical objects were positioned in the cage, and gently placed back, with the nose facing the middle point of the wall away from objects and let explore for 5 min. After a 30 min retention interval, one of the objects was replaced with a novel object and the mouse was placed in a similar manner in the cage for the 5 min exploration session. Time spent with the familiar and novel object was recorded and the discrimination and recognition ratio for the testing session was calculated for each mouse using the following formula:
Motor skills were examined on the Rotarod (RotaRod Advanced, TSE Systems GmbH, Germany). Rotation speed accelerated from 4 to 20 RPM. The experiment was stopped as soon as all four paws of the mouse touched the ground and fall latencies were measured.
To explore the edge between anxiety and curiosity the black/white-box test was employed. Each mouse was placed in the light chamber facing the glass front and its back to the dark chamber. During 5 min exploration time the following parameters were measured: period until first entrance into dark chamber, total time spent in the light chamber, and number of crossings from light to dark chamber.
For HIF-1α protein stabilization, mice were exposed to a hypoxic environment. Therefore, oxygen concentration was gradually decreased in a hypoxic chamber (Baker Ruskin, Sanford, ME, USA) from normoxia (20.9%) to 10% oxygen concentration (2% down every 2 h). After 6 h at 10% O2, mice were euthanized and brains were isolated and fixed in the hypoxic chamber.
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We kindly thank Professor Karl-Heinz Jöckel (IMIBE, University Hospital Essen, Essen, Germany) for the help with the statistical analyses. We thank Dorothea Schünke from the Imaging Centre Essen (IMCES) for her technical support. TEM images shown in this publication were acquired on IMCES instruments.
Open Access funding enabled and organized by Projekt DEAL.
The authors declare no competing interests.
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Kleszka, K., Leu, T., Quinting, T. et al. Hypoxia-inducible factor-2α is crucial for proper brain development. Sci Rep 10, 19146 (2020). https://doi.org/10.1038/s41598-020-75838-4