Table 2 Primers sequences used for constructing luciferase vector.

From: Cir-ITCH inhibits gastric cancer migration, invasion and proliferation by regulating the Wnt/β-catenin pathway

Vector Forward primer Reverse primer
Wild type of cir-ITCH CCGCTCGAG-TTGAAGAAGTAGTTGTGACTTTGC ATTTGCGGCCGC-CCCATCCAGGTGGCAATGGACCAA
Mutant cir-ITCH for miR-17 AATGGTGAAA-gacatca-TTCAGAAAGTG CACTTTCTGAA-tgatgtc-TTTCACCATT
Mutant circITCH for miR-214 CCTCTTA-gactacctgc-AGGCTCAGGCCCTAG CTAGGGCCTGAGCCT-gcaggtagtc-TAAGAGG
Wild type 3′UTR of linear ITCH CCGCTCGAG-CTTCTGAGAACTTGCACCATGAAT ATTTGCGGCCGC-GAATCACTTATGTCTTTAGAT
Mutant type 3′UTR of linear ITCH TTATTAACTGATTAAT-tagcagta-AAGTTCTCTGG CCAGAGAACTT-tactgcta-ATTAATCAGTTAATAA