Table 1 Primers sequences used for qRT-PCR.
| Gene | Forward primer | Reverse primer | Probe |
|---|---|---|---|
| cir-ITCH (Taqman) | GCAGAGGCCAACACTGGAA | TCCTTGAAGCTGACTACGCTGAG | CCGTCCGGAACTATGAACAACAATGGCA |
| GAPDH (Taqman) | CCATGACCCCTTCATTGACC | TTGATTTTGGAGGGATCTCG | CTGAGAACGGGAAGCTTGTC |
| Linear ITCH | TAGACCAGAACCTCTACCTCCTG | TTAAACTGCTGCATTGCTCCTTG | |
| Linear ITCH1 | GGAGACAACGCCTTAACC | CATCTACTGTGACCTCTACG | |
| Linear ITCH2 | CAACAGAGACAATAGGAGACT | TCAGAAGTGGCAGATGGT | |
| Linear ITCH3 | GATAACATCCAGTAACCACAG | ATAACAGCCACCTTGACAG | |
| Linear ITCH4 | GGCAGCACTGGATTGTATA | GCAACCACTGAAGGAACT | |
| Circular ITCH | ACAAGAACAACAACGTGGCA | GCTCTTTGTCACCTCCAAGC | |
| c-Myc | TTCGGGTAGTGGAAAACCAG | CAGCAGCTCGAATTTCTTCC | |
| CyclinD1 | GAGGAGCAGCTCGCCAA | CTGTCAAGGTCCGGCCAGCG | |
| GAPDH | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC | |
| miR-216b | ACACACTTACCCGTAGAGATTC | Universal primer | |
| miR-17 | CAAAGTGCTTACAGTGCAGGTA | Universal primer | |
| miR-214 | ACAGCAGGCACAGACAGGCAGT | Universal primer | |
| miR-7 | TGGAAGACTAGTGATTTTGTT | Universal primer | |
| miR-128 | TCACAGTGAACCGGTCTCTTT | Universal primer | |
| U6 | GGGCAGGAAGAGGGCCTAT | Universal primer |
