BRCA1 and BRCA2 genes mutations among high risk breast cancer patients in Jordan

Familial breast cancer is estimated to account for 15–20% of all cases of breast cancer. Surveillance for familial breast cancer is well-established world-wide. However, this service does not exist in Jordan, due to the scarcity of information with regard to the genetic profiling of these patients, and therefore lack of recommendations for policy-makers. As such, patients with very strong family history of breast or ovarian cancers are not screened routinely; leading to preventable delay in diagnosis. Whole coding sequencing for BCRA1/BCRA2 using next-generation sequencing (NGS)/Ion PGM System was performed. Sanger sequencing were then used to confirm the pathogenic variants detected by NGS. In this study, 192 breast cancer patients (and 8 ovarian cancer cases) were included. The prevalence of recurrent pathogenic mutations was 14.5%, while the prevalence of newly detected mutations was 3.5%. Two novel pathogenic mutations were identified in BRCA2 genes. The common mutations in the Ashkenazi population used for screening may not apply in the Jordanian population, as previously reported mutations were not prevalent, and other new mutations were identified. These data will aid to establish a specific screening test for BRCA 1/BRCA2 in the Jordanian population.


Scientific Reports
| (2020) 10:17573 | https://doi.org/10.1038/s41598-020-74250-2 www.nature.com/scientificreports/ will lead to directed testing of high-risk subpopulations, and therefore earlier diagnosis and superior management leading to better outcomes. However, there is no founder mutation(s) in BRCA1/BRCA2 in the Jordanian population that could be used for early genetic screening. In this study, the authors evaluated the potential contribution and the frequency of mutations in the BCRA1/BCRA2 genes in selected Jordanian patients with breast and ovarian cancers, identified based on NICE criteria for high risk of familial cancer, through performing whole coding sequence for BCRA1/ BCRA2 with the purpose of providing baseline data for establishment of familial cancer services in Jordan.

Materials and methods
Ethical approval and consent to participate. The study was approved by the Institutional Review Boards and Ethics Committees at Mutah University and the Central Ethics Committee at the Ministry of Health, Jordan. All included patients were informed about the objective and protocol of the study and gave written informed consent in accordance with the Declaration of Helsinki prior to undergoing any procedures. Study population. This prospective cross-sectional study was conducted et al. Bashir Hospital, Amman, Jordan. All Jordanian breast cancer patients referred to this hospital during the study period were under surveillance for familial breast or ovarian cancer according to a uniform selection criterion (see below).The U.K. National Institute for Health and Care Excellence (NICE), November 2013 Clinical guidelines for identification of patients with high risk for familial breast/ovarian cancer who required referral for testing in a specialist genetics clinic were used for identifying study participants and labelling them as 'high risk for familial cancer 14 .
Patients files were reviewed for clinical and pathology data such as stage, grade, site, laterality, oestrogen receptor status, HER2 (or HER2 neu) positive status, etc.
Sample collection and genomic DNA isolation. Peripheral blood samples were drawn from study participants (192 breast cancer patients and 8 ovarian cancer cases) and sent for analysis at the Cell Therapy Centre at Jordan University. Genomic DNA was extracted from 5 ml EDTA tubes using QIAprep Spin Miniprep Kit according to manufacturer's recommendations, and quantified using Nano Drop Spectrophotometer (Thermo Fisher Scientific).

Next-generation sequencing and variant analysis. BRCA1/BRCA2 library preparation and Ion PGM
next-generation sequencing. The coding exons and flanking intronic regions of the BRCA1/BRCA2 genes were amplified using Ion AmpliSeq Library Kit 2.0 (Life Technologies) and Ion AmpliSeq BRCA1/BRCA2 Panel (Life Technologies). The whole process was performed following the manufacturer's instructions with minor modifications as previously described 15,16 . Each library was barcoded using Ion Xpress Barcode Adapters kit (Life Technologies) and its concentration was determined with Ion Library TaqMan Quantitation Kit (Life Technologies). For template preparation, emulsion PCR and enrichment were carried out using Ion OneTouch 2 system and the Ion OneTouch ES Instrument (Life Technologies) with Ion PGM Hi-Q View OT2 Kit (Life Technologies). An average of six prepared barcoded samples were loaded into a single Ion 316 Chip v2 (Life Technologies) per sequencing run. The sequencing reactions were performed in the Ion torrent PGM System (Life Technologies) using sequencing Ion PGM Hi_Q view sequencing kit (Life Technologies). The generated data on Ion PGM were processed through on Torrent Suite, version 5.0.4, running on a local server (Life Technologies). The VCF files were uploaded to Ion Reporter (Life Technologies) and annotated for single-nucleotide variants (SNV), insertions and deletions (indels), and splice site changes. Reads were visualized with the Integrative Genomics Viewer (IGV) and Alamut (Interactive Biosoftware, Rouen, France).

Evaluation of the variants clinical significance. Variants that are clearly classified in the Breast Cancer
Information Core (BIC) database, Human Gene Mutation Database Pro (HGMD pro) and ClinVar databases, and not previously described as pathogenic in the literature were evaluated based on the guidelines and standards for interpretation of sequence variants recommended by the American College of Medical Genetics and Genomics (ACMG) 17  Overall BRCA1/BRCA2 mutations. The overall prevalence of recurrent and novel mutations (pathogenic and variants of uncertain significance (VUS)) in BRCA1/ BRCA2 genes was 18% ( Table 2). The prevalence of recurrent pathogenic mutations was 14.5%, while the prevalence of novel mutations (pathogenic and VUS) was 3.5%. Two confirmed novel pathogenic mutations were identified in BRCA2 genes. Regarding site of mutation, 7.5% (15 patients) had pathogenic mutations in BRCA1 gene and 7% (14 patients) had pathogenic mutations in BRCA2 gene. Five VUS novel variants in BRCA2 gene were identified in this study. Regarding previously reported VUS, this study detected 6 in BRCA1 genes and 9 in BRCA2 genes ( Table 3, Supplementary table 2). Figure 1 shows mutations in BRCA1/BRCA2 according to patients' selection criteria. Participants with relatives with ovarian cancer had higher prevalence of mutations compared with other groups. Regression analysis results showed that E cadherin, PR, ER, oestrogen receptors status, laterality and recurrence since baseline were the statistically significant predictors of the presence of BRCA1 mutations. On the other hand, E cadherin, laterality and recurrence since baseline were statistically significant predictors of BRCA2 mutations.  Table 3, six BRCA1 variants were pathogenic, two of them were nonsense, one frameshift and three missense variants. In addition, six BRCA1 variants were VUS; four of them were missense variants, one VUS was synonymous, and another was a new splice site change (c.5396-6 T > C) that was present for the first time in our breast cancer patient (Fig. 2, Supplementary table 2). BRCA2 germline variants are presented in Table 3. Two pathogenic BRCA2 variants (p.K2075S fs*2; c.6224_6225delAA and p.Q2899R; www.nature.com/scientificreports/ c.8696A > G) were novel, they are neither registered in the disease nor the population databases. Those two novel pathogenic variants were further validated by Sanger sequencing (Supplementary Fig. 1 a-b). Four others recurrent BRCA2 pathogenic variants were identified. They were registered in diseases databases but absent from the population databases gnomAD indicating they are rare variants ( Fig. 2 and Table 3). The duplication variant in BRCA2 (p.N1784K fs*3; c.5351dupA) was in a homopolymer region and therefore we validated it through Sanger sequencing (Supplementary Fig. 1c).We also found five distinct novel variant in BRCA2 and classified them as VUS (p.S958R; c.2874 T > G, p.I1000L; c.2998A > C, p.H1525L; c.4574A > T, p. = (p.P2883P), c.8649A > T, p.T3204I; c.9611C > T). In addition, our cohort had nine variants that were classified as VUS (Supplementary  table 2). However, the founder variants of BRCA1 (c.185delAG, BRCA1 c.5382insC), and BRCA2 (c.6174delT) in the Ashkenazi Jewish population, were absent in the current cohort, indicating that BRCA variants vary among different populations 18 .

Discussion
This study was set out to identify BRCA1/BRCA2 mutations in patients at high risk of familial breast and ovarian cancer using a systematic approach. Our study showed a high prevalence of 18.0% for positive mutations in BRCA1 (7.5%) or BRCA2 (10.5%) genes. Two novel pathogenic mutations and five novel VUS variants were identified in this study, in addition to identifications of patients with recurrent pathogenic and VUS mutations. These figures are higher than recent data from neighboring Arab Peninsula of 16% or from other Asian countries like Japan of 7% [15][16][17][18][19] . This could be related to our inclusion criteria based on NICE guidelines for being at high risk of familial breast or ovarian cancers. Out of 12 pathogenic variants detected in this study in both BRCA1/BRCA2, five pathogenic variants were reported previously among Caucasian, Western populations and Latin American. These variants including c.5186C > A,, c.4065_4068delTCAA in BRCA1 and c.5042_5043delTG, c.5351dupA in BRCA2 [20][21][22][23][24] .Additionally, three pathogenic variants were previously reported among Asia population. Both c.1224delA and c.5224C > T in BRCA1 and c.6634_6637delTGTT in BRCA2 were originally reported in Japan and China populations 25,26 . However, different recurrent pathogenic variants were previously reported among Arab population. The c.121C > T pathogenic variant in BRCA1and the c.2254_2257delGACT pathogenic variant in BRCA2 were recently reported in Palestinian patients 27,28 . Additionally, the c.5158C > T pathogenic variant in BRCA1 was previously reported in Morocco and Saudi Arabia 13 . Both c.6224_6225delAA and c.8696A > G variants in BRCA2 have not been described in the BIC database and other databases including HGMD professional and ClinVar. Therefore, they are considered to be novel. It's worth to mention that four variants in both BRCA1 and BRCA2 were common in more than one patient (Table 3) [29][30][31][32] .
Positive patients in our study (recurrent or novel mutations) were diagnosed at advanced stages of breast cancer and ovarian cancers, although they are at high risk of familial cancer. This preventable delay is expected to have a major negative impact on morbidity, morbidity, and cost of management. Such patients could have received preventive cancer treatment giving their very strong family history, should they have been tested properly in due time. Establishing the mutational profile in our region will allow directed testing of high-risk families, therefore increasing the benefit while reducing costs. This is an agreement with Laitman et al. who identified several highly-recurring mutations in the region 13 .
Our participants presented with breast cancer at an early age with a mean of 43.7 ± 2.7. Breast cancer at an early age is more likely to be associated with an increased familial risk, particularly in women with a germline www.nature.com/scientificreports/ BRCA1 mutation 33 . In a study of women with breast cancer diagnosed before age 30, BRCA1/BRCA2 and TP53 mutations were found in about half who had strong family histories of breast cancer and in less than 10% of women with non-familial breast cancer 34 . A young age at diagnosis for breast cancer patients in Jordan and the Arab world suggests a high probability of familial predisposition. A systematic review on age at diagnosis for breast cancer in the Arab world revealed that average age was 48 (SD = 2.8), range 43-52, median 48.5 and mode 45. These figures are based on data from 28 articles that included years amongst the 7455 patients. The percentage of patients that were younger than 50 years old was reported in 11 articles from 8 countries and included 5144 patients; 65.5% (SD = 11) who were less than 50 years old (range 49-78%, median = 66%). It is recommended that further research to be conducted among young breast and/or ovarian cancer patients in the Arab world to examine the prevalence of known mutations and to search for new potential pathogenic mutations. Identification of mutations in BRCA1/BRCA2 genes leading to familial breast cancer has had a major impact on prevention of breast and ovarian cancer through development of clinical practice guidelines for management and referral systems for women with a personal or family history of breast or ovarian cancer 35 . Results from some countries (e.g. U.K., France and Canada) and specific cities (Manchester, Marseilles, and Montreal) provided excellent examples of the clinical and economic effectiveness of these preventive services 36 . Management of patients start as early as the age of 25 with clear guidelines for referral and management 37 . In many developing countries, including Jordan, this approach has not been put in place 38 . It has been well-reported in previous studies that positive carriers for BRCA1/BRCA2 have high cumulative risk estimates for developing breast cancer reaching 72% for BRCA1 carriers and 69% for BRCA2 carrier by age of 80 5 . These patients could undergo preventive measures following timely identification of mutations, reducing morbidity and mortality arising from breast and ovarian cancers.

Conclusion
This study assessed the prevalence of reported mutations and identified novel mutations for BRCA1/BRCA2 genes. Giving the high prevalence of positive mutations in BRCA1/BRCA2 genes, it is essential to establish a service for familial breast and ovarian cancer service in Jordan and the MENA region, that includes genetic counselling and early genetic screening.

Data availability
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