Table 1 Samples and corresponding DNA extraction strategy.

From: Non-specific amplification of human DNA is a major challenge for 16S rRNA gene sequence analysis

Sample DNA extraction strategy
Breast: tumour and normal Molzym Ultradeep Microbiome (Molzym, Bremen, Germany)
Oesophageal biopsies AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) with modifications10
Skin Swab samples QIAamp UCP Pathogen Mini Kit (Qiagen, Hilden, Germany)
Stool samples Repeated bead beating method as previously described, with modifications11, 12