Post-mitotic cardiomyocytes have been considered to be non-permissive to precise targeted integration including homology-directed repair (HDR) after CRISPR/Cas9 genome editing. Here, we demonstrate that direct delivery of large amounts of transgene encoding guide RNA (gRNA) and repair template DNA via intra-ventricular injection of adeno-associated virus (AAV) promotes precise targeted genome replacement in adult murine cardiomyocytes expressing Cas9. Neither systemic injection of AAV nor direct injection of adenovirus promotes targeted integration, suggesting that high copy numbers of single-stranded transgenes are required in cardiomyocytes. Notably, AAV-mediated targeted integration in cardiomyocytes both in vitro and in vivo depends on the Fanconi anemia pathway, a key component of the single-strand template repair mechanism. In human cardiomyocytes differentiated from induced pluripotent stem cells, AAV-mediated targeted integration fluorescently labeled Mlc2v protein after differentiation, independently of DNA synthesis, and enabled real-time detection of sarcomere contraction in monolayered beating cardiomyocytes. Our findings provide a wide range of applications for targeted genome replacement in non-dividing cardiomyocytes.
Genomic cleavage after DNA double-strand breaks (DSB) induced by CRISPR-mediated genome editing is repaired through the non-homologous end-joining (NHEJ) or homology-directed repair (HDR) pathways1,2,3. In contrast to NHEJ, which is an error-prone process that forms an insertion or deletion at the DSB site, HDR enables accurate genome repair using single- or double-stranded DNA templates. Because HDR primarily occurs during the S/G2 phase, the HDR-based repair process after genome editing is restricted to cells that are actively dividing4,5,6,7, thereby limiting its application to hearts consisting of non-dividing cardiomyocytes that cease to proliferate after birth8. Previously we demonstrated that Adeno-associated virus (AAV)-mediated gene delivery enabled targeted genome replacement via HDR, independently of DNA synthesis, in non-dividing cultured cardiomyocytes9 isolated from Cas9 knock-in mice10. Transduction of AAV6 encoding gRNA and a repair template efficiently introduced the tdTomato sequence into the 3′ end of the Myl2 gene encoding Mlc2v, a ventricular isoform of myosin regulatory light chain, and yielded the sarcomeric Mlc2v-tdTomato fusion protein. Although Mlc2v plays a pivotal role in cardiogenesis and sarcomeric function11,12, the introduction of tdTomato fluorescent protein into the C-terminus of endogenous Mlc2v protein does not significantly affect sarcomere structure9,13, suggesting that Myl2 is a useful and appropriate endogenous target gene to detect HDR or visualize sarcomere contraction in cardiomyocytes. Although our previous findings demonstrated that AAV-mediated gene delivery promoted HDR in cardiomyocytes that had not entered S-phase, these observations were limited to cultured conditions using murine cardiomyocytes in the neonatal stage. Although CRISPR/Cas9-mediated targeted gene disruption via NHEJ has thus far been effective in murine adult heart tissues14, HDR-mediated replacement of the targeted genome in cardiomyocytes of the adult heart has not been reported. Furthermore, the mechanism underlying targeted integration in non-dividing cardiomyocytes remains to be elucidated.
Here we demonstrated that AAV-mediated gene delivery promoted precise targeted integration in murine adult heart tissues and human cardiomyocytes after differentiation from induced pluripotent stem cells (hiPSCs) independently of DNA synthesis, and enabled direct visualization of sarcomeres. In contrast to AAV, adenovirus (AdV) promoted targeted integration only in a limited population of cultured neonatal cardiomyocytes, all of which had entered S-phase, suggesting a distinct underlying molecular mechanism. We clarified that targeted integration promoted in non-dividing cardiomyocytes was mediated by the recently identified Fanconi anemia pathway involved in single-strand template repair (SST-R)15. Our findings uncover the essential role of AAV-mediated gene delivery in promoting precise integration in cardiomyocytes and suggest a wide range of applications for targeted genome replacement.
AAV-mediated gene delivery promotes precise targeted integration in adult cardiomyocytes
To induce HDR in cardiomyocytes, we previously generated AAV6 encoding gRNA targeting the 3′ end of the mouse Myl2 gene and repair template DNA encoding tdTomato fluorescent protein flanked by homology arms (Fig. 1A). The generated vector efficiently replaced the target genome and yielded C-terminally tagged Mlc2v-tdTomato fusion protein in cultured neonatal cardiomyocytes9 isolated from Cas9 knock-in mice in which 3xFLAG-fused Cas9 and a P2A self-cleavable peptide followed by EGFP protein were knocked in at the endogenous Rosa26 locus10. In our previous experiments, transduction of AAV6 into cardiomyocytes isolated from murine adult heart tissue by the Langendorff perfusion method failed to induce HDR9, probably because of the low transduction efficiency or the low viability of isolated adult cardiomyocytes after viral transduction. Because AAV9, like AAV6, achieves high transduction efficiency in rodent hearts16, we generated AAV9 encoding the same gene components (Fig. 1B), and confirmed Mlc2v-tdTomato–positive cardiomyocytes after viral transduction in in vitro conditions (Supplementary Fig. 1A). To promote HDR in in vivo heart tissue, AAV6 or AAV9 was systemically injected via the orbital venous sinus into 8-week-old Cas9 knock-in mice. Although the transgenes were detected in myocardium 7 days after injection (Supplementary Fig. 1B), tdTomato-positive cardiomyocytes were not observed in either AAV6- or AAV9-injected mouse hearts (Supplementary Fig. 1C). To increase the copy number of transgenes, we directly injected AAV6 (2.5 × 109 viral genomes) or AAV9 (9.2 × 1010 viral genomes) encoding gRNA and repair template DNA into the left ventricular myocardium of 8-week-old Cas9 knock-in mice. Intriguingly, 5 days after injection, tdTomato-positive cardiomyocytes were observed scattered around the injection site in the left ventricular myocardium of both AAV6- and AAV9-injected mouse hearts (Fig. 1C). Troponin I staining showed that tdTomato fluorescent signals were merged with the sarcomere structure. Intron-spanning PCR using cDNA samples obtained from injected myocardium and Sanger sequence analysis confirmed that the tdTomato sequence was precisely introduced into the 3′-terminus of the Myl2 gene (Fig. 1D and Supplementary Fig. 1D). We evaluated whether AAV mediates precise targeted integration without Cas9 in our experimental conditions, because AAV mediates homologous recombination in the absence of exogenous nucleases17. We directly injected AAV6 encoding gRNA and repair template DNA into the left ventricular myocardium of 8-week-old WT mice. Five days after injection, tdTomato-positive cardiomyocytes were not observed in the dissected tissues (Supplementary Fig. 1E), suggesting that precise integration of tdTomato into the 3′-terminus of Myl2 in adult heart tissues required DSB caused by Cas9. To determine whether genomic replacement actually occurred in cardiomyocytes without S-phase entry in ventricular tissues injected with AAV6, 5-ethynyl-2′-deoxyuridine (EdU)18 was injected to mice 2 h before AAV6 transduction, then injected for 5 consecutive days after transduction (Supplementary Fig. 1F). Five days after AAV6 transduction, EdU-positive non-cardiomyocytes were observed around the injection site (Supplementary Fig. 1G), representing the reactive proliferation of interstitial cells after injury and the successful incorporation of the injected EdU. tdTomato-positive cardiomyocytes were observed around the injection site 5 days after injection, and none of these cells were positive for EdU (Supplementary Fig. 1G). These data suggest that direct injection of AAV induced precise targeted integration in cardiomyocytes in adult murine heart tissues independently of S-phase entry.
A high transgene copy number is required to promote targeted integration
We obtained myocardial tissues 14 and 28 days after direct injection of AAV6. Of note, cardiomyocytes positive for tdTomato fluorescent signals localized at sarcomeres were significantly increased around the injection site at day 14, and these tdTomato-positive cardiomyocytes remained intact until day 28 (Fig. 2A and B). To confirm that Myl2-tdTomato fusion mRNA was transcribed in local tdTomato-positive sections at day 28, total RNA was extracted from tdTomato-positive and -negative micro tissue sections obtained by laser microdissection. Intron-spanning PCR and Sanger sequencing analysis using cDNA samples confirmed precise genome replacement (Fig. 2C and Supplementary Fig. 2A). Confocal microscopy analysis demonstrated that Mlc2v-tdTomato protein precisely visualized sarcomeric A-bands tandemly aligned with I-bands detected by troponin I staining in the heart tissues 28 days after direct injection (Fig. 2D and Supplementary Fig. 2B). These data suggest that Mlc2v-tdTomato fusion protein was gradually replaced with endogenous Mlc2v protein after viral transduction, and did not significantly affect sarcomere structure in the heart in vivo. To determine the copy number of the local transgenes delivered by direct injection of AAV6, genomic DNA was obtained from dissected cardiac sections at day 28, including from tdTomato-positive cardiomyocytes at injected sites and tdTomato-negative cardiomyocytes at remote sites (Supplementary Fig. 2C). Notably, although hemodynamic delivery after direct injection caused the transgenes in remote sites to be detected by PCR analysis at day 28 (Supplementary Fig. 2D), tdTomato-positive cardiomyocytes could not be detected there. Quantitative real-time PCR analysis clarified that the copy number of transgenes in the injected sections containing tdTomato-positive cardiomyocytes were more than 100-fold higher than those in the remote sections (Fig. 2E). These data suggest that Mlc2v-tdTomato fusion protein in cardiomyocytes was robustly expressed 28 days after transduction, and that a high copy number of the transgenes are required to promote targeted integration in adult murine cardiomyocytes in vivo.
Although immunostaining experiments clarified the precise genome replacement in adult heart tissues, the other outcomes including NHEJ-mediated deletion or insertion were also expected. To evaluate this issue, genomic DNA were extracted both from neonatal cultured cardiomyocytes and adult heart tissues of Cas9 knock-in mice treated with AAV6 encoding gRNA and repair template. The targeted DNA sequences were amplified with the primes outside the both 5′- and 3′- homology arms and analyzed by the targeted deep sequencing in the duplicate samples. Both in neonatal cultured cardiomyocytes and adult heart tissues, indel mutations caused by NHEJ were detected around the PAM sequence targeted by the gRNA (Fig. 2F). Deep sequence analysis with extremely high coverage demonstrated that NHEJ and targeted integration (knock-in of tdTomato) was detected in cultured neonatal cardiomyocytes at a rate of 46.9%, 7.1% and 49.4%, 6.39%, respectively. In adult heart tissues, NHEJ and targeted integration were detected at a rate of 1.59%, 0.038% and 0.48%, 0.0099%, respectively (Fig. 2G). Notably, the efficiency of targeted integration compared to NHEJ estimated by deep sequencing were significantly lower in adult heart tissues than in neonatal cultured cardiomyocytes (15.1%, 12.9% in neonatal cultured cardiomyocytes, and 2.4%, 2.1% in adult heart tissues, Fig. 2G).
AdV does not promote S-phase entry-independent targeted integration
AAV is a nonenveloped virus with single-stranded DNA (ssDNA), and imported ssDNA is converted to double-stranded DNA and transcribed19. To determine whether gene delivery as ssDNA is required to promote targeted integration in cardiomyocytes, we used adenovirus (AdV) that deliver transgenes as double-stranded DNA and achieve high transduction efficiency and robust expression of the transgene in cardiomyocytes20. We first validated the ability of AdV to promote targeted integration in cultured cardiac fibroblasts isolated from neonatal Cas9 knock-in mice. AdV encoding gRNA and repair template DNA successfully introduced the tdTomato sequence into the 3′ end of the mouse Actb gene (Supplementary Fig. 3A–E). Next, to investigate whether AdV-mediated gene delivery also promote targeted integration in cardiomyocytes, the gRNA and repair template sequence targeting Myl2 located between the inverted terminal repeat (ITR) sequence (Fig. 1B) was entirely subcloned into the cloning vector to produce AdV. To determine whether AdV-mediated gene delivery also promote targeted integration in adult murine heart tissue, we directly injected AdV into the left ventricular myocardium of 8-week-old Cas9 knock-in mice. Five days after AdV injection, tdTomato fluorescent signals were undetectable (Supplementary Fig. 3F) despite the comparable transduction efficiencies of AdV and AAV6 transgenes in heart tissues (Supplementary Fig. 3G). To further evaluate the characteristics of both viral vectors, we transduced both AAV6 and AdV encoding the same sequence into cultured neonatal cardiomyocytes isolated from Cas9 knock-in mice and performed sequential observations using high-content image analysis. Transduction at the adjusted viral titer led to comparable transduction efficiencies between AAV and AdV as determined by quantitative real-time PCR (Supplementary Fig. 3H). After transduction with AAV6, cardiomyocytes positive for tdTomato fluorescent signals clearly localized in the sarcomere were observed at a rate of 14% at day 5 (Fig. 3A and B), consistent with previous findings9. After transduction by AdV encoding the same DNA sequence, only a limited number of cardiomyocytes positive for tdTomato fluorescent signals localized in the sarcomere were detected, (Fig. 3A, arrowhead), at a rate of 0.04% (Fig. 3B). PCR analysis using genomic DNA and cDNA followed by Sanger sequencing validated the precise integration at the Myl2 gene mediated by both viral vectors (Fig. 3C, D and Supplementary Fig. 3I), although the efficiency of targeted integration was significantly lower in cardiomyocytes transduced with AdV than with AAV, consistent with the immunostaining results. To clarify the characteristics of the limited population of tdTomato-positive cardiomyocytes transduced with AdV, we investigated the association between targeted integration and DNA synthesis in cardiomyocytes. EdU was added to cardiomyocytes 6 h before transduction of AAV or AdV, then cardiomyocytes were continuously labeled for 4 days. Cardiomyocytes were sequentially observed, and the images of immunostained samples fixed on day 4 were quantitatively analyzed using high-content image analysis (Fig. 3E). After 4-day culture, 21% of cardiomyocytes were positive for EdU, suggesting that these cells had entered S-phase at least once (Fig. 3F). AAV-mediated gene delivery uniformly promoted targeted integration both in cardiomyocytes that had and had not passed S-phase (Fig. 3E and G), as previously reported9. In contrast, in cardiomyocytes transduced with AdV, the small number of cardiomyocytes positive for tdTomato fluorescent signals that co-localized with sarcomeres were all double positive for EdU (Fig. 3E and G). These data suggest that targeted integration promoted by AdV transduction was restricted in cardiomyocytes that had entered S-phase, whereas AAV-mediated gene delivery promoted targeted integration independently of S-phase entry.
Inhibition of the Fanconi anemia pathway suppresses targeted integration in cardiomyocytes
HDR includes three pathways: homologous recombination (HR), microhomology-mediated end joining (MMEJ), and single-strand template repair (SST-R)21. HR uses homologous sequences of sister chromatids or exogenously provided DNA sequences as repair templates4. MMEJ repairs DSB via short homology sequences containing a few complementary nucleotides22. SST-R repairs DSB using single-strand oligo DNA, which has high efficiency in human cells23. In contrast to the former two pathways, the underlying mechanism of SST-R is poorly understood. A recent study demonstrated that SST-R after Cas9-mediated DSB required the Fanconi anemia (FA) pathway, which is normally involved in the mechanism of repairing interstrand cross-links15. To investigate whether AAV-mediated targeted integration in cardiomyocytes requires the FA pathway, Fanca, a key component of the FA pathway15,24, was knocked down using shRNA (Fig. 4A). Lentivirus-mediated delivery of shRNAs significantly decreased the mRNA expression levels of Fanca in cultured neonatal cardiomyocytes (Supplementary Fig. 4A). As shown in Fig. 4B and C, knockdown of Fanca using two different shRNAs significantly reduced the number of tdTomato-positive cardiomyocytes after AAV transduction. To further determine the in vivo relevance of the FA pathway, we generated AAV6 encoding gRNA that targeted exon 2 of the Fanca gene (Fig. 4D). Transduction of AAV6 into cultured cardiomyocytes isolated from neonatal Cas9 knock-in mice induced NHEJ at the targeted locus (Supplementary Fig. 4B) and significantly decreased Fanca mRNA expression levels (Supplementary Fig. 4C). The myocardium of 8-week-old Cas9 knock-in mice were co-injected with AAV6 encoding Fanca-targeting gRNA and AAV6 encoding gRNA with a repair template targeting Myl2. At day 7 after direct injection, the number of tdTomato-positive cardiomyocytes was significantly decreased in myocardium co-injected with AAV6 targeting Fanca compared to AAV6 targeting LacZ (Fig. 4E and F). BRCA2, also known as FANCD1, is involved in FA pathway via regulation of homologous recombination24, and is required for AAV-mediated homologous recombination in B lymphoblastoid cell lines17. As shown in Supplementary Fig. 4D and E, knock down of Brca2 significantly reduced the number of tdTomato-positive cardiomyocytes after AAV transduction, suggesting that Brca2 as well as Fanca play pivotal roles in the AAV-mediated targeted integration in cardiomyocytes. These data suggest that targeted integration promoted by AAV transduction at least partly depends on an SST-R mechanism mediated by the Fanconi anemia pathway.
AAV-mediated targeted integration is effective in differentiated hiPSC-CMs
Finally, we examined whether AAV-mediated targeted integration is effective in human cardiomyocytes, because a direct method to replace the targeted genome in differentiated, contracting human cardiomyocytes may provide a variety of analytical applications. To this end, we differentiated the hiPSCs generated from a cardiomyopathy patient into cardiomyocytes (hiPSC-CMs) using a 2D monolayer method as previously described25. This yielded approximately 84% troponin T–positive cardiomyocytes at day 10 after differentiation induction (Supplementary Fig. 5A). We designed gRNA targeting the 3′ end of the human MYL2 gene and repair template DNA encoding tdTomato fluorescent protein flanked by the homology arms (Fig. 5A). gRNA was designed in an antisense direction targeting a PAM sequence located 19 bp downstream of the MYL2 stop codon to avoid Cas9-mediated re-cleavage of exon 7. We generated AAV2 encoding a gRNA sequence and repair template, because AAV2 was previously shown to efficiently transduce hiPSC-CMs26, and was found to transduce hiPSC-CMs with high efficiency in our experimental conditions (Supplementary Fig. 5B). The differentiated cardiomyocytes were trypsinized and replated onto a 96-well plate at day 10 after differentiation. These cells were labeled with EdU, then transduced with AAV2 encoding gRNA and a repair template, followed by transfection with mRNA encoding Cas9 (Fig. 5B). Continuous labeling with EdU demonstrated that after replating, approximately 5% of the troponin T–positive hiPSC-CMs entered S-phase during 3-day culture (Supplementary Fig. 5C and 5D). Sequential observation using high-content image analysis detected MLC2v-tdTomato–positive hiPSC-CMs without S-phase entry (Fig. 5C). Precise targeted integration was confirmed by genomic PCR and Sanger sequencing analysis (Fig. 5D and Supplementary Fig. 5E). Based on these results, we directly transduced AAV2 into contracting, monolayered, sheet-like hiPSC-CMs at day 14 (Fig. 5B), then transfected mRNA encoding Cas9. Seven days after transfection, fluorescent-labeled sarcomeres were visible in monolayered hiPSC-CMs, enabling live tracing of the oriented sarcomeric A-bands in hiPSC-CMs (Fig. 5E and Supplementary movie). These data suggest that AAV2-mediated gene delivery combined with transient transfection of Cas9 promoted targeted integration in human monolayered hiPSC-CMs and allowed direct visualization of contracting sarcomeres after differentiation.
Precise genome replacement in non-dividing cardiomyocytes has been challenging because the repair process using HDR is restricted to dividing cells4,5,6,7. The recently developed homology-independent targeted integration (HITI) strategy allows for high-efficiency DNA knock-in in post-mitotic cells and enables effective knock-in of the fluorescent reporter in in vivo murine heart tissues at postnatal day 127. However, HITI replaces the targeted genome via a NHEJ-based mechanism, and thereby potentially causes insertion or deletion mutations at the junction site after successful knock-in. Furthermore, techniques for precise genome replacement in adult murine heart tissues have not yet been developed. In this report we demonstrated for the first time that AAV-mediated gene delivery promotes precise targeted integration in cardiomyocytes in in vivo murine adult heart tissues independently of DNA synthesis. In spite of the comparable transduction efficiency in cardiomyocytes, AdV-mediated gene delivery does not promote targeted integration in cardiomyocytes without S-phase entry. These data suggest that AAV-mediated gene delivery is the critical determinant for promoting targeted integration in non-dividing cardiomyocytes, consistent with previous findings that demonstrated AAV-mediated HDR in post-mitotic neuronal cells28.
AAV contains a transgene flanked by inverted terminal repeats, and the transgene is introduced into infected cells as a ssDNA18. Among the repair pathways after DNA double-strand breaks, the SSTR pathway using ssODN is highly effective in human cell lines29 and human hematopoietic stem cells30 . A recent report using CRISPR inhibition screening clarified that SSTR after genome editing required the FA pathway, and knockdown of the genes involved in this pathway, including FANCA, significantly decreased the efficiency of SSTR in K562 cells15. In our experiments, knockdown of Fanca significantly decreased the efficiency of targeted integration both in cultured cardiomyocytes and in vivo in the heart. FANCA promotes DNA double-strand break repair via single-strand annealing or strand exchange, and mutant FANCA protein with genetic variants of FA patients demonstrates defective biochemical activity in single-strand annealing or strand exchange31. Because FA pathway is generally considered to be a repair mechanism targeting interstrand crosslinks24, genomic structure formed during the repairing process in cardiomyocytes using ssDNA introduced by AAV as a transgene may resemble the conformation targeted by FA pathway. In our experimental conditions, knockdown of Brca2 decreased the efficiency of targeted integration in cultured cardiomyocytes. Although Fanca and Brca2 are ubiquitously expressed in various tissues, the decreased efficiency of AAV-mediated targeted integration in cardiomyocytes via knockdown of these genes suggests that SSTR-mediated DNA repair plays a pivotal role in terminally differentiated cardiomyocytes.
Introduction of the tdTomato sequence into the 3′-terminus of the Myl2 gene did not significantly injure cardiomyocytes in vitro 6 days after transduction of AAV69, or heart tissues in vivo 28 days after direct injection of AAV6. These data suggest that Mlc2v-tdTomato fusion protein produced by the recombined genetic locus did not significantly affect sarcomere function, and the Myl2 gene might be an appropriate target for real-time imaging of sarcomere structure even in the in vivo heart. A recent genome editing study using human hiPSCs demonstrated that fluorescent gene tagging of the genes involved in sarcomere function (TTN, MYL7, TNNI1, ACTN2, and MYL2) allowed live visualization of their proteins, which can potentially provide a useful research tool targeting cardiac morphogenesis13. Although these methods are useful for visualization and functional analysis of sarcomere proteins, the consecutive steps from genome editing to clonal selection of the edited hiPSCs is required. Furthermore, image-based validation of successful recombination must be confirmed after cardiomyocyte differentiation from hiPSCs, and is thereby time-consuming. Our method rapidly introduced the tdTomato sequence into the 3′-terminus of the MYL2 gene in differentiated contracting cardiomyocytes, and therefore this technique can potentially be used in various disease-specific hiPSC-CMs.
Rapid advances in high-throughput sequencing technologies have confirmed the high incidence of pathological genetic mutations in hereditary cardiomyopathies that lead to advanced heart failure32,33,34,35. CRISPR/Cas9 genome editing technologies are now generally recognized as potential tools for directly correcting genetic mutations6,36. HDR is a promising mechanism to correct pathogenic gene mutations, and is thereby expected to be a therapeutic tool for intractable cardiomyopathies. However, at this stage, the therapeutic application of targeted genome replacement in heart tissues is associated with several challenging problems that have yet to be overcome. First, our experiments indicate that the introduction of a high copy number of transgenes is required to promote targeted integration in the murine heart in vivo. Gene replacement therapy using AAV1 encoding SERCA2a (sarcoplasmic/endoplasmic reticulum Ca2+-ATPase) into the human heart via intracoronary administration failed to improve the clinical course of patients with heart failure37. The major reason for the negative results of the clinical study is presumed to be the low amount of vector DNA detected in these tissues, suggesting that it may be difficult to establish an efficient delivery system to transfer the target gene into heart tissues. Second, a recent study using a mouse model of Duchenne muscular dystrophy demonstrated that AAV-mediated genome editing therapy resulted in undesirable integration of AAV genomes at a higher rate than previously considered38. These data raise caution regarding the premature therapeutic application of genome editing, and indicate the need for careful consideration and further studies. Nevertheless, the data presented in the current study provide a foundation for the future development of genome engineering in non-dividing cardiomyocytes.
Materials and methods
Cell culture and animal procedures
HEK293T cells were maintained in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS, Gibco) and penicillin, streptomycin and glutamine (PSG, Gibco). Neonatal mouse cardiomyocytes were prepared as previously described with modification39. In brief, harvested hearts were incubated in 0.025% trypsin/EDTA (Sigma) at 4 °C overnight and then digested with collagenase type II (Worthington). The cardiomyocyte fraction was collected after differential plating for 70 min at 37 °C, seeded and incubated with high glucose DMEM with 10% fetal bovine serum and PSG. The adhering cardiac fibroblast were separately incubated and used for experimental analysis after more than one passage. For animal procedures, a combination anesthetic was prepared with 0.3 mg/kg of medetomidine, 4.0 mg/kg of midazolam, and 5.0 mg/kg of butorphanol. The anesthetics were administered to 8-week old male Cas9 knock-in mice by intraperitoneal injection, and left lateral thoracotomy was performed under mechanical ventilation. For direct injection, 10 μl of AAV solution was directly injected into the left ventricular myocardium. For orbital injections, 100 μl of AAV solution was injected into the orbital venous sinus of the left or right eye. All procedures were performed in conformity with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication, 8th Edition, 2011) and were approved by the Osaka University Committee for Laboratory Animal Use.
gRNA and repair template constructs
gRNA and repair template constructs constructs were generated as previously described9. DNA sequences for 5′-terminal homology arm (5′-HA) and 3′-terminal homology arm (3′-HA) were amplified from genomic DNA using the primer pairs including recognition sites of restriction enzymes. DNA sequences for tdTomato fluorescent proteins was amplified from pCSCMV:tdTomato vector (Addgene #30,530). Each DNA fragment was digested by restriction enzymes and cloned into pUC19 vector (TaKaRa) to generate repair template. hU6 promoter and downstream guide RNA (gRNA) sequence was amplified from pX459 vector (Addgene #48,139). hU6 promoter sequence, gRNA sequence and repair template sequence were cloned into between inverse terminal repeat (ITR) sequences in pAAV vector (TaKaRa). PAM sequence mutation was introduced into each repair template to avoid Cas9-mediated cleavage. gRNA and primer sequences are listed in Supplementary Table.
High-content image analysis
High-content image analysis was performed as previously described9,40. Briefly, cardiomyocytes (3 × 104 cells/well) or cardiac fibroblasts (5 × 103 cells/well) isolated from neonatal hearts of Cas9 knock-in mice were seeded in Greiner CELLSTAR 96-well plates precoated with collagen (Celmatrix Type I-C (KURABO)) one day before transduction with AAV or AdV encoding gRNA and repair template. Twenty-four h after primary culture, AAV or AdV encoding gRNA and repair template were transduced. Twelve h after transduction, medium were exchanged and cells were incubated for the indicated time. For sequential observation, fluorescent images from living cardiomyocytes cultured in 96-well plates were obtained by IN Cell Analyzer 6,000 (GE). After sequential observation, cells were fixed, immunostained and fluorescent images were obtained with the same imaging acquisition protocol. A total of 64 nonoverlap images (16 images per well) were obtained from each sample in one experiment using a 20x/0.45NA Nikon lens. The proportion of targeted integration was calculated as the ratio of tdTomato-positive cells per total cardiomyocytes using IN Cell Developer Toolbox (GE). The number of AdV-transduced cardiomyocytes in which tdTomato signals co-localized with sarcomeres was manually counted in all the acquired images because of these cells’ relatively low frequency.
EdU labeling and imaging analysis
EdU labeling and imaging analysis were performed as previously described9. Briefly, murine neonatal cardiomyocytes (3 × 104 cells/well) were seeded in Greiner CELLSTAR 96-well plates which were previously coated with collagen (Celmatrix Type I-C (KURABO)). Human iPSC-CMs (2 × 104 cells/well) were replated in 96-well plates previously coated with gelatin (Nitta Gelatin). EdU was added to the culture medium from a 100 mM stock in DMSO (final concentration: 10 μM). After EdU labeling for the indicated time, cells were washed with PBS and fixed. The labeled EdU of the cells were visualized by Click-iT imaging kits (Life Technology) according to the manufacturer’s instructions. The samples were incubated with the working solution of Click-iT reaction cocktail, containing the Alexa Fluor azide and CuSO4, for 30 min at room temperature. After removal of the reaction cocktail, cells were washed with Click-iT reaction rinse buffer and PBS, then transferred to immunostaining procedures. After incubation with blocking buffer (1% BSA in PBS), the cells were immunostained with the indicated antibodies. Nuclei were stained with Hoechst. The immunofluorescent images were obtained using IN Cell Analyzer 6,000. A total of 16 nonoverlap images per well were obtained using a 20x/0.45NA Nikon lens. The obtained images were analyzed using IN Cell Developer toolbox (GE) as previously described9. For in vivo labeling, mice were treated with EdU at a dosage of 5 mg/kg body weight through intraperitoneal injections 2 h before AAV injections and for 5 consecutive days. Six days after AAV injections mice were sacrificed and heart samples were collected. EdU detection was carried out before immunofluorescent staining using the Click-iT imaging kits (Life Technology).
Laser microdissection of heart tissue
Frozen sections of 10-μm thickness from heart cryoembedments were mounted onto RNase-free Polyethylene Naphthalate membrane frame slides (Leica 11,505,189). The sections were quickly fixed with cold acetone for 3 min, washed briefly in DEPC water, and stained with 0.05% toluidine blue solution for 1 min. Stained sections were then briefly rinsed 2 times in DEPC water and air dried for 10 min. tdTomato-positive cardiomyocytes were microscopically identified and isolated by laser microdissection at 20 × magnification using a LMD7000 Leica laser microdissection system. tdTomato-positive and -negative cardiomyocytes were pooled in 50 μl of QIAGEN RNeasy Micro Kit buffer dispensed centrally in the lid of a 0.5-ml Eppendorf cap. RNA was extracted according to the QIAGEN RNeasy protocol. Genomic DNA was extracted using a QIAAmp DNA micro kit (QIAGEN).
Generation and purification of AAV
Generation and purification of AAV were performed as previously described9. To generate AAV2 or AAV6, HEK293T cells were transfected with the vector of interest, pHelper vector and pRC2-mi342 or pRC6 Vector (AAVpro Helper Free System, TaKaRa) using calcium phosphate transfection (CalPhos Mammalian Transfection Kit,TaKaRa). To generate AAV9, pAAV2/9n plasmid (Addgene #112,865, a gift from James M. Wilson) was used for packaging. Seventy-two h after transfection, HEK293T cells were detached by addition of 1/80 volume of 0.5 M EDTA (pH 8.0), then pelleted via low-speed centrifugation (2000 × g for 10 min). Cell pellet was lysed with AAV Extraction Solution A and centrifuged (9,000 × g for 10 min). AAV Extraction Solution B was added to the collected to supernatant and stored at -80 °C. Collected AAV generated from HEK293T cells (8–9 × 107 cells) was purified using AAVpro Purification Kit (TaKaRa), and viral titer was calculated using AAV Titration Kit (TaKaRa) as previously described9.
Generation and purification of AdV
Adenoviruses were generated using the ViraPower Adenovirus Expression System (Invitrogen). The transgene between ITRs of the AAV vector was subcloned into the pENTR/D-TOPO vector using a pENTR Directional TOPO Cloning Kit (Invitrogen). The DNA inserts were transferred to the pAd/CMV/V5-DEST vector via LR reaction with a Gateway System (Invitrogen). The plasmids were digested with Pac I (New England BioLabs), purified and transfected into subconfluent 293A cells using Lipofectamine 3,000 (Invitrogen). Then 293A cells were cultured for 10–12 days with replacement of the medium every 2 days. The cells and culture medium were harvested, freeze-thawed twice, and centrifuged to obtain the adenovirus-enriched supernatants. To amplify adenoviruses, the aliquots of the supernatants were added to cultured 293A. After 2 – 4 amplifications, the adenovirus-containing media were purified using an Adeno-X Maxi Purification Kit (TaKaRa). Viral titers were determined by a plaque-forming assay using 293A cells according to the manufacturer’s instruction.
Human iPS cell generation and differentiation
The use of patient-derived samples was approved by the Ethics Committee of Osaka University hospital, and written informed consent was obtained and all research was performed in accordance with Ethical Guidelines for Medical and Health Research Involving Human Subjects. hiPSCs were generated from peripheral blood mononuclear cells (PBMCs) from a patient with cardiomyopathy. PBMCs were separated from peripheral whole blood using Ficoll-Plaque (GE). Reprogramming was performed by Sendai virus vectors with OCT3/4, SOX2, KLF4, and c-MYC (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Life Technologies). hiPSCs were maintained in medium (StemFit AK02, AJINOMOTO) on a laminin-coated plate. hiPSCs were differentiated into cardiomyocytes according to a chemically defined protocol and maintained in medium supplemented with human albumin and ascorbic acid25.
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This work was supported by JSPS KAKENHI Grant Numbers 18K08069, 18K19543, 19K08489, 19K16518, grants-in-aid from the Japanese Ministry of Health, Labor, and Welfare, the Japan agency for medical research and development (19bm0804008h0003). This work was also supported by Novartis Pharma, Takeda Pharmaceutical Company, Lilly, MSD, Daiichi Sankyo, Mitsubishi Tanabe Pharma, the Cell Science Research Foundation. This work was supported by the Grant for Basic Research of the Japanese Circulation Society (2018). Department of Medical Therapeutics for Heart Failure is an endowment department, supported by Actelion Pharmaceuticals Japan. We thank M. Moriyasu for technical assistance. This study was supported by Center of Medical Innovation and Translational Research, and Center for Medical Research and Education in Graduate School of Medicine, Osaka University.
The authors declare no competing interests.
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Kohama, Y., Higo, S., Masumura, Y. et al. Adeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes. Sci Rep 10, 15348 (2020). https://doi.org/10.1038/s41598-020-72216-y
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