Expression profiles of RsSEPGs during the inoculation of Rhizoctonia solani AG-1 IA on detached leaves of Brachypodium distachyon. (a to c) Expression of 9, 11, and 9 RsSEPGs of R. solani categorized as cluster 4 (a), 5 (b), and 6 (c), respectively, on B. distachyon at 6, 10, 16, 24, and 32 h post-inoculation with the improved method. Gene expressions were examined using qRT-PCR using cDNA prepared with total RNAs extracted from R. solani-inoculated B. distachyon leaves at each time point. Graphs display the relative mRNA level of each gene normalized by 18S rRNA as an internal control. The data are presented as means with the standard error of 4 biological replicates. Experiments were performed twice with similar results except for AG1IA_00431, AG1IA_08487, AG1IA_08650, and AG1IA_10146 marked with asterisks and a representative result is shown.