Evaluation of the inoculation method using Rhizoctonia solani AG-1 IA genes whose expression patterns during inoculation in rice plants have been characterized. (a and b) Expression patterns of 4 genes (AG1IA_08771, AG1IA_07787, AG1IA_06890, and AG1IA_06618) encoding carbohydrate-active enzymes (a) and 3 secretory effector-like protein genes (AG1IA_07795, AG1IA_09161, and AG1IA_05310) (b) of R. solani AG-1 IA on Brachypodium distachyon. Gene expressions were examined using qRT-PCR using cDNA prepared with total RNAs extracted from R. solani-inoculated B. distachyon leaves at 6, 10, 16, 24, and 32 hpi. Graphs display the relative mRNA level of each gene normalized by 18S rRNA as an internal control. The data are presented as means with the standard error of 4 biological replicates. Experiments were performed twice with similar results and a representative result is shown.