Mortality data and logistic fit (dose–response curve) for IFD with 532 nm, 25 ms pulse duration, and 2.5 mm spot size. Each data point represents the mortality outcome from a set of 40 subjects, and error bars represent 95% confidence intervals from the binomial distribution. The intersections of the dashed horizontal line at 90% mortality with the dose–response curves indicate the LD90 points, which are also presented in Table 2. IFD logistic fit (solid line) had pseudo R2 value of 0.99 as shown in Table 2. Dashed curve represents the fitted dose–response curve for the same laser conditions from previous anesthetized dosing work for comparison.