An examination of single day vs. multi-day heart rate variability and its relationship to heart rate recovery following maximal aerobic exercise in females


The purpose of this study was to examine the relationship of a single day measure of heart rate variability (HRV), and the averaged baseline measures of HRV to heart rate recovery (HRR) following maximal exercise. Thirty females (22.9 ± 3.2 years, 64.8 ± 8.4 kg) completed four visits (V1–V4), where a 10-min HRV was recorded. Upon completing the V4 recording, a treadmill graded exercise test (GXT) was performed, followed by a 5-min active cool down. HRV was assessed through time domain measures [natural log of root mean square of successive R–R differences (lnRMSSD) and standard deviation of normal to normal intervals (lnSDNN)] and natural log frequency domain measures [low frequency (lnLF) and high frequency (lnHF)]. Variables collected over V1–V4 were measured as; day of (DO) GXT, 3 day (AV3), and 4 day average (AV4). HRR was calculated as the maximal HR achieved minus the HR at: 30-s (HRR30), 1-min (HRR1), 2-min (HRR2), 3-min (HRR3), 4-min (HRR4) or 5-min (HRR5) of recovery. Pearson’s Product correlations revealed significant correlations (P = < 0.05) between all HRVDO measures to each HRR measure and are presented in ranges: lnSDNN (r = 0.442–0.522), lnRMSSD (r = 0.458–0.514), lnLF (r = 0.368–0.469), lnHF (r = 0.422–0.493). For HRVAV3, lnRMSSDAV3 and HRR1 were positively correlated (r = 0.390, P = 0.033). Last, HRVAV4 showed positive relationships (P = < 0.05) between lnRMSSDAV4 and HRR30 (r = 0.365, P = 0.048); and for HRR1 and lnSDNNAV4 (r = 0.400, P = 0.029), lnRMSSDAV4 (r = 0.442, P = 0.014), and lnHFAV4 (r = 0.368, P = 0.045); and lnRMSSDAV4 and HRR3 (r = 0.381, P = 0.038). Within the current study HRVDO displayed the strongest correlations to HRR therefore, averaged resting HRV measures do not strengthen the prediction of cardiovascular recovery following a GXT in this population.


The indirect evaluation of the autonomic nervous system (ANS) is performed through the assessment of cardiovascular metrics such as heart rate variability (HRV) and heart rate recovery (HRR)1,2,3. Heart rate variability quantifies the timing between consecutive beat-to-beat intervals and provides an indirect measure of systemic ANS activity2,4. In applied settings, resting HRV is used as an indicator of physiological readiness and recovery by estimating or predicting the ANS’s ability to respond to demands placed on the system2,5,6. More specifically, the higher the variability the greater the parasympathetic nervous system (PNS) influence (i.e., greater recovery status), whereas depressed HRV values are indicative of increased sympathetic nervous system (SNS) activity (i.e., lower recovery status)7,8. In addition to HRV, HRR is used to examine autonomic function and/or vagal tone reactivation following submaximal and maximal exercise1,4,9 in order to provide insight into ANS health. Heart rate recovery is commonly calculated as the change in peak heart rate (HR) achieved during submaximal or maximal exercise from HR at specific time points during recovery (e.g. 30 s, 1-min, etc.)4. Segmental measurements of HRR can be broken down into two separate phases, a fast phase and a slow phase. The fast phase (30 s to 2-min) represents PNS reactivation whereas the slow phase (2- to 5-min) is suggestive of the combined activity of PNS reactivation and SNS withdrawal4,9,10.

Together, HRV and HRR provide wider insight to cardiac autonomic regulation which can then translate to clinical or applied applications. The shared system of regulation between HRV and HRR suggest that the two measures may be interrelated. If such a relationship exists, resting HRV indices may be used as a predictor of HRR (ANS rebound) prior to a stressful stimulation such as exercise and potentially reduce the risk of clinical testing or reduce periods of overreaching in athletic populations. Thus, the relationship between resting HRV to post exercise HRR has shown to be a promising area of evaluation; however equivocal results have been found11,12,13,14,15,16. The apparent disconnect within the literature between resting HRV and HRR may be explained through procedural differences, study population, analysis techniques, or exercise and recovery protocols4. Further investigations should be performed to aid in the deduction of the various findings. Although there are numerous possibilities that may contribute to the conflicting findings within the literature, the use of a single resting measure of HRV showed to be a commonality between them. It is important to note that HRV, by nature, is a highly sensitive measure to factors such as quality of sleep, stress, and the environment which may lead to a change in day-to-day fluctuations within HRV indices. With these known influencers of HRV, a single day HRV measure may not provide a comprehensive representation of ANS status5,17. In order to account for potential day-to-day fluctuations, the use of multiple baseline values of HRV have been proposed and utilized in other areas of HRV research to provide a more precise estimate of autonomic function within an individual or population5,18.

The majority of research evaluating the use of averaged HRV indices over a series of days is primarily focused on guided training within athletic populations5,18,19,20. Recent studies by Plews et al., found that averaged resting HRV values over several days to be a better predictor of recovery status and tool to guide training versus a single day measure19,20,21,22. As the research advances with the use of averaged HRV indices and guided training, it becomes evident that these approaches may carry over to other physiological applications such as evaluating acute stress and recovery (e.g., exercise). To the authors’ knowledge, only one study has examined a potential relationship between averaged HRV baseline measures and HRR16. Tonello et al. examined 21 apparently healthy overweight women (34.5 ± 6.4 years of age) where HRV was collected for four consecutive days in a seated and standing position. Heart rate recovery was also collected over four consecutive days and analyzed at 1, 2, 3, and 5 min following submaximal exercise. Despite the use of averaged HRV measurements, no significant correlations were observed between HRR and HRV16. Although Tonello et al.16 had a novel approach to examine day-to-day HRV and HRR, it is currently unknown the relationship between averaged HRV measures on HRR following a single exercise bout within young healthy females. Therefore, the purpose of this study was to examine the relationships between a single day, a 3-day average, and a 4-day average baseline of HRV and HR measurements to HRR following a maximal aerobic exercise test in young healthy females.



Prior to the collection of any data, the Kennesaw State University Institutional Review Board approved all testing procedures and protocols, and all experiments were performed in accordance with relevant guidelines and regulations. A convenience sample of thirty-four apparently healthy, young apparently healthy females (22 ± 3 years of age, 64.9 ± 8.4 kg, 161.3 ± 6.6 cm) were recruited from the local metropolitan area. Each individual was made aware of the procedures and potential risks associated with the study and signed an informed consent prior to participation. Inclusion criteria required participants to be classified as “low risk” for exercise participation as defined by the guidelines of the American College of Sport Medicine23. Participants also filled out a health history questionnaire and any individual who reported having orthopedic conditions, or cardiovascular, pulmonary, or metabolic disease was excluded from the study. Prior to all sessions, participants were asked to wear clothing suited for exercise that was light and comfortable, fast for a minimum of 4 h (except for water consumption), avoid exercise and alcohol for 24-h, and avoid caffeine consumption for 12-h.

Experimental design

Participants reported to the institution’s exercise physiology laboratory on four separate occasions where the first three visits occurred within a 7 day period of each other and the fourth visit occurred within 7 days of the third visit. All visits were conducted between the hours of 4 a.m.–9 a.m. and were performed as close to wake time as possible. Visits one (V1), two (V2), and three (V3) were designed to collect resting cardiac autonomic measures, whereas visit four (V4) was used to collect cardiovascular measures prior to and following a maximal GXT. Upon arrival to V1–V3, baseline resting cardiac autonomic measures were collected using an advanced heart rate monitor for 10-min in the supine position. At the completion of the resting period, the visit ended. These procedures held true for V1–V3 with the exception of V1, where anthropometric measures were collected. Upon arrival to V4, resting cardiovascular measures were collected using the same procedures as V1–V3. At the completion of the resting period, participants performed a maximal GXT on a motorized treadmill. Immediately after the GXT participants began a standardized 5-min active cool down (see Fig. 1).

Figure 1

Study design. HRV heart rate variability, GXT maximal graded exercise test.

Cardiac autonomic measures

Heart rate and HRV were recorded with the Polar Team2 system (Polar, Lake Success, NY). Each heart rate monitor was fitted to the participant and positioned directly over the sternum and was in direct contact with skin. All resting cardiac autonomic measures were recorded in a quiet, dimly lit room at an average temperature of 21.6 ± 0.9 °C and 53.6 ± 10.7% humidity. Each resting period took place in the supine position for 10-min and participants were instructed to breathe at their normal respiratory rate and to remain as still as possible until further instructed.

Heart rate variability and resting heart rate (RHR) were analyzed in 5-min segments, where the first 5-min of the recordings were discarded in order to allow for an adjustment period. Thus, only the last 5-min of the resting period were analyzed for HRV and RHR. Measurements of HRV were represented and analyzed as a single day measurement collected on the day of the maximal exercise test on V4 (DO), the average of V1–V3 (AV3), and the average of V1–V4 (AV4), respectively. Time and frequency domain measures were analyzed using Kubios software version 3.0 (Kubios V 3.0, Joensuu Finland) and set to an interpolation rate of 4 Hz. In order to detect the presence of artifact or noise, Kubios software used the piecewise cubic spline interpolation method to filter the data with a “very low-low artifact correction” and a sensitivity set to identify any R–R abnormalities ± 0.35 s compared to the local average24. Segments were visually inspected by a trained member of the research team for the presence of irregular R–R intervals (i.e. ectopic beats) and if the presence of three or more irregular R–R intervals were included in a segment, that segment was deleted in order to avoid misinterpretation of analysis.

Time domain measures of HRV chosen for the current study included the root mean square of successive R–R differences (RMSSD) as an indication of PNS activity25 and the standard deviation of normal to normal intervals (SDNN)2. Time domain analysis occurred through the process of converting R–R intervals into a tachogram where the y-axis represents the time (ms) between consecutive intervals and the x-axis represents the number of beats over time26. In addition to the time domain measures, the frequency domain measures chosen for the current study included low frequency (LF) and high frequency (HF). Frequency domain measures were extrapolated through the Fast Fourier Transformation (FFT) algorithm with the window width set at 300 s and a 50% overlap. The algorithm identified LF as signals between 0.04 and 0.14 Hz and signals from 0.15 to 0.4 Hz as HF. Low frequency is established as a universal measure that indicates both PNS and SNS activity, whereas HF represents PNS activity26.

Graded exercise test and active cool down

At the completion of the resting period during V4, participants were allotted 5-min to warm-up on a motorized Woodway treadmill (Woodway USA, Waukesha, WI) at a self-selected pace. Following the warm-up, participants were fitted to a Parvomedics Metabolic Cart (Parvomedics TrueOne 2400, Sandy, UT) in order to assess peak aerobic capacity (VO2peak). The GXT was performed at a self-selected pace where the grade of the treadmill increased 1% each minute of the test until the participant reached volitional fatigue. Blood lactate was assessed immediately upon completion of the GXT via finger stick and portable lactate analyzer (Lactate Plus, Nova Biomedical, Waltham, MA). Simultaneously, the treadmill was set at a walking speed of 1.5 mph and 2.5% grade1 and participants walked for 5 min in order to cool down. Heart rate recovery measures were collected during the cool down.

Heart rate recovery

Heart rate recovery was assessed through the Polar Team2 system software and calculated as the maximal heart rate achieved during the test minus the recovering HR at 30 s (HRR30), 1 min (HRR1), 2 min (HRR2), 3 min (HRR3), 4 min (HRR4), and 5 min (HRR5) of recovery. These measures incorporated both phases of HRR, which include a fast phase (30 s to 2-min) that represents PNS reactivation and the slow phase (2–5 min) which represents a combination of PNS and SNS activity9,10.

Statistical analysis

All data were entered and analyzed in SPSS 25 software (Chicago, Illinois, USA). Measurements of RMSSD (ms), SDNN (ms), LF (ms2), HF (ms2), and RHR (beats/min) were analyzed as a single day measurement (DO), the average of V1–V3 (AV3), and the average of V1–V4 (AV4). Prior to statistical analysis, all HRV measures were log transformed (ln) due to a violation in normality according to a Shapiro–Wilk Normality Test. Differences between DO, AV3, and AV4 within each HRV and RHR measure, respectfully, was examined through an analysis of variance (ANOVA) with a least square difference (LSD) post hoc applied in order to observe pairwise comparisons. Pearson product correlations were used to assess relationships between RHR measures (RHRDO, RHRAV3, RHRAV4), HRV measures as represented in the time domain (lnRMSSDDO, lnRMSSDAV3, lnRMSSDAV4, lnSDNNDO, lnSDNNAV3, lnSDNNAV4) and frequency domain measure (lnLFDO, lnLFAV3, lnLFAV4, lnHFDO, lnHFAV3, lnHFAV4) to the respected HRR measures; HRR30, HRR1, HRR2 HRR3, HRR4 and HRR5. Significance was set at ≤ 0.05. Results are presented as means and standard deviation (M ± SD).


A total of thirty apparently healthy females (22.9 ± 3.2 years, 161.3 ± 6.5 cm, 64.8 ± 8.4 kg, maximal aerobic capacity = 37.6 ± 6.6 ml/kg/min) completed the study. Four participants withdrew due to scheduling conflicts. Means and standard deviations of lnRMSSD, lnSDNN, lnLF, lnHF, RHR, HRR, as individual days and averaged visits are presented in Table 1. Pairwise comparisons revealed no significant differences between DO, AV3, AV4 measures of HRV (P > 0.05), however there were significant differences between DO, AV3, AV4 measures of RHR (P < 0.05). Pearson correlation values and significance are displayed in Table 2. Resting heart rate measured as DO, AV3, and AV4 were significantly negatively correlated to each time point of HRR (Table 2). Additionally, significant positive correlations were observed between all HRVDO measures to HRR30, HRR1, HRR2, HRR3, HRR4, HRR5; lnSDNN (r = 0.442–0.522), lnRMSSD (r = 0.458–0.514), lnLF (r = 0.368–0.469), lnHF (r = 0.422–0.493), respectively (Table 2). A single significant positive correlation was seen in HRVAV3 between lnRMSSDAV3 and HRR1 (r = 0.390, P = 0.033,). Last, HRVAV4 showed significant positive relationships between lnRMSSDAV4 and HRR30; lnSDNNAV4, lnRMSSDAV4, lnHFAV4 and HRR1; and lnRMSSDAV4 and HRR3 (Table 2).

Table 1 Resting raw and log transformed measures of HRV.
Table 2 Pearson product correlation values of resting HRV measures represented as a single day and averaged measure to HRR time points.


The purpose of this study was to examine the relationships between various baseline measures of HRV (DO, AV3, AV4) and HRR (30, 1, 2, 3, 4, 5) in young adult females following a maximal GXT. The authors hypothesized that HRVAV4 would result in a more robust significant positive relationship to measures of HRR than those measured as HRVDO or HRVAV3. The primary findings of the study do not support this hypothesis, indicating HRVDO possessed the strongest positive relationships with all HRR measures. Measures examined as HRVAV3 had the least amount of significant correlations with a single point of significance between lnRMSSDAV3 and HRR1. Compared to HRVAV3, more robust and varied relationships were observed between HRVAV4 and HRR measures, but remained weaker than those of HRVDO (Table 2).

Despite common physiological factors between resting HRV and HRR, the complexity behind their relationship and influence upon each other are not fully understood3,4,9,12,13,14,15,27,28. Esco et al. examined this relationship and found no correlations in time (SDNN) or frequency domain measures (HFnu, LFnu:HFnu) of resting HRV to HRR (1,2) following a similar GXT and recovery protocol in 66 healthy males12. These findings align with Bosquet et al. who observed a lack of significant correlations between resting HRV measures and HRR following a GXT on a treadmill in well-trained athletes29. Javorka et al. also examined the relationship between standing HRV indices and HRR1 in 17 healthy males following an 8-min step test and saw no correlations between the two different cardiac autonomic measures13. The current investigation does not fully support these observations, as significant correlations between resting HRV (DO, AV3, AV4) and HRR were reported. This may in part be due to the differences in population observed; where in the current study recreationally active females participated compared to either males or athletic populations in the aforementioned studies. The significant correlations between HRVDO and HRR in the current study agree with those of Nunan et al., who found moderate and positive relationships between parasympathetic measures of lnRMSSD to HRR (2,3), lnHF to HRR (1,2,3), and moderate negative correlations between lnLF:HF to HRR (1,2,3) following a GXT in both male and female subjects15. Additionally, a recent study by Molina et al.14, observed moderate negative correlations between resting PNS measures and moderate positive correlations in measures of combined PNS and SNS indices to HRR (3,5)14. The systems involved in regulating ANS function at rest and in response to exercise are complex and highly interactive resulting in a variety of measures used to interpret the various components of this system. Therefore, interpretations should take into consideration external and intrinsic factors (e.g., sex, body comp, training status, anxiety…) when comparing these markers.

The current study demonstrated a positive relationship between resting HRV and HRR which is equivocal to current literature11,12,13,16,27. Though it was out of the scope of this study to examine mechanisms related to the differences in HRV and HRR measures, other factors such as resting cardiovascular values, methodological approaches, and homogeneity of population may explain the differences found within the literature4,12,14,16. The driving theory behind the hypothesis of the current investigation was the possible limitation of using single day resting HRV measures as a baseline. Several investigations have revealed significant day-to-day fluctuations in HRV, suggesting that a single day measurement may not provide a precise representation of resting autonomic indices5,19,20,21,30. Research by Plews et al.20 found that rolling averages22 may provide more insightful information to a resting state with a 7-day rolling average as the accepted standard. Despite these recent findings, the use of single day HRV measurements prior to an exercise intervention or acute study is common practice31,32,33. To the authors’ knowledge, only one other study examined the relationship between averaged HRV measures and HRR16. Tonello et al.16 examined the relationship of a 4-day average resting HRV to HRR following a maximal exercise test in 21 apparently healthy overweight women (34.5 ± 6.4 years of age). In addition to a 4-day assessment of HRV, HRR was also assessed over 4 days following a submaximal cycle ergometer test. Interestingly, no correlations were observed between resting HRVAV4 and HRR16. The primary outcome variables of the study conducted by Tonello et al.16 varied from the current study and therefore the differences to the current investigation may be due to study design (i.e. submaximal cycle test vs treadmill GXT)4 and population.

The findings of the current study differ from Tonello et al.16 as significant relationships were observed between averaged HRV measures and HRR. However, while interpreting the relationships found between HRVAV4 and HRR (30,1,2,3), the strength of these relationships were consistently weaker in averaged markers compared to HRVDO (see Table 2). Specifically, for HRVAV3 the HRVDO was not included in the average and demonstrated the fewest relationships. When considering HRVAV4, the relationships improved considerably, but are still less than that of HRVDO, leading the investigators to believe that this may be artifact driven by the strength of the HRVDO measure and should be considered when interpreting the relationships found in the HRV averaged measures. This is in direct contrast to the hypothesis of this study as well as several prior studies utilizing single day measurements12,13,29. A potential explanation for this is pre-exercise anticipation, which has been shown to negatively alter HRV during resting periods34. When observing average HRV over the four measurements, the DO had the lowest values (Table 1), demonstrating this effect. Since this was a single bout of exercise, it cannot be determined if this had a deleterious effect on exercise performance.

When evaluating the various indices of HRV and HRR there are a wide array of populations and protocols utilized within the literature, and as such, there becomes a staggering number of variants that could be accounted for. This study was the first step towards a better understanding of the application of average HRV on ANS recovery, and was not an attempt to control for all of these variations found within the literature, and therefore came with inherent limitations. First, the participant population was a relatively homogenous sample of young, healthy females. Therefore, results cannot be applied to older adults, unhealthy populations, or males. Relationships between sex and race were not evaluated, and future studies should include both males and females. Additionally, future studies should include HRV measures collected over consecutive and nonconsecutive days, whereas our study is limited to the evaluation of HRV measures between nonconsecutive days.


Resting heart rate measures displayed the most robust correlation to HRR values. Regarding HRV measures and HRR, HRVDO demonstrated the strongest relationship to HRR and is likely the primary influencing factor of the observed correlations between HRR and HRVAV4. Therefore, the authors conclude that averaged 3–4 day HRV measures do not strengthen the prediction of cardiovascular recovery following a GXT within the confines of this study. Though mechanisms were not identified, these findings provide new and interesting insight into the relationship of HRR and resting HRV. With the continued application of HRV as tool for guided interventions, the understanding of the ANS and its relationship to recovery is paramount. The findings of this study are in line with those who found significant correlations between resting HRV values and HRR following a maximal exercise test, however the use of averaged (non-consecutive) measures of HRV may not provide further insight to aid in the explanation of equivocal findings throughout the literature11,12,13,14,15,16,27,29. Future research should include clinical populations due to differences in resting and stress related physiology. In addition, other physiological measures such as biomarkers of stress and metabolism to better reflect resting status, which will provide more insight into the system as a whole.



Autonomic nervous system


Heart rate variability averaged over the first three visits


Heart rate variability averaged over all four visits


Heart rate variability measured the day of the graded exercise test (visit 4)


Graded Exercise Test


Heart Rate Recovery


Heart Rate Recovery 30 s following graded exercise test


Heart Rate Recovery 1 min following graded exercise test


Heart Rate Recovery 2 min following graded exercise test


Heart Rate Recovery 3 min following graded exercise test


Heart Rate Recovery 4 min following graded exercise test


Heart Rate Recovery 5 min following graded exercise test


High frequency


Heart rate variability


Low frequency


Natural log


Parasympathetic nervous system


Root mean squared of successive R–R intervals


Standard deviation of normal to normal intervals


Sympathetic nervous system


First visit to the exercise physiology laboratory


Second visit to the exercise physiology laboratory


Third visit to the exercise physiology laboratory


Fourth visit to the exercise physiology laboratory

VO2peak :

Peak oxygen uptake


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E.B. wrote the manuscript, analyzed data, designed research, and conducted experiments. B.K. and C.M. made significant contributions to the manuscript, research design, and analysis. M.T. and M.E. assisted in the statistical analysis and research design. R.L. had significant contributions to conduct experiments.

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Correspondence to Brian Kliszczewicz.

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Bechke, E., Kliszczewicz, B., McLester, C. et al. An examination of single day vs. multi-day heart rate variability and its relationship to heart rate recovery following maximal aerobic exercise in females. Sci Rep 10, 14760 (2020).

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