Expression of the proliferation marker, Ki67, in spheroids from MG-63 cells on the parylene N-coated plate. (a) MG-63 cells (1 × 105 cells/ml) were cultured on parylene N-coated glass coverslips. After incubation for 12 h, 24 h, 48 h, and 72 h, and fixation, immunofluorescence images of Ki67 were obtained. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar: 50 μm. Experiments were performed in triplicate and repeated three times with similar results. (b) MG-63 cells (1 × 105 cells/ml) were seeded on parylene N-coated and control plates. After 72 h, Mki67 and PCNA mRNA levels in MG-63 cells on parylene N-coated and control plates measured by the quantitative real-time polymerase chain reaction. The expression levels of target genes were normalized to that of the housekeeping gene, B2M, using the 2−ΔΔCt method. Data represent the mean of three independent experiments ± SD. Experiments were performed in triplicate and repeated three times with similar results. *P < 0.05; **P < 0.01.