Figure 4 | Scientific Reports

Figure 4

From: Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells

Figure 4

Upregulation of differentiation markers in spheroids from MG-63 cells on parylene N film. (a) MG-63 cells (1 × 105 cells/ml) were seeded on parylene N-coated and control plates. After 72 h, ALPL, RUNX2, and BGLAP mRNA expression in MG-63 cells on parylene N-coated and control plates measured by the quantitative real-time polymerase chain reaction. Expression levels of the target genes were normalized to that of the housekeeping gene, B2M, using the 2−ΔΔCt method. Data represent the mean of three independent experiments ± SD. Experiments were performed in triplicate and repeated three times with similar results. (b) Alizarin Red S staining of MG-63 cells (1 × 105 cells/ml) grown on parylene N-coated and control plates for indicated incubation time. Scale bar: 100 μm. Experiments were performed in triplicate and repeated three times with similar results. (c) Quantification of Alizarin Red S was measured using MG-63 cells grown on parylene N-coated and control plates for 72 h incubation time. Data represent the mean of 6 replication ± SD. *P < 0.05; **P < 0.01.

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