Fabrication of multifunctional TANI/Cu2O/Ag nanocomposite for environmental abatement

During past decade, the ternary nanocomposite is ubiquitous in nanotechnology. Herein, a simple fabrication of cuprous oxide (Cu2O) and silver (Ag) nanoparticles into Tetraaniline (TANI) matrix by in situ-polymerization approach to attain Tetramer-Metal Oxide-Metal (TANI/Cu2O/Ag, shortly TCA) ternary composite was reported firstly. The synthesized materials were further characterized by a series of instrumental techniques to understand its structure, morphology and thermal properties. This nanocomposite showed promising applications in wastewater treatment by the testing of photocatalytic activity over the pararosaniline hydrochloride (PRA) dye degradation under visible light radiations, removal of Cadmium ion (Cd2+) by adsorption, corrosion resistance and antibacterial activity against both gram positive and gram negative bacterial strains. The obtained results of TCA compared with the pure TANI and binary nanocomposite (TANI/Cu2O) declared that the TCA composite is excellent material to solve the environmental issues due to lesser bandgap energy, visible light respond, high absorptivity, and long-life excitation.


Results and discussion
formation mechanism of tAni/cu 2 O/Ag NC. Initially, the monomer NPPD undergoes protonation by react with HNO 3 to obtain the protonated ions in toluene medium and then the protonated ions undergo polymerization in presence of aqueous solution of APS. Here, the interjunction is formed between the toluene and aqueous media. The ions maces together and extend themselves into straight chain (1, 4 joining of phenyl groups) but the side products didn't occur due to the fast reaction of anilinium ions. The protonation occurs on imine unit of quninonoid part of EB (TANI) and removes lone pair of electrons on nitrogen so that gets positive charge. The free NO 3 − accumulates onto the EB (green colour) because of electrostatic attraction between NO 3 − and TANI. The copper ions (Cu + ) of Cu 2 O and Ag + ions from AgNO 3 which accommodate onto TANI as presented in Fig. 1.
Characterisation study of prepared samples. XRD study. The pure phase formation of TANI, TANI/ Cu 2 O and TCA composite was confirmed by powder XRD and the diffraction peaks of prepared materials are presented in Fig. 2. The broad peak at 2θ = 25° confirmed the presence of TANI 29 along with other two major peaks at 20.6° and 27.5° due to the periodicity of parallel and perpendicular to the phenyl ring of TANI which authorizes the (110) plane and the peak was presented at 14.6° declared that formed TANI is amorphous in nature, thus XRD peaks are not sharp. Further, TANI implies four peaks have been observed in TCA NC with 2θ values of 38.4°, 44.9°, 65°and 78° which are consistent to their respective planes (111), (200), (220) and (311). The presence of sharp peak at 39°confirmed that Ag NPs are embedded in TANI/Cu 2 O/Ag matrix. The XRD www.nature.com/scientificreports/ pattern of Cu 2 O NPs has sharp peak at 2θ = 44° which corresponds to the (111) planes (standard JCPDS file No. 05-661) along with certain other peaks of low intensity 30 . However, these peaks show slightly shifted from their respective standard positions which may be due to the presence of Cu 2 O and Ag into TANI matrix. These results are further compiled with FTIR.
FTIR analysis. The FTIR spectra of synthesized Cu 2 O, TANI, TANI/Cu 2 O and TCA NCs are depicted in Fig. 3. The broad absorption peak in the range of 750-550 cm −1 can be attributed to C-NO 3 − bonding is observed in TANI. The sharp peak appeared at 834 cm −1 due to N-H bending vibrations. Two peaks were observed at 1,163 cm −1 and 1,307 cm −1 could be assigned to the presence of quinonoid ring (N = Q = N) and C-N stretching mode vibrations respectively. The peaks were centred at 1581 cm −1 and 1,489 cm −1 are attributed to C-N and C-C stretching mode for quinonoid and benzenoid rings 31 . The intensities of these two peaks are almost confirms that the prepared TANI is conductive with emeraldine base (EB) form. Another peak was presented at 2,919 cm −1 is due to the C-H stretching vibrations whereas the peak at 3,382 cm −1 for the N-H stretching vibrations. These peaks were slightly shifted from their normal positions of pure TANI 32 owing to the existence of Cu 2 O, Ag into TANI matrix.
FESEM-EDS analysis. The morphology and structural elemental composition of prepared Cu 2 O, TANI, TANI/Cu 2 O and TCA NCs were investigated by FESEM and EDS respectively. Figure 4a,b shows the FESEM   www.nature.com/scientificreports/ The presence of elements such as copper (Cu), oxygen (O), Silver (Ag), Carbon (C) and Nitrogen (N) in the EDS spectrum was confirmed the formation of pure TCA via a simple, facile insitu polymerization method ( Fig  S1).
Transmission emission microscopy (TEM). Further morphology of prepared TCA NC was studied using HRTEM and Selected area electron diffraction (SAED); the images ( Fig. 5a,b) indicated a poor crystalline nature of NC, supported by XRD results. It is in well agreement with the diffraction planes of TCA NC. The average particle size of TCA NC was 8.26 nm calculated from histogram ( Fig. 5c) using imageJ software.
X-ray photoelectron spectroscopy (XPS). Figure 6 displayed the electron spectroscopy of synthesized TCA NC was examined by XPS analysis. A polymer TANI contains the carbon and nitrogen shown the respective peaks of phenyl C1s, C-C (Fig. 6b) and nitrogen containing aromatic polymers N1s (Fig. 6c) were centred at 284.8 and 399.7 eV. Besides that, the Cu and O in Cu 2 O were observed at binding energy of 934.6 eV (Fig. 6d) for Cu 2p 3/2 , 955.02 eV for Cu 2p 1/2 and 532.2 eV (Fig. 6e) respectively 33 . When compared to Ag°, a slight peak lower shift was observed in prepared Ag which is located at 367 and 372 eV are ascribed to Ag3d 5/2 and Ag3d 3/2 respectively, this may be due to disturb the chemical environment around Ag NPs in synthesized composite 34 .
UV-visible/DRS spectroscopy. The UV-Visible absorption spectra of Cu 2 O, TANI, TANI/Cu 2 O and TCA NCs are shown in Fig. 7a. The UV-Visible absorption spectra of TANI, TANI/Cu 2 O and TCA NCs show a peak at 326 nm which corresponds to π-π* transition of benzenoid ring of TANI 35 . Figure 7b (a-c) shows the optical bandgap energies of TANI, TANI/Cu 2 O and TCA NCs obtained from the Eq. (1).
(1) E g = hν = hc/ = 1240/ www.nature.com/scientificreports/ where 'E g ' represents bandgap/forbidden energy, 'h' is plank's constant, 'ʋ' is wave frequency, 'c' is the speed of light and 'λ' is the wavelength. Table S1 in ESI clearly stated that the somewhat decrease in bandgap energy of TCA NC (E g = 2.82 eV) from pure TANI (E g = 2.93 eV) due to embedded the p-type semiconductor, Cu 2 O (E g = 1.98 eV) into TANI matrix. The wider bandgap value originates from the quantum confinement effect which is caused by the nanodimensional state of materials. Lesser the bandgap materials possess excellence in catalytic activity, thus TCA NC has potent applications than other materials prepared.
Thermogravimetric (TG-DTA) analysis. Thermogram of the prepared NCs in an argon atmosphere as a carrier at a flow rate of 10 °C/min with a temperature range of 30-1,000 °C is depicted in Fig. 8a. The resultant graph implies the change in mass of the sample with increase in temperature. It was observed that pure TCA NC undergo three major steps of weight loss patterns but TANI and TANI/Cu 2 O shows two major steps.  www.nature.com/scientificreports/ 1. The first small fraction of weight loss in the temperature range from 30-270 °C is due to the loss of hydrate molecules from the surface of TANI and TCA NC held through weak ionic interactions 36 . 2. The second major step of continuous weight loss occurs due to vanish the oligomers in TANI, TANI/Cu 2 O and TCA NCs at the temperature range of 270-540°C 37 . 3. Third step is decomposition process, shows a gradual weight loss occurs in the temperature range from 600-850 °C was observed due to thermal oxidative decomposition and degradation of the polymer fraction of TCA NC 38 . From the graph, it was seen that the thermal stability of TCA NC is considerably less than that of pure TANI and TANI/Cu 2 O NC which shows high thermal stability. This may be due to decreased in chain length and the presence of bulky groups in the composites. This thermal stability was decreased in TCA NC is due to addition of Ag, may cause a decrease in the tension induced by thermal excitation in the C-C bond 39 . Figure 8b shows the DTA curves of pure TANI, TANI/Cu 2 O and TCA NCs. This depict explored about thermal behaviour of composites from pure TANI. In Fig. 8b(C), the two exothermic peaks were observed at 280 and 420 °C, corresponding to their respective temperatures of polymer degradation and oxidation of NC. The oxidation at higher temperature means increase the content of Cu 2 O in TCA composite and is evident from the Thermogram ( Fig. 8a(C)). Figure 8b(B) shows the broad endothermic peak around the temperature of 440 °C, which corresponds to the decrease in degradation temperature of TANI/Cu 2 O. Figure 8b(A) confirms the presence of exothermic peak at the temperature of 240 °C, corresponding to decomposed the TANI. These results tells about the interaction of Cu 2 O and Ag NPs with polymers surfaces lead to formation of new compound of polymers which do not decompose into simple molecules such as carbon dioxide. The decrease in the degradation temperature of polymer nanocomposite to 280 °C, as compared to 240 °C of pure TANI, indicate that the polymers in the nanocomposite is different in its chemical nature compared to that in the pure state. However, the interpretation of the result needs further investigation.
Photoluminescence (PL) study. The photoluminescence spectra used not only to characterize the defects of a semiconductor but also to study the exciton spectra in TANI and its nanocomposites. Figure 9 illustrates the emission spectra of TANI and TCA NC at the excitation wavelength of 280 nm. In order to evidence the contribution of pure TANI and TCA NC shifted to 466 nm due to transitions from the polaronic band to the -band (HOMO) structures of TANI and the emission wavelength is shifted towards the blue when compared to the bulk TANI. This emission is due to the recombination of electron and hole pairs. Catalytic applications of prepared nanomaterials. Photocatalytic activity. The photocatalytic activities of TANI, Cu 2 O, TANI/Cu 2 O and TCA NCs were studied by selective organic pollutant pararosaniline (PRA) dye under visible light irradiation at normalized conditions such as dye concentration (10 mg/L), pH (11) and catalyst dose (30 mg). The characteristic absorption of PRA dye solution at the wavelength (λ = 545 nm) used to monitor the photocatalytic degradation process. Figure 10a-d shows the UV-Vis absorption spectra of PRA dye solutions by TANI, Cu 2 O, TANI/Cu 2 O and TCA composites for 90 min. These results declared that the deep green colour of the initial PRA solution gradually fades to colourless during the process of photodegradation as the exposure time is extended. Figure 10a shows the photocatalytic activity of TANI, clears that no significant photodegradation (11%) of PRA dye. Figure 10b shows the photocatalytic activity of Cu 2 O, stated that the dye was degraded partially (59%) due to faster recombination rate of electron and hole pair. Therefore, further, the binary composite TANI/ Cu 2 O was tested under same conditions and shows (Fig. 10c) the excellent photocatalytic activity (85%). So as to complete photodegradation of PRA dye, both Cu 2 O and Ag were fabricated onto   www.nature.com/scientificreports/ In addition, the rate order kinetics of prepared composites calculated using Eq. (2), according to the Langmuir-Hinshelwood kinetics model, the function of ln(C/C 0 ) versus reaction time (t) displayed a linear relationship as show in ESI (Fig S2b).
where 'C/C 0 ′ is normalized initial concentration, 't' is the reaction time, and 'k' is the reaction rate constant (min −1 ). The corresponding pseudo first order kinetic rate constant (k) and regression coefficient (R 2 ) of PRA were calculated and are listed in the Table S2 (ESI). These results confirmed that all are follows the pseudo first order rate.
Proposed mechanism. Proposed a plausible mechanism for improved the photocatalytic degradation efficiency of TANI/Cu 2 O/Ag ternary nanocomposite in the presence of visible light irradiation was displayed in schematic diagram Fig. 11. Initially, TANI was kept in visible light illumination, π → π * transition takes place, which release the electrons Eq. (5). In similar way, Cu 2 O undergoes charge separation process that leads to the promotion of electrons from valence band (VB) to conduction band (CB) and leaving a hole in the VB and releasing of electrons Eqs. (6,7) due to the formation of heterojunction. Ag reduced and resulting in release of electrons Eq. (8). Finally, the photogenerated electrons are captured by the TANI through semiconductor Cu 2 O due to heterojunction which helps in enhancing the photogenerated charge separation efficiency in ternary nanocomposite. Simultaneously an equal amount of holes have been formed in semiconductor nanocomposite. These separated electrons and holes directly reacts with oxygen and water to generate highly reactive superoxide ( ⋅ O 2 − ) and hydroxyl ( ⋅ OH) radicals Eq. (9). These energetic reactive radicals react with surface adsorbed PRA molecules and degraded them into CO 2 and H 2 O Eq. (10).
Based on experimental conditions, TCA ternary nanocomposite possesses high photocatalytic activity towards the degradation of PRA dye solution under visible light irradiation than the binary composite and pure TANI.

Radical affirmation by Scavenger test.
In order to endorse the radicals scavenging, initially the 10 mg/L PRA dye solution was taken along with 30 mg TCA NC allowed to irradiation for 70 min in the presence of scavengers such as tertiary butyl alcohol (t-BuOH), Benzoquinone (BQ) for OH, O 2 − , and potassium iodide (KI) for hole and OH radical. Remarkably, the absence of scavenger has shown better results almost 96.3% of PRA dye suspension yet, when used scavengers declined the removal percentage like KI-40.9%, BQ-44.5%, t-BuOH-42.7% as presented in ESI (Fig S3). www.nature.com/scientificreports/ Stability and recycling of TCA composite. In favour of investigate the reusability of synthesized TCA composite, the synthesized TCA was cleaned with milli Q water followed by ethanol and centrifuged to execute the later reaction. Significantly, the photodegradation of PRA dye is declined gradually up to five successive runs as 97, 96.1, 95.2, 94 and 92.7% for the successive five cycles respectively. This may be due to the loss of TCA during washing for recyclability. Fig S4(a) in ESI stated that prepared TCA composite acted as very dynamic photocatalyst up to five successive runs, thus the TCA photocatalyst remains stable and potent because of the presence of TANI surface to Cu 2 Oand Ag in synthesized composite that leads to mobilize the photogenerated electron and holes, so enhance the photocatalytic efficacy 41 . Fig S4(b) in ESI implies the XRD patterns of TCA which confirmed that no phase change was noticed in recycled TCA composite after reusable for successive five runs. From the obtained results, we strongly declared that polymerization synthesized TCA nanocomposite can be probably used as a stable photocatalyst for the dye (PRA) degradation in large scale.  Fig. 12. However, the polymer based composite exhibits more adsorption capacity due to the presence of more surface groups, greater adsorption capacity, good stability and mechanic viability 42 . In this study, three most common isotherms such as Freundlich, Langmuir and Temkin models are used to interpret the experimental adsorption data. The equilibrium adsorption isotherms were determined using batch studies. www.nature.com/scientificreports/ Freundlich model. According to Freundlich equation, the amount adsorbed increases infinitely with increasing concentration or pressure. The Freundlich Eq. (9) isotherm can be linearized into the following form:

Removal of cadmium ion (cd
The regression coefficient (R 2 ), slope and intercept were 0.94, 1.26 and 1.77 respectively. From these results, n and k F values were calculated to be 0.78 and 58.41 respectively (Fig. 12a).
Langmuir model. Langmuir isotherm Eq. (10) is derived from simple mass kinetics, assuming chemisorption. This model was depicted in Fig. 12b. From this graph, calculated the regression coefficient (R 2 ), slope and intercept were 0.93, 0.36 and − 1.13 respectively. From these results, Q max and k L are 2.73 and 0.32 respectively, desorption of the Cd 2+ is very low.
Temkin model. The linear relationship can be calculated from Eq. (11) and the result was depicted in Fig. 12c. The regression coefficient (R 2 ), slope and intercept were calculated to be 0.98, 5.35 and − 3.44 respectively. The adsorption capacity and adsorption intensities, Q e and ln k T ; 5.9 and 0.63 respectively obtained.
From these results, the Freundlich model was fitted for the adsorption of Cd 2+ onto ternary nanocomposite (Table 1).

Corrosion inhibition.
The corrosion resistance behaviour of synthesized pure TANI, TANI/Cu 2 O and TANI/Cu 2 O/Ag composite coatings were evaluated by sweeping the potential from equilibrium potential towards negative and positive potentials against Ag/AgCl reference electrode in H 2 SO 4 (1 M), HCl (1 M) and NaCl (3.5%, w/w) electrolytes using Tafel polarisation and electrochemical impedance spectroscopy techniques (Fig. 13). The trends of open-circuit potential curves provided clear evidence that the incorporation of Cu 2 O into TANI nanostructures is beneficial, as it introduces potential shift towards the noble positive potential for both binary and ternary composite coatings. But the ternary composite doesn't show the good corrosive resistance behaviour than binary composite because of low percent of Ag content in composite, which doesn't shows the adverse effect on corrosion inhibition. Also, the corrosion resistance was enhanced with increase in the Cu 2 O content in TANI supported nanocomposite coatings. Table 2 displays the values related to the corrosion current (I corr ), corrosion potential (E corr ), cathodic (β a ), anodic (β c ) slopes and corrosion rate (CR) were calculated from tafel plots for pure TANI, TANI/Cu 2 O and TANI/Cu 2 O/Ag nanocomposites, also their polarization resistance (R p ) was calculated from kinetic parameters obtained from Tafel plots as Stern-Geary Eq. 43 .
(9) log Q e = log k F + 1/n log C e (10) C e /Q e = 1/k L Q max + C e /Q max (11) Q e = B 1 ln k T + B 1 ln C e www.nature.com/scientificreports/ where β a and β c are the respective slopes of cathodic and anodic tafel plots. Fig S5 ( Figure S6 displays the photographic images of an inhibition zone was rendered by TANI/Cu 2 ONC against four different bacterial strains. The zone of inhibition of TANI/Cu 2 ONC for various microorganisms is shown in Table S3 (ESI). Among all the strains selected for this study, S.aureus is the most active strain (21 µg/mL) at the concentration of 80 µg/ mL. The synthesized TANI/Cu 2 ONC is effectively inhibited the bacterial growth and gram positive (S.aureus) is observed to be so effective than gram negative (S. typhimurium) bacteria.
Antimicrobial activity of TANI/Cu 2 O/Ag nanocomposite. The TANI/Cu 2 O/Ag NC was tested against the both gram positive and gram negative bacterial strains. Figure 14 shows the photographic images of an inhibition zone produced by TCA NC against four different bacterial strains. The zone of inhibition of TCA NC for various microorganisms was shown in Table 3. The influence TCA NC on S. aureus (25 µg/mL) and S.typhimurium (22 µg/mL) exhibited the best results at the examined concentration of 80 µg/mL. The prepared TCA NC effectively killing the bacterial growth and gram positive is observed to be so might effective than gram negative bacteria. The significant antimicrobial activity was not found by pure TANI and Cu 2 O. The binary and ternary composites show antimicrobial efficiency against both Gram-positive and negative bacteria. These results proposed two different mechanisms of ternary composite with target microorganism interaction, the first: adsorption of ternary composite to cell walls, membrane disruption and cell leakage which is mainly connected with high molecular weight ternary composite, the second: penetration of ternary composite into living cells leading to inhibition of various enzymes and interference with synthesis of mRNA and proteins. However, the actual mechanism of inhibition activity of TANI/Cu 2 O/Ag composite is not fully understood yet.
In present study, the antimicrobial performance confirmed that gram positive bacterial strains are more affected than gram negative (Fig S7) because Gram positive bacteria have thin peptidoglycan layer with abundant pores that permits foreign molecules to penetrate into cell and damage or malfunctioning the cell organisms. Gram positive and gram negative bacteria have differences in their membrane structure, the most distinctive of which is the thickness of the peptidoglycan layer. The high concentration of Cu 0 affected bactericidal effect. Therefore, copper nanoparticles are chosen as a carrier in this work 44 . A very few reports on Ag ions effects on microorganisms in which Ag + attracts the negatively charged cell membrane of microorganism through electrostatic interactions 45 . Hence, Ag + plays a vital role in the antibacterial activity of Ag in solutions with salts, zeolites and polymers 46 . For instance, the AgNP-polymeric nanoparticles showed increased antimicrobial properties towards E.coli and S.aureus 47 . The Ag NPs induced oxidative stress on bacteria and also damaged the cell membrane, protein, and DNA 48 . However, the Ag NPs can be easily penetrate to cell wall of microorganisms and also their greater surface area enhance the antimicrobial performance by bring a huge atoms into intact with the parts of bacteria 47 . Hence, the Ag NPs were used in the study, however, they received negative charge from TANI. The biological activity of Ag and Cu nanoparticles is also dependent on their size, causing penetration and damage of Gram-positive and negative bacteria cells 49 . The mechanism of the inhibitory effects of ternary composite on microorganisms is not yet known clearly. Further investigation was required.   Characterization. The diffraction peaks were recorded by powder X-Ray diffraction (XRD D8 Bruker AXS) with Cu-Kα radiation at 30.0 kV and 30.0 mA over the scan range (2θ) 10-90° at the scan rate of 2°/min. FTIR (IR Prestige21, Shimadzu Pvt Ltd, Japan) spectra of the samples in KBr pellets were obtained on a Shimadzu spectrometer in the range of 400-4,000 cm −1 . Morphology of the prepared materials was investigated by Scanning electron microscopy (FESEM, JSM-6610LV, Jeol Asia PTE Ltd, Japan.) and the elemental analysis was done by EDS. UV-Visible absorption and diffuse reflectance spectra were recorded by UV-Visible diffuse reflectance spectrometer (Shimadzu 2600R) in the range of 200-800 nm. The PL spectra of prepared composite were taken using Flouromax-4, HORIBA, Japan to elucidate the transfer behaviour of photo-induced electrons and holes. X-ray photoelectron spectroscopy (XPS) spectra obtained from Physical electronics PHI 5,000 versaprobe III with C60 ion gun to study the electron spectroscopy of TCA composite.
Photocatalytic studies. The photocatalytic performances of produced samples were conducted. The suspensions for the photocatalytic reaction were prepared by adding 0.03 g of various catalysts to a required concentration of dye and pollutant solutions. Before illumination, the mixture was magnetically stirred for 30 min in the dark to establish adsorption-desorption equilibrium of organic pollutants with the catalyst. A solar simulator with a metal halide lamp was used as the visible light source. The experimental solution was placed in a quartz cuvette, 100 mm away from the light source. 5 mL of the suspension was withdrawn at regular time intervals and centrifuged to remove the dispersed catalyst powder. The concentration of the clear solution was determined using UV-Visible absorption spectra. The photodegradation efficiency was determined by measuring the absorbance spectra of the dye solution before (C 0 ) and after the photocatalytic reaction with variation of time (C t ). The degradation efficiency of the photocatalytic reaction was calculated using Eq. (13) where C 0 or A 0 are initial concentration or absorbance and C t or A t after the photocatalytic reaction concentration or absorbance with various time (t).
Adsorption studies. The deletion of heavy metals like cadmium ions (Cd 2+ ) via adsorption process by insitu polymerisation synthesized nanocomposite. During this process, 0.03 g of the sorbent was added to 10 mg/L of Cd 2+ solution under stirring for 90 min in dark condition. Then, gathered the solution mixture at every 15 min time intervals which were further analysed by UV-Visible spectrophotometer. The amount of Cd 2+ adsorbed onto the ternary composite can be calculated by the mass balance relationship by the following Eq. (14).
where w is the weight of the adsorbent (g), and V is the volume of the solution (L).
Inhibition of corrosion analysis. The anticorrosion activity of as-prepared samples has been analysed for pitting corrosion and the test conducted through potentiodynamic polarization procedure using software based PAR weld electrochemical system of the GILL AC unit with the sample dimensions of 20 × 20 × 10 mm. A satu- www.nature.com/scientificreports/ rated calomel electrode and carbon electrode were used as reference and auxiliary electrodes respectively. All the experiments were piloted in aerated 3.5% NaCl solution with pH adjusted to 10 by adding potassium hydroxide. The exposure area for these experiments was 1 cm 2 and current has been inducted into an electrolyte. The pitting corrosion resistance of the samples has been determined by obtained E corr values, which is current equal to the potential at anode and cathode.
Inhibition of bacterial growth assay. The synthesized TANI and its binary and ternary nanocomposites were tested for antibacterial activity over the bacterial strains of Salmonella typhimurium (MTCC-3231). Shigella flexneri (MTCC-1457), Bacillus coagulans (MTCC-5856) and Staphylococcus aureus (MTCC-3160) grown overnight at 37 °C temperature and the medium was sterilized by autoclaving at120 °C (15 lb/in 2 ). The nutrient agar medium, 20 mL with the respective bacterial strains of bacteria were transferred aseptically into each sterilized Petri plate, which were allowed to cool at room temperature for solidification. Each plate was made into 5 wells with equal distance of 6 mm sterile borer. Chloramphenicol was taken as positive control for bacterial species. The antibacterial activity of the compound was evaluated by well-established Agar well diffusion method. The four wells were made and filled with concentrations of 40, 60 and 80 µg/mL of tested samples and then plates were incubated for a period of 24 h at 37 °C in incubator, after incubation the inhibitory zone formed around the well was measured.

Data availability
The data was generated and analyzed during the current study are available from the corresponding author on reasonable request.