Table 2 Identification and annotation of expressed candidate genes associated with the resistance to H. parviporum QTL regions in Norway spruce.

From: Combining transcriptomics and genetic linkage based information to identify candidate genes associated with Heterobasidion-resistance in Norway spruce

QTLIdentification of Norway spruce candidate genesAnnotation information
LGPositionTraitUnigenesCandidate genesBLASTNAnnotation (Blast X)E-valuesim mean (%)
1151.7IPsp_v3.0_unigene1013MA_10433492g00100alanine aminotransferase 2087.6
1151.7IPsp_v3.0_unigene5249MA_74668g00104E-144uncharacterized protein LOC18442845-like8.83715E-6271.9
1151.7IPsp_v3.0_unigene7019MA_8609304g00100RING-H2 finger ATL32.514E-13962.9
1151.7IPsp_v3.0_unigene9778MA_3162g00200.001probable E3 ubiquitin- ligase RNF2174.1795E-11562.3
1151.7IPsp_v3.0_unigene2934MA_138177g00101E-136probable rRNA-processing EBP2 homolog2.42956E-7971.5
1151.7IPsp_v3.0_unigene21078MA_110169g00100AAB01572.1 heat shock-like protein [Picea glauca]7.71175E-4181.9
1161.3Esp_v3.0_unigene380MA_106437g00100Protein of unknown function (DUF789)079.6
1161.3Esp_v3.0_unigene4974MA_125631g00100Kinesin motor domain091.4
1162.1Esp_v3.0_unigene36567MA_44157g00100DNAJ heat shock family protein1.78E-13598.5
1162.6Esp_v3.0_unigene11985MA_585559g00100Respiratory-chain NADH dehydrogenase 24 Kd subunit3.21E-9468.5
1162.6Esp_v3.0_unigene16970MA_90134g00107.30E-13160S acidic ribosomal protein family2.25E-3260.5
275.6IPsp_v3.0_unigene109795MA_186971g00105E-80UDP-glycosyltransferase family protein093.8
275.6IPsp_v3.0_unigene128417MA_138834g00102E-144aldehyde dehydrogenase family member094.4
275.6IPsp_v3.0_unigene18333MA_9478104g00100rmlC-like cupin domain-containing5.42113E-9699.4
275.6IPsp_v3.0_unigene35076MA_10434009g00100AHW42454.1 GA2ox4, partial [Pinus tabuliformis]089.6
281.7Esp_v3.0_unigene1167MA_114154g00101.10E-164Protein of unknown function (DUF1118)2.98E-6380.1
281.7Esp_v3.0_unigene17595MA_10437148g00100catalase 2098.6
281.7Esp_v3.0_unigene30559MA_10427042g00100RING-variant domain9.51E-0578.1
282.7Esp_v3.0_unigene11933MA_28209g00100NAD(P)-binding Rossmann-fold superfamily protein097.6
283.3Esp_v3.0_unigene182897MA_10428567g00101.40E-145Protein of unknown function (DUF679)2.04E-11392.5
284.6Esp_v3.0_unigene10696MA_6091g00106.40E-90–-NA–-6.63E-4089.9
285.5Esp_v3.0_unigene8898MA_10435680g00100Esterase/lipase/thioesterase family protein6.83E-16994.8
285.6Esp_v3.0_unigene126081MA_120550g00100Leucine-rich receptor-like protein kinase family protein1.10E-13885.5
286.2Esp_v3.0_unigene11941MA_215978g00100Eukaryotic aspartyl protease089.9
286.2Esp_v3.0_unigene353MA_140175g00100Protein kinase superfamily protein091.4
287.3Esp_v3.0_unigene126858MA_218996g00103.70E-128Oxygen evolving enhancer protein 3 (PsbQ)4.38E-6575.9
287.3Esp_v3.0_unigene17509MA_10436080g00100dehydroquinate dehydratase, putative / shikimate dehydrogenase, putative1.81E-0791.8
287.3Esp_v3.0_unigene17565MA_10426048g00201.70E-80alpha-galactosidase 21.63E-9485.7
287.3Esp_v3.0_unigene7904MA_10426424g00102.30E-134Isocitrate dehydrogenase kinase/phosphatase (AceK)1.34E-0578.6
289.2Esp_v3.0_unigene27550MA_48816g00100universal stress PHOS321.79E-6065.1
290.6Esp_v3.0_unigene30396MA_10431443g00100superoxide dismutase1.69E-6174.5
2130.8SWGsp_v3.0_unigene14476MA_129592g00100receptor-like protein kinase 21.79E-0657.7
2131.8SWGsp_v3.0_unigene22490MA_19032g00106.86E-102unknown3.03E-12990.4
2133.1SWGsp_v3.0_unigene8841MA_27441g00104E-56PREDICTED: glycerophosphodiester phosphodiesterase GDPD1, chloroplastic-like [Elaeis guineensis]6.62E-1762.1
2134.4SWGsp_v3.0_unigene127279MA_10427811g00101.3E-178SRG1-like1.7743E-15075.9
375Esp_v3.0_unigene97367MA_72133g00108.80E-82CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B8.73E-2898.3
375.7Esp_v3.0_unigene27952MA_1548g00100calmodulin-binding family protein085.0
376.4Esp_v3.0_unigene34116MA_15852g00100phenylalanine ammonia-lyase 22.70E-6479.4
632.9SWGsp_v3.0_unigene131072MA_10434621g00104.00E-103Ribosomal protein L32e7.95E-5091.4
641.1SWGsp_v3.0_unigene1862MA_10428976g00100PLAC8 family protein067.7
642.1SWGsp_v3.0_unigene126822MA_417026g00103.90E-118Isochorismatase family1.49E-8286
642.1SWGsp_v3.0_unigene6079MA_10430766g00100Apolipophorin-III precursor (apoLp-III)1.34E-8785.5
642.9SWGsp_v3.0_unigene210326MA_4442g00100acyl-CoA oxidase 3091.2
644.9SWGsp_v3.0_unigene209193MA_12029g00100Ribosomal protein L18ae/LX family protein2.12E-12598.3
6111.6Esp_v3.0_unigene18249MA_62177g00100Phosphofructokinase family protein3.83E-14590.9
6111.6Esp_v3.0_unigene34489MA_10429833g00100Protein of unknown function (DUF3529)2.34E-17294.4
6112Esp_v3.0_unigene23234MA_10264676g00105.00E-64–-NA–-1.27E-4285.9
6112.9Esp_v3.0_unigene2749MA_213048g00102.20E-153Histone superfamily protein2.14E-3165.6
6113.5Esp_v3.0_unigene23240MA_14341g00108.60E-113Protein of unknown function (DUF581)8.57E-6667.6
6115Esp_v3.0_unigene126925MA_5176g00100Myo-inositol-1-phosphate synthase0.00E + 0094.2
6115Esp_v3.0_unigene30267MA_101663g00105.50E-19Oligosaccaryltransferase1.45E-19100
6115.5Esp_v3.0_unigene16221MA_10436501g00101.80E-147unknown5.71E-6398.1
6115.5Esp_v3.0_unigene27539MA_10433186g00100Ribonuclease T2 family1.58E-15593.3
6117.1Esp_v3.0_unigene8468MA_14707g00101.18E-08ATP-dependent zinc metalloprotease FTSH chloroplastic088.9
6117.3Esp_v3.0_unigene3531MA_25056g00100NAP1-like protein3.9317E-113100.0
6117.9Esp_v3.0_unigene22292MA_264971g00105E-163ATAF1-like protein, partial [Picea mariana]3.042E-17297.2
6118.4Esp_v3.0_unigene6238MA_1881g00100acyl-coenzyme A oxidase peroxisomal082.0
6119.4Esp_v3.0_unigene6836MA_181986g00100Myb-like DNA-binding domain0.00E + 0096
6119.7Esp_v3.0_unigene20354MA_103386g00104E-92ATAF1-like protein, partial [Picea mariana]6.89E-13685.4
6119.7Esp_v3.0_unigene27482MA_7719740g00102.05E-134ABK24195.1 unknown [Picea sitchensis]099.6
6121.1Esp_v3.0_unigene22913MA_16728g00100transcription factor Trihelix family2.19134E-8963.0
6125.7Esp_v3.0_unigene15800MA_942991g00107E-13EFTu, similar to Arabidopsis thaliana At4g20360086.7
6125.7Esp_v3.0_unigene36222MA_10430642g00200.001beta-fructofuranosidase like3.52075E-6372.7
6125.9Esp_v3.0_unigene96752MA_86256g00102.24E-62ATAF1-like protein, partial [Picea mariana]3.54E-11579.9
6125.9Esp_v3.0_unigene11986MA_199901g00108E-131unknown, similar to similar to Arabidopsis thaliana At1g187207.46E-13398.5
6126.6Esp_v3.0_unigene30362MA_138328g00102.40E-88–-NA–-2.28E-3590.1
6126.6Esp_v3.0_unigene9511MA_140162g00100–-NA–-6.65E-11983.6
6127.2Esp_v3.0_unigene10286MA_96911g00100Core histone4.47E-11982.7
6127.3Esp_v3.0_unigene10376MA_3262g001005′-AMP-activated protein kinase beta-2 subunit protein5.16E-15296.4
6130.8Esp_v3.0_unigene23051MA_98205g00100F-box family protein0.00E + 0087.6
6130.8Esp_v3.0_unigene36722MA_120172g00104.60E-56Splicing factor 3B subunit 10 (SF3b10)1.25E-24100
6131.1Esp_v3.0_unigene4021MA_137415g00104.90E-96NAC domain containing protein 251.28E-3178.7
6131.9Esp_v3.0_unigene29556MA_219331g00100unknown0.00E + 0096.2
6132Esp_v3.0_unigene126785MA_10426681g00101.58E-85Major intrinsic protein2.19E-3288.6
6132Esp_v3.0_unigene29917MA_268790g00101.25E-07–-NA–-1.27E-7585.9
6132Esp_v3.0_unigene18689MA_9307243g00100acyl-transferase family protein0.00E + 0095.8
6132Esp_v3.0_unigene37742MA_915221g00100Pollen allergen1.53E-13888.6
6134.4Esp_v3.0_unigene18634MA_9055473g00100Haloacid dehalogenase-like hydrolase (HAD) superfamily protein0.00E + 0069.3
6135.8Esp_v3.0_unigene17528MA_15246g00100Zinc finger, C3HC4 type (RING finger)2.77E-11687.8
6141.6Esp_v3.0_unigene10892MA_10429538g00100Polyketide cyclase / dehydrase and lipid transport4.46E-12397.3
6141.6Esp_v3.0_unigene18104MA_86745g00100Thioredoxin5.41E-10998.2
6141.6Esp_v3.0_unigene202138MA_5692971g00104.60E-106–-NA–-5.56E-5395.5
6141.6Esp_v3.0_unigene34060MA_10433807g00100Multicopper oxidase1.48E-10881.1
6141.6Esp_v3.0_unigene34081MA_104063g00100Zinc finger, C3HC4 type (RING finger)2.34E-11372.1
6141.6Esp_v3.0_unigene36520MA_134293g00103.00E-89Cleavage site for pathogenic type III effector avirulence factor Avr3.40E-4077.1
6142.2Esp_v3.0_unigene40516MA_745622g00100phosphoglycerate kinase0.00E + 0099.1
6154SWGsp_v3.0_unigene18240MA_10436145g00103E-41F-box kelch-repeat At5g15710-like049.8
6154SWGsp_v3.0_unigene22702MA_10434415g00101E-20microtubule-associated RP EB family member2.6854E-13968.4
6154SWGsp_v3.0_unigene32084MA_10429904g00104E-85ABK22325.1 unknown [Picea sitchensis]1.56689E-7183.0
6154SWGsp_v3.0_unigene6593MA_10230764g00108E-17ABR17176.1|unknown [Picea sitchensis]6.1607E-13564.3
6154SWGsp_v3.0_unigene73878MA_131587g00100chlorophyll a b-binding8.8049E-16198.4
6154SWGsp_v3.0_unigene86259MA_853405g00101.91E-32hypothetical protein [Pinus taeda]3.0779E-3971.7
6154SWGsp_v3.0_unigene22630MA_248791g00102E-113unknown [Picea sitchensis]ABK23762.11.5646E-149100.0
6154SWGsp_v3.0_unigene18288MA_962052g00100PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein 3 [Prunus mume]1.67E-12084.9
6154SWGsp_v3.0_unigene13063MA_10426138g00101.23E-117dihydropyrimidinase isoform1.1E-14391.4
6154SWGsp_v3.0_unigene5833MA_122748g00108E-88Glutaredoxin099.2
8140.8LLsp_v3.0_unigene21297MA_184192g00100.00E + 00tubulin alpha-2 chain0.00E + 0092.6
8142.4LLsp_v3.0_unigene12232MA_52380g00100UDP-glucuronate 4-epimerase 3086.2
8143LLsp_v3.0_unigene10846MA_10435810g00100cinnamoyl- reductase 27.9337E-14079.8
8143.4LLsp_v3.0_unigene13707MA_107074g00103E-117F-box At5g51380-like1.2968E-18077.8
8143.4LLsp_v3.0_unigene17253MA_10432221g00108E-50adenine phosphoribosyltransferase like6.47166E-5194.2
8143.4LLsp_v3.0_unigene26050MA_10431633g00105E-14ADE77033.1 unknown [Picea sitchensis]7.0318E-14271.5
8144.7LLsp_v3.0_unigene7190MA_10296151g00100.00E + 00Carboxylesterase family0.00E + 0092.9
8145.3LLsp_v3.0_unigene61649MA_17691g00103.20E-113Leucine-rich repeat protein kinase family protein9.95E-0668.6
9122.4SWGsp_v3.0_unigene3494MA_10429563g00100Protein kinase domain6.62E-15598.2
9126.3SWGsp_v3.0_unigene38053MA_3650g00100CAB06080.1 porin [Picea abies]094.9
9126.3SWGsp_v3.0_unigene36841MA_19215g00100–-NA–-–-NA–-–-NA–-
9127.2SWGsp_v3.0_unigene30750MA_108756g00108.35E-65NRT3 family1.4193E-12270.2
9127.5SWGsp_v3.0_unigene182972MA_10435879g00104.62E-145NAD(P)-binding Rossmann-fold superfamily092.9
1130IPsp_v3.0_unigene18400MA_362678g00100S-adenosyl-L-methionine-dependent methyltransferases superfamily protein2.20E-16499.2
1130.1IPsp_v3.0_unigene30128MA_10018366g00105.95E-76–-NA–-3.84E-30100.0
1131.2IPsp_v3.0_unigene207722MA_427046g00101.00E-83Core histone H2A/H2B/H3/H41.56E-3695.7
1131.7IPsp_v3.0_unigene8310MA_215979g00101E-102ABK21694.1 unknown [Picea sitchensis]3.8437E-12398.8
1132.3IPsp_v3.0_unigene162285MA_24271g00201E-167R2R3-MYB transcription factor PgMYB11-like096.9
1132.3IPsp_v3.0_unigene15787MA_6931g00100.0000586caffeoyl CoA O-methyltransferase [Picea abies]1.9055E-16999.2
1132.4IPsp_v3.0_unigene18329MA_4742g00109E-111MFT1-like protein [Picea abies]1.783E-12599.4
1132.5IPsp_v3.0_unigene4041MA_7247276g00101E-152cytochrome P450 monooxygenase CYP736B095.5
1132.5IPsp_v3.0_unigene4998MA_10427673g00200cytochrome P450 CYP736A12-like3.03E-7573.6
1133.3IPsp_v3.0_unigene9613MA_14663g00200Cytochrome P450-like,098.4
1136.4IPsp_v3.0_unigene25068MA_498632g001002-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein5.85E-18086.3
1136.4IPsp_v3.0_unigene53315MA_10425962g00202.20E-992-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein2.69E-9190.1
1136.8IPsp_v3.0_unigene9722MA_28222g00101.80E-180Cytochrome P450 superfamily protein086.1
1140.1IPsp_v3.0_unigene36253MA_19954g00100unknown1.53E-1783.2
1140.1IPsp_v3.0_unigene9396MA_139843g00100Tetraspanin family4.37E-0779.2
  1. a Linkage groups are numbered according to Lind et al.14; b Position of QTL regions for traits controlling resistance to H. parviporum;c QTL regions for traits controlling resistance to H. parviporum as described in Lind et al.14: exclusion (E), infection prevention (IP), lesion length (LL), sapwood growth of fungus (SWG); d unigenes according to SustainPine v3.0;e unique candidate genes in Norway spruce genome Pabies v1.0; fblastN score of the best hit in Norway spruce genome Pabies v1.0; g Best BlastX hit of the Norway spruce candidate genes in Genbank nr protein database.