Correction to: Scientific Reports https://doi.org/10.1038/s41598-020-60622-1, published online 26 February 2020
The Article contains several typographical errors and omitted References in the Materials and Methods section under the subheading ‘PCR amplification and sequence analyses of 16 S rRNA, 18S rRNA, 23S rRNA and tufA’ where:
“PCR amplification and sequence analyses of 16 S rRNA, 18S rRNA, 23S rRNA and tufa
Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515 f (F), 5′-GTGCCAGCMGCCGCGGTAA-3′ and 806r (R), 5′-GGACTACHVHHHTWTCTAAT-3′] for 16S, TAReuk454FWD1 (F), 5′-CCAGCASCYGCGGTAATTC-3′ and TAReukREV3 (R), 5′-ACTTTCGTTCTTGATYRA-3′ primers for 18S rDNA, p23SrV_f1 (F), 5′-GGACAGAAAGACCCTATGAA-3′ and p23SrV_r1 (R), 5′-TCAGCCTGT-TATCCCTAGAG-3′ primers for 23S rDNA, (F) 5′-TGAAACAGAAMAWCGTCATT-3′ and (R) 5′-CCTTCNCGAATMGCRAAW-3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).”
should read:
“PCR amplification and sequence analyses of 16S rRNA, 18S rRNA, 23S rRNA and tufa
Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515f (F), 5′-GTGYCAGCMGCCGCGGTAA-3′1 and 806r (R), 5′-GGACTACNVGGGTWTCTAAT-3′2] for 16S, TAReuk454FWD1 (F), 5′-CCAGCASCYGCGGTAATTC-3′ and TAReukREV3 (R), 5′-ACTTTCGTTCTTGATYRA-3′ primers3 for 18S rDNA, p23SrV_f1 (F), 5′-GGACAGAAAGACCCTATGAA-3′ and p23SrV_r1 (R), 5′-TCAGCCTGTTATCCCTAGAG-3′ primers4 for 23S rDNA, (F) 5′-TGAAACAGAAMAWCGTCATT-3′ and (R) 5′-CCTTCNCGAATMGCRAAW-3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).”
The omitted References are listed below as References 1–4 respectively.
References
Parada, A. E., Needham, D. M. & Fuhrman, J. A. Every base matters: Assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ. Microbiol. 18(5), 1403–1414 (2016).
Apprill, A., McNally, S., Parsons, R. & Weber, L. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquat. Microb. Ecol. 75(2), 129–137 (2015).
Stoeck, T. et al. Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water. Mol. Ecol. 19, 21–31 (2010).
Sherwood, A. R. & Presting, G. G. Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria 1. J. Phycol. 43(3), 605–608 (2007).
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Ermis, H., Guven-Gulhan, U., Cakir, T. et al. Author Correction: Effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate. Sci Rep 10, 11963 (2020). https://doi.org/10.1038/s41598-020-69043-6
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DOI: https://doi.org/10.1038/s41598-020-69043-6
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