Tools of the trade: MicroCT reveals native structure and functional morphology of organs that drive caterpillar–ant interactions

Abstract

Caterpillars of many lycaenid butterflies are tended by ants that offer protection from predators and parasitoids. Specialized structures such as glands, ciliary organs and chitinous ornamentation in caterpillars play key roles in the underlying tactile, acoustic, and chemical communication between caterpillars and ants. Although the ecological, evolutionary, and behavioural aspects of these interactions are well studied, the mechanisms (i.e., the functional morphology) that drive the specialized interactive organs are poorly characterized. We used advanced X-ray microtomography (MicroCT) to delineate internal, native morphology of specialized larval dew patches, nectar glands, and tactile ciliary organs that mediate interactions between Crematogaster ants and caterpillars of the obligate myrmecophilous Apharitis lilacinus butterfly. Our non-destructive MicroCT analysis provided novel 3-D insights into the native structure and positions of these specialized organs in unmatched detail. This analysis also suggested a functional relationship between organ structures and surrounding muscles and nervation that operate the glands and tactile organs, including a ‘lasso bag’ control mechanism for dew patches and muscle control for other organs. This provided a holistic understanding of the organs that drive very close caterpillar–ant interactions. Our MicroCT analysis opens a door for similar structural and functional analysis of adaptive insect morphology.

Introduction

Association with ants, termed myrmecophily, is widespread among plants1,2,3, mollusks4, and arthropods5,6,7,8. Butterflies in the family Lycaenidae show extensive ant associations9,10,11, with ~ 75% of the approx. 5,200 species in the family being myrmecophiles12,13. Myrmecophilic associations of lycaenid caterpillars have resulted in facultative or obligate relationships that range from mutualism to parasitism, mediated by a spectrum from mutual rewards to behavioural manipulation14,15,16,17,18. Along this spectrum of interactions, lycaenid caterpillars have evolved specialized morphological features such as a thick cuticular dermis that protects them from ant aggression19, extended thoracic legs20, dew patches and nectar glands (Newcomer’s gland) that provide sugary rewards to attending ants, and tactile organs that manipulate ant behaviour21,22,23,24. Additionally, pheromone-secreting glands, called pore cupola, mediate caterpillar–ant interactions19.

These specialized organs function sequentially to modulate ant behaviour. The pore cupola organs secrete appeasement pheromones to subdue aggressive behaviour of ants in close proximity19,25. The dew patches and nectar glands produce carbohydrate-rich secretions that are devised to reward the tending ants, although in some cases the secretions may be sucked in by the caterpillar before ants can take them up, i.e., they may only lure but not always reward the ants, which is part of the larval strategy of deceipt19,22,26. The tactile eversible organs are positioned near either anterior or posterior end of caterpillars19,22,25. Additionally, sensory tufts of the tactile organs assist in sensing trail pheromones or alarmones of ants, directing caterpillars to move towards ant aggregations19,25. The suite of these specialized caterpillar structures and behaviours modulate ant behaviour in favour of caterpillars, creating a protective, enemy-free space27. These interactions and protective umbrellas extend to pupal stages, where myrmecophilous interactions are largely mediated by acoustic and chemical means28,29.

The evolution of these organs across Lycaenidae12,15 with respect to their roles in ecological30,31 and behavioural interactions18,27,28,29 has been explored to a considerable extent. However, the detailed internal structures or functional morphology of these organs is poorly characterized, barring some preliminary studies with limited tools19,22. The interaction of these organs with surrounding anatomical systems is also unknown. To address these limitations, we used advancements in microtomography (MicroCT), which enable fine understanding of internal and functional morphology32,33. MicroCT provides high resolution and three-dimensional reconstruction that enables detailed quantitative and qualitative characterization of hard as well as soft tissue in their native states, without requiring dissection or histology34,35,36,37. Therefore, we used MicroCT to characterize the internal morphology of specialized organs in the early stages (caterpillar and pupa) of Apharitis lilacinus, which has an obligate association with Crematogaster hodgsoni ants (Figs. 1 and S1a–c)38. Female A. lilacinus deposit eggs at the nest entrance of C. hodgsoni ants—sometimes on sand and away from plants. Caterpillars and then the pupae are completely dependent on ants from hatching to eclosion, and constantly attended (Fig. 1)38. Caterpillars do not feed on plant tissue at all, but only on regurgitated food provided by ants. It is possible that they also eat ant broods when available, but this needs to be confirmed. In any case, all stages of A. lilacinus have obligate association with ants, and they possess all the ant-associated organs described in obligate lycaenid myrmecophiles. Our MicroCT-assisted study revealed not only the fine structures of these organs, but also their functional morphology in relation to surrounding musculature and nervation, substantially advancing the understanding of the mechanistic basis of these caterpillar–ant interactions (Figs. 2a–o, 3a–i, and 4a–h; Supplementary Movie 1).

Figure 1
figure1

Apharitis lilacinus life cycle showing larval and pupal associations with Crematogaster hodgsoni ants. Structures of the specialized larval organs that mediate caterpillar–ant interactions are depicted on the right based on our MicroCT data (Figs. 2, 3, 4). Blue arrow in the nectar gland shows the external opening of the gland. The life stages, interactions, and MicroCT-derived 3D volumes of caterpillar and pupa of A. lilacinus are animated in Supplementary Movie 1. Image courtesy for egg: Nitin Ravikanthachari; pupa and ant-caterpillar/pupa interaction: Ashok Sengupta and G. S. Girish Kumar.

Figure 2
figure2

Dew patches of the A. lilacinus caterpillar. (a, b) external morphology in open (OpDP) and closed (ClDP) configurations of the dew patches; (cl) depth-wise projections showing muscle organization and gut in relation to the dew patches (Rmu retractor muscles, DoLoMu dorsal longitudinal muscles, TrSeMu trans-segmental muscles, Gca gland cavity, Sc external scales); (m, n) original grayscale slice delineating gland positions (marked with yellow lines); (o) a diagram of the dew patches showing closed and open configurations of glands (DPGl: dew patch gland).

Figure 3
figure3

Nectar glands of the A. lilacinus caterpillar. (a) External morphology; (bg) depth-wise projections showing internal organization of muscles (SpMu sphincter muscle, AcLoMu accessory longitudinal muscles, DoLoMu dorsal longitudinal muscle) and gut in relation to the nectar glands (marked with yellow dashed lines) and their temporary reservoir or gland cavity (Gca); (h) original grayscale slice delineating nectar glands; (i) a diagram of the nectar gland in relation to gut and sphincter muscle. Blue arrow points to the external opening of the gland.

Figure 4
figure4

Tactile organs of the A. lilacinus caterpillar. (a, b) External morphology; (cf) depth-wise projections showing internal organization of muscles (SpMu sphincter muscle), nerve fibres, and gut, in relation to the tactile organ chambers (marked with yellow dashed lines); (g) original grayscale slice delineating tactile organs; (h) a diagram showing open and closed configurations of tactile organs along with relative positions of gut, sphincter muscle, and the last abdominal ganglion.

Results and Discussion

The high magnification MicroCT scans provided sharp delineation of the specialized ant-associated organs, resolving structural features such as their native size and shape, gland mass, ducts, external orifices, and cuticular boundaries (Figs. 2a–o, 3a–i, and 4a–h; Supplementary Movie 1). The abdomen contained dew patches in the two anterior segments, and tactile organs and opening of nectar gland at the posterior end (Fig. S2a–c). It also revealed associated anatomical features such as musculature, major ganglia and neuronal connections at low magnification (Fig. S3a–f).

A ‘lasso bag’ control mechanism operates the ant-appeasing dew patches

The two dew patches, which release sugary secretions to appease tending ants, were positioned on the dorso-median line of the second and third abdominal segments (Figs. 3a–i and S3a–f). We show both closed and open configurations of the dew patches (ClDP and OpDP, respectively, in Fig. 2a–o). The gland openings were lined with specialized elongated scales (Sc), which completely covered the gland cleft when closed. The eccrine or apocrine gland lobules lined the inner cleft of the dew patches in both configurations (Fig. 2c–d). Lateral view of dew patches showed connecting ducts from each glandular lobule (marked in yellow dotted lines in Fig. 2a–o) opening into an eversible cleft (GCa), which likely acts as a temporary reservoir. In the closed configuration, the cleft became narrower and deeper, and the gland lobules lining the cleft sunk further inside the body, compared to the open configuration (Fig. 2i–j). The opening and closure of the dew patches would be controlled by associated dorso-ventral retractor muscles (RMu), connecting the lateral walls of the cleft with the ventral body-wall, encircling visceral organs.

These arrangements suggest a ‘lasso bag’ control mechanism for the dew patches, similar to pore cupola19. The retractor muscles should constrict to expand the gland cavity, pushing gland lobules closer to the surface and enhancing the surface area for secretion. While closing, the muscles should relax, sinking the gland lobules inside the body, closing the gland cavity, and decreasing the surface area to minimize exudate release (Fig. 2a–o).

Accessory longitudinal muscles control release and retraction of secretions from nectar glands that bait ants

Nectar glands feature prominently in obligate myrmecophilic caterpillars39. In A. lilacinus, they started at the junction of abdominal segments 5 and 6, and opened externally in a common cleft between segments 7–8, connected antero-laterally with flanking accessory dorsal longitudinal muscles (Fig. 3a–i). From the lateral view, the glands were parallel to the dorsal dermis anteriorly, and gradually bent upward towards the cleft opening. The nectar gland lobes were not clearly visible, but the single long, slender reservoir and ducts were resolved (marked in yellow-dotted lines in Fig. 3a–i).

The accessory muscles should normally constrict in response to ant palpation, possibly pushing contents of the gland reservoir into the cleft. The trans-segmental, accessory longitudinal, and sphincter muscles associated with the common cleft should control its opening, releasing the nectar gland secretions. The last abdominal ganglion is connected with all these muscles, which presumably orchestrates their actions to release or suck back nectar gland secretions while baiting the ants.

Muscles and haemolymph pressure likely operate tactile organs that engage ants

The tactile organs, as the name suggests, mediate interactions primarily through tactile responses22, although pyriform glandular cells at the base of sensilla contribute to chemical communication by producing pheromones19, thus together facilitating multi-modal communication. In A. lilacinus, we observed the paired tactile organs on the eighth abdominal segment (also see Fig. S2a–c) with a tuft of terminal sensilla on a whitish ciliary body everted when ants palpated. MicroCT showed that the tactile organs were multi-layered, chitinous tubes, covered with a thick dermal layer, their external orifices being annular (marked with yellow-dotted lines in Fig. 4d–f). They spanned nearly the height of the body, continuing till the ventro-lateral side of the hindgut, and were lateral to the sphincter muscles (Fig. 4d–g). The tufts of sensilla on the ciliary body were partially resolved (Fig. 4d).

Ant palpations presumably induce eversion of ciliary bodies, which is likely controlled by increased haemolymph pressure and ventral muscles that are connected with the last abdominal ganglion (Fig. 4h).

Other ant-associated morphology

Our MicroCT analysis revealed two other morphological features that contribute to myrmecophily of A. lilacinus caterpillars. Similar to other lycaenids19, A. lilacinus caterpillars had a thick, convoluted dermis (Fig. S3a–f) to protect against ant aggression. The pro-thoracic plate and endplate were also thick and chitinous (Fig. S3a–f), which presumably act as protective shields for the sensitive head and abdominal ends. Second, the foregut volume was remarkably small (Fig. S3a–f), which was potentially related to dependence on trophallaxis in the caterpillar, which obviate the heavy digestive machinery found in caterpillars that have to digest tough vegetation.

Finally, the pupae of obligate myrmecophiles interact with ants via substratum-borne acoustic signals that are produced by stridulating chitinous micro-ridges at the segmental ends28,29,40. However, in the obligate myrmecophilic A. lilacinus pupae, we did not observe stridulatory ridges in the high-resolution scan. Stridulatory ridges are perhaps highly inconspicuous in A. lilacinus pupae, or their ant associations are mediated purely by pheromones and other non-structural means. However, MicroCT delineated in fine detail all the standard external and internal morphological features of the pupa that are also seen in metamorphosed adults (Fig. S4a–f; Supplementary Movie 1).

The understanding of mechanisms underlying insect symbioses remains limited because their chemical and structural bases remain poorly characterized, often due to unavailability of adequate methods. Recently developed MicroCT techniques permit very fine characterization of internal structures of small organisms in native (natural) states, elucidating the physical bases of these adaptive behaviours and interactions in unprecedented detail. Our MicroCT data delineated both the external surface topology and fine internal anatomical organization of the caterpillar and pupa of the obligate myrmecophilous A. lilacinus butterfly. Additionally, this analysis suggested how the supporting musculature and nervation may operate these organs during interaction with host ants that protect and feed the caterpillars. This is the first time that the ant-associated larval organs that facilitate these interactions have been functionally characterized at micron levels.

Functional morphology of the specialized organs, interpreted from our MicroCT data, is instrumental in the ‘push and pull’ mechanism of ant interactions with A. lilacinus caterpillars. The functional synergy of these organs builds a stable myrmecophilous strategy in this butterfly species. Likewise, a comparative account of functional morphology of these organs in different myrmecophilous caterpillars from different points on the interaction spectrum—from opportunistic mutualism all the way to obligate parasitism—could significantly enrich our mechanistic understanding of these inter-order ant-lycaenid butterfly interactions. Such a detailed mechanistic understanding will provide valuable insights into the spectacular diversification of this super-diverse butterfly family.

Finally, this study opens up a door for other mechanistic studies of the functional morphology of hard and soft tissue that are critical in behavioural, ecological and developmental aspects of insect adaptations. Functional morphologies of insect adaptations have always been considered important but they have been difficult to study because of technological limitations. Applications of similar MicroCT analysis may include a wide spectrum from mechanics of soft tissue in insect larvae and relative organ development through insect metamorphosis, to predator–prey interactions and arms-races.

Methods

Specimen collection

Apharitis lilacinus is an obligate myrmecophile, whose caterpillars live initially in the ant nests and later under the bark of Acacia plants38. It is legally protected in India under Schedule II of the WildLife (Protection) Act, 1972. As per the research and collection permit from the Karnataka Forest Department (see Acknowledgements), we collected two caterpillars from Bangalore, and raised them at room temperature and humidity in a plastic box along with a secondary Crematogaster hodgsoni ant colony under an Acacia bark.

Sample preparation

We standardized a previous sample preparation protocol for MicroCT scanning34, for our butterfly samples. As per our protocol, we fixed larval and pupal samples in a solution of Bouin’s fluid (saturated solution of picric acid in 95% 80 ml ethanol, 37–40% 15 ml formaldehyde, and 5 ml glacial acetic acid) for 24 h at 26 °C. After that, we kept the samples in water to rinse excess Bouin’s solution, and then gradually dehydrated them with serially diluted ethanol solutions (50–100% w/v) for 15 min in each stage. We then stained the samples from 100% ethanol solution with ethanol-dissolved 5% iodine for 24 h. We submerged the samples in ethanol to leech excess iodine in two stages, each lasting 30 min. We performed critical point drying (CPD) of the samples. We then mounted the samples using two methods that are appropriate for two types of MicroCT scanning: (a) low magnification scanning to study overall structures, for which we mounted samples surrounded by polyurethane foams on a standard double-sided tape as the base, on a brass holder, and (b) for high magnification scanning to study detailed internal morphology, we mounted specimens with Fevicol glue—a synthetic, thermoplastic resin made from Polyvinyl acetate (Pidilite Industries Ltd.)—on a wooden holder.

X-ray microtomography (MicroCT scanning)

We performed tomographic scans at the NCBS Electron Microscopy Facility. We scanned the samples in a Skyscan1272 high-resolution microtomographer (Bruker MicroCT, Kontich, Belgium) with a Hamamatsu L11871_20 X-ray source and a XIMEA xiRAY16 16-megapixel camera with a 7.4 µm pixel size. We used the following scanning parameters for larval scans: (a) for low magnification scan: isotropic voxel size of 2.2 µm/pixel, source voltage 40 kV, the source current 250 µA, and image rotation scan 180° with a 0.55° rotation step, and scanned in three steps, which were combined into a single volume with a scan duration of 16 m: 46 s, and (b) for high magnification scan: isotropic voxel size of 0.65 µm/pixel, source voltage 40 kV, the source current 100 µA, and image rotation scan 180° with a 0.4° rotation step, and scanned in six steps, which were combined into a single volume with a scan duration of 1 h:0 m:16 s. We scanned the pupa with the following scanning parameters: (a) for low magnification scan: isotropic voxel size of 7.5 µm/pixel, source voltage 40 kV, the source current 250 µA, and image rotation scan 180° with a 0.6° rotation step, and scanned in two steps, which were combined into a 3D volume with scan duration of 8 m: 53 s, and (b) for high magnification scan: isotropic voxel size of 1.25 µm/pixel, source voltage 54 kV, the source current 85 µA, and image rotation scan 360° with a 0.4° rotation step, and scanned in five steps, which were combined into a 3D volume with a scan duration of 1 h:19 m:57 s. We used three Bruker MicroCT Skyscan system software (NRecon, Dataviewer, CTAn) to stitch scanned steps into single 3D volume, set threshold, reorient, shrink wrap region of interest (ROIs), and obtain the final datasets of cross-sectional images (2D slices).

3D rendering

We rendered the 3D volumes using CTVox (Bruker) (Figs. 2a–o, 3a–i, 4a–h, and S1a–c, S2a–c, S3a–f, S4a–f; Supplementary Movie 1). We rendered the pupal morphology using Dragonfly (ORS Inc.) to get enhanced surface contours. We generated sets of discrete transfer function ranges for pseudo coloration to best resolve external and internal structures based on innate signal and contrast of the grayscale slices.

Illustrations

We prepared illustrations of ant-associated larval organs (Figs. 1, 2a–o, 3a–i, and 4a–h) by tracing on the original projections from volume data in Adobe Illustrator CS5 (Adobe Systems Incorporated).

References

  1. 1.

    Janzen, D. H. Coevolution of mutualism between ants and Acacias in central America. Evolution (N. Y.) 20, 249–275 (1966).

    Google Scholar 

  2. 2.

    Heil, M. & McKey, D. Protective ant-plant interactions as model systems in ecological and evolutionary research. Annu. Rev. Ecol. Evol. Syst. 34, 425–553 (2003).

    Article  Google Scholar 

  3. 3.

    Rico-Gray, V. & Oliveira, P. The Ecology and Evolution of Ant-Plant Interactions (The University of Chicago Press, Chicago, 2007).

    Google Scholar 

  4. 4.

    Witte, V., Janssen, R., Eppenstein, A. & Maschwitz, U. Allopeas myrmekophilos (Gastropoda, Pulmonata), the first myrmecophilous mollusc living in colonies of the ponerine army ant Leptogenys distinguenda (Formicidae, Ponerinae). Insectes Soc. 49, 301–305 (2002).

    Article  Google Scholar 

  5. 5.

    Nault, L. R., Montgomery, M. E. & Bowers, W. S. Ant-aphid association: role of aphid alarm pheromone. Science 192, 1349–1351 (1976).

    ADS  CAS  Article  Google Scholar 

  6. 6.

    Ito, F. & Takaku, G. Obligate myrmecophily in an oribatid mite : novel symbiont of ants in the oriental tropics. Naturwissenschaften 81, 180–182 (1994).

    ADS  Article  Google Scholar 

  7. 7.

    Cushing, P. E. Myrmecomorphy and myrmecophily in spiders: a review. Fla. Entomol. 80, 165–193 (1997).

    Article  Google Scholar 

  8. 8.

    Lapeva-Gjonova, A. Ant-associated beetle Fauna in Bulgaria: a review and new data. Psyche A J. Entomol. 2013, 1–14 (2013).

    Article  Google Scholar 

  9. 9.

    Pierce, N. E. Butterfly-ant mutualism. In Toward a More Exact Ecology. The 30th Symposium of The British Ecological Society Vol. 480 (eds Grubb, P. J. & Whittaker, J. B.) (Blackwell Scientific Publications, Oxford, 1989).

    Google Scholar 

  10. 10.

    Kaminski, L., Mota, L., Freitas, A. & Moreira, G. Two ways to be a myrmecophilous butterfly: natural history and comparative immature-stage morphology of two species of Theope (Lepidoptera: Riodinidae). Biol. J. Linn. Soc. 108, 844–870 (2002).

    Article  Google Scholar 

  11. 11.

    Torres, P. J. & Pomerantz, A. F. Butterfly kleptoparasitism and first account of immature stages, myrmecophily, and bamboo host plant of the Metalmark Adelotypa annulifera (Riodinidae). J. Lepid. Soc. 70, 130–138 (2016).

    Google Scholar 

  12. 12.

    Pierce, N. E. et al. The ecology and evolution of ant association in the Lycaenidae (Lepidoptera). Annu. Rev. Entomol. 47, 733–771 (2002).

    CAS  Article  Google Scholar 

  13. 13.

    Fiedler, K. Ant-associates of Palaearctic lycaenid butterfly larvae (Hymenoptera: Formicidae; Lepidoptera: Lycaenidae)—a review. Myrmecol. Nachr. 9, 77–87 (2006).

    Google Scholar 

  14. 14.

    Cottrell, C. B. Aphytophagy in butterflies: its relationship to myrmecophily. Zool. J. Linn. Soc. Lond. 80, 1–57 (1984).

    Article  Google Scholar 

  15. 15.

    Pierce, N. E. The evolution and biogeography of associations between lycaenid butterflies and ants. In Oxford Surveys in Evolutionary Biology Vol. 60 (eds Loxdale, H. D. et al.) 371 (Oxford University Press, Oxford, 1987).

    Google Scholar 

  16. 16.

    Pierce, N. E. & Young, W. R. Lycaenid butterflies and ants: two-species stable equilibria in mutualistic, commensal, and parasitic interactions. Am. Nat. 128, 216–227 (1987).

    Article  Google Scholar 

  17. 17.

    Pierce, N. E. Predatory and parasitic Lepidoptera: carnivores living on plants. J. Lepid. Soc. 49, 412–453 (1995).

    Google Scholar 

  18. 18.

    Elgar, M. A., Nash, D. R. & Pierce, N. E. Eavesdropping on cooperative communication within an ant-butterfly mutualism. Naturwissenschaften 103, 84 (2016).

    Article  Google Scholar 

  19. 19.

    Malicky, H. New aspects of the association between lycaenid larvae (Lycaenidae) and ants (Formicidae, Hymenoptera). J. Lepid. Soc. 24, 190–202 (1970).

    Google Scholar 

  20. 20.

    DeVries, P. J. & Penz, C. M. Entomophagy, behavior, and elongated thoracic legs in the myrmecophilous neotropical butterfly Alesa amesis (Riodinidae). Biotropica 32, 712–721 (2006).

    Article  Google Scholar 

  21. 21.

    Malicky, H. Versuch einer Analyse der ökologischen Beziehungen zwischen Lycaeniden (Lepidoptera) und Formiciden (Hymenoptera). Tijdschr. Entomol. 112, 213–298 (1969).

    Google Scholar 

  22. 22.

    Vegliante, F. & Hasenfuss, I. Morphology and diversity of exocrine glands in lepidopteran larvae. Annu. Rev. Entomol. 57, 187–204 (2011).

    Article  Google Scholar 

  23. 23.

    Fiedler, K., Hölldobler, B. & Seufert, P. Butterflies and ants: the communicative domain. Experientia 52, 14–24 (1996).

    CAS  Article  Google Scholar 

  24. 24.

    Devries, P. J., Harvey, D. J. & Kitching, I. J. The ant associated epidermal organs on the larva of the lycaenid butterfly Curetis regula Evans. J. Nat. Hist. 20, 621–633 (1986).

    Article  Google Scholar 

  25. 25.

    Fiedler, K. & Maschwitz, U. Functional analysis of the myrmecophilous relationships between ants (Hymenoptera: Formicidae) and lycaenids (Lepidoptera: Lycaenidae). Ethology 31, 409–416 (1987).

    Google Scholar 

  26. 26.

    Newcomer, E. J. Some observations on the relations of ants and lycænid caterpillars, and a description of the relational organs of the latter. J. N. Y. Entomol. Soc. 20, 31–36 (1912).

    Google Scholar 

  27. 27.

    Atsatt, P. R. Lycaenid butterflies and ants: selection for enemy-free Space. Am. Nat. 118, 638–654 (2002).

    Article  Google Scholar 

  28. 28.

    Travassos, M. A. & Pierce, N. E. Acoustics, context and function of vibrational signalling in a butterfly-ant mutualism. Anim. Behav. 60, 13–26 (2000).

    CAS  Article  Google Scholar 

  29. 29.

    DeVries, P. J. Enhancement of symbiosis between butterfly caterpillars and ants by vibrational communication. Science 248, 1104–1106 (1990).

    ADS  CAS  Article  Google Scholar 

  30. 30.

    Baylis, M. & Pierce, N. E. The effects of ant mutualism on the foraging and diet of lycaenid caterpillars. In Caterpillars. Ecological and Evolutionary Constraints on Foraging (eds Stamp, N. E. & Casey, T. M.) 404–421 ( Chapman and Hall, London, 1993).

    Google Scholar 

  31. 31.

    Baylis, M. & Pierce, N. E. Lack of compensation by final instar larvae of the myrmecophilous lycaenid butterfly, Jalmenus evagoras, for the loss of nutrients to ants. Physiol. Entomol. 17, 107–114 (1992).

    Article  Google Scholar 

  32. 32.

    Lowe, T., Garwood, R. J., Simonsen, T. J., Bradley, R. S. & Withers, P. J. Metamorphosis revealed: time-lapse three-dimensional imaging inside a living chrysalis. J. R. Soc. Interface https://doi.org/10.1098/rsif.2013.0304 (2013).

    Article  PubMed  PubMed Central  Google Scholar 

  33. 33.

    van de Kamp, T. et al. Parasitoid biology preserved in mineralized fossils. Nat. Commun. 9, 3325 (2018).

    ADS  Article  Google Scholar 

  34. 34.

    Castejón, D. et al. Micro-computed tomography and histology to explore internal morphology in decapod larvae. Sci. Rep. 8, 1–11 (2018).

    Article  Google Scholar 

  35. 35.

    Metscher, B. D. MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues. BMC Physiol. 9, 11 (2009).

    Article  Google Scholar 

  36. 36.

    Semple, T. L., Peakall, R. & Tatarnic, N. J. A comprehensive and user-friendly framework for 3D-data visualisation in invertebrates and other organisms. J. Morphol. 280, 223–231 (2019).

    Article  Google Scholar 

  37. 37.

    Hita Garcia, F. et al. X-ray microtomography for ant taxonomy: An exploration and case study with two new Terataner (Hymenoptera, Formicidae, Myrmicinae) species from Madagascar. PLoS ONE 12, e0172641 (2017).

    Article  Google Scholar 

  38. 38.

    Sengupta, A., Nitin, R., Girish Kumar, G. S. & Nagraj, V. Apharitis lilacinus (Moore, 1884) – Lilac Silverline. Butterflies of India, v. 2.71. Indian Foundation for Butterflies. Kunte, K., S. Sondhi, and P. Roy (Chief Editors) (2019). Available at: https://www.ifoundbutterflies.org/#!/sp/2125/Apharitis-lilacinus. (Accessed: 3rd October 2019).

  39. 39.

    Clark, G. & Dickson, C. G. C. The honey gland and tubercles of larvae of the Lycaenidae. Lepid. News 10, 37–42 (1956).

    Google Scholar 

  40. 40.

    Downey, J. & Allyn, A. Sound production and associated abdominal structures in pupae of Lycaenidae and Riodinidae. Bull. Allyn Mus. 14, 1–48 (1973).

    Google Scholar 

Download references

Acknowledgements

We thank Nitin Ravikanthachari, Ashok Sengupta and G. S. Girish Kumar for assistance in field work; Ashok Sengupta for providing a clip of A. lilacinus in nature for the Supplementary Movie 1; Sunil P. for assistance in MicroCT scanning; Mike Marsh of Object Research Systems (ORS Inc.) for providing a non-commercial license of the segmentation software Dragonfly; Karnataka Forest Department for research and specimen collection permit (No. 227/2014-2015, dated 2015/04/16), for which we thank the Principal Chief Conservator of Forest and field officers of the state; NCBS Electron Microscopy Facility; and NCBS Research Collections and Tarun Karmakar for specimen curation. This research was partially funded by a Ramanujan Fellowship from the Dept. of Science and Technology, Govt. of India, and an NCBS research grant to KK, a graduate Research Fellowship from the Council of Scientific and Industrial Research, Govt. of India, to DNB, and support of the Dept. of Atomic Energy, Govt. of India, under Project Nos. 12-R&D-TFR-5.04-0800 and 12-R&D-TFR-5.04-0900 to TIFR/NCBS.

Author information

Affiliations

Authors

Contributions

D.N.B. and K.K. conceived the project, designed experiments and wrote the manuscript. D.N.B. collected and analysed the MicroCT data. KK coordinated research.

Corresponding authors

Correspondence to Dipendra Nath Basu or Krushnamegh Kunte.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Basu, D.N., Kunte, K. Tools of the trade: MicroCT reveals native structure and functional morphology of organs that drive caterpillar–ant interactions. Sci Rep 10, 10593 (2020). https://doi.org/10.1038/s41598-020-67486-5

Download citation

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Sign up for the Nature Briefing newsletter for a daily update on COVID-19 science.
Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing