Abstract
Spodoptera frugiperda is one of the main pests of maize and cotton in Brazil and has increased its occurrence on soybean. Field-evolved resistance of this species to Cry1 Bacillus thuringiensis (Bt) proteins expressed in maize has been characterized in Brazil, Argentina, Puerto Rico and southeastern U.S. Here, we conducted studies to evaluate the survival and development of S. frugiperda strains that are susceptible, selected for resistance to Bt-maize single (Cry1F) or pyramided (Cry1F/Cry1A.105/Cry2Ab2) events and F1 hybrids of the selected and susceptible strains (heterozygotes) on DAS-444Ø6-6 × DAS-81419-2 soybean with tolerance to 2,4-d, glyphosate and ammonium glufosinate herbicides (event DAS-444Ø6-6) and insect-resistant due to expression of Cry1Ac and Cry1F Bt proteins (event DAS-81419-2). Susceptible insects of S. frugiperda did not survive on Cry1Ac/Cry1F-soybean. However, homozygous-resistant and heterozygous insects were able to survive and emerge as fertile adults when fed on Cry1Ac/Cry1F-soybean, suggesting that the resistance is partially recessive. Life history studies revealed that homozygous-resistant insects had similar development, reproductive performance, net reproductive rate, intrinsic and finite rates of population increase on Cry1Ac/Cry1F-soybean and non-Bt soybean. In contrast, heterozygotes had their fertility life table parameters significantly reduced on Cry1Ac/Cry1F-soybean. Therefore, the selection of S. frugiperda for resistance to single and pyramided Bt maize can result in cross-crop resistance to DAS-444Ø6-6 × DAS-81419-2 soybean. The importance of these results to integrated pest management (IPM) and insect resistance management (IRM) programs is discussed.
Similar content being viewed by others
Introduction
Transgenic plants expressing insecticidal proteins from Bacillus thuringiensis Berliner (Bt) have significantly contributed to IPM programs worldwide in the last decades1,2,3,4. Brazil is one of the largest adopter of biotech crops that express Bt proteins in the world, with approximately 36 million hectares of cultivated area during the 2017/2018 season, representing 62, 79 and 82% of the total area planted with soybean, maize and cotton, respectively4.
Brazil was also the first country in the world to approve the commercial release of Bt-soybean expressing the Cry1Ac protein (event MON87701 × MON89788)5, which has been cultivated since 2013/2014 season. This biotech event provided control of important soybean pests, such as Anticarsia gemmatalis (Lepidoptera: Erebidae), Chrysodeixis includens, Chloridea virescens and Helicoverpa armigera (Lepidoptera: Noctuidae)6,7,8,9,10. Recently, a new Bt soybean (event DAS-444Ø6-6 × DAS-81419-2) was approved for commercialization in Brazil11. The DAS-444Ø6-6 event (Enlist E3, Corteva Agriscience, Wilmington, DE) expresses the enzymes 5-enolpyruvyl shikimate-3-phosphate synthase (2mEPSPS), phosphinothricin acetyltransferase (PAT), and aryloxyalkanoate dioxygenase 12 (AAD-12) that confer tolerance to the herbicides glyphosate, glufosinate ammonium, and 2,4-dichlorophenoxyacetic acid (2,4-d), respectively. The DAS-81419-2 event (Conkesta, Corteva Agriscience, Wilmington, DE) consists of insect-resistant technology that expresses Cry1Ac and Cry1F Bt proteins11,12, and PAT that confers tolerance to the herbicide glufosinate ammonium as a selectable marker. Under field conditions, this Bt soybean provides protection against A. gemmatalis, C. includens, C. virescens and H. armigera13,14.
In Brazilian soybean fields there has been an increase in the occurrence of Spodoptera species, mainly Spodoptera frugiperda (Lepidoptera: Noctuidae)15,16,17—one the main lepidopteran pest of maize (Zea mays L.) and cotton (Gossypium hirsutum L.)18,19. Their occurrence in soybean can be explained by their ability to develop in several cultivated plants20,21, adult dispersal22, reproductive capacity, multiple generations per year23 and the Brazilian crop production system where there is an overlap of cultivated host plants (i.e. maize, cotton, sorghum, rice and soybean)24. These biological characteristics associated with the crop production landscapes favors the infestation of this pest on distinct cultivated host plants throughout the seasons.
Field-evolved resistance of S. frugiperda to Cry1F25 and Cry1Ab26 proteins in Brazil resulted in high survival rates on maize and cotton plants expressing pyramided Bt proteins27,28. Field-evolved resistance to Cry1F maize has also been documented in Puerto Rico29, some areas of the southeastern region of the mainland United States30, and Argentina31. The resistance of S. frugiperda to Cry1 proteins expressed in maize negatively affected the performance of Bt cotton technologies32,33, due to cross-resistance between Bt proteins34,35,36,37. Therefore, the resistance of S. frugiperda to Bt proteins is the main threat to the sustainability of current and future Bt plants used in IPM programs in Brazil.
In the Brazilian crop production landscapes, S. frugiperda resistant to Bt proteins will also be exposed to DAS-444Ø6-6 × DAS-81419-2 soybean. Therefore, evaluating the ability of S. frugiperda strains that have been selected for resistance to Bt maize to survive and develop on Cry1Ac/Cry1F-soybean is essential to support IPM and IRM programs. Here, we present data on the survival and development of S. frugiperda strains selected for resistance to single and pyramided Bt maize, as well as F1 hybrids between these and a susceptible strain (assumed to be heterozygous for Bt resistance), on DAS-444Ø6-6 × DAS-81419-2 soybeans (hereafter Cry1Ac/Cry1F-soybean).
Results
Plant tissue bioassays
Homozygous-resistant S. frugiperda from P-R (selected for resistance to Cry1F/Cry1A.105/Cry2Ab2) and H-R (selected for resistance to Cry1F) strains had similar mortality, stunting and larval weights when fed on Cry1Ac/Cry1F-soybean and non-Bt soybean (Table 1). In contrast, heterozygous larvae from reciprocal crosses (P-R♀ × Sus♂ and H-R♀ × Sus♂) showed a significant higher mortality on Cry1Ac/Cry1F-soybean (68 and 67%) than on non-Bt soybean (< 7% mortality). More than 70% of heterozygous larvae on Cry1Ac/Cry1F-soybean did not reach third instar at five days and larval weights were reduced by more than 50% compared to the same strains on non-Bt soybean. The susceptible strain (Sus) had higher mortality (97 and 82%), stunting and weight reduction on Cry1Ac/Cry1F-soybean than on non-Bt soybean (2 and 8% mortality and stunting, respectively) (Table 1).
Life history traits of S. frugiperda strains on Cry1Ac/Cry1F-soybean
No significant differences in the duration and survival of egg and pupal stages of homozygous-resistant (P-R and H-R) and heterozygous insects on Cry1Ac/Cry1F-soybean and non-Bt soybean were detected (Fig. 1). However, larval stage duration of P-R and H-R strains were significantly shorter (~ 2 days) on Cry1Ac/Cry1F-soybean, and this also reduced the egg-to-adult period when compared to the same strains developing on non-Bt soybean. Larval and egg-to-adult survival of P-R strain were significantly lower on Cry1Ac/Cry1F-soybean, while H-R strain showed higher survival on non-Bt (Fig. 1). For heterozygotes, the duration of larval (25 and 24 days) and egg-to-adult (43 and 38 days) periods was longer on Cry1Ac/Cry1F-soybean than non-Bt (18 and 33 days, respectively) (Fig. 1). However, the survival of heterozygous larvae on Cry1Ac/Cry1F-soybean was lower than 35%, while on non-Bt was higher than 95%. There was also a reduction in the number of heterozygous insects that completed the life cycle on Cry1Ac/Cry1F-soybean (24% reaching the adult stage), when compared to non-Bt (more than 80% originated adults). In contrast, susceptible insects did not survive until adult stage on Cry1Ac/Cry1F-soybean, while on non-Bt more than 60% developed into adults in 35 days. When fed on the same plant (Supplementary Fig. S1), P-R insects had higher survival from neonate to adult on Cry1Ac/Cry1F-soybean (82%) than H-R (67%), heterozygous (28%) and Sus (no survival) insects. On non-Bt soybean, the survival of P-R and heterozygous (higher than 88%) insects were similar, while H-R and Sus insects had a lower survival (58% and 75%, respectively). Based on previous results, the dominance levels (DML) of resistance of S. frugiperda on Cry1Ac/Cry1F-soybean were 0.32 (95% CI 0.29–0.36) and 0.40 (95% CI 0.37–0.43) for P-R and H-R strains, respectively, indicating that the resistance is partially recessive.
Life history traits of S. frugiperda strains on leaves of Cry1Ac/Cry1F-soybean (event DAS-444Ø6-6 × DAS-81419-2) and non-Bt soybean (isoline). Pairs of bars (± SE) with different letters differ significantly by t-test (P < 0.05). P-R strain (selected for resistance to Cry1F/Cry1A.105/Cry2Ab2-maize), H-R strain (selected for resistance to Cry1F-maize), and Sus strain (susceptible of reference).
No significant differences were detected in the larval weights of P-R and H-R insects on Cry1Ac/Cry1F-soybean and non-Bt soybean (Table 2). By contrast, pupal weight of resistant insects was significantly heavier on Cry1Ac/Cry1F-soybean than on non-Bt. Heterozygous larvae from both resistant strains presented lower weight on Cry1Ac/Cry1F-soybean, but the progeny from P-R♀ × Sus♂ had similar pupal weight on Bt and non-Bt soybean, while the insects from H-R♀ × Sus♂ had higher pupal weight on non-Bt. P-R females produced similar number of eggs when larvae developed on Cry1Ac/Cry1F-soybean or non-Bt, while H-R females produced more eggs when their development occurred on Bt-soybean. Females from P-R♀ × Sus♂ fed on Cry1Ac/Cry1F-soybean oviposited less eggs than on non-Bt. On the other hand, females from H-R♀ × Sus♂ presented similar number of eggs on Bt and non-Bt soybean. On the same host plant, larval weight was higher for resistant insects on Cry1Ac/Cry1F-soybean than other strains (Supplementary Fig. S2). In contrast, on non-Bt soybean heterozygotes had higher larval weight. Pupae were heavier for the H-R insects on Cry1Ac/Cry1F-soybean and H-R♀ × Sus♂ on non-Bt. Resistant and heterozygous females on Cry1Ac/Cry1F-soybean produced a similar number of eggs. However, on non-Bt heterozygous females produced more eggs.
Fertility life table parameters of P-R strain on the mean generation time, net reproductive rate, intrinsic and finite rate of population increase was similar on Cry1Ac/Cry1F-soybean and non-Bt (Table 3). Based on this, after ~ 40 days, 257 and 326 females from each P-R female are expected on Cry1Ac/Cry1F-soybean and non-Bt, respectively. However, H-R females presented higher fertility life table parameters on Bt soybean. For this strain, after ~ 40 days, 297 females/female are expected when feeding on Cry1Ac/Cry1F-soybean, while on non-Bt only 157 females/female in 45 days. By contrast, heterozygotes on Cry1Ac/Cry1F-soybean had their life history parameters negatively affected. For these insects, 94 and 105 females are expected from each female on Cry1Ac/Cry1F-soybean in 44 to 49 days, while on non-Bt soybean more than 540 females/female would be produced in 39 days. This represents a reduction of 80% in the number of females produced per generation on Cry1Ac/Cry1F-soybean. When fertility life table parameters were compared in a same host plant, the homozygous-resistant insects on Cry1Ac/Cry1F-soybean presented shortest generation time, better reproductive performance and rate of population increase (Supplementary Table S1).
Discussion
The colonies of S. frugiperda selected for resistance to single- and pyramided-Bt maize technologies showed high survival on Cry1Ac/Cry1F-soybean (event DAS-444Ø6-6 × DAS-81419-2). While quantitative measurements of Cry1Ac/Cry1F Bt protein expression were not collected in our study, observations from previous studies have shown that Cry1Ac/Cry1F protein expression in greenhouse-grown plants does fall within the range of expression observed across field environments (Corteva Agriscience, unpublished data). Measurements of Bt protein expression can vary across environments and be influenced by multiple factors, such as growth stage, position within the plant canopy, and type of plant tissue. The amount of Cry1Ac and Cry1F protein expressed in this soybean technology was reported in the USDA petition for nonregulated status38 and by De Cerqueira et al.39. Therefore, the high survival of resistant strains on Cry1Ac/Cry1F-soybean can be explained by the cross-resistance between Cry1 proteins expressed in Bt plants34,35,36,37 and their low natural susceptibility to Cry1Ac protein as reported in studies with Cry1Ac-cotton32,40,41,42, Cry1Ac-soybean43,44, and diet bioassays containing Cry1Ac43,45. The cross-resistance among Cry1 proteins is attributed to their similar amino acid sequence36 and also their same binding sites in the midgut of S. frugiperda35. Previous studies also showed that S. frugiperda resistant to Bt maize survived on single and pyramided Bt cotton32,33, indicating cross-crop resistance. Our results also revealed that homozygous-resistant insects had similar development and reproductive performance on Cry1Ac/Cry1F-soybean and non-Bt soybean. These finding indicate that resistant insects have no adaptive disadvantage in the absence of the selection agent, maintaining the resistance frequency in the field46.
By contrast, heterozygous insects showed lower survival than homozygous-resistant insects on Cry1Ac/Cry1F-soybean, but produced fertile adults. The survival of heterozygous insects on Cry1Ac/Cry1F-soybean indicated that the dominance levels of resistance is characterized as partially recessive for both resistant strains evaluated. In other words, it demonstrates that the Cry1Ac/Cry1F-soybean does not meet the high-dose definition (Bt protein expression that cause more than 95% mortality of heterozygotes)47 for S. frugiperda. A similar degree of resistance was reported in S. frugiperda strains selected for resistance to Bt maize when fed on leaf tissue of Cry1F-maize29,31,48, and cotton events expressing Cry1Ac/Cry1F, Cry1Ac/Cry2Ab2, and Cry1Ab/Cry2Ae Bt proteins32,33. The survival of heterozygous larvae on Cry1Ac/Cry1F-soybean also contributes to maintaining the resistance allele to Cry1 proteins in field populations. On the other hand, heterozygotes on Cry1Ac/Cry1F-soybean had lower larval weight and longer development time until adults. This feature could be exploited in IPM programs by increasing the exposure on the plant to beneficial arthropods or entomopathogenic agents. Unlike to previous results, the susceptible S. frugiperda had complete mortality on Cry1Ac/Cry1F-soybean, due to its high susceptibility to Cry1F protein, as previous reported before the field-evolved resistance of this species to Cry1F-maize25,27,28,29,30,31,32,33,34.
In the current Brazilian crop production landscapes, with successive cultivation of maize, cotton and soybean, S. frugiperda populations are exposed to high selection pressure for resistance to Bt proteins. Resistance has been observed in S. frugiperda field populations to several Bt proteins expressed in maize (i.e. Cry1F, Cry1Ab and Cry1A.105) in Brazil25,26,27. Currently, field populations of S. frugiperda are composed predominantly of insects carrying Cry1Ab, Cry1F, and Cry1A.105 resistance alleles26,28,49,50,51, reflected by the increasing use of insecticide applications in fields cultivated with crops expressing these proteins52. For example, on single or pyramided Bt maize technologies expressing Cry1 and Cry2 proteins up to four insecticidal sprays may be needed to manage S. frugiperda, under extreme infestations52,53. Based on this, it is expected that Cry1Ac/Cry1F-soybean may not provide stand-alone protection against S. frugiperda under Brazilian field conditions, making this species a non-target pest of this Bt technology. However, Cry1Ac/Cry1F-soybean was developed and does provide efficacy against the key soybean pests (A. gemmatalis, C. includens, and C. virescens) under field conditions in Brazil13,14, which are the driver pests for the development of Bt traits in soybean. Therefore, to maintain the effectiveness of Cry1Ac/Cry1F-soybean over time against the key soybean pests, the adoption of a structured refuge (20% of cultivated area should be planted with non-Bt soybean) will be important for delaying or preventing resistance evolution33,36,47.
According to our results, alternative IPM strategies will be necessary to control S. frugiperda on Cry1Ac/Cry1F-soybean. Therefore, monitoring the presence of larvae and the damage to Cry1Ac/Cry1F-soybean are essential for supporting decision making regarding the use of other IPM tactics. The use of chemical insecticides probably will be the main tactic against S. frugiperda on Bt soybean. However, Cry1Ac/Cry1F-soybean could also be integrated with biological control agents as baculovirus-based insecticides (e.g. Spodoptera frugiperda multiple nucleopolyhedrovirus—SfMNPV)54 and natural enemies55. In summary, IPM and IRM programs that integrate multiple control tactics with diverse mortality factors, rather than just relying on wide scale use of single control tactics like Bt crops, are needed to ensure the sustainability of Bt crops56 in Brazil, where the resistance of S. frugiperda to Cry1 Bt proteins is already widespread.
Methods
Description of S. frugiperda strains
Two putative S. frugiperda resistant colonies were selected from a field population collected in maize in Paulínia, São Paulo, Brazil (22° 42′ 38′′ S and 47° 06′ 26′′ W) using the F2 screen method developed by Andow and Alstad57. The selection and rearing of resistant colonies was described in detail by Muraro et al.53. The homozygous-resistant strains used in this study were H-R (selected for resistance to Cry1F-maize) and P-R (selected for resistance to Cry1F/Cry1A.105/Cry2Ab2-maize). We also used a strain of S. frugiperda that has been maintained in the laboratory since 2012 without exposure to Bt proteins. This population was collected in non-Bt maize during the 2011–2012 crop season in Mogi Mirim, São Paulo, Brazil (22° 28′ 31′′ S and 46° 54′ 21′′ W). We refer to this colony as a susceptible strain (Sus). To evaluate putative heterozygous insects, the crossing between resistant♀ × susceptible♂ were performed. We only used heterozygotes from this cross because inheritance of resistance is autosomal inherited, and heterozygous larvae have demonstrated similar mortality-response to Bt proteins in diet and leaf bioassays25,27,28,29,33,53.
Soybean plants
Seeds from Cry1Ac/Cry1F-soybean and non-Bt soybean (isoline) (maturity group 5.0) were sown in 12-l plastic pots (four seeds/pot) in a greenhouse. Before the bioassays, Bt and non-Bt plants were tested for Bt protein expression using detection kits for Cry1Ac and Cry1F (Envirologix, QuickStix).
Plant tissue bioassays
Bioassays were performed with soybean leaves from Cry1Ac/Cry1F-soybean and non-Bt soybean (isoline) in V5–6 and R4–5 growth stages. Leaves were removed from the upper third part of the plants and, in the laboratory, were placed on a gelled mixture of agar-water at 2.5% in 100 ml plastic cups. Subsequently, neonates from resistant or susceptible strains or their F1 hybrid were placed on each cup. Cups were sealed and maintained in a room at 25 ± 2 °C, 60 ± 10% RH, and a photophase of 14 h. The experimental design was completely randomized with 10 replicates of 10 neonates/strain/growth stage. Mortality, stunting (larvae that did not reach the 3rd instar) and weight were assessed after 5 days.
Life history traits of S. frugiperda strains on Cry1Ac/Cry1F-soybean
Homozygous-resistant, heterozygous or susceptible neonates were reared on leaves of Cry1Ac/Cry1F-soybean and non-Bt soybean (isoline) excised from greenhouse-grown plants at the R1 growth stage. In the laboratory, leaves were cut into pieces and placed on a gelled mixture of agar-water at 2.5% in 50 ml plastic cups. Then, a single neonate (6 replicates of 10 neonates/strain/treatment) was placed in each cup. Leaves were replaced every 48 h and cups were maintained in the same environmental conditions described above. The following life history traits were evaluated: duration and survival of egg, larva, pupa and total cycle periods (egg-to-adult); larval weight at 14 days; pupae weight 24 h after pupal formation; and number of eggs per female. The number of eggs were assessed daily from 18 couples kept in PVC cages (23-cm height × 10-cm diameter) internally coated with a paper towel and closed at the top with a voile-type fabric. To determine the embryonic period and survival, 100 eggs of the 2nd oviposition were obtained from each couple. The eggs were observed daily and the number of hatched larvae was counted.
Data analysis
The number of insects tested, dead and those did not develop to 3rd instar on Cry1Ac/Cry1F-soybean and non-Bt soybean (isoline) were used to estimate 95% confidence intervals (CIs) for the probability of mortality and stunting, according to a binomial distribution. For these analyses, the function binom.probit from the package binom in R 3.1.0 (R Development Core Team 2014)58,59 was used. Percent mortality and stunting were considered significantly different when the 95% CIs on Bt-soybean did not overlap the 95% CIs on non-Bt soybean. The life history data of S. frugiperda strains on Bt and non-Bt soybean met the assumptions of normality and homogeneity of variances, and were compared by t-test using the PROC TTEST procedure in SAS 9.160. Mortality until adult stage were used to estimate the effective dominance of resistance (DML) using the method described by Bourguet et al.61 based on the following equation: DML = (MRS – MSS)/(MRR – MSS), where MSS, MRR and MRS are the mortalities of the susceptible, resistant and heterozygous strains, respectively, on Bt-soybean. This equation estimates dominance levels on a scale of 0 to 1 (0 = complete recessivity and 1 = complete dominance). The 95% CIs for these estimates was calculated as proposed by Misra62. A fertility life table was also calculated by estimating the mean generation time (T), the net reproductive rate (Ro), and the intrinsic (rm) and finite (λ) rate of increases by the jackknife technique using “lifetable.sas” procedure developed by Maia et al.63 in SAS 9.160.
References
Kathage, J. & Qaim, M. Economic impacts and impact dynamics of Bt (Bacillus thuringiensis) cotton in India. Proc. Natl. Acad. Sci. USA 109, 11652–11656 (2012).
Brookes, G. & Barfoot, P. Global income and production impacts of using GM crop technology 1996–2015. GM Crops Food 8, 156–193 (2017).
Brookes, G. The farm level economic and environmental contribution of Intacta soybeans in South America: the first five years. GM Crops Food 9, 140–151 (2018).
Biotechnology Information Council. Economic and Socio-environmental Impacts of Insect Resistance Technology in Brazil: Historical Analysis, Perspectives and Future Challenges. (accessed 10 November 2019); https://cib.rds.land/impacts-of-insect-resistance-technology-br (CIB, 2018).
CTNBio. Technical Opinion No. 2542/2010-Commercial Release of genetically-modified insect resistant and herbicide tolerant soybean containing genetically-modified events MON 87701 and MON 89788. (accessed 10 November 2019); https://ctnbio.mcti.gov.br/documents/566529/686342/Parecer+T%C3%A9cnico+n%C2%BA%202542.2010.pdf/6d208b44-aa9c-4dd9-af7d-1de1ef8d1b92?version=1.0 (2010).
Bernardi, O. et al. Assessment of the high dose concept and level of control provided by MON 87701 × MON 89788 soybean against Anticarsia gemmatalis and Pseudoplusia includens (Lepidoptera: Noctuidae) in Brazil. Pest Manage. Sci. 68, 1083–1091 (2012).
Yano, S. A. C. et al. High susceptibility and low resistance allele frequency of Chrysodeixis includens (Lepidoptera: Noctuidae) field populations to Cry1Ac in Brazil. Pest Manag. Sci. 72, 1578–1584 (2015).
Rodrigues-Silva, N. et al. Negative cross-resistance between structurally different Bacillus thuringiensis toxins may favor resistance management of soybean looper in transgenic Bt cultivars. Sci. Rep. 9, 199 (2019).
Bernardi, O. et al. High levels of biological activity of Cry1Ac protein expressed on MON 87701 × MON 89788 soybean against Heliothis virescens (Lepidoptera: Noctuidae). Pest Manage. Sci. 70, 588–594 (2014).
Dourado, P. M. et al. High susceptibility to Cry1Ac and low resistance allele frequency reduce the risk of resistance of Helicoverpa armigera to Bt soybean in Brazil. PLoS ONE 11, e0161388 (2016).
CTNBio. Technical Opinion No. 5148/2016—commercial release of insect resistant soybean (Glycine max L.) ammonium glufosinate tolerant. Event: DAS-81419-2. (accessed 10 November 2019); https://ctnbio.mcti.gov.br/documents/566529/2051619/Parecer+T%C3%A9cnico+n%C2%BA%205148+-+2016/ae981978-fb55-4861-827a-73c736477c78 (2016).
Brandon, J. F., Ariane, C. S., Tempest, Y. J., Brian, L. P. & Rod, A. H. Insect-protected event DAS-81419-2 soybean (Glycine max L.) grown in the United States and Brazil is compositionally equivalent to nontransgenic soybean. J. Agric. Food Chem. 63, 2063–2073 (2015).
Marques, L. H. et al. Efficacy of soybean’s event DAS-81419-2 expressing Cry1F and Cry1Ac to manage key tropical lepidopteran pests under field conditions in Brazil. J. Econ. Entomol. 109, 1922–1928 (2016).
Marques, L. H. et al. Field evaluation of soybean transgenic event DAS-81419-2 expressing Cry1F and Cry1Ac proteins for the control of secondary lepidopteran pests in Brazil. Crop Prot. 96, 109–115 (2017).
Bueno, R. C. O. F., Bueno, A. F., Moscardi, F., Parra, J. R. P. & Hoffmann-Campo, C. B. Lepidopteran larva consumption of soybean foliage: basis for developing multiple-species economic thresholds for pest management decisions. Pest Manage. Sci. 67, 170–174 (2011).
Justiniano, W., Fernandes, M. G. & Viana, C. L. T. P. Diversity, composition and population dynamics of arthropods in the genetically modified soybeans roundup ready RR1 (GT 40-3-2) and intacta RR2 PRO (MON87701 × MON89788). J. Agric. Sci. 6, 33–44 (2014).
Conte, O. et al. Resultados do manejo integrado de pragas da soja na safra 2017/18 no Paraná. (accessed 21November 2019); https://www.infoteca.cnptia.embrapa.br/infoteca/bitstream/doc/1095318/1/Doc402OL.pdf (2018).
Pogue, M. G. A world revision of the genus Spodoptera Guenee (Lepidoptera: Noctuidae). Mem. Am. Entomol. Soc. 43, 1–202 (2002).
Martinelli, S., Barata, R. M., Zucchi, M. I., Silva-Filho, M. C. & Omoto, C. Molecular variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) populations associated to maize and cotton crops in Brazil. J. Econ. Entomol. 99, 519–526 (2006).
Busato, O. R. et al. Compared biology of Spodoptera frugiperda (Lepidoptera: Noctuidae) populations in corn and rice leaves. Neotrop. Entomol. 34, 743–750 (2005).
Barros, E. M., Torres, J. B. & Bueno, A. F. Oviposition, development, and reproduction of Spodoptera frugiperda (Lepidoptera: Noctuidae) fed on different hosts of economic importance. Neotrop. Entomol. 39, 996–1001 (2010).
Nagoshi, R. N. et al. Haplotype profile comparisons between Spodoptera frugiperda (Lepidoptera: Noctuidae) populations from Mexico with those from Puerto Rico, South America, and the United States and their implications to migratory behavior. J. Econ. Entomol. 108, 135–144 (2015).
Fitt, G. P. et al. Resistance risks of Bt cotton and their management in Brazil. In Environmental risk assessment of genetically modified organisms, volume 2, Methodologies for assessing Bt cotton in Brazil (eds Hilbeck, A. et al.) 300–345 (CAB International, Wallingford, 2006).
Barros, E. M., Torres, J. B., Ruberson, J. R. & Oliveira, M. D. Development of Spodoptera frugiperda on different hosts and damage to reproductive structures in cotton. Entomol. Exp. Appl. 137, 237–245 (2010).
Farias, J. R. et al. Field-evolved resistance to Cry1F maize by Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil. Crop Prot. 64, 150–158 (2014).
Omoto, C. et al. Field-evolved resistance to Cry1Ab maize by Spodoptera frugiperda in Brazil. Pest Manage. Sci. 72, 1727–1736 (2016).
Santos-Amaya, O. F. et al. Resistance to dual-gene Bt maize in Spodoptera frugiperda: selection, inheritance, and cross-resistance to other transgenic events. Sci. Rep. 5, 18243 (2015).
Bernardi, D. et al. Cross-resistance between Cry1 proteins in fall armyworm (Spodoptera frugiperda) may affect the durability of current pyramided Bt maize hybrids in Brazil. PLoS ONE 10, e0140130 (2015).
Storer, N. P. et al. Discovery and characterization of field resistance to Bt maize: Spodoptera frugiperda (Lepidoptera: Noctuidae) in Puerto Rico. J. Econ. Entomol. 103, 1031–1038 (2010).
Huang, F. et al. Cry1F resistance in fall armyworm Spodoptera frugiperda: single gene versus pyramided Bt maize. PLoS ONE 9, e0112958 (2014).
Chandrasena, D. I. et al. Characterization of field-evolved resistance to Bacillus thuringiensis-derived Cry1F δ-endotoxin in Spodoptera frugiperda populations from Argentina. Pest Manage. Sci. 74, 746–754 (2018).
Yang, F. et al. Performance and cross-crop resistance of Cry1F-maize selected Spodoptera frugiperda on transgenic Bt cotton: implications for resistance management. Sci. Rep. 6, 28059 (2016).
Horikoshi, R. J. et al. Effective dominance of resistance of Spodoptera frugiperda to Bt maize and cotton varieties: implications for resistance management. Sci. Rep. 6, 34864 (2016).
Vélez, A. et al. Inheritance of Cry1F resistance, cross-resistance and frequency of resistant alleles in Spodoptera frugiperda (Lepidoptera: Noctuidae). Bull. Entomol. Res. 103, 700–713 (2013).
Hernandez-Rodriguez, C., Hernandez-Martinez, P., Van Rie, J., Escriche, B. & Ferre, J. Shared midgut binding sites for Cry1A 105, Cry1Aa, Cry1Ab, Cry1Ac and Cry1Fa proteins from Bacillus thuringiensis in two important corn pests, Ostrinia nubilalis and Spodoptera frugiperda. PLoS ONE 8, e0068164 (2013).
Carrière, Y., Crickmore, N. & Tabashnik, B. E. Optimizing pyramided transgenic Bt crops for sustainable pest management. Nat. Biotechnol. 33, 161–168 (2015).
Yang, A., Kerns, D. L., Graham, H., Brown, S. & Huang, F. Susceptibility of Cry1F-maize resistant, heterozygous, and susceptible Spodoptera frugiperda to Bt proteins used in the transgenic cotton. Crop Prot. 98, 128–135 (2017).
USDA Petition for determination of nonregulated status for insect-resistant DAS-81419-2 soybean. (accessed 29 May 2020); https://www.aphis.usda.gov/brs/aphisdocs/12_27201p.pdf.
De Cerqueira, D., Fast, B. J., Silveira, A. C. & Herman, R. A. Transgene-product expression levels in genetically engineered breeding stacks are equivalent to those of the single events. GM Crops Food 10, 35–43 (2019).
Sivasupramaniam, S. et al. Toxicity and characterization of cotton expressing Bacillus thuringiensis Cry1Ac and Cry2Ab2 proteins for control of lepidopteran pests. J. Econ. Entomol. 101, 546–554 (2008).
Adamczyk, J. J. et al. Evaluation of Bollgard, Bollgard II, and WideStrike technologies against beet and fall armyworm larvae (Lepidoptera: Noctuidae). Fla. Entomol. 91, 531–536 (2008).
Sorgatto, R. J., Bernardi, O. & Omoto, C. Survival and development of Spodoptera frugiperda and Chrysodeixis includens (Lepidoptera: Noctuidae) on Bt cotton and implications for resistance management strategies in Brazil. Environ. Entomol. 44, 186–192 (2015).
Bernardi, O. et al. Low susceptibility of Spodoptera cosmioides, Spodoptera eridania and Spodoptera frugiperda (Lepidoptera: Noctuidae) to genetically-modified soybean expressing Cry1Ac protein. Crop Prot. 58, 33–40 (2014).
Murúa, M. G., Vera, M. A., Herrero, M. I., Fogliata, S. V. & Michel, A. Defoliation of soybean expressing Cry1Ac by Lepidopteran pests. Insects 9, 83 (2018).
Santos, K. B. et al. Selection and characterization of the Bacillus thuringiensis strains toxic to Spodoptera eridania (Cramer), Spodoptera cosmioides (Walker) and Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae). Biol. Control 50, 157–163 (2009).
Gassmann, A. J., Carrière, Y. & Tabashnik, B. E. Fitness costs of insect resistance to Bacillus thuringiensis. Ann. Rev. Entomol. 54, 147–163 (2009).
Andow, D. A. The risk of resistance evolution in insects to transgenic insecticidal crops. Collect. Biosaf. Rev. 4, 142–199 (2008).
Farias, J. R. et al. Dominance of a Cry1F resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) on TC1507 Bt maize in Brazil. Pest Manage. Sci. 72, 974–979 (2015).
Farias, J. R. et al. Frequency of Cry1F resistance alleles in Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil. Pest Manage. Sci. 72, 2295–2302 (2016).
Santos-Amaya, O. F. et al. Magnitude and allele frequency of Cry1F resistance in field populations of the fall armyworm (Lepidoptera: Noctuidae) in Brazil. J. Econ. Entomol. 110, 1770–1778 (2017).
Farias, J. R., Horikoshi, R. J., Santos, A. C. & Omoto, C. Geographical and temporal variability in susceptibility to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda (Lepidoptera: Noctuidae) populations in Brazil. J. Econ. Entomol. 107, 2182–2189 (2014).
Burtet, L. M. et al. Managing fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), with Bt maize and insecticides in southern Brazil. Pest Manage. Sci. 73, 2569–2577 (2017).
Muraro, D. S. et al. Laboratory and field survival of Spodoptera frugiperda (Lepidoptera: Noctuidae) on Bt and non-Bt maize and its susceptibility to insecticides. Pest Manage. Sci. 75, 2202–2210 (2019).
Bentivenha, J. P. et al. Baseline susceptibility of Spodoptera frugiperda (Lepidoptera: Noctuidae) to SfMNPV and evaluation of cross-resistance to major insecticides and Bt proteins. J. Econ. Entomol. 112, 91–98 (2018).
Pomari, A. F., de Bueno, A. F., de Bueno, R. C. O. F. & de Menezes, A. O. Biological characteristics and thermal requirements of the biological control agent Telenomus remus (Hymenoptera: Platygastridae) reared on eggs of different species of the genus Spodoptera (Lepidoptera: Noctuidae). Ann. Entomol. Soc. Am. 105, 73–81 (2012).
Hutchison, W. D. Insect resistance management and integrated pest management for Bt crops: prospects for an area-wide view. In Bt resistance: characterization and strategies for GM crops producing Bacillus thuringiensis toxins (eds Soberón, M. et al.) 186–201 (CAB International, Wallingford, 2015).
Andow, D. A. & Alstad, D. N. F2 screen for rare resistance alleles. J. Econ. Entomol. 91, 572–578 (1998).
R Development Core Team, R. A Language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. (accessed 22 October 2019); https://www.R-project.org/. (2014).
Dorai-Raj, S. binom: BINOMIAL confidence intervals for several parameterizations. R package version 1.0–5. (accessed 22 October 2019); https://CRAN.R-project.org/package_binom. (2009).
SAS Institute Inc., SAS/STAT: 9.1. Statistical Analysis System: getting started with the SAS learning. Cary, NC: SAS Institute (2000).
Bourguet, D., Genissel, A. & Raymond, M. Insecticide resistance and dominance levels. J. Econ. Entomol. 93, 1588–1595 (2000).
Misra, R. K. Statistical tests of hypotheses concerning the degree of dominance in monofactorial inheritance. Biometrics 24, 429–434 (1968).
Maia, A. H. N., Luiz, A. J. B. & Campanhola, C. Statistical inference on associated fertility life table parameters using jackknife technique: computational aspects. J. Econ. Entomol. 93, 511–518 (2000).
Acknowledgements
This work was supported by National Council for the Improvement of Higher Education (CAPES) that provide a scholarship to the first author. The field population of S. frugiperda used in this study was registered in the National System for the Management of Genetic Heritage and Associated Traditional Knowledge (SisGen number AF999FD).
Author information
Authors and Affiliations
Contributions
E.P.M., L.H.M., A.C.S., T.N., M.L.D., C.O. and O.B. conceived and designed the studies; E.P.M., G.L.S.R.J., F.F., S.L.Z. selected the resistant colonies, performed experiments and collected data; E.P.M. and O.B. analyzed the data; E.P.M., L.H.M., A.C.S., T.N., M.L.D., C.O. and O.B. interpreted the results, discussed, and wrote the manuscript; E.P.M., L.H.M. and O.B. coordinated the project. All authors read and approved the final manuscript.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Machado, E.P., dos S. Rodrigues Junior, G.L., Führ, F.M. et al. Cross-crop resistance of Spodoptera frugiperda selected on Bt maize to genetically-modified soybean expressing Cry1Ac and Cry1F proteins in Brazil. Sci Rep 10, 10080 (2020). https://doi.org/10.1038/s41598-020-67339-1
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-020-67339-1
This article is cited by
-
Optimization of In Vivo Production of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)
Neotropical Entomology (2022)
-
Integrative taxonomy and phylogeography of Telenomus remus (Scelionidae), with the first record of natural parasitism of Spodoptera spp. in Brazil
Scientific Reports (2021)
-
Large-scale assessment of lepidopteran soybean pests and efficacy of Cry1Ac soybean in Brazil
Scientific Reports (2021)
-
Ecological Modelling of Insect Movement in Cropping Systems
Neotropical Entomology (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
