Blood pressure-lowering effects of a Bowman-Birk inhibitor and its derived peptides in normotensive and hypertensive rats

Bioactive plant peptides have received considerable interest as potential antihypertensive agents with potentially fewer side effects than antihypertensive drugs. Here, the blood pressure-lowering effects of the Bowman-Birk protease inhibitor, BTCI, and its derived peptides, PepChy and PepTry, were investigated using normotensive (Wistar-WR) and spontaneously hypertensive rats (SHR). BTCI inhibited the proteases trypsin and chymotrypsin, respectively, at 6 µM and 40 µM, a 10-fold greater inhibition than observed with PepTry (60 µM) and PepChy (400 µM). These molecules also inhibited angiotensin converting enzyme (ACE) with IC50 values of 54.6 ± 2.9; 24.7 ± 1.1; and 24.4 ± 1.1 µM, respectively, occluding its catalytic site, as indicated by molecular docking simulation, mainly for PepChy and PepTry. Gavage administration of BTCI and the peptides promoted a decrease of systolic and diastolic blood pressure and an increase of renal and aortic vascular conductance. These effects were more expressive in SHR than in WR. Additionally, BTCI, PepChy and PepTry promoted coronary vasodilation and negative inotropic effects in isolated perfused hearts. The nitric oxide synthase inhibitor blunted the BTCI and PepChy, with no cardiac effects on PepTry. The findings of this study indicate a therapeutic potential of BTCI and its related peptides in the treatment of hypertension.


Inhibition assay of BTCI, PepTry and PepChy against trypsin and chymotrypsin. The inhibitory
activities of BTCI and its derived peptides, PepTry and PepChy, were evaluated through the residual enzymatic activity of serine proteases trypsin and chymotrypsin in the presence of increasing concentrations of BTCI and its derived peptides ( Fig. 2A-D). In the absence of BTCI and peptides, the proteases cleave BAPNA/GPNA substrates releasing the yellow-colored p-nitroanilide product corresponding to an enzymatic activity of 100% 39 . In the presence of BTCI and peptides, a gradual decay of the protease activities is observed. BTCI at approximately 6 µM and 40 µM inhibits trypsin and chymotrypsin, respectively, a 10-fold greater inhibition than for PepTry (60 µM) and PepChy (400 µM). The inhibition constants, Ki, of BTCI and PepTry against trypsin were 0.59 ± 0.03 × 10 −8 M 16 and 1.19 ± 0.04 × 10 −7 M, respectively; Ki values against chymotrypsin for BTCI and PepChy were 1.15 ± 0.04 × 10 −7 M 17 , and 1.21 ± 0.26 × 10 −4 M, respectively. These values indicate that BTCI is a more potent inhibitor against trypsin than chymotrypsin, with PepTry more potent than PepChy.
In the present study, the ACE-inhibitory activity of BTCI and its derived peptides, PepChy and PepTry was evaluated at different concentrations (0-600 µM) as shown in Fig. 3. The ACE-inhibitory activity increased in a dose-dependent pattern. The highest inhibitory value of about 98% was recorded for PepTry and PepChy at 100 µM, whereas approximately 300 µM of BTCI was required to reach 94% of ACE-inhibition. The ACE-inhibition curve fitting was conducted by a non-linear method, from which the IC 50 values for BTCI, PepChy and PepTry were estimated as 54.6 ± 2.9 µM, 24.7 ± 1.1 µM, and 24.4 ± 1.1 µM, respectively.
The ACE-inhibitory effects of PepChy and PepTry are consistent with molecular docking simulation. This methodology was used to predict the interactions of the inhibitors PepChy and PepTry with ACE by calculating interaction energies for the best pose of inhibitors closest to catalytic site of the enzyme. Best poses were achieved  The inhibitory assay showed that PepTry and PepChy required a concentration approximately six and eight times higher than BTCI in order to result in total inhibition of trypsin and chymotrypsin, respectively. www.nature.com/scientificreports www.nature.com/scientificreports/ for both peptides, which occlude the enzyme catalytic pocket ( Fig. 4A-C), interacting with ΔG binding energies of about -11.0 kcal/mol. The majority of hydrogen bonds were observed between PepChy and ACE (Fig. 4B), involving three amino acid residues in PepChy (F3, S4 and I5) and six amino acid residues in ACE (R522, E411, Y523, H383, E384 and H353). For PepTry, the majority of hydrogen bonds were observed between two amino acid residues in PepTry (T2 and K3) and three amino acid residues in ACE (R522, E384 and A354). Moreover, electrostatic interaction was observed between PepTry (K3) and ACE (E384), with T-shapedπ-π-interactions between phenyl rings of Y523 in ACE and F3 in PepChy. It is noteworthy that amino acid residues H387, H383, E384 and E411 involved in coordination of Zinc in the native ACE active site also interact with PepChy and PepTry (Fig. 4B,C). Overall, the results showed that the interactions between ACE and the peptides occur within the catalytic site, leading to the complete inhibition of the enzyme, corroborating with the estimated IC 50 , as described above (Fig. 3).
In vivo assays. Cardiovascular and hemodynamic effects were evaluated in normotensive (Wistar, WR) and spontaneously hypertensive rats (SHR). Equimolar concentrations (3.31 mM) were employed for all molecules, with doses at 30.0 mg/kg for BTCI, 3.3 mg/kg for PepTry and PepChy), and 0.9% for NaCl (Vehicle) (Figs. 5A-D, 6A-D, 7A-D). As expected, the vehicle did not promote significant changes in any of the hemodynamic parameters. In contrast, all cardiovascular parameters were modified after treatment with BTCI and its derived peptides (Figs. 5A-D, 6A-D). The comparison of the maximum response to BTCI, PepChy and PepTry between normotensive and SHR is presented in Fig. 7A-D.
As shown in Figs. 5-7, values of hemodynamic parameters evaluated in WR rats in the presence of BTCI and derived peptides presented reductions of SBP and DBP. In contrast, values for RAC and AVC increased following administration of BTCI and peptides, with the greatest response for BTCI and PepChy at 180 minutes, and PepTry at 270 minutes. In the SHR rats, values of SBP and DBP were similar, with faster reduction for BTCI and PepChy, when compared toPepTry. In contrast, an increase in RAC and AVC hemodynamic parameters was observed after 30 minutes administration of BTCI and peptides, with greater expressiveness for PepChy.
The results presented in Fig. 6A-D indicate significant increases in the renal and aortic conductance for all molecules, up to 180 minutes, compatible with a vasodilation effect on the respective arteries. Indeed, comparing the WR and SHR models studied for BTCI, the measured values were similar up to 180 minutes differing from 180 to 360 minutes. At 360 minutes, the values for the WR rats drastically decreased near to the basal level. These data indicate that vasodilation of the renal artery was greater than for the aortic artery (Fig. 6A,C). The expressive increase of CVR and AVC for PepChy in both WR and SHR models is noteworthy (Fig. 6A-D). Altogether, the in vivo assays suggest that BTCI and related peptides resulted in a greater hypotensive effect in SHR than in WR rats ( Fig. 7A-D).

Effects of BTCI, PepChy and PepTry on left ventricular contractility and coronary vasodilation.
The effects of BTCI, PepChy and PepTry on left ventricular contractility and coronary vasomotricity in isolated hearts are presented in Fig. 8A-L. All molecules showed expressive changes in left ventricular contractility and coronary vasodilation.

Discussion
Hypertension is a worldwide health problem, considered a major risk factor for stroke cardiovascular morbidity and mortality, as well as renal diseases. Most classes of antihypertensive drugs, in addition to providing cardiovascular benefits, can prevent non-cardiovascular diseases such as glaucoma and diabetes 23 . Nevertheless, adverse side effects in some patients have been responsible for limitations and reduced compliance of current treatments 23,24,41 . The pharmacological potential of bioactive peptides from plants and synthetic peptides has been widely examined, with activity in number in the treatment of hypertension also explored [26][27][28][29][30][31] .Continued investigation of novel molecules, as natural, synthetic or modified compounds, has been recommended for drug development with therapeutic potential for treatment of hypertension and other cardiovascular diseases 25,30,31,42 . In the present study, hypotensive and vasodilator properties were investigated in BTCI and two newly synthesized www.nature.com/scientificreports www.nature.com/scientificreports/ peptides derived from BTCI, namely PepChy and PepTry. Additionally, potential mechanisms of action underlying hypotensive effects were evaluated.
BTCI is well-characterized as a "double-headed" protease inhibitor capable of inhibiting trypsin and chymotrypsin independently and simultaneously, irreversibly blocking the enzyme active site through its two specific reactive sites (P1), K26 and F53, respectively 14,[16][17][18][19]43,44 . Here, bioactive peptides derived from BTCI were designed to contain these reactive sites for trypsin (PepTry) and chymotrypsin (PepChy). These inhibited the proteases with less affinity than BTCI, as indicated by inhibition constants. Comparatively, the lower affinity of these peptides may be due to the lack of structural regions such as those stabilizing the whole interface of the BTCI-protease complex. This is in agreement with previous reported data in which cyclic peptides from the BBI family with additional amino acid residues after the disulfide bond presented a higher inhibitory activity against proteases 45,46 .
Bioactive peptides have been described as blood-pressure lowering agents by decreasing renin or angiotensin-converting enzyme (ACE) activities, and by regulating the NO synthesis pathway 27,28 . Among these, peptides with high antihypertensive potency have been designed based on the side chain amino acid cleavage position. Some of these containing charged (arginine and/or lysine), hydrophobic (valine, isoleucine, proline) and mainly aromatic amino acids (phenylalanine, tyrosine) residues, were identified as ACE inhibitors and characterized as potent antihypertensive agents [47][48][49] . A BIOPEP database of biologically active peptides, including the ACE-inhibitors, together with their amino acid sequences, is available at http://www.uwm.edu.pl/biochemia/ index.php/pl/biopep 48 . Nevertheless, to date, no cyclic peptides derived from BBIs such as BTCI, which contain reactive sites formed by aromatic and positive charged amino acid residues, have been investigated as antihypertensive agents.
Similarly to BTCI, the two cyclic peptides studied contain the same reactive sites K26 and F53 responsible for inhibiting trypsin and chymotrypsin, respectively and also trypsin-and chymotrypsin-like proteases. These peptides were chosen based on our previous studies, where the natriuretic, hemodynamic, cardiovascular and anticarcinogenic effects of BTCI were attributed to its ability to inhibit trypsin and chymotrypsin-like proteases 11,21,22,33 . Therefore, as expected, all results showed that blood pressure-lowering on WR and SHR is associated with the capability of BTCI and both peptides to inhibit proteases, such as ACE, and other trypsin/ chymotrypsin-like proteases. Therefore, here, the ACE-inhibitory activity of BTCI, PepChy and PepTry increased in a dose-dependent manner, presenting IC 50 values estimated at 54.6 ± 2.9 µM, 24.7 ± 1.1 µM, and 24.4 ± 1.1 µM, respectively.
The inhibition activities of BTCI and the peptides against ACE was performed in order to investigate the possible mechanism involved in blood pressure-lowering on WR and SHR, given that ACE is a protease involved in blood pressure control and BTCI and tested peptides are proteases inhibitors. As observed, maximum concentration of BTCI for inhibition of ACE were greater (300 µM) compared with trypsin and chymotrypsin inhibition (6 µM and 40 µM, respectively), indicating that BTCI is a more potent inhibitor against trypsin and chymotrypsin than ACE. Therefore, the differences in maximum concentration for inhibition of ACE by BTCI compared to www.nature.com/scientificreports www.nature.com/scientificreports/ those proteases can be attributed to conformational differences in the active site of ACE, leading to steric impediments that avoid or hinder optimal interaction between BTCI and ACE molecules.
Based on these results, the inhibitory activities of BTCI and peptides against ACE may be mainly due to the presence of the basic (lysine) and the hydrophobic (phenylalanine) amino acids in the reactive loops that also determine the potency of protein and peptide inhibition of trypsin and chymotrypsin proteases. In all three molecules, the positively charged ε-amine group derived from lysine and the phenylalanine side chain might have occluded the ACE active site. It is noteworthy that peptides containing aromatic, positively charged and some hydrophobic residues (proline, isoleucine, valine and leucine), such as BTCI, have been reported as strong ACE-inhibitors and antihypertensive agents 49 . Additionally, the IC 50 values of BTCI, PepChy and PepTry are in www.nature.com/scientificreports www.nature.com/scientificreports/ agreement with ACE-inhibitory peptides from several proteins deposited in the BIOPEP database (http://www. uwm.edu.pl/biochemia/index.php/en/biopep) 48 .
The ACE-inhibitory effects of PepChy and PepTry were consistent with molecular docking simulation. As seen in Fig. 4, the hydrophobic phenylalanine and the charged lysine amino acids in all the inhibitors make the most important hydrophobic and electrostatic interactions in the ACE active site and are responsible for inhibiting ACE activity.
The docking simulation showed that the interaction between the two peptides and ACE occurs with the key residues within the catalytic site. This consists of E411, Y523, V518, H353, H513, E384, A354, Y520, E384 and Zn 2+ . The stability of ACE peptides interaction is represented by the binding energy values, dependent on the number of hydrogen bonds, hydrophobic and electrostatic interactions, as well as interaction with Zn 2+ cofactor. Typically, high stability is related to a better inhibition showing lower IC 50 value. The best results were obtained for PepChy and PepTry at the ACE active site near to the Zn 2+ ion. The lowest values of binding energies and IC 50 of approximately -11.0 kcal/mol and 24.0 µM, respectively, were compatible with the high stability of the ACE-peptide complexes. The peptides bind to the active site pocket of ACE through hydrogen bonds, and hydrophobic and electrostatic forces. In addition, PepChy and PepTry are also linked through F3 and K3, respectively, with specific amino acid side chains responsible for zinc coordination, such as E411, H383 and E384, by induced ion-dipole and electrostatic interactions. It has been reported that the same interactions play an important role in stabilizing the interface of the ACE-lisinopril and selenium analogues of captopril complexes 38,40 . Therefore, these results suggested that these interactions contribute to the stabilization of the ACE-peptide complex, which lead to strong ACE-inhibitory activity. Together, these results highlight the important in vitro and in vivo antihypertensive properties and potential roles for these molecules in the reduction of blood pressure, as discussed below.
The present study was firstly motivated by the inhibitory effects of BTCI related to its natriuretic effects on the renal system model of Wistar rats. As reported, BTCI enhanced guanylin-induced natriuresis 21 and showed a protective effect on bradykinin towards plasma serine proteases cleavage as well as enhanced renal aortic vasodilation induced by bradykinin 22 . Furthermore, it is also known that the cyclic peptides with nine amino acid residues stabilised by one present disulfide bond, in addition to their inhibitory activity against trypsin and chymotrypsin, through lysine and phenylalanine reactive sites, respectively, also offer structural rigidity and stability and can be tested and used as a natural protease inhibitor 45,46 . Given the structural and inhibitory properties of BTCI and peptides, as well as the natriuretic and hemodynamic effects of BTCI, the hypotensive action through the peripheral vascular resistance and endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) (eNOS/NO) pathway in vivo and ex vivo, respectively, were investigated in the present study.
The results of in vivo assays showed: I. BTCI and related peptides promoted a decrease of SBP and DBP and renal and aortic vasodilation in WR and SHR; II. The decrease in SBP, DBP and aortic vasodilation promoted by BTCI and PepChy is more expressive in SHR than WR; III. The reduction in SBP, DBP and renal vasodilation caused by PepTry administration is more expressive in SHR than WR. Taken together, the results demonstrate that BTCI and related peptides have antihypertensive and vasodilator effects. These findings highlight the therapeutic potential of the compounds in the treatment of hypertension.
In general, the blood pressure-lowering effects of drugs, chemical compounds or bioactive peptides are related with ability to modulate the renin-angiotensin system by the inhibition of renin or angiotensin-converting enzyme (ACE), as well as due to increased enzymatic activity of the endothelial nitric oxide synthase (eNOS), leading to increased nitric oxide (NO) 27,28 . Antihypertensive drugs to reduce systolic or diastolic blood pressure are important in prevention of cardiovascular diseases and stroke. Our data demonstrate that BTCI and peptides administration promoted a decrease of SBP and DBP and an increase in RVC and AVC in WR and SHR. These increases indicate vasodilation in the renal and aortic territories, which possibly leads to the hypotension observed after oral administration of BTCI and the peptides. All the results are consistent with our previous data, which showed that BTCI has a protective effect on bradykinin cleavage through inhibition of plasma serine proteases, with vasodilation and hypotensive responses after intravenous administration 22 . It has also been demonstrated that BTCI enhanced the effect of guanylin on natriuresis, increasing urine flow, Na + excretion and glomerular filtration rate 21 . In addition, the greatest effects of guanylin on osmolar clearance were achieved in the presence of BTCI. It is noteworthy that the results obtained here are consistent with those reported for bioactive peptides, hydrolysed proteins and commercial compounds that reduce SBP and DBP and increase renal and aortic vasodilation 28,[49][50][51] . Altogether, these effects and the sodium and water/urine excretion (pressure-natriuresis) promoted by BTCI can provide a compensatory effect on reducing blood pressure by decreasing the peripheral vascular resistance [52][53][54] .
PepTry and PepChy also showed vascular relaxation and consequent hypotensive effects in vivo. We highlight the PepChy effects, since this peptide promoted expressive aortic vasodilation in SHR. Additionally, we demonstrated that hypotensive responses observed by peptide administration were more expressive in SHR than in WR. Therefore, these results indicate a possible therapeutic potential of BTCI and its related peptides in the treatment of hypertension.
The renin-angiotensin system (RAS) plays a crucial role in regulating blood pressure and electrolyte homeostasis. ACE catalyzes the degradation of the blood pressure lowering bradykinin that is responsible for the release of prostacyclin and nitric oxide (NO) on blood vessels 40,[55][56][57][58] . These systems were tested in ex vivo assays only for BTCI and PepChy, considering their higher expressive hypotensive effects in vivo compared to PepTry. Interestingly, no inhibitory effects of BTCI and its derived peptide, PepChy, were detected in the specific systems.
Nevertheless, BTCI and these peptides were able to promote an important coronary vasodilation, observed by the reduction in the perfusion pressure of the isolated hearts. These finding are also in agreement with the increase in the vascular conductance observed in in vivo assays. In addition, coronary vasodilation induced by BTCI or PepChy was completely blunted by the nitric oxide synthase inhibition. Pre-treatment with L-NAME, however, did not change the reduction in perfusion pressure induced by PepTry. Therefore, the coronary vasodilation www.nature.com/scientificreports www.nature.com/scientificreports/ induced by BTCI and PepChy is mediated by the eNOS/NO pathway regulating the vascular tonus of the coronary arteries and myocardial contractility [59][60][61][62][63][64] . In contrast, the coronary vasodilation induced by PepTry appears to be mediated in a nitric oxide-independent manner. Further investigation is required to better understand this effect. Additionally, we observed that BTCI and PepChy promoted a negative inotropic effect in isolated hearts that was inhibited by the non-selective NOS inhibitor, L-NAME. The pre-treatment with L-NAME was not able to blunt the effects of PepTry on cardiac contractility, suggesting again, that the nitric oxide is not involved in the effects evoked by this peptide.
In conclusion, the presented results showed that BTCI, PepChy and PepTry promoted reduction in blood pressure and coronary vasodilation. These data indicate great therapeutic potential in the peptides, forming the basis for further studies into the development of new drugs for treatment of cardiovascular diseases.

Material and methods
Purification of BTCI and its derived peptides, PepTry and PepChy. BTCI was purified from Vigna unguiculata seeds as previously described by Ventura et al. 13,65 . In brief, crude extracts (CE) were obtained through incubation of homogenized seeds in distilled water in the presence of 10.0 μM phenylmethanesulfonyl fluoride (PMSF), a synthetic protease inhibitor, at 4 °C for 12 hours, followed by precipitation with 2.5% (v/v) TCA (trichloroacetic acid PA-C 2 HCl 3 O 2 ) (Sigma Aldrich, USA) and 50% ammonium sulfate ((NH 4 ) 2 SO 4 ) (m/v). The suspension was centrifuged at 8.000 g for 40 minutes at 4 °C and the precipitate (CE) collected, dialyzed and stored at 4 °C. Purification of BTCI was achieved with one step of ion exchange chromatography using previously activated DEAE-cellulose resin (Sigma Aldrich). The proteins were eluted using 10 mM phosphate buffer, pH 7.3 and a linear gradient of 0-800 mM NaCl. The fractions corresponding to BTCI were dialyzed and lyophilized.
Peptides were purified by reverse-phase high performance liquid chromatography, RP-HPLC (Class LC-20A from Shimadzu Corp., Kyoto, Japan), using a semi-preparative C18 Shim-pak VP-ODS column (5 µm, 4.6 × 250 mm) (Shimadzu Corp., Kyoto, Japan) with a linear gradient (5-45%) of ACN. The column was washed with 95% ACN and 0.1% TFA and equilibrated with 5% ACN and 0.1% TFA. Crude peptide extract was applied to the column and subjected to a linear gradient (5 to 95%) of ACN for 90 minutes at a flow rate of 1.0 mL/min. The chromatographic profile was followed by reading the absorbance at 220 nm.
Purity and molecular mass of BTCI and peptides were estimated by electrospray ionization mass spectrometry (ESI-MS) on a LCMS-2020 system (Shimadzu Corp., Kyoto, Japan). The positive reflector with external calibration, as well as molecular mass range of calibrants (900-9100 Da), were considered. Concentration of the peptides was estimated using absorbance at 215, 225 and 205 nm, as follows 69 : (1)

225 205
Inhibition assay of BTCI, PepTry and PepChy against trypsin and chymotrypsin. The inhibitory activities of BTCI, PepTry and PepChy against trypsin and chymotrypsin activities were assayed using the substrates Na-benzoyl-DL-arginine-p-nitroanilide (BAPNA) and n-glutaryl-L-phenylalanine-p-nitroanilide (GPNA), respectively (Sigma) 19 ACE inhibition assay. Inhibitory activities of peptides PepTry and PepChy and BTCI against ACE were performed according to Hayakari, et al. (1978) using the synthetic substrate hippuryl-L-histidyl-L-leucine (HHL) 71 . Briefly, ACE stock solution was obtained from rabbit lung in 10 mM sodium phosphate buffer, pH 8.3, and BTCI and peptides stock solutions were prepared in MilliQ water (m/v). Concentration of BTCI was estimated from spectrophotometric absorbance at 280 nm based A 280 1% = 8.23. Concentration of PepTry and PepChy were estimated according to Eq. 1. BTCI and peptides were diluted in 10 mM sodium phosphate buffer pH 8.3 to obtain concentrations ranging from 0 to 600 µM. Ten µL peptide solutions were pre-incubated with 10 µL of ACE containing 0.5 M NaCl at 37 °C for 10 min. The mixture was then incubated with 10 µL substrate containing www.nature.com/scientificreports www.nature.com/scientificreports/ 12.5 mM HHL in 10 mM sodium phosphate buffer, pH 8.3 and 300 mM NaCl at 37 °C for 45 min. The enzymatic reaction was terminated by adding a mixture containing 550 µL of 100 mM sodium phosphate pH 8.3 and 332.5 µL of 3% (m/v) 2,4,6-trichloro-s-triazine (TT) (in dioxane). The mixture was incubated for 5 min. at room temperature and then centrifuged at 1000 g for 10 min at 4 °C. The absorbance values of supernatants containing hippuric acid liberated from HHL were recorded at 382 nm using the Jasco Spectrophotometer V-530 (Jasco Analytical Instruments, Tokyo, Japan). IC 50 values for peptides and BTCI were calculated from nonlinear regression curve of ACE residual activity (%) versus inhibitor concentration (µM) (BTCI, PepTry and PepChy) using Origin software (OriginLab Corporation, USA). The control was prepared containing only the substrate without enzyme solution. Assays were performed in triplicate.
Interaction of Peptides with Angiotensin converting enzyme (ACE) by docking procedure. The binding of peptides into the ACE active site was performed using the Evolutionary Algorithm for Docking (EADock) 72 , available at http://www.swissdock.ch/docking#. This algorithm is based on two adjust functions of molecular docking, interfaced with the CHARMM package for binding energy calculations and outputs the atomic coordinates of the protein-ligand complex. The tridimensional coordinates of target protein (ACE, PDB code 1O8A) and ligands, PepTry and PepChy, derived from BTCI structure (PDB code 3RU4), were considered for the docking procedure. The tridimensional coordinates of ligands were prepared using the Pymol program (DeLano Scientific LLC) and converted into a MOL2 file format with all hydrogen using the Open Babel GUI programa 73 version 2.4.1. The best model was ranked according to the position of ligands closest to the ACE active site, as well as the docking scores (Full Fitness) and the most favorable estimated Gibbs free energy values for the ACE-peptide binding process.

In vivo assays: hemodynamic and cardiovascular effects of BTCI, PepTry and PepChy.
Animals. Adult male Wistar (WR) and spontaneously hypertensive (SHR) rats weighing 250 to 300 g from the Department of Physiological Sciences of the Federal University of Goiás, Brazil, were employed in all in vivo assays. The animals were housed in a special room under controlled conditions of a 12-h light-dark cycle and at 23 ± 1 °C, with free access to water and food. The use of animals and the protocols for in vivo and ex vivo assays were approved by the Ethics Committee of Animal Use and Care at the Federal University of Goiás, Brazil (protocol numbers 13/2016 and 039/2017).
Surgical procedures. Surgical procedures were performed as previous described 19 . Rats were anesthetized with isoflurane (2% in O 2 ) and arterial blood flow (ABF) measurement was carried out from the right femoral or brachial artery via catheters. Administration of drugs was made through a catheter inserted into femoral veins. Anesthesia was maintained with urethane (1.2 mg.kg −1 , iv.). Tracheotomy was performed in order to reduce respiratory effort and airway resistance. The electrocardiogram (ECG) was monitored from needle electrodes on the forelimbs. For recording hemodynamic parameters rats were placed in a stereotaxic apparatus (Insight Ltda., Ribeirão Preto, SP, Brazil) in the ventral decubitus position. Body temperature was maintained at 37 ± 1.0 °C with a heated water-circulating pad.
Hemodynamic and cardiovascular parameters recording. After stabilization of the cardiovascular parameters, 0.9% NaCl (vehicle -V) = or BTCI (30.0 mg/kg) and its derived peptides, PepTry and PepChy (3.3 mg/kg) were administered via gavage. The hemodynamic and cardiovascular parameters, systolic blood pressure (SBP), diastolic blood pressure (DBP), renal vascular conductance (RVC) and aortic vascular conductance (AVC) were recorded immediately after gavage and were monitored during 6 hours.
Pulsatile arterial pressure (PAP) measurements were performed through an arterial cannula connected to a pressure transducer linked to a specific amplifier (MLT0380 and Bridge Amp, ML221, AD Instruments, Bella Vista, Australia). Hemodynamic parameters were recorded (1000 samples/s), using a digital analog converter and LabChart 7 software (PowerLab 4/25, ML845, AD Instruments, Bella Vista, Australia). ECG signals were recorded (2.000 samples/s) using a digital analog converter, and were considered to calculate HR. The RBF and ABF were recorded in absolute values (mL/min) through a miniature probe on the left renal artery and abdominal aorta connected to a T206 flowmeter (Transonic Systems, Inc., Ithaca, NY, USA). The recorded signals (1000 samples/s) were analyzed using the PowerLab 4/25, ML845, AD Instruments, Bella Vista, Australia). The RVC and AVC were estimated by the ratio of RBF/mean arterial pressure (MAP) and ABF/MAP, respectively. The ΔRVC and ΔAVC were expressed as percentage change.
Ex vivo assays: Effects of BTCI, PepChy and PepTry on left ventricular contractility and coronary vasodilation. Male WR and SHR weighing 250 to 300 g were employed as described above. To evaluate the cardiac effect of BTCI, PepChy and PepTry, isolated hearts were perfused according to the Langendorff technique 74 . Heparin (200 I.U) was injected intra-peritoneally and after 10-15 min animals were decapitated. The heart was carefully dissected and connected in the Langendorff system through the aortic stump and perfused with Krebs-Ringer solution containing 118.4 mM NaCl, 4.7 mM KCl, 1.2 mM KH 2 PO 4 , 1.2 mM MgSO 4 7H 2 O, 1.25 mM CaCl 2 2H 2 O, 11.7 mM glucose, and 26.5 mM NaHCO 3 . In addition, albumin (0.2 mg/mL) was added to Krebs Ringer's solution to prevent aggregation of BTCI and peptides in the glassware of the system. The system was maintained with constant perfusion flow of 10.0 ± 2 mL/min, at 37 °C and constant oxygenation (5% CO 2 and 95% O 2 ). Isovolumetric left ventricular systolic and diastolic pressures were measured after insertion of a balloon into the cavity of the left ventricle through a left atrium incision. Diastolic intraventricular pressure was measured during coronary perfusion via a pressure transducer connected to the aortic cannula and was adjusted to 10 ± 2 mmHg. After 30 to 40 min, the hearts were perfused for 10 minutes with KRS containing (1) BTCI (1.0 nM), (2) PepChy (1.0 nM), or (3) PepTry (1.0 nM). To evaluate the possible effects of these www.nature.com/scientificreports www.nature.com/scientificreports/ peptides on the eNOS/NO pathway, hearts were perfused with KRS containing nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME; 1 µM) for 15 min before the perfusion of BTCI, PepChy or PepTr 61 y. The coronary vasodilatations induced by peptides were recorded and analyzed using a data acquisition system (DATAQ Instruments, Akron, OH, USA). Statistical analysis. The GraphPad Prism software (v 6.03) was employed to calculate cardiovascular parameters as mean ± E.P.M (Mean Standard Error). Variance (two-way ANOVA) and a subsequent Tukey's post-test were used to analyze the SBP, DBP, RVC and AVC variations. The maximum response after administration of BTCI, PepChy and PepTry were analyzed using the Student's t-Test. The results obtained for left ventricular pressure from isolated hearts were compared using two-way ANOVA and Sidak's post-test. A value of p < 0.05 was considered to denote a significant difference.
Ethical approval and informed consent. All procedures were performed in accordance with institutional guidelines for the use of laboratory animals of the institution and the protocols were approved by the Ethics Committee of Animal Use of the Federal University of Goiás (protocol numbers 13/2016 and 039/2017).

Data availability
All data produced during this work are available from corresponding authors on reasonable request. www.nature.com/scientificreports www.nature.com/scientificreports/ www.nature.com/scientificreports www.nature.com/scientificreports/