Positive chemical compounds promote self-renewal capacity in the conversion of miPSCs into CSCs. (a) Sphere formation assay shows spherogenic potential and the expression of GFP. (b) Graphical representation of the number of spheroids after the conversion of 1-week. (c) Flow cytometric analysis shows GFP positive population in the conversion cells after treatment with chemical compounds. (d) The expression levels of stemness markers (endogenous genes) were analyzed by RT-qPCR. The data were analyzed using ordinary one-way ANOVA multiple comparisons and is presented as the mean ± standard deviation ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.