Virus shedding in oropharyngeal (OP) and cloacal (CL) swab samples after inoculation with homologous HPAIv. Titers of virus shed in OP and CL samples from chickens inoculated with inactivated vaccines (1, 0.1, or 0.01 dose, or sham-vaccinated), assessed at 3, 5, 7 and 10 days post-infection (dpi) with homologous HP H5 viruses. Viral titers are expressed as log10TCID50 (50% tissue culture infectious dose) in 0.1 ml, with error bars included. Vaccines were (A) rgKA435/184.108.40.206c in layer chickens, (B) rgES2/220.127.116.11c in layer chickens, (C) rgKA435/18.104.22.168c in breeder chickens, and (D) rgES2/22.214.171.124c in breeder chickens. The lower limit of detection was 1 log10TCID50 in 0.1 ml.