(A) Conjugation frequency of pK71-77-1-NDM from E. coli K71-77 into rifR K56 ECO-SENS strains. Vertical axis indicates the log10 of the conjugation frequency. (B) Relative fitness of rifR K56 ECO-SENS strains carrying pK71-77-1-NDM. Vertical axis indicates the fitness of plasmid-carrying strains relatively to their isogenic plasmid-free counterpart. (C) Stability of plasmid pK71-77-1-NDM in rifR K56 ECO-SENS strains. Vertical axis indicates the percentage of plasmid-carrying cells in the population. For all plots the horizontal axes indicate the rifR K56 strains’ suffix, and ST for non-ST73 strains; left panel: strains belonging to ST73, middle panel: strains belonging to other STs, right panel: overall comparison between ST73 and other STs. NS, ** and *** denote respectively: non-significant (p value > 0.05), p value < 0.01 and p value <0.001 (Wilcoxon test). The graphic illustrations were performed in R44.