Clinical validation and smart, connected health monitoring on the SCPT platform. (A) A process of on-chip nucleic acid extraction from different clinical samples. (B) Comparison of HPV 16 DNA quantitative detection in spiked saliva samples with: i) the SCPT platform and ii) qPCR method. (C) Quantitative detection of clinical vaginal swab samples by the SCPT platform and qPCR method. (D) Representative images of Pap smear results for negative clinical sample (clinical sample 1 in Fig. 4C) and positive clinical sample (clinical sample 9 in Fig. 4C). NILM and HSIL stand for High-Grade Squamous Intraepithelial Lesion and Negative for Intraepithelial Lesion or Malignancy, respectively. (E) Smart, connected disease monitoring and pathogen tracking by the SCPT platform.