Figure 6 | Scientific Reports

Figure 6

From: Enhanced tenacity of mycobacterial aerosols from necrotic neutrophils

Figure 6

Aerosols from mycobacterium-infected neutrophils. (a) Frequency of aerosol particles with certain aerodynamic diameters upon ultrawave nebulization of free mycobacterial cells in RPMI, RPMI alone, and mycobacterium-infected but untreated or H2O2-treated necrotic neutrophils (n = 3). (b) Distribution of optical diameters of aerosol particles generated by ultrawave nebulization from a mycobacterial suspension in RPMI, RPMI alone, or mycobacterium-infected but untreated or H2O2-treated necrotic neutrophils (n = 3). (c) Mycobacterial viability was assessed by CFU in aerosols collected from different levels of an Andersen impinger after nebulization using the ultrawave nebulizer for either mycobacterial cells alone or mycobacterium-infected but untreated or H2O2-treated necrotic neutrophils. Selected fractions containing airborne mycobacteria from necrotic neutrophils (PMNs) were further analysed by SEM (below) (n = 3, *p > 0.001).

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